Mosquito-borne diseases pose a significant global health threat,necessitating the development of innovative vector control strategies.In this study,we investigated the potential of harnessing host immunity against mos...Mosquito-borne diseases pose a significant global health threat,necessitating the development of innovative vector control strategies.In this study,we investigated the potential of harnessing host immunity against mosquitoes through vaccination.Using Culex pipiens(C.pipiens)as a model,we demonstrated that polyclonal antibodies against C.pipiens abdominal protein extracts significantly impaired oviposition and increased mosquito mortality,primarily through the classical complement activation pathways.However,repeated exposure led to resistance,indicating potential adaptation.Proteomic analysis identified metabolic proteins as key targets,with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighting their roles in carboxylic acid metabolism,tyrosine degradation,and the proteasome pathways.Notably,cross-species reactivity was revealed by Western blotting,showing strong binding of Culex-specific antibodies to Anopheles and Aedes abdominal proteins.This study provides mechanistic insights into antibody-based mosquito suppression,highlighting its potential as an innovative vector control strategy while underscoring the need for further research on resistance management and ecological impacts.展开更多
Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)b...Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)by bioinformatics prediction and dual-luciferase reporter assays.Following intrathoracic injection of miR-11903a agomir and antagomir,Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a.Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae,followed by pupae and adults,and highly expressed in the wings,head,and midgut of female adults.Following pathogen infection,AeCLIPB9 and miR-11903a exhibited opposite expression trends,indicating their potential roles in mosquito innate immunity.To further investigate the relationship between AeCLIPB9 and miR-11903a,double-strand CLIPB9 was synthesized and RNA interference was performed.Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens.Finally,longevity assays demonstrated that miR-11903a influenced mosquito lifespan.展开更多
Aedes albopictus(Ae.albopictus)is widely distributed and can transmit many infectious diseases,and insecticide-based interventions play an important role in vector control.However,increased insecticide resistance has ...Aedes albopictus(Ae.albopictus)is widely distributed and can transmit many infectious diseases,and insecticide-based interventions play an important role in vector control.However,increased insecticide resistance has become a severe public health problem,and the clarification of its detailed mechanism is a matter of urgence.This study found that target-site resistance and metabolic resistance could not fully explain insecticide resistance in field Ae.albopictus,and there were likely other resistance mechanisms involved.The 16S and internal transcribed spacer sequencing revealed significant differences in the species compositions of the cuticle surface symbiotic bacteria and fungi between deltamethrin(DM)-resistant(DR)and DM-susceptible(DS)Ae.albopictus.Additionally,the abundances of Serratia spp.and Candida spp.significantly increased after DM treatment.Furthermore,2 fungi(Rhodotorula mucilaginosa and Candida melibiosica)and 3 bacteria(Serratia marcescens,Klebsiella aerogenes,and Serratia sp.)isolated from DR Ae.albopictus can use DM as their sole carbon source.After reinoculation onto the cuticle surface of DS Ae.albopictus,R.mucilaginosa and C.melibiosica significantly enhanced the DM resistance of Ae.albopictus.Moreover,transcriptome sequencing of the surviving Ae.albopictus after DM exposure revealed that the gene expression of cytochrome P450 enzymes and glutathione-S-transferases increased,suggesting that besides the direct degradation,the candidate degrading microbes could also cause insecticide resistance via indirect enhancement of mosquito gene expression.In conclusion,we demonstrated that the cuticle surface symbiotic microbes were involved in the development of insecticide resistance in Ae.albopictus,providing novel and supplementary insights into insecticide resistance mechanisms.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.82472312).
