城市化进程中的土地开发、水利工程建设等活动对淡水生态系统造成了不可逆转的损害。这些行为严重破坏了淡水生态系统的自然结构和功能,水质污染问题日益严重,生物栖息地逐渐丧失,这对淡水生物多样性和生态平衡构成了巨大威胁。识别导...城市化进程中的土地开发、水利工程建设等活动对淡水生态系统造成了不可逆转的损害。这些行为严重破坏了淡水生态系统的自然结构和功能,水质污染问题日益严重,生物栖息地逐渐丧失,这对淡水生物多样性和生态平衡构成了巨大威胁。识别导致水生生物多样性下降的压力源对浑太河流域生态保护与恢复、水资源管理等方面都具有重要意义。本研究通过对浑太河277个采样点的监测,探究不同区域环境压力源对水生生物群落和多样性之间的影响。结果显示:①浑太河采集到着生藻454种、大型底栖动物140种和鱼类47种;②结构方程模型显示,水质指数(waterquality in dex,WQI)对多营养级生物群落α多样性和β多样性的直接影响大于间接影响,且与α多样性显著正相关。此外,WQI通过多营养级生物物种丰度间接对多营养级生物群落α和β多样性产生影响;③从上游到下游随着WQI的增大,多营养级生物群落β多样性在整体上呈增大的趋势。该研究结果可为浑太河流域水生生物群落的保护和管理提供理论依据。展开更多
Coccolithophores have unique ability of dual carbon fixation(photosynthesis and biocalcification)and can accumulate bioactive compounds effectively,thereby showing huge potential for CO_(2) bioremediation and simultan...Coccolithophores have unique ability of dual carbon fixation(photosynthesis and biocalcification)and can accumulate bioactive compounds effectively,thereby showing huge potential for CO_(2) bioremediation and simultaneous production of valueadded substances.However,biotechnological exploration of these microalgae is still very limited.In order to develop efficient cultivation strategies of these organisms,we tested the abilities of Chrysotila roscoffensis,a coccolithophore,to grow under mixotrophic and heterotrophic cultivation conditions.It was confirmed that only glycerol stimulated the cell growth of this microalga in the presence of light.To study the physiological significance of mixotrophy,we conducted cell culture experiments both autotrophically and mixotrophically,the only difference of which was optimal amount of glycerol was added under mixotrophic conditions.The results showed that the addition of glycerol substantially enhanced the lipids and monounsaturated fatty acids accumulation of C.roscoffensis,but imposed slight influences on the photosynthetic capacity,pigments content,polyunsaturated fatty acids content,and biocalcification.We concluded that mixotrophy might be an appropriate strategy for exploration of this species for production of high value molecules.展开更多
The genus Selenastrum comprises common coccoid green algae found in diverse habitats worldwide.This genus has a complex taxonomic history,but recent applications of molecular phylogenetic methods have removed some mem...The genus Selenastrum comprises common coccoid green algae found in diverse habitats worldwide.This genus has a complex taxonomic history,but recent applications of molecular phylogenetic methods have removed some members with similar morphologies from Selenastrum and established new genera.However,due to the lack of available molecular sequences and isolates,the species diversity of the genus has not been fully explored.We conducted a detailed examination of the taxonomy of genus Selenastrum based on 11 new strains collected from China.The multi-disciplinary study utilized morphology,ultrastructure,and phylogeny based on multiple molecular markers,and ITS-2 secondary structure.All the included strains of genus Selenastrum were clustered into two distinct clades.The members of one clade were similar morphologically to the type species Selenastrum bibraianum,whereas the strains of the other clade had distinctly different colony structures.Based on the latter clade’s larger colony cell numbers and independent phylogenetic position,we proposed that it as a new species of the genus Selenastrum,namely Selenastrum densum sp.nov.Additional taxa sampling and molecular data will help to discover additional new species and clarify the validity of morphological taxonomic characteristics within the genus Selenastrum.展开更多
The euglenatides are a family of hybrid polyketide-nonribosomal peptides produced by the unicellular algae Euglena gracilis.These compounds have antiproliferative activity against fungal pathogensand mammalian cancer ...The euglenatides are a family of hybrid polyketide-nonribosomal peptides produced by the unicellular algae Euglena gracilis.These compounds have antiproliferative activity against fungal pathogensand mammalian cancer cell lines.Analysis of E.gracilis extracts revealed that the algae produce not only the euglenatides,but also a corresponding family of analogs that have the same molecular weights as the euglenatides,but are lacking the characteristic triene chromophore.In comparison to the euglenatides,the activity of these analogs is greatly reduced in a mammalian cytotoxicity assay,indicating that the triene is critical to the biological activity of the euglenatides.展开更多
