目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和...目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。展开更多
BACKGROUND The incidence of diabetic atherosclerosis(DMA)is increasing worldwide,but its pathogenesis remains incompletely understood.In addition to cardiovascular complications,bladder dysfunction is one of the commo...BACKGROUND The incidence of diabetic atherosclerosis(DMA)is increasing worldwide,but its pathogenesis remains incompletely understood.In addition to cardiovascular complications,bladder dysfunction is one of the common comorbidities associated with DMA but is often refractory to current treatments.AIM To investigate the therapeutic effect of human amniotic fluid stem cell-derived extracellular vesicles(hAFSC-EVs)on the recovery of bladder dysfunction in DMA rats.METHODS Eighty rats were divided into normal control,streptozotocin-induced diabetic rats,diabetic rats subjected to arterial balloon endothelial injury of common iliac artery(DMA),and DMA rats treated with hAFSC-EVs(DMA+hAFSC-EVs).At 4 weeks and 12 weeks after DMA induction,levels of blood glucose,total cholesterol,triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,homeostasis model assessment(HOMA)-insulin resistance,and HOMA-βwere measured.Cystometry,common iliac artery wall thickness,and bladder tumor necrosis factor(TNF)-α,interleukin(IL)-6,transforming growth factor(TGF)-β1,Smad3,connective tissue growth factor(CTGF)and fibronectin were also evaluated.RESULTS Bladder weight and blood glucose,triglyceride,HOMA-insulin resistance,common iliac artery intima thickness,voided volume,intercontraction interval,bladder capacity,and mRNA expression of TNF-α,IL-6,TGF-β1,Smad3,CTGF and fibronectin were significantly increased at 4 weeks and 12 weeks after induction,while the HOMA-βlevel decreased at 4 weeks and 12 weeks,and the high-density lipoprotein cholesterol level decreased at 12 weeks.hAFSC-EVs treatment in DMA rats significantly reduced bladder weight and blood glucose,thickness of common iliac arterial intima,voided volume,intercontraction interval and bladder capacity at 4 weeks.The mRNA expression of TNF-α,TGF-β1,and CTGF in DMA rats treated with hAFSC-EVs were significantly decreased at 4 weeks,while the mRNA expressions of IL-6 and Smad3 were significantly decreased 12 weeks.CONCLUSION hAFSC-EVs treatment can help restore DMA-induced bladder dysfunction,which is associated with lowered blood glucose levels,reduced arterial wall thickness,and decreased TNF-α,IL-6,TGF-β1,Smad3,and CTGF expression.展开更多
目的探究德谷胰岛素利拉鲁肽注射液(insulin degludec/liraglutide,IDegLira)对2型糖尿病(T2DM)合并颈动脉斑块患者的临床疗效。方法86例T2DM合并颈动脉斑块患者,随机分为对照组和观察组,每组43例。试验过程中退出3例,其中对照组41例完...目的探究德谷胰岛素利拉鲁肽注射液(insulin degludec/liraglutide,IDegLira)对2型糖尿病(T2DM)合并颈动脉斑块患者的临床疗效。方法86例T2DM合并颈动脉斑块患者,随机分为对照组和观察组,每组43例。试验过程中退出3例,其中对照组41例完成试验,观察组42例完成试验。对照组给予德谷胰岛素、二甲双胍片联合他汀类药物治疗,同时给予饮食运动教育;观察组将德谷胰岛素替换为IDegLira,其余治疗不变。比较两组受试者治疗前后血脂、血糖、体重指数(BMI)、腰围以及颈动脉内膜中层厚度(IMT)、颈动脉斑块面积及胰岛素用量、不良反应发生情况。结果治疗后,两组IMT、颈动脉斑块面积均较基线改善,且干预组IMT(0.98±0.42)mm和颈动脉斑块面积(16.48±9.68)mm^(2)均小于对照组的(1.21±0.36)mm、(21.21±10.87)mm^(2)(P<0.05)。治疗后,两组空腹血糖(FPG)、餐后2小时血糖(2 h PG)、糖化血红蛋白(HbA1c)水平均低于本组治疗前,且观察组FPG(5.98±1.82)mmol/L、2 h PG(7.48±2.68)mmol/L、HbA1c(6.80±1.24)%均低于对照组的(7.21±1.56)mmol/L、(9.54±4.87)mmol/L、(8.55±2.27)%(P<0.05)。治疗后,两组总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平均低于本组治疗前,且观察组TC(3.92±0.89)mmol/L、LDL-C(2.80±1.24)mmol/L均低于对照组的(5.14±0.92)、(3.55±1.27)mmol/L(P<0.05);观察组甘油三酯水平低于本组治疗前(P<0.05)。治疗后,观察组腰围、BMI均低于本组治疗前,且观察组腰围(91.68±7.28)cm、BMI(23.26±1.36)kg/m^(2)均低于对照组的(95.24±8.23)cm、(25.74±2.48)kg/m^(2)(P<0.05);观察组胰岛素用量(18.67±5.26)U/d少于对照组的(22.65±6.76)U/d(P<0.05)。两组不良反应发生率比较无明显统计学差异(P>0.05)。结论IDegLira能显著改善T2DM合并颈动脉斑块患者的血管条件,缩小斑块面积,降低心脑血管发病风险,进一步改善患者血糖、血脂水平,同时能减重控制腰围,且不增加药物不良反应,可作为T2DM合并颈动脉斑块患者的优选。展开更多
文摘目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。
基金the Ministry of Science and Technology Taiwan,No.MOST 109-2314-B-182A-091,No.NSTC 112-2314-B-182A-062, No.NSTC 113-2314-B-182A-125.