文摘Mosquito-borne diseases pose a significant global health threat,necessitating the development of innovative vector control strategies.In this study,we investigated the potential of harnessing host immunity against mosquitoes through vaccination.Using Culex pipiens(C.pipiens)as a model,we demonstrated that polyclonal antibodies against C.pipiens abdominal protein extracts significantly impaired oviposition and increased mosquito mortality,primarily through the classical complement activation pathways.However,repeated exposure led to resistance,indicating potential adaptation.Proteomic analysis identified metabolic proteins as key targets,with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighting their roles in carboxylic acid metabolism,tyrosine degradation,and the proteasome pathways.Notably,cross-species reactivity was revealed by Western blotting,showing strong binding of Culex-specific antibodies to Anopheles and Aedes abdominal proteins.This study provides mechanistic insights into antibody-based mosquito suppression,highlighting its potential as an innovative vector control strategy while underscoring the need for further research on resistance management and ecological impacts.
基金supported by the Major Science and Technology Plan of Hainan Province(ZDKJ2021035)the National Natural Science Foundation of China(U22A20363)the Innovative Research Projects for Postgraduates in Hainan Province(Qhyb2022-41).
文摘Arthropod melanization is a crucial defense mechanism mediated by a complex cascade of CLIP domain serine proteases(CLIPs).In this study,it was confirmed that microRNA-11903a(miR-11903a)targets Aedes-CLIPB9(AeCLIPB9)by bioinformatics prediction and dual-luciferase reporter assays.Following intrathoracic injection of miR-11903a agomir and antagomir,Real-time quantitative polymerase chain reaction confirmed that AeCLIPB9 is negatively regulated by miR-11903a.Spatiotemporal expression analysis revealed that miR-11903a is most abundant in 4th instar larvae,followed by pupae and adults,and highly expressed in the wings,head,and midgut of female adults.Following pathogen infection,AeCLIPB9 and miR-11903a exhibited opposite expression trends,indicating their potential roles in mosquito innate immunity.To further investigate the relationship between AeCLIPB9 and miR-11903a,double-strand CLIPB9 was synthesized and RNA interference was performed.Seven-d survival assays revealed that both AeCLIPB9 and miR-11903a were crucial immune factors in fighting pathogens.Finally,longevity assays demonstrated that miR-11903a influenced mosquito lifespan.
基金supported by the Key Research and Development Program sponsored by the Ministry of Science and Technology(MOST)(2023YFA1801004)National Natural Science Foundation of China and the Bill and Melinda Gates Foundation joint program(82261128002,2022YFML1003).
文摘Aedes albopictus(Ae.albopictus)is widely distributed and can transmit many infectious diseases,and insecticide-based interventions play an important role in vector control.However,increased insecticide resistance has become a severe public health problem,and the clarification of its detailed mechanism is a matter of urgence.This study found that target-site resistance and metabolic resistance could not fully explain insecticide resistance in field Ae.albopictus,and there were likely other resistance mechanisms involved.The 16S and internal transcribed spacer sequencing revealed significant differences in the species compositions of the cuticle surface symbiotic bacteria and fungi between deltamethrin(DM)-resistant(DR)and DM-susceptible(DS)Ae.albopictus.Additionally,the abundances of Serratia spp.and Candida spp.significantly increased after DM treatment.Furthermore,2 fungi(Rhodotorula mucilaginosa and Candida melibiosica)and 3 bacteria(Serratia marcescens,Klebsiella aerogenes,and Serratia sp.)isolated from DR Ae.albopictus can use DM as their sole carbon source.After reinoculation onto the cuticle surface of DS Ae.albopictus,R.mucilaginosa and C.melibiosica significantly enhanced the DM resistance of Ae.albopictus.Moreover,transcriptome sequencing of the surviving Ae.albopictus after DM exposure revealed that the gene expression of cytochrome P450 enzymes and glutathione-S-transferases increased,suggesting that besides the direct degradation,the candidate degrading microbes could also cause insecticide resistance via indirect enhancement of mosquito gene expression.In conclusion,we demonstrated that the cuticle surface symbiotic microbes were involved in the development of insecticide resistance in Ae.albopictus,providing novel and supplementary insights into insecticide resistance mechanisms.
