Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were ana...Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.展开更多
以绒毛皂荚(Gleditsia vestita Chun et How ex B.G.Li)种子为外植体,通过愈伤组织诱导、不定芽分化增殖、生根和驯化移栽,建立了绒毛皂荚组织培养的快速繁殖体系。结果表明:1)绒毛皂荚子叶和胚轴在MS+6-BA3.0 mg/L+KT 0.4 mg/L+IAA 0.3...以绒毛皂荚(Gleditsia vestita Chun et How ex B.G.Li)种子为外植体,通过愈伤组织诱导、不定芽分化增殖、生根和驯化移栽,建立了绒毛皂荚组织培养的快速繁殖体系。结果表明:1)绒毛皂荚子叶和胚轴在MS+6-BA3.0 mg/L+KT 0.4 mg/L+IAA 0.3 mg/L培养基上同步诱导出愈伤和芽,愈伤组织诱导率达到90.5%,同步分化率高达77.9%;2)不定芽增殖的最佳培养基为1/2MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+NAA 0.2 mg/L,培养20 d后,增殖倍数为4.0;3)MS+NAA 0.5 mg/L+IAA 0.25 mg/L条件下,生根率高达100%,且移栽后植株长势好,成活率达90%以上。展开更多
文摘Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.
