城市社区作为居民生活的基本单元,随着绿化率的提升,其也逐渐成为花粉致敏风险的突出区域之一,但当前对其致敏状况认识还十分薄弱。选取广州市4种典型城市社区花粉致敏植物为研究对象,利用城市绿地致敏指数(Urban Green Zone Allergenic...城市社区作为居民生活的基本单元,随着绿化率的提升,其也逐渐成为花粉致敏风险的突出区域之一,但当前对其致敏状况认识还十分薄弱。选取广州市4种典型城市社区花粉致敏植物为研究对象,利用城市绿地致敏指数(Urban Green Zone Allergenicity Index,I_(UGZA))对社区尺度花粉致敏潜力进行评估,以探讨社区植物致敏风险特征及其关键影响因子。研究结果表明:1)调查记录到4种典型城市社区有823棵树木,涵盖了33科45属52种,其中,致敏植物有16科20属24种,以本地物种为主,占比66.7%,外来物种占比33.3%。2)4种典型城市社区的I_(UGZA)均值为0.1947,致敏潜力较低,低于北京和成都的平均水平,且春季的致敏风险最高,远大于其他季节。3)植被结构、物种重要值(IV)和树木密度与I_(UGZA)值呈极显著正相关(P≤0.01),表明它们对致敏风险有着重要影响。展开更多
Mammalian sterile-20-like kinase 1(MST1)is a core component of the Hippo signaling pathway.A previous study of 24 patients with MST1 deficiency revealed that more than half of the patients presented symptoms of airway...Mammalian sterile-20-like kinase 1(MST1)is a core component of the Hippo signaling pathway.A previous study of 24 patients with MST1 deficiency revealed that more than half of the patients presented symptoms of airway hyperresponsiveness and atopic dermatitis.We also found significantly reduced MST1 expression in patients with allergies and in mouse models of allergic asthma,suggesting that aberrant MST1 expression may be broadly relevant to allergic diseases.However,the specific mechanism by which MST1 may be related to allergic disorders has remained unclear.In our study,Mst1^(-/-)mice displayed exacerbated IgE-mediated allergic responses,including passive systemic and cutaneous anaphylaxis.More intriguingly,mast cell-deficient Kit^(W-sh/W-sh) mice reconstituted with Mst1^(-/-)bone marrow-derived mast cells(BMMCs)also presented aggravated IgE-mediated hypersensitivity reactions and mast cell-dependent asthma.MST1 deficiency notably promoted inflammatory cytokine production,cell degranulation,and intracellular calcium mobilization in FcεRI-stimulated BMMCs.Mechanistically,MST1 facilitates SRC homology domain-containing tyrosine phosphatase-1(SHP-1)-mediated dephosphorylation of LCK/YES-related protein tyrosine kinase(LYN)at Y397 to repress FcɛRI signaling.Coimmunoprecipitation studies revealed that MST1 acts as a scaffold molecule to enhance the interaction between SHP-1 and LYN in a kinase activity-independent manner.Two patient-derived mutants presented significantly reduced intracellular protein expression levels and impaired LYN-SHP-1 interactions.Our study reveals a noncanonical role of MST1 in maintaining immune homeostasis by preventing mast cell-mediated hypersensitivity.This likely explains the increased susceptibility to allergic diseases in MST1-deficient patients.展开更多
文摘城市社区作为居民生活的基本单元,随着绿化率的提升,其也逐渐成为花粉致敏风险的突出区域之一,但当前对其致敏状况认识还十分薄弱。选取广州市4种典型城市社区花粉致敏植物为研究对象,利用城市绿地致敏指数(Urban Green Zone Allergenicity Index,I_(UGZA))对社区尺度花粉致敏潜力进行评估,以探讨社区植物致敏风险特征及其关键影响因子。研究结果表明:1)调查记录到4种典型城市社区有823棵树木,涵盖了33科45属52种,其中,致敏植物有16科20属24种,以本地物种为主,占比66.7%,外来物种占比33.3%。2)4种典型城市社区的I_(UGZA)均值为0.1947,致敏潜力较低,低于北京和成都的平均水平,且春季的致敏风险最高,远大于其他季节。3)植被结构、物种重要值(IV)和树木密度与I_(UGZA)值呈极显著正相关(P≤0.01),表明它们对致敏风险有着重要影响。
基金supported by grants from the National Natural Science Foundation of China(8187060308,U22A20307,and 81930041)the Key R&D Program of Zhejiang Province(2024C03177)+1 种基金the Noncommunicable Chronic Diseases-National Science and Technology Major Project(2024ZD0541200)the Natural Science Foundation of Zhejiang Province(LZ24H100001).
文摘Mammalian sterile-20-like kinase 1(MST1)is a core component of the Hippo signaling pathway.A previous study of 24 patients with MST1 deficiency revealed that more than half of the patients presented symptoms of airway hyperresponsiveness and atopic dermatitis.We also found significantly reduced MST1 expression in patients with allergies and in mouse models of allergic asthma,suggesting that aberrant MST1 expression may be broadly relevant to allergic diseases.However,the specific mechanism by which MST1 may be related to allergic disorders has remained unclear.In our study,Mst1^(-/-)mice displayed exacerbated IgE-mediated allergic responses,including passive systemic and cutaneous anaphylaxis.More intriguingly,mast cell-deficient Kit^(W-sh/W-sh) mice reconstituted with Mst1^(-/-)bone marrow-derived mast cells(BMMCs)also presented aggravated IgE-mediated hypersensitivity reactions and mast cell-dependent asthma.MST1 deficiency notably promoted inflammatory cytokine production,cell degranulation,and intracellular calcium mobilization in FcεRI-stimulated BMMCs.Mechanistically,MST1 facilitates SRC homology domain-containing tyrosine phosphatase-1(SHP-1)-mediated dephosphorylation of LCK/YES-related protein tyrosine kinase(LYN)at Y397 to repress FcɛRI signaling.Coimmunoprecipitation studies revealed that MST1 acts as a scaffold molecule to enhance the interaction between SHP-1 and LYN in a kinase activity-independent manner.Two patient-derived mutants presented significantly reduced intracellular protein expression levels and impaired LYN-SHP-1 interactions.Our study reveals a noncanonical role of MST1 in maintaining immune homeostasis by preventing mast cell-mediated hypersensitivity.This likely explains the increased susceptibility to allergic diseases in MST1-deficient patients.
