摘要
从鲷科鱼类中黄鳍鲷(Sparus latus)、黑鲷(S.macrocephalus)、真鲷(Pagrosomus major)和平鲷(Rhabdosargussarba)的线粒体DNA扩增出约430 bp的细胞色素b(Cytb)基因,经Clastal X同源排序后得408 bp序列,对该序列进行分析并结合GeneBank中黄鲷(Taius tumifrons)、灰鳍鲷(S.berda)、犁齿鲷(Evynnis japonica)和高背四长棘鲷(Argyrops spinifer)的该区段DNA序列比较,共发现126个核苷酸位点存在变异(30.7％),序列中的转换大于颠换,碱基替换多发生在密码子的第3位点。黑鲷和灰鳍鲷亲缘关系最近,序列差异为3.8％;而黄鲷和平鲷的亲缘关系最远,序列差异为21.7％。用MEGA2.1软件中的NJ法构建的分子系统树,将上述8种类分为4组,其中黄鲷属、平鲷属和鲷属分类的结果与形态分类学的观点一致;而传统分类上把犁齿鲷、真鲷和高背四长棘鲷归为3个不同属,在本研究分子系统树中,这3个属却聚成一支。本研究目的在于从分子水平上阐明鲷科鱼类的分类与系统进化关系。
The 430 bp fragments of Cytochrome b gene were obtained by PCR method from the mitochondrial DNA of Sparus latus, Spams macrocephalus, Pagrosomus major and Rhabdosargus sarba, and a mitochondrial Cyt b DNA of 408 bp sequence was successfully sequenced by ClastalX homologous sequence analysis. By comparing with the same DNA fragments of Sparus berda, Taius tumifrons, Evynnis japonica and Argyrops spinifer recorded in the Gene-Bank, the sequences of 408 bp DNA have 126 nucleotide sites substituted (30.7% ), and the transition times are more than the trasversion times; most of the base substitution happens in the third locus of the codon. The closest relationship is between 5. macrocephalus and 5. berda, and T. tumifrons is highly divergent from R. sarba. The molecular phylogenetic tree constructed by NJ method of MAGE2. 1 indicates the eight fishes are divided into four groups, in which R. sarba, T. tumifrons and the species of Sparus are divided into three branches, respectively, which in consistent with the conclusion of morphological analysis. But P. major, E. japonica and A. spinifer are clustered together, which is different from the result of traditional taxonomy which divided the three species into three different genera. The purpose of this study is to clarify the taxonomy and phylogenetic evolution relationship of Sparidae fishes from the view of molecular biology.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第3期184-188,共5页
Journal of Fishery Sciences of China
基金
国家基础性工作专项
广东省科技兴海重大项目(A200099A01)
中国水产科学研究院人才基金