Locations around the equator provide unique habitats for microalgae presumably with exceptional vitality.To develop microalga-derived product systems,we previously created a(sub)tropical microalgal collection.In this ...Locations around the equator provide unique habitats for microalgae presumably with exceptional vitality.To develop microalga-derived product systems,we previously created a(sub)tropical microalgal collection.In this study,two Chlorella strains(MEM176 and MEM193),adaptive to typical tropical climate,were isolated from the collection.The production performance was evaluated where both strains showed a robust growth in high temperatures and light intensities.Specifically,the strains MEM176 and MEM193 produced 503.6-mg/(L·d)and 411.3-mg/(L·d)biomass,respectively,with high contents of proteins.Their commercial and nutritional values were emphasized by amino acid compositions(e.g.,proline,valine,and phenylalanine).Particularly,higher amounts of proline were revealed in MEM176(47.9 mg/g dry biomass(DW))and MEM193(47.9 and 59.2 mg/g DW)than available commercial strains.Compared with MEM193,MEM176 produced 129.7%more lipids in which unsaturated fatty acids(particularly linoleic acid andα-linolenic acid)account for 65%of the total lipids.Therefore,it is promising to explore the potential of these Chlorella strains as food additives via outdoor cultivation in tropical area,notably MEM176 that exhibits superiority as sources of essential amino acids and valuable fatty acids.展开更多
Microalgae's adaptability and resilience to Earth's diverse environments have evolved these photosynthetic microor-ganisms into a biotechnological source of industrially relevant physiological functions and bi...Microalgae's adaptability and resilience to Earth's diverse environments have evolved these photosynthetic microor-ganisms into a biotechnological source of industrially relevant physiological functions and biometabolites.Despite this,microalgae-based industries only exploit a handful of species.This lack of biodiversity hinders the expansion of the microalgal industry.Microalgal bioprospecting,searching for novel biological algal resources with new properties,remains a low throughput and time-consuming endeavour due to inefficient workflows that rely on non-selective sampling,monoalgal culture status and outdated,non-standardized characterization techniques.This review will highlight the importance of microalgal bioprospecting and critically explore commonly employed methodologies.We will also explore current advances driving the next generation of smart algal bioprospecting focusing on novel workflows and transdisciplinary methodologies with the potential to enable high-throughput microalgal biodiscoveries.Images adapted from(Addicted04 in Wikipedia File:Australia on the globe(Australia centered).svg.2014.;Jin et al.in ACS Appl Bio Mater 4:5080-5089,2021;Kim et al.in Microchim Acta 189:88,2022;Tony et al.in Lab on a Chip 15,19:3810-3810;Thermo Fisher Scientific INC.in CTS Rotea Brochure).展开更多
文摘城市化进程中的土地开发、水利工程建设等活动对淡水生态系统造成了不可逆转的损害。这些行为严重破坏了淡水生态系统的自然结构和功能,水质污染问题日益严重,生物栖息地逐渐丧失,这对淡水生物多样性和生态平衡构成了巨大威胁。识别导致水生生物多样性下降的压力源对浑太河流域生态保护与恢复、水资源管理等方面都具有重要意义。本研究通过对浑太河277个采样点的监测,探究不同区域环境压力源对水生生物群落和多样性之间的影响。结果显示:①浑太河采集到着生藻454种、大型底栖动物140种和鱼类47种;②结构方程模型显示,水质指数(waterquality in dex,WQI)对多营养级生物群落α多样性和β多样性的直接影响大于间接影响,且与α多样性显著正相关。此外,WQI通过多营养级生物物种丰度间接对多营养级生物群落α和β多样性产生影响;③从上游到下游随着WQI的增大,多营养级生物群落β多样性在整体上呈增大的趋势。该研究结果可为浑太河流域水生生物群落的保护和管理提供理论依据。
基金supported by the Ningbo Science and Technology Project(No.2024J176)the Key Program of Science and Technology Innovation in Ningbo(No.2023 Z118)the Zhejiang Provincial Natural Science Foundation of China(No.LY22C190001)。
文摘Coccolithophores have unique ability of dual carbon fixation(photosynthesis and biocalcification)and can accumulate bioactive compounds effectively,thereby showing huge potential for CO_(2) bioremediation and simultaneous production of valueadded substances.However,biotechnological exploration of these microalgae is still very limited.In order to develop efficient cultivation strategies of these organisms,we tested the abilities of Chrysotila roscoffensis,a coccolithophore,to grow under mixotrophic and heterotrophic cultivation conditions.It was confirmed that only glycerol stimulated the cell growth of this microalga in the presence of light.To study the physiological significance of mixotrophy,we conducted cell culture experiments both autotrophically and mixotrophically,the only difference of which was optimal amount of glycerol was added under mixotrophic conditions.The results showed that the addition of glycerol substantially enhanced the lipids and monounsaturated fatty acids accumulation of C.roscoffensis,but imposed slight influences on the photosynthetic capacity,pigments content,polyunsaturated fatty acids content,and biocalcification.We concluded that mixotrophy might be an appropriate strategy for exploration of this species for production of high value molecules.
