Objective: To study the mechanism of anti-endotoxemic effect of Reduqing Injection (RDQ)and to explore the essence of traditional Chinese 'heat-clearing and detoxifying therapy'. Methods: A disseminated intrav...Objective: To study the mechanism of anti-endotoxemic effect of Reduqing Injection (RDQ)and to explore the essence of traditional Chinese 'heat-clearing and detoxifying therapy'. Methods: A disseminated intravascular coagulation (DIC) model was made in rabbits by intravenous injection with E. colt endotoxin. Increased plasma levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) and platelet activating factor (PAF), as well as the ex-vivo LPS-induced interleukin-1 (IL-1 ) production in peripheral blood monocytes were observed in model (M) group. Results: Same parameters were significantly lower in RDQ and dexamethasone (DXM) groups than those in the M group (P < 0. 01, P < 0. 05). Histopathological examination showed severe damage of the liver, lungs and kidneys in the rabbits of M group, in contrast, only mild affects were seen in the RDQ and DXM groups. Conclusions: RDQ exhibits protective effect on rabbits against endotoxin-induced DIC. The suppression of cytokines and inflammatory mediator PAF by RDQ may play a central role in the inhibition of endotoxin-induced DIC cascade.展开更多
文摘Objective: To study the mechanism of anti-endotoxemic effect of Reduqing Injection (RDQ)and to explore the essence of traditional Chinese 'heat-clearing and detoxifying therapy'. Methods: A disseminated intravascular coagulation (DIC) model was made in rabbits by intravenous injection with E. colt endotoxin. Increased plasma levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) and platelet activating factor (PAF), as well as the ex-vivo LPS-induced interleukin-1 (IL-1 ) production in peripheral blood monocytes were observed in model (M) group. Results: Same parameters were significantly lower in RDQ and dexamethasone (DXM) groups than those in the M group (P < 0. 01, P < 0. 05). Histopathological examination showed severe damage of the liver, lungs and kidneys in the rabbits of M group, in contrast, only mild affects were seen in the RDQ and DXM groups. Conclusions: RDQ exhibits protective effect on rabbits against endotoxin-induced DIC. The suppression of cytokines and inflammatory mediator PAF by RDQ may play a central role in the inhibition of endotoxin-induced DIC cascade.
