The phylogenetic relationships of the avian genus Crossoptilon were studied by comparing mitochondrial control region(D-loop)and cyt b gene nucleotide sequences,with the Lophophorus impejanus and Lophura swinhoii as t...The phylogenetic relationships of the avian genus Crossoptilon were studied by comparing mitochondrial control region(D-loop)and cyt b gene nucleotide sequences,with the Lophophorus impejanus and Lophura swinhoii as the outgroups.Using the synthesizing characters of morphology,ecology and biogeography to combine with Maximum parsimony(MP),Maximum Likelihood(ML)and Bayesin trees,we found that:① C.crossoptilon and C.harmani were closely related and C.harmani was relatively primitive;② C.auritum and C.mantchuricum had a close relationship and C.auritum was relatively primitive;③ the genus Crossoptilon could originate in the boundary region of Sichuan,Yunnan and Tibet.One branch remained near the center of origin and diverged into C.crossoptilon and C.harmani;the other branch spread towards north China and diverged into C.auritum and C.mantchuricum.展开更多
为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish...为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish）、水泡眼（Water vesicle goldfish）、珍珠（Genuine pearl goldfish）的线粒体DNA上细胞色素b的部分核苷酸序列，长度为597bp。结合GenBank中红鲫、野鲫、日本白鲫、银鲫、鲤鱼的序列进行比较分析，结果显示，这7个金鱼品种之间的同源性都很高，在99．5％-100％之间；7种金鱼和红鲫的同源性也很高，为99．5％～99．8％，与野鲫的同源性在96．8％～97．2％，与日本白鲫、银鲫的同源性为93．1％～94．3％，与鲤鱼的同源性相对较低，为88．3％-88．6％。利用DNAstar软件构建了不同品种金鱼和鲫鱼的分子系统树，从分子水平进一步证实了金鱼起源于野鲫。展开更多
文摘The phylogenetic relationships of the avian genus Crossoptilon were studied by comparing mitochondrial control region(D-loop)and cyt b gene nucleotide sequences,with the Lophophorus impejanus and Lophura swinhoii as the outgroups.Using the synthesizing characters of morphology,ecology and biogeography to combine with Maximum parsimony(MP),Maximum Likelihood(ML)and Bayesin trees,we found that:① C.crossoptilon and C.harmani were closely related and C.harmani was relatively primitive;② C.auritum and C.mantchuricum had a close relationship and C.auritum was relatively primitive;③ the genus Crossoptilon could originate in the boundary region of Sichuan,Yunnan and Tibet.One branch remained near the center of origin and diverged into C.crossoptilon and C.harmani;the other branch spread towards north China and diverged into C.auritum and C.mantchuricum.
文摘为探讨不同品种金鱼的系统进化关系，利用PCR技术扩增了金鱼的7个代表品种红龙睛（Red dragon goldfish）、红帽子（Red cap goldfish）、虎头（Tiger head goldfish）、琉金（Gold plating goldfish）、墨龙睛（Black dragon goldfish）、水泡眼（Water vesicle goldfish）、珍珠（Genuine pearl goldfish）的线粒体DNA上细胞色素b的部分核苷酸序列，长度为597bp。结合GenBank中红鲫、野鲫、日本白鲫、银鲫、鲤鱼的序列进行比较分析，结果显示，这7个金鱼品种之间的同源性都很高，在99．5％-100％之间；7种金鱼和红鲫的同源性也很高，为99．5％～99．8％，与野鲫的同源性在96．8％～97．2％，与日本白鲫、银鲫的同源性为93．1％～94．3％，与鲤鱼的同源性相对较低，为88．3％-88．6％。利用DNAstar软件构建了不同品种金鱼和鲫鱼的分子系统树，从分子水平进一步证实了金鱼起源于野鲫。