基金Supported by the National Natural Science Foundation of China(Nos.32370219,32270220,U22A20445)the Fundamental Research Program of Shanxi Province(No.202303021221082)+2 种基金the Open Project Program of Xinghuacun College of Shanxi University(Shanxi Institute of Brewing Technology and Industry)(No.XCSXU-KF-202320)the Yuncheng Salt Lake Protection and Utilization Research Institute of Shanxi,China(No.YHYJ-2023003)the Program for Young Scholar Talents of Wenying in Shanxi University。
文摘The genus Selenastrum comprises common coccoid green algae found in diverse habitats worldwide.This genus has a complex taxonomic history,but recent applications of molecular phylogenetic methods have removed some members with similar morphologies from Selenastrum and established new genera.However,due to the lack of available molecular sequences and isolates,the species diversity of the genus has not been fully explored.We conducted a detailed examination of the taxonomy of genus Selenastrum based on 11 new strains collected from China.The multi-disciplinary study utilized morphology,ultrastructure,and phylogeny based on multiple molecular markers,and ITS-2 secondary structure.All the included strains of genus Selenastrum were clustered into two distinct clades.The members of one clade were similar morphologically to the type species Selenastrum bibraianum,whereas the strains of the other clade had distinctly different colony structures.Based on the latter clade’s larger colony cell numbers and independent phylogenetic position,we proposed that it as a new species of the genus Selenastrum,namely Selenastrum densum sp.nov.Additional taxa sampling and molecular data will help to discover additional new species and clarify the validity of morphological taxonomic characteristics within the genus Selenastrum.
基金supported by a grant from the National Institutes of Health(R35 GM144076 to R.A.B.).
文摘The euglenatides are a family of hybrid polyketide-nonribosomal peptides produced by the unicellular algae Euglena gracilis.These compounds have antiproliferative activity against fungal pathogensand mammalian cancer cell lines.Analysis of E.gracilis extracts revealed that the algae produce not only the euglenatides,but also a corresponding family of analogs that have the same molecular weights as the euglenatides,but are lacking the characteristic triene chromophore.In comparison to the euglenatides,the activity of these analogs is greatly reduced in a mammalian cytotoxicity assay,indicating that the triene is critical to the biological activity of the euglenatides.
基金Supported by the National Key R&D Program of China(Nos.2021YFA0909600,2021YFE0110100)the National Natural Science Foundation of China(Nos.32060061,32370380)+3 种基金the Key R&D Program of Hainan Province(Nos.ZDYF2024XDNY244,ZDYF2022XDNY140)the Natural Science Foundation of Hainan Province(No.322QN250)the Foreign Expert Foundation of Hainan Province(No.G20230607016E)the Program of Key Lab of Ministry of Education for Utilization and Conservation of Tropical Marine Bioresources(No.2023SCNFKF04)。
文摘Locations around the equator provide unique habitats for microalgae presumably with exceptional vitality.To develop microalga-derived product systems,we previously created a(sub)tropical microalgal collection.In this study,two Chlorella strains(MEM176 and MEM193),adaptive to typical tropical climate,were isolated from the collection.The production performance was evaluated where both strains showed a robust growth in high temperatures and light intensities.Specifically,the strains MEM176 and MEM193 produced 503.6-mg/(L·d)and 411.3-mg/(L·d)biomass,respectively,with high contents of proteins.Their commercial and nutritional values were emphasized by amino acid compositions(e.g.,proline,valine,and phenylalanine).Particularly,higher amounts of proline were revealed in MEM176(47.9 mg/g dry biomass(DW))and MEM193(47.9 and 59.2 mg/g DW)than available commercial strains.Compared with MEM193,MEM176 produced 129.7%more lipids in which unsaturated fatty acids(particularly linoleic acid andα-linolenic acid)account for 65%of the total lipids.Therefore,it is promising to explore the potential of these Chlorella strains as food additives via outdoor cultivation in tropical area,notably MEM176 that exhibits superiority as sources of essential amino acids and valuable fatty acids.
文摘Microalgae's adaptability and resilience to Earth's diverse environments have evolved these photosynthetic microor-ganisms into a biotechnological source of industrially relevant physiological functions and biometabolites.Despite this,microalgae-based industries only exploit a handful of species.This lack of biodiversity hinders the expansion of the microalgal industry.Microalgal bioprospecting,searching for novel biological algal resources with new properties,remains a low throughput and time-consuming endeavour due to inefficient workflows that rely on non-selective sampling,monoalgal culture status and outdated,non-standardized characterization techniques.This review will highlight the importance of microalgal bioprospecting and critically explore commonly employed methodologies.We will also explore current advances driving the next generation of smart algal bioprospecting focusing on novel workflows and transdisciplinary methodologies with the potential to enable high-throughput microalgal biodiscoveries.Images adapted from(Addicted04 in Wikipedia File:Australia on the globe(Australia centered).svg.2014.;Jin et al.in ACS Appl Bio Mater 4:5080-5089,2021;Kim et al.in Microchim Acta 189:88,2022;Tony et al.in Lab on a Chip 15,19:3810-3810;Thermo Fisher Scientific INC.in CTS Rotea Brochure).
