Temperature is an important environmental factor affecting heading date of rice.Despite its importance,genes responsible for temperature-sensitive heading in rice have remained elusive.Our previous study identified a ...Temperature is an important environmental factor affecting heading date of rice.Despite its importance,genes responsible for temperature-sensitive heading in rice have remained elusive.Our previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been verified.In this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in ZS97.Experiments conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of OsMADS51.One-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene Ehd1.We further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature sensitivity.We also found that OsMADS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature sensitivity.These insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.展开更多
山东北部沿海平原区小麦-玉米周年两吨粮超高产技术创建是中国农业大学作物化控团队和莱州市金海种业在山东省莱州市开展的一项重要农业生产示范项目。通过精选良种、合理密植及水肥一体化等措施,以期实现小麦和玉米的高产高效种植。在2...山东北部沿海平原区小麦-玉米周年两吨粮超高产技术创建是中国农业大学作物化控团队和莱州市金海种业在山东省莱州市开展的一项重要农业生产示范项目。通过精选良种、合理密植及水肥一体化等措施,以期实现小麦和玉米的高产高效种植。在2023年该地块小麦和玉米两季的种植中,现场实测每公顷的总产量达到了31,323.90 kg(折合2088.26 kg mu-1)。其中冬小麦由农业农村部组织专家测产打破了黄淮海地区小麦的单产纪录,实际产量高达13,213.35 kg hm-2(折合880.89 kg mu-1)。种植品种烟农1212每平方米穗数为692.0,穗粒数为40.1,千粒重为52.55 g,单株产量为4.74 g,收获指数为0.56,水分利用效率为3.24 kg m-3。夏玉米中金玉2513的每平方米穗数为9.1,穗粒数为584.7,千粒重为379.31 g,单株产量为206.25 g,收获指数为0.58,水分利用效率为2.86 kg m-3,实际产量为18,110.55 kg hm-2(折合1207.37 kg mu-1)。在2024年的重复试验中,小麦-玉米的实际产量继续达到30,997.00 kg hm-2(折合2065.13 kg mu-1)。本文旨在总结高产纪录小麦-玉米复种高产田生产过程的主要管理环节和高产群体的主要指标,为山东北部沿海平原区小麦-玉米复种高产提供参考。展开更多
基金supported by the Laboratory of Lingnan Modern Agriculture Project(NT2021001)Guangdong Province Basic and Applied Basic Research Fund-Provincial and Municipal Joint Fund Project(2023A1515110882)+3 种基金Guangzhou Science and Technology Plan Project(2023A04J0811)Guangdong Province Rural Revitalization Strategy Special Fund Seed Industry Revitalization Project(2022-NPY-00-013)Natural Science Foundation of Zhejiang Pro-vince(LY22C130006)Key Laboratory of New Rice Breeding Technologies in Guangdong Province(2023B1212060042).
文摘Temperature is an important environmental factor affecting heading date of rice.Despite its importance,genes responsible for temperature-sensitive heading in rice have remained elusive.Our previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been verified.In this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in ZS97.Experiments conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of OsMADS51.One-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene Ehd1.We further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature sensitivity.We also found that OsMADS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature sensitivity.These insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.
文摘山东北部沿海平原区小麦-玉米周年两吨粮超高产技术创建是中国农业大学作物化控团队和莱州市金海种业在山东省莱州市开展的一项重要农业生产示范项目。通过精选良种、合理密植及水肥一体化等措施,以期实现小麦和玉米的高产高效种植。在2023年该地块小麦和玉米两季的种植中,现场实测每公顷的总产量达到了31,323.90 kg(折合2088.26 kg mu-1)。其中冬小麦由农业农村部组织专家测产打破了黄淮海地区小麦的单产纪录,实际产量高达13,213.35 kg hm-2(折合880.89 kg mu-1)。种植品种烟农1212每平方米穗数为692.0,穗粒数为40.1,千粒重为52.55 g,单株产量为4.74 g,收获指数为0.56,水分利用效率为3.24 kg m-3。夏玉米中金玉2513的每平方米穗数为9.1,穗粒数为584.7,千粒重为379.31 g,单株产量为206.25 g,收获指数为0.58,水分利用效率为2.86 kg m-3,实际产量为18,110.55 kg hm-2(折合1207.37 kg mu-1)。在2024年的重复试验中,小麦-玉米的实际产量继续达到30,997.00 kg hm-2(折合2065.13 kg mu-1)。本文旨在总结高产纪录小麦-玉米复种高产田生产过程的主要管理环节和高产群体的主要指标,为山东北部沿海平原区小麦-玉米复种高产提供参考。
