In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine seru...In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine serum albumin (BSA) and calcium carbonate (CaCO3) in saline solution. The results demonstrated that there were strong interactions between BSA and calcium carbonate by hydrogen bonds or coordination or electrostatic attraction, which made the solubility of calcium carbonate in aqueous solution increased and the microstructure of BSA and CaCO3 changed. The interaction helped to form biomineral containing protein and CaCO3 in organism.展开更多
The interaction of Zincon(ZCN)-Cu(Ⅱ) metal complexe with bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution (pH=4.25). ZCN-Cu(Ⅱ)-BSA was a blueness color coordi...The interaction of Zincon(ZCN)-Cu(Ⅱ) metal complexe with bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution (pH=4.25). ZCN-Cu(Ⅱ)-BSA was a blueness color coordination compound, with maximum absorption at 605 nm with 10 nm, 60 nm and 140 nm of red shift compared to the ZCN-Cu(Ⅱ) complex, ZCN-BSA complex and ZCN. According to molar ratio method and dual wavelength method, the apparent molar absorptivity of ε was equal to 4.82×105 L·mol-1·cm-1. Conditional constants were defined as ZCN∶Cu(Ⅱ)∶BSA=10∶5∶2. Condition combination constant of ZCN-Cu(Ⅱ) and BSA K was 8.82×104. A combined mechanism was suggested between BSA and ZCN-Cu(Ⅱ) by electrostatic force (Primary action force) and distant water force. Position of Impact might be arginine, proline, histidine, tyrosine and cystine. Contemporary formation of electron transfer complexes is an important cause in forming ZCN-Cu(Ⅱ)-BSA complexes.展开更多
文摘In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine serum albumin (BSA) and calcium carbonate (CaCO3) in saline solution. The results demonstrated that there were strong interactions between BSA and calcium carbonate by hydrogen bonds or coordination or electrostatic attraction, which made the solubility of calcium carbonate in aqueous solution increased and the microstructure of BSA and CaCO3 changed. The interaction helped to form biomineral containing protein and CaCO3 in organism.
文摘The interaction of Zincon(ZCN)-Cu(Ⅱ) metal complexe with bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution (pH=4.25). ZCN-Cu(Ⅱ)-BSA was a blueness color coordination compound, with maximum absorption at 605 nm with 10 nm, 60 nm and 140 nm of red shift compared to the ZCN-Cu(Ⅱ) complex, ZCN-BSA complex and ZCN. According to molar ratio method and dual wavelength method, the apparent molar absorptivity of ε was equal to 4.82×105 L·mol-1·cm-1. Conditional constants were defined as ZCN∶Cu(Ⅱ)∶BSA=10∶5∶2. Condition combination constant of ZCN-Cu(Ⅱ) and BSA K was 8.82×104. A combined mechanism was suggested between BSA and ZCN-Cu(Ⅱ) by electrostatic force (Primary action force) and distant water force. Position of Impact might be arginine, proline, histidine, tyrosine and cystine. Contemporary formation of electron transfer complexes is an important cause in forming ZCN-Cu(Ⅱ)-BSA complexes.
