The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper....The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.展开更多
Methods for separating 1-(2,4-Dichlorophenyl) 4, 4-dimethyl 1-2(1,2,4-triazol-l-yl) 1-penten-3-ol isomers and D,L-tryptophan enantiomers using HPTLC and TLC were investigated. The effects on Separation made by differe...Methods for separating 1-(2,4-Dichlorophenyl) 4, 4-dimethyl 1-2(1,2,4-triazol-l-yl) 1-penten-3-ol isomers and D,L-tryptophan enantiomers using HPTLC and TLC were investigated. The effects on Separation made by different nonchiral mobile phase, chiral mobile phase and two different shapes of plates were studied. Useful separation was obtained forstereoisomers, and the resolution of D, L-tryptophan was good by using chiral mobile phase additive(D-quinia).展开更多
文摘The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-αA expressed in yeast, rhIFN-αA and rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-αA expressed in yeast as 70%, and rhIFN-αA expressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.
文摘Methods for separating 1-(2,4-Dichlorophenyl) 4, 4-dimethyl 1-2(1,2,4-triazol-l-yl) 1-penten-3-ol isomers and D,L-tryptophan enantiomers using HPTLC and TLC were investigated. The effects on Separation made by different nonchiral mobile phase, chiral mobile phase and two different shapes of plates were studied. Useful separation was obtained forstereoisomers, and the resolution of D, L-tryptophan was good by using chiral mobile phase additive(D-quinia).
