摘要
在７５μｍ（ｉ．ｄ．）×２７／２０ｃｍ的硅胶（３μｍ）填充柱上，以乙腈（９５：５）－Ｔｒｉｓ／ＨＣＩ缓冲液（ｐＨ８.３）为流动相，实施毛细管电色谱并对其保留机理进行了研究。研究表明，当采用反相洗脱时，硅胶填充柱显示出阳离子交换和正相分配双重保留机理。同时还对电渗流和热效应进行了研究。
The feasibility of capillary electrochromatography (CEC) was shown by demon- strating on 75 μm (i. d.)capillary columns packed with 3 μm silica packings using buffered aqueous acetonitrile mobile pbase.The retention mechanisms of this seperation system were discussed. The results indicated that the retention on silica column was determined by both canon ion exchange and normal obase retention mechanisms. The factors affected on electroosmotic flow in a silica packed columns were also investigated.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第3期287-290,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重点资助项目。
关键词
毛细管电色谱
硅胶填充柱
保留机理
CEC
Capillary electroobromatography,unmodified packed column, retention mechanism
