为获取粗茎鳞毛蕨(Dryopteris crassirhizoma)的转录组信息并分析其基因表达特征,本研究采用Illumina HiSeq TM 2000150PE高通量测序平台,对其叶、叶柄、叶轴、根和根状茎进行转录组测序分析。结果表明:经Trinity拼接并去冗余后,共获得1...为获取粗茎鳞毛蕨(Dryopteris crassirhizoma)的转录组信息并分析其基因表达特征,本研究采用Illumina HiSeq TM 2000150PE高通量测序平台,对其叶、叶柄、叶轴、根和根状茎进行转录组测序分析。结果表明:经Trinity拼接并去冗余后,共获得147690个unigenes,在核酸序列库(Nucleotide sequence database,NT)、非冗余蛋白质数据库(Non Redundant protein database,NR)、Swiss Prot蛋白质序列数据库(Swiss Prot protein sequence database,Swiss Prot)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源蛋白质群数据库(Clusters of Orthologous Groups of proteins,COG)和基因本体库(Gene Ontology,GO)中获得注释的unigenes数量分别为91758、57578、60535、56670、41446、63027个。GO分类注释得到3大类56个分支,KEGG代谢通路注释到129条标准通路,其中23条为次生代谢通路。蛋白质编码框序列94731个,以短序列为主。简单重复序列(Simple Sequence Repeat,SSR)分析发现28079个SSRs,以二核苷酸SSRs最多,占总SSRs数量的70.12%。单核苷酸多态性(Single Nucleotide Polymorphism,SNP)分析检测到叶、叶柄、叶轴、根和根状茎的SNP数量分别为23139、21246、28280、55409、17084个。叶柄与根状茎差异基因表达量分析显示上调基因13829个、下调基因24250个。GO功能富集分析结果表明,在叶柄和根状茎组织中共富集到226个与苯丙烷类代谢途径相关的差异表达基因。本研究结果可为后续深入探究粗茎鳞毛蕨次生代谢通路、间苯三酚类与黄酮类等物质的生物合成及基因功能提供转录组数据支持。展开更多
Rutin,also known as vitamin P,belongs to the flavonoid class of compounds and is widely present in plants.Its ability to bind with albumin in the blood helps maintain capillary permeability,leading to its extensive us...Rutin,also known as vitamin P,belongs to the flavonoid class of compounds and is widely present in plants.Its ability to bind with albumin in the blood helps maintain capillary permeability,leading to its extensive use for cardiovascular protection.This review aimed to provide insights into the development of rutin raw material industry in China and its future applications in various fields,such as medicine,healthcare,food,and animal husbandry.The study began by comparing rutin quality standards across China,the United States and Europe,outlined the industrial extraction processes of rutin,and examined the biological activity and potential medical applications of rutin.展开更多
文摘为获取粗茎鳞毛蕨(Dryopteris crassirhizoma)的转录组信息并分析其基因表达特征,本研究采用Illumina HiSeq TM 2000150PE高通量测序平台,对其叶、叶柄、叶轴、根和根状茎进行转录组测序分析。结果表明:经Trinity拼接并去冗余后,共获得147690个unigenes,在核酸序列库(Nucleotide sequence database,NT)、非冗余蛋白质数据库(Non Redundant protein database,NR)、Swiss Prot蛋白质序列数据库(Swiss Prot protein sequence database,Swiss Prot)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源蛋白质群数据库(Clusters of Orthologous Groups of proteins,COG)和基因本体库(Gene Ontology,GO)中获得注释的unigenes数量分别为91758、57578、60535、56670、41446、63027个。GO分类注释得到3大类56个分支,KEGG代谢通路注释到129条标准通路,其中23条为次生代谢通路。蛋白质编码框序列94731个,以短序列为主。简单重复序列(Simple Sequence Repeat,SSR)分析发现28079个SSRs,以二核苷酸SSRs最多,占总SSRs数量的70.12%。单核苷酸多态性(Single Nucleotide Polymorphism,SNP)分析检测到叶、叶柄、叶轴、根和根状茎的SNP数量分别为23139、21246、28280、55409、17084个。叶柄与根状茎差异基因表达量分析显示上调基因13829个、下调基因24250个。GO功能富集分析结果表明,在叶柄和根状茎组织中共富集到226个与苯丙烷类代谢途径相关的差异表达基因。本研究结果可为后续深入探究粗茎鳞毛蕨次生代谢通路、间苯三酚类与黄酮类等物质的生物合成及基因功能提供转录组数据支持。
基金Supported by the Scientific and Technological Research Project Foundation of Henan Provincial Scientific and Technological Department(242102310530,242102310119,252102310498)the Zhumadian Science and Technology Innovation Youth Special Project(QNZX202413)+1 种基金the Henan Provincial Medical Science and Technology Tackling Program(LHGJ20241009,LHGJ20241006)the Key Scientific Research Project of Higher Education Institutions in Henan Province(25B180015,25B230006)。
文摘Rutin,also known as vitamin P,belongs to the flavonoid class of compounds and is widely present in plants.Its ability to bind with albumin in the blood helps maintain capillary permeability,leading to its extensive use for cardiovascular protection.This review aimed to provide insights into the development of rutin raw material industry in China and its future applications in various fields,such as medicine,healthcare,food,and animal husbandry.The study began by comparing rutin quality standards across China,the United States and Europe,outlined the industrial extraction processes of rutin,and examined the biological activity and potential medical applications of rutin.
