Tick studies in Malaysia have experienced a dynamic evolution characterized by periods of growth,stagnation,and the potential for revival.Beginning during the colonial era in the early 1900s,tick studies were primaril...Tick studies in Malaysia have experienced a dynamic evolution characterized by periods of growth,stagnation,and the potential for revival.Beginning during the colonial era in the early 1900s,tick studies were primarily conducted by European scientists and curators,establishing the foundation for tick taxonomy in the region.Pioneering works by George Henry Falknier Nuttall and Cecil Warburton introduced several new tick species,including Haemaphysalis(H.)calva,H.mjoebergi,H.vidua and H.wellingtoni[1].However,some records from this period are now considered doubtful,for instance Amblyomma(A.)breviscutatum,A.clypeolatum and A.integrum.The 1929 description of Ornithodoros batuensis by Stanley Hirst[2]marked the first documentation of a soft tick species in Malaysia,setting the stage for subsequent research endeavours.The Golden Age of tick studies(early 1950s-late 1980s)in Malaysia saw a surge in tick research activities.展开更多
Objective:To confirm the presence of Culex(Cx.)(Lophoceraomyia)cinctellus in Sri Lanka using morphological and molecular evidence.Methods:From October 2019 to April 2020,mosquito surveillance was conducted fort-nightl...Objective:To confirm the presence of Culex(Cx.)(Lophoceraomyia)cinctellus in Sri Lanka using morphological and molecular evidence.Methods:From October 2019 to April 2020,mosquito surveillance was conducted fort-nightly in the Banduragoda Public Health Inspector area.Larvae were collected using standard siphoning methods,while adults were sampled using Cattle Baited Trap,Gravid Traps,Light Traps,Bird-Baited Traps,Dog Baited Traps,and diurnal human landing collections.Specimens were transported to the Entomology Laboratory at the Medical Research Institute for identification.Morphological identification was performed using standard taxonomic keys.Molecular confirmation was achieved through DNA sequencing of mosquito head and thoracic regions,followed by sequence analysis using NCBI BLAST and Geneious software(version 7.1.3).Results:Adults of Cx.cinctellus were identified in Bird-Baited Traps and human bait collections.Unique morphological characteristics,including well-developed pulvilli,wing vein 1A ending before the apex of cross vein mcu,basal transverse pale bands on abdominal terga,and two labial basal setae on the proboscis,confirmed species identity.Morphometric measurements included mean thoracic length(0.58±0.02)mm,thoracic width(0.63±0.02)mm,abdominal length(2.15±0.03)mm,abdominal width(0.61±0.01)mm,and wing length(2.91±0.02)mm.Molecular analysis corroborated the morphological identification,affirming the species as Cx.cinctellus.COI sequences of the collected specimen(452 bp)were confirmed as Cx.cinctellus for sequence identity by BLAST and BOLD analysis.These sequences were subsequently deposited in GenBank under the accession number OR225623.1.Conclusions:This study documents the first occurrence of Cx.cinctellus in Sri Lanka,highlighting the need to enhance entomological surveillance to monitor its dispersal and population dynamics.展开更多
该文报道了2017年中国-缅甸边境云南省的泸水、福贡、贡山县采获的吸血蠓2属16种,包括库蠓属15种,蠛蠓属1种;新发现库蠓属库蠓亚属1新种:泸水库蠓[Culicoides(C.)lushuiensis Liu et Feng,sp.nov.];云南省首次纪录3种,即多孔库蠓、新替...该文报道了2017年中国-缅甸边境云南省的泸水、福贡、贡山县采获的吸血蠓2属16种,包括库蠓属15种,蠛蠓属1种;新发现库蠓属库蠓亚属1新种:泸水库蠓[Culicoides(C.)lushuiensis Liu et Feng,sp.nov.];云南省首次纪录3种,即多孔库蠓、新替库蠓、海南蠛蠓。泸水库蠓与杜比库蠓(C. dubius Arnaud,1956)相近似,但后者雌虫翅臀室近端部的淡斑卵圆形,触须比为3.00;雄虫第9腹板后缘凹陷呈宽"V"形,第9背板后缘微突,抱器基节腹踝矩刺状,阳茎中叶端部短而粗,阳茎拱高约为阳茎中叶总长的1/2与本新种明显不相同。新种模式标本保存在沈阳军区CDC(沈阳110034)。展开更多
基金supported by the Higher Institution Centre of Excellence(HICoE)program(MO002-2019&TIDREC-2023)funded by the Bernhard Nocht Institute for Tropical Medicine,Hamburg,Germany[100-TNCPI/INT 16/6/2(005/2020)].
