目的:探讨石斛合剂含药血清对高糖诱导的小鼠足细胞损伤的影响及作用机制。方法:体外培养小鼠肾小球足细胞(MPC5),筛选高糖造模浓度和时间及石斛合剂含药血清最佳给药浓度;将细胞分为正常组(5.5 mmol·L^(-1)葡萄糖+10%空白血清)、...目的:探讨石斛合剂含药血清对高糖诱导的小鼠足细胞损伤的影响及作用机制。方法:体外培养小鼠肾小球足细胞(MPC5),筛选高糖造模浓度和时间及石斛合剂含药血清最佳给药浓度;将细胞分为正常组(5.5 mmol·L^(-1)葡萄糖+10%空白血清)、模型组(30 mmol·L^(-1)葡萄糖+10%空白血清)、石斛合剂含药血清组(30 mmol·L^(-1)葡萄糖+10%石斛合剂含药血清),铁死亡抑制剂(Fer-1)组(30 mmol·L^(-1)葡萄糖+10%空白血清+1μmol·L^(-1)Fer-1)。采用试剂盒检测细胞中Fe^(2+)、乳酸脱氢酶(LDH)水平;酶联免疫吸附测定法(ELISA)检测细胞谷胱甘肽(GSH)、过氧化脂质(LPO)含量;荧光探针检测活性氧(ROS)水平;实时荧光定量聚合酶链式反应(Real-time PCR)检测足细胞中肾母细胞瘤基因-1(WT-1)、结蛋白(Desmin)、长链脂酰辅酶A合成酶4(ACSL4)和谷胱甘肽过氧化物酶4(GPX4)m RNA表达水平;蛋白免疫印迹法(Western blot)检测足细胞中WT-1、Desmin、ACSL4和GPX4蛋白表达水平。结果:与空白组比较,30 mmol·L^(-1)高糖干预48 h可显著降低足细胞活力(P<0.01),10%石斛合剂含药血清改善高糖诱导的足细胞活力最为显著(P<0.01)。与正常组比较,模型组足细胞内Fe^(2+)、LDH、LPO、ROS水平及Desmin、ACSL4 m RNA和蛋白表达显著升高(P<0.01),GSH水平及WT-1、GPX4 m RNA和蛋白表达显著降低(P<0.01);与模型组比较,石斛合剂含药血清组足细胞内Fe^(2+)、LDH、LPO、ROS水平及Desmin、ACSL4 m RNA和蛋白表达明显降低(P<0.05,P<0.01),GSH水平及WT-1、GPX4 m RNA和蛋白表达明显升高(P<0.05,P<0.01)。结论:石斛合剂含药血清对高糖诱导的足细胞损伤具有保护作用,与其抑制铁死亡有关。展开更多
Started from the liver targeting compounds（glycyrrhizin,glycyrrhetinicacid,bile acid）,six（amine-terminated） compounds were prepared via two-step reactions.The NMR experiments provided the direct evidence of the ex...Started from the liver targeting compounds（glycyrrhizin,glycyrrhetinicacid,bile acid）,six（amine-terminated） compounds were prepared via two-step reactions.The NMR experiments provided the direct evidence of the existence of amide.The polymers were synthesized by polymerizing of BLG-NCA initiated by each of six amine-terminated compounds.The liver targeting group was introduced to the main chain of the polymer.The molecular weight of the resultant polymers was adjusted by changing the molar ratio of monomer BLG-NCA to initiator.The product structure was characterized by GPC,FTIR and（）1H NMR.The results demonstrate that amine-terminated compounds containing liver targeting group could initiate the polymerization of BLG-NCA.展开更多
A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results show...A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results showed that the adsorbent modified by dimethylamine ligand with a long spacer had the best capacity for adsorbing endotoxin. The adsorbent P-N4,having a hydroxyl group at β -site of the ligand,could form the hydrogen bond with phosphate group. The electrostatic interaction,hydrogen bond and cooperative effect of octatomic ring worked simultaneously. All these factors are responsible for the strong interactions between endotoxin molecule and the adsorbent.展开更多
文摘目的:探讨石斛合剂含药血清对高糖诱导的小鼠足细胞损伤的影响及作用机制。方法:体外培养小鼠肾小球足细胞(MPC5),筛选高糖造模浓度和时间及石斛合剂含药血清最佳给药浓度;将细胞分为正常组(5.5 mmol·L^(-1)葡萄糖+10%空白血清)、模型组(30 mmol·L^(-1)葡萄糖+10%空白血清)、石斛合剂含药血清组(30 mmol·L^(-1)葡萄糖+10%石斛合剂含药血清),铁死亡抑制剂(Fer-1)组(30 mmol·L^(-1)葡萄糖+10%空白血清+1μmol·L^(-1)Fer-1)。采用试剂盒检测细胞中Fe^(2+)、乳酸脱氢酶(LDH)水平;酶联免疫吸附测定法(ELISA)检测细胞谷胱甘肽(GSH)、过氧化脂质(LPO)含量;荧光探针检测活性氧(ROS)水平;实时荧光定量聚合酶链式反应(Real-time PCR)检测足细胞中肾母细胞瘤基因-1(WT-1)、结蛋白(Desmin)、长链脂酰辅酶A合成酶4(ACSL4)和谷胱甘肽过氧化物酶4(GPX4)m RNA表达水平;蛋白免疫印迹法(Western blot)检测足细胞中WT-1、Desmin、ACSL4和GPX4蛋白表达水平。结果:与空白组比较,30 mmol·L^(-1)高糖干预48 h可显著降低足细胞活力(P<0.01),10%石斛合剂含药血清改善高糖诱导的足细胞活力最为显著(P<0.01)。与正常组比较,模型组足细胞内Fe^(2+)、LDH、LPO、ROS水平及Desmin、ACSL4 m RNA和蛋白表达显著升高(P<0.01),GSH水平及WT-1、GPX4 m RNA和蛋白表达显著降低(P<0.01);与模型组比较,石斛合剂含药血清组足细胞内Fe^(2+)、LDH、LPO、ROS水平及Desmin、ACSL4 m RNA和蛋白表达明显降低(P<0.05,P<0.01),GSH水平及WT-1、GPX4 m RNA和蛋白表达明显升高(P<0.05,P<0.01)。结论:石斛合剂含药血清对高糖诱导的足细胞损伤具有保护作用,与其抑制铁死亡有关。
文摘Started from the liver targeting compounds（glycyrrhizin,glycyrrhetinicacid,bile acid）,six（amine-terminated） compounds were prepared via two-step reactions.The NMR experiments provided the direct evidence of the existence of amide.The polymers were synthesized by polymerizing of BLG-NCA initiated by each of six amine-terminated compounds.The liver targeting group was introduced to the main chain of the polymer.The molecular weight of the resultant polymers was adjusted by changing the molar ratio of monomer BLG-NCA to initiator.The product structure was characterized by GPC,FTIR and（）1H NMR.The results demonstrate that amine-terminated compounds containing liver targeting group could initiate the polymerization of BLG-NCA.
文摘A series of modified poly(methyl methacrylate) adsorbents were prepared and their adsorption abilities to endotoxin,which is a component of the outer membrance of Gram-negative bacteria,were compared. The results showed that the adsorbent modified by dimethylamine ligand with a long spacer had the best capacity for adsorbing endotoxin. The adsorbent P-N4,having a hydroxyl group at β -site of the ligand,could form the hydrogen bond with phosphate group. The electrostatic interaction,hydrogen bond and cooperative effect of octatomic ring worked simultaneously. All these factors are responsible for the strong interactions between endotoxin molecule and the adsorbent.