文摘BACKGROUND The incidence of diabetic atherosclerosis(DMA)is increasing worldwide,but its pathogenesis remains incompletely understood.In addition to cardiovascular complications,bladder dysfunction is one of the common comorbidities associated with DMA but is often refractory to current treatments.AIM To investigate the therapeutic effect of human amniotic fluid stem cell-derived extracellular vesicles(hAFSC-EVs)on the recovery of bladder dysfunction in DMA rats.METHODS Eighty rats were divided into normal control,streptozotocin-induced diabetic rats,diabetic rats subjected to arterial balloon endothelial injury of common iliac artery(DMA),and DMA rats treated with hAFSC-EVs(DMA+hAFSC-EVs).At 4 weeks and 12 weeks after DMA induction,levels of blood glucose,total cholesterol,triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,homeostasis model assessment(HOMA)-insulin resistance,and HOMA-βwere measured.Cystometry,common iliac artery wall thickness,and bladder tumor necrosis factor(TNF)-α,interleukin(IL)-6,transforming growth factor(TGF)-β1,Smad3,connective tissue growth factor(CTGF)and fibronectin were also evaluated.RESULTS Bladder weight and blood glucose,triglyceride,HOMA-insulin resistance,common iliac artery intima thickness,voided volume,intercontraction interval,bladder capacity,and mRNA expression of TNF-α,IL-6,TGF-β1,Smad3,CTGF and fibronectin were significantly increased at 4 weeks and 12 weeks after induction,while the HOMA-βlevel decreased at 4 weeks and 12 weeks,and the high-density lipoprotein cholesterol level decreased at 12 weeks.hAFSC-EVs treatment in DMA rats significantly reduced bladder weight and blood glucose,thickness of common iliac arterial intima,voided volume,intercontraction interval and bladder capacity at 4 weeks.The mRNA expression of TNF-α,TGF-β1,and CTGF in DMA rats treated with hAFSC-EVs were significantly decreased at 4 weeks,while the mRNA expressions of IL-6 and Smad3 were significantly decreased 12 weeks.CONCLUSION hAFSC-EVs treatment can help restore DMA-induced bladder dysfunction,which is associated with lowered blood glucose levels,reduced arterial wall thickness,and decreased TNF-α,IL-6,TGF-β1,Smad3,and CTGF expression.
文摘目的探究德谷胰岛素利拉鲁肽注射液(insulin degludec/liraglutide,IDegLira)对2型糖尿病(T2DM)合并颈动脉斑块患者的临床疗效。方法86例T2DM合并颈动脉斑块患者,随机分为对照组和观察组,每组43例。试验过程中退出3例,其中对照组41例完成试验,观察组42例完成试验。对照组给予德谷胰岛素、二甲双胍片联合他汀类药物治疗,同时给予饮食运动教育;观察组将德谷胰岛素替换为IDegLira,其余治疗不变。比较两组受试者治疗前后血脂、血糖、体重指数(BMI)、腰围以及颈动脉内膜中层厚度(IMT)、颈动脉斑块面积及胰岛素用量、不良反应发生情况。结果治疗后,两组IMT、颈动脉斑块面积均较基线改善,且干预组IMT(0.98±0.42)mm和颈动脉斑块面积(16.48±9.68)mm^(2)均小于对照组的(1.21±0.36)mm、(21.21±10.87)mm^(2)(P<0.05)。治疗后,两组空腹血糖(FPG)、餐后2小时血糖(2 h PG)、糖化血红蛋白(HbA1c)水平均低于本组治疗前,且观察组FPG(5.98±1.82)mmol/L、2 h PG(7.48±2.68)mmol/L、HbA1c(6.80±1.24)%均低于对照组的(7.21±1.56)mmol/L、(9.54±4.87)mmol/L、(8.55±2.27)%(P<0.05)。治疗后,两组总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平均低于本组治疗前,且观察组TC(3.92±0.89)mmol/L、LDL-C(2.80±1.24)mmol/L均低于对照组的(5.14±0.92)、(3.55±1.27)mmol/L(P<0.05);观察组甘油三酯水平低于本组治疗前(P<0.05)。治疗后,观察组腰围、BMI均低于本组治疗前,且观察组腰围(91.68±7.28)cm、BMI(23.26±1.36)kg/m^(2)均低于对照组的(95.24±8.23)cm、(25.74±2.48)kg/m^(2)(P<0.05);观察组胰岛素用量(18.67±5.26)U/d少于对照组的(22.65±6.76)U/d(P<0.05)。两组不良反应发生率比较无明显统计学差异(P>0.05)。结论IDegLira能显著改善T2DM合并颈动脉斑块患者的血管条件,缩小斑块面积,降低心脑血管发病风险,进一步改善患者血糖、血脂水平,同时能减重控制腰围,且不增加药物不良反应,可作为T2DM合并颈动脉斑块患者的优选。
