本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表...本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表明,山药BAM1基因全长2066 bp,有BAMs家族基因的保守结构域Glyco_hydro_14,与几内亚薯蓣亲缘关系较近;该基因编码蛋白定位于细胞膜;在2个山药品种中,BAM1基因的表达量均在块茎膨大的前期和后期处于较高水平;通过酵母双杂交筛选出17个与其互作的蛋白,表达分析结果表明BAM1基因及其互作基因GEM-like Protein 5、TIFY10a-like和CXXS1响应植物的低温、干旱和盐胁迫。展开更多
To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The ex...To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.展开更多
文摘本研究以‘毕克齐’和‘大和长芋’山药块茎为试验材料,通过RACE技术克隆山药BAM1基因,并进行生物信息学分析与亚细胞定位;分析BAM1在山药块茎不同发育时期的表达模式;筛选其互作蛋白,并研究其在低温、干旱和盐胁迫下的表达特征。结果表明,山药BAM1基因全长2066 bp,有BAMs家族基因的保守结构域Glyco_hydro_14,与几内亚薯蓣亲缘关系较近;该基因编码蛋白定位于细胞膜;在2个山药品种中,BAM1基因的表达量均在块茎膨大的前期和后期处于较高水平;通过酵母双杂交筛选出17个与其互作的蛋白,表达分析结果表明BAM1基因及其互作基因GEM-like Protein 5、TIFY10a-like和CXXS1响应植物的低温、干旱和盐胁迫。
基金supported by the Wencheng County Science and Technology Plan Project(2023NKY03)Earmarked Fund for Modern Agro-industry Technology Research System(Grant Number CARS-24-B04,CARS-23-B05)Additional support was provided by Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Vegetables),Ministry of Agriculture and Rural Affairs,China.
文摘To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.
