A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membran...A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membrane,the activity of cytochrome C oxidase and ATPase at subcellular level were investigated by using H\-2O\-2/Fe\+\{2+\} -induced mitochondria damage model system. It was found that the small peptide exhibited an obvious ability of removing free radicals and protecting the mitochondria from damage by oxidation. [WT5HZ]展开更多
文摘A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membrane,the activity of cytochrome C oxidase and ATPase at subcellular level were investigated by using H\-2O\-2/Fe\+\{2+\} -induced mitochondria damage model system. It was found that the small peptide exhibited an obvious ability of removing free radicals and protecting the mitochondria from damage by oxidation. [WT5HZ]
文摘为了对Se CPHs的抗氧化活性进行研究,通过老龄小鼠模型以及Hep G2细胞模型考察了普通玉米肽(CPs)、富硒玉米肽(Se CPs)以及甲基硒代半胱氨酸(Me Se Cys)的抗氧化活性。结果表明,采用Se CPs(400 mg/kg)连续灌胃30 d后,老龄小鼠血清中SOD、MDA、GSH-Px、T-AOC以及胸腺指数等指标均有显著改善,总抗氧化能力比CPs高13.38%,说明硒对抗氧化活性有重要贡献;当硒含量相等时,玉米肽比氨基酸的总抗氧化能力高15.79%。在Hep G2细胞模型中,高含硒量的Me Se Cys能极显著提高细胞增殖率。在改善细胞培养液中的ALT、AST和细胞中的SOD、GSH指标方面,Me Se Cys组(硒含量0.5μg/m L)和Se CPs+Me Se Cys组(硒含量0.25μg/m L)均有极显著效果,且水平相当,说明高含硒量化合物与玉米肽间具有协同抗氧化作用。
