番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)严重威胁番茄等茄科园艺作物的生产安全。本研究根据其外壳蛋白(coat protein,CP)基因及其同属病毒的差异序列,设计了特异性重组酶介导等温核酸扩增技术(recombinase-aided ...番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)严重威胁番茄等茄科园艺作物的生产安全。本研究根据其外壳蛋白(coat protein,CP)基因及其同属病毒的差异序列,设计了特异性重组酶介导等温核酸扩增技术(recombinase-aided amplification,RAA)引物,并基于CRISPR/Cas12a的设计原则,设计了靶向RT-RAA扩增产物的CRISPR RNA(crRNA)。通过优化获得了检测信号最强的反应体系,其中报告基因FQ终浓度为600nmol/L、Cas12a和crRNA终浓度分别为200nmol/L和1000nmol/L,最终总反应时间仅为30 min。该方法可特异性检测ToBRFV,对携带ToBRFV的番茄样品RNA检测灵敏度为RT-PCR和RT-qPCR的10000和100倍,检测限为3.46fg/μL。阳性样品验证结果显示,本研究建立的RT-RAA-CRISPR/Cas12a检测技术可以在不同来源的辣椒、番茄侵染的植物叶片、果实及种子中检测到ToBRFV,表明该技术可用于番茄褐色皱纹果病毒的快速、灵敏的可视化检测。展开更多
Baraeus orientalis Aurivillius,1907 is classified within the family Cerambycidae(Lamiinae:Niphonini).It is found along the East to West coasts of Mid-Africa and has been recorded in Cameroon,Ethiopia,Kenya,Malawi,Nami...Baraeus orientalis Aurivillius,1907 is classified within the family Cerambycidae(Lamiinae:Niphonini).It is found along the East to West coasts of Mid-Africa and has been recorded in Cameroon,Ethiopia,Kenya,Malawi,Namibia,Somalia,Tanzania,Zambia and Zimbabwe(Teocchi,1997;Adlbauer,2000,2001;Tavakilian&Chevillotte,2023).The type locality of B.orientalis is Kigonsera village in Tanzania.Its host plant has remained unknown until the present discovery.Adenia globosa Engler,1891,a succulent plant belonging to the family Passifloraceae,is renowned for its medicinal properties and aesthetic value.The interception of B.orientalis on A.globosa represents a novel finding.展开更多
Maize chlorotic dwarf virus （MCDV） is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification （R...Maize chlorotic dwarf virus （MCDV） is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification （RT-LAMP） was estab- lished in this study. Based on the sequence of MCDV coat protein coding gene, specific primers were designed and similar sensitivities were observed between RT- LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescent display in daylight allows naked easy detection of the amplification of MCDV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCDV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter.展开更多
卷积神经网络模型可通过作物病害图像准确率较高地识别作物病害类型,达到防治作物病害的目的,但传统卷积神经网络模型存在模型尺寸大、迁移效果差等问题。针对这些问题,引入学习率动态衰减训练策略,使用EfficientNetV2的Fused-MBConv和M...卷积神经网络模型可通过作物病害图像准确率较高地识别作物病害类型,达到防治作物病害的目的,但传统卷积神经网络模型存在模型尺寸大、迁移效果差等问题。针对这些问题,引入学习率动态衰减训练策略,使用EfficientNetV2的Fused-MBConv和MBConv模块替换ResNet18的部分残差模块,提出Res-Efficient模型。实验证明,使用学习率动态衰减策略能提高Res-Efficient模型识别作物病害的准确率,Res-Efficient模型在Plant Village和2018 AI Challenger测试集上分别达到99.70%和87.20%的准确率,模型尺寸减少到14.0 MB。Res-Efficient模型能为移动端和嵌入式设备部署作物病害自动识别应用提供参考。展开更多
文摘番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)严重威胁番茄等茄科园艺作物的生产安全。本研究根据其外壳蛋白(coat protein,CP)基因及其同属病毒的差异序列,设计了特异性重组酶介导等温核酸扩增技术(recombinase-aided amplification,RAA)引物,并基于CRISPR/Cas12a的设计原则,设计了靶向RT-RAA扩增产物的CRISPR RNA(crRNA)。通过优化获得了检测信号最强的反应体系,其中报告基因FQ终浓度为600nmol/L、Cas12a和crRNA终浓度分别为200nmol/L和1000nmol/L,最终总反应时间仅为30 min。该方法可特异性检测ToBRFV,对携带ToBRFV的番茄样品RNA检测灵敏度为RT-PCR和RT-qPCR的10000和100倍,检测限为3.46fg/μL。阳性样品验证结果显示,本研究建立的RT-RAA-CRISPR/Cas12a检测技术可以在不同来源的辣椒、番茄侵染的植物叶片、果实及种子中检测到ToBRFV,表明该技术可用于番茄褐色皱纹果病毒的快速、灵敏的可视化检测。
基金supported by a start-up fund from Mianyang Normal University to Meiying Lin(QD2023A30)。
文摘Baraeus orientalis Aurivillius,1907 is classified within the family Cerambycidae(Lamiinae:Niphonini).It is found along the East to West coasts of Mid-Africa and has been recorded in Cameroon,Ethiopia,Kenya,Malawi,Namibia,Somalia,Tanzania,Zambia and Zimbabwe(Teocchi,1997;Adlbauer,2000,2001;Tavakilian&Chevillotte,2023).The type locality of B.orientalis is Kigonsera village in Tanzania.Its host plant has remained unknown until the present discovery.Adenia globosa Engler,1891,a succulent plant belonging to the family Passifloraceae,is renowned for its medicinal properties and aesthetic value.The interception of B.orientalis on A.globosa represents a novel finding.
文摘Maize chlorotic dwarf virus （MCDV） is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification （RT-LAMP） was estab- lished in this study. Based on the sequence of MCDV coat protein coding gene, specific primers were designed and similar sensitivities were observed between RT- LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescent display in daylight allows naked easy detection of the amplification of MCDV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCDV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter.
文摘卷积神经网络模型可通过作物病害图像准确率较高地识别作物病害类型,达到防治作物病害的目的,但传统卷积神经网络模型存在模型尺寸大、迁移效果差等问题。针对这些问题,引入学习率动态衰减训练策略,使用EfficientNetV2的Fused-MBConv和MBConv模块替换ResNet18的部分残差模块,提出Res-Efficient模型。实验证明,使用学习率动态衰减策略能提高Res-Efficient模型识别作物病害的准确率,Res-Efficient模型在Plant Village和2018 AI Challenger测试集上分别达到99.70%和87.20%的准确率,模型尺寸减少到14.0 MB。Res-Efficient模型能为移动端和嵌入式设备部署作物病害自动识别应用提供参考。