文摘Tick studies in Malaysia have experienced a dynamic evolution characterized by periods of growth,stagnation,and the potential for revival.Beginning during the colonial era in the early 1900s,tick studies were primarily conducted by European scientists and curators,establishing the foundation for tick taxonomy in the region.Pioneering works by George Henry Falknier Nuttall and Cecil Warburton introduced several new tick species,including Haemaphysalis(H.)calva,H.mjoebergi,H.vidua and H.wellingtoni[1].However,some records from this period are now considered doubtful,for instance Amblyomma(A.)breviscutatum,A.clypeolatum and A.integrum.The 1929 description of Ornithodoros batuensis by Stanley Hirst[2]marked the first documentation of a soft tick species in Malaysia,setting the stage for subsequent research endeavours.The Golden Age of tick studies(early 1950s-late 1980s)in Malaysia saw a surge in tick research activities.
文摘Objective:To confirm the presence of Culex(Cx.)(Lophoceraomyia)cinctellus in Sri Lanka using morphological and molecular evidence.Methods:From October 2019 to April 2020,mosquito surveillance was conducted fort-nightly in the Banduragoda Public Health Inspector area.Larvae were collected using standard siphoning methods,while adults were sampled using Cattle Baited Trap,Gravid Traps,Light Traps,Bird-Baited Traps,Dog Baited Traps,and diurnal human landing collections.Specimens were transported to the Entomology Laboratory at the Medical Research Institute for identification.Morphological identification was performed using standard taxonomic keys.Molecular confirmation was achieved through DNA sequencing of mosquito head and thoracic regions,followed by sequence analysis using NCBI BLAST and Geneious software(version 7.1.3).Results:Adults of Cx.cinctellus were identified in Bird-Baited Traps and human bait collections.Unique morphological characteristics,including well-developed pulvilli,wing vein 1A ending before the apex of cross vein mcu,basal transverse pale bands on abdominal terga,and two labial basal setae on the proboscis,confirmed species identity.Morphometric measurements included mean thoracic length(0.58±0.02)mm,thoracic width(0.63±0.02)mm,abdominal length(2.15±0.03)mm,abdominal width(0.61±0.01)mm,and wing length(2.91±0.02)mm.Molecular analysis corroborated the morphological identification,affirming the species as Cx.cinctellus.COI sequences of the collected specimen(452 bp)were confirmed as Cx.cinctellus for sequence identity by BLAST and BOLD analysis.These sequences were subsequently deposited in GenBank under the accession number OR225623.1.Conclusions:This study documents the first occurrence of Cx.cinctellus in Sri Lanka,highlighting the need to enhance entomological surveillance to monitor its dispersal and population dynamics.
文摘该文报道了2017年中国-缅甸边境云南省的泸水、福贡、贡山县采获的吸血蠓2属16种,包括库蠓属15种,蠛蠓属1种;新发现库蠓属库蠓亚属1新种:泸水库蠓[Culicoides(C.)lushuiensis Liu et Feng,sp.nov.];云南省首次纪录3种,即多孔库蠓、新替库蠓、海南蠛蠓。泸水库蠓与杜比库蠓(C. dubius Arnaud,1956)相近似,但后者雌虫翅臀室近端部的淡斑卵圆形,触须比为3.00;雄虫第9腹板后缘凹陷呈宽"V"形,第9背板后缘微突,抱器基节腹踝矩刺状,阳茎中叶端部短而粗,阳茎拱高约为阳茎中叶总长的1/2与本新种明显不相同。新种模式标本保存在沈阳军区CDC(沈阳110034)。
