为提高泾阳茯茶蛋白降血脂肽(Jingyang fu tea protein hypolipidemic peptide,JFPHP)的稳定性,以明胶和阿拉伯胶作为壁材,JFPHP为芯材,采用复凝聚法制备JFPHP微胶囊.以包埋率为指标,在单因素实验基础上,采用响应面实验优化JFPHP微胶囊...为提高泾阳茯茶蛋白降血脂肽(Jingyang fu tea protein hypolipidemic peptide,JFPHP)的稳定性,以明胶和阿拉伯胶作为壁材,JFPHP为芯材,采用复凝聚法制备JFPHP微胶囊.以包埋率为指标,在单因素实验基础上,采用响应面实验优化JFPHP微胶囊制备工艺,通过氨基酸组成分析、扫描电镜、粒径分析、傅里叶红外光谱、热重分析和圆二色谱表征其性能.结果表明:复凝聚法制备JFPHP微胶囊最佳工艺条件为pH值4.10,壁材比(明胶/阿拉伯胶)为1∶1.2,壁芯比为1∶1.5,壁材浓度为1.1%,在此条件下包埋率为87.50%,负载率达77.64%.JFPHP微胶囊中含有16种氨基酸,包括31.01%的疏水性氨基酸和25.07%的酸性氨基酸,体积平均直径D_(4,3)为38.32μm,大小较均匀.冷冻干燥后的JFPHP微胶囊为不规则的块状结构,表面粗糙且有较多的孔洞,JFPHP与明胶和阿拉伯胶组成的壁材主要以物理吸附方式结合在一起,热稳定性较高.研究结果可为高稳定性的JFPHP微胶囊的制备以及JFPHP的高值化利用提供参考.展开更多
new sesquiterpene-lactone,designated as neopentalenolactone D1(1),was isolated from the fermentation cultures of Streptomyces aureus SP-371,which is known for producing agricultural-bactericide aureonuclemycin.The che...new sesquiterpene-lactone,designated as neopentalenolactone D1(1),was isolated from the fermentation cultures of Streptomyces aureus SP-371,which is known for producing agricultural-bactericide aureonuclemycin.The chemical structure of compound 1 was determined to be a neopentapyrrole natural product with a 5/5/6 tricyclic skeleton and one amide functional group,by using spectroscopic techniques such as nuclear magnetic resonance(1D/2D NMR)and high-resolution mass spectrometry(HR-ESI-MS).Its structural information indicates that it is probably the shunt product of neopentalenolactone biosynthetic pathway.In antibacterial activity evaluations,compound 1 demonstrated no inhibitory effects against the tested strains including Escherichia coli(DH5a),Staphylococcus aureus,and Bacillus subtilis under the experimental conditions.展开更多
文摘为提高泾阳茯茶蛋白降血脂肽(Jingyang fu tea protein hypolipidemic peptide,JFPHP)的稳定性,以明胶和阿拉伯胶作为壁材,JFPHP为芯材,采用复凝聚法制备JFPHP微胶囊.以包埋率为指标,在单因素实验基础上,采用响应面实验优化JFPHP微胶囊制备工艺,通过氨基酸组成分析、扫描电镜、粒径分析、傅里叶红外光谱、热重分析和圆二色谱表征其性能.结果表明:复凝聚法制备JFPHP微胶囊最佳工艺条件为pH值4.10,壁材比(明胶/阿拉伯胶)为1∶1.2,壁芯比为1∶1.5,壁材浓度为1.1%,在此条件下包埋率为87.50%,负载率达77.64%.JFPHP微胶囊中含有16种氨基酸,包括31.01%的疏水性氨基酸和25.07%的酸性氨基酸,体积平均直径D_(4,3)为38.32μm,大小较均匀.冷冻干燥后的JFPHP微胶囊为不规则的块状结构,表面粗糙且有较多的孔洞,JFPHP与明胶和阿拉伯胶组成的壁材主要以物理吸附方式结合在一起,热稳定性较高.研究结果可为高稳定性的JFPHP微胶囊的制备以及JFPHP的高值化利用提供参考.
基金supported by the National Natural Science Foundation of China(No.22077134)。
文摘new sesquiterpene-lactone,designated as neopentalenolactone D1(1),was isolated from the fermentation cultures of Streptomyces aureus SP-371,which is known for producing agricultural-bactericide aureonuclemycin.The chemical structure of compound 1 was determined to be a neopentapyrrole natural product with a 5/5/6 tricyclic skeleton and one amide functional group,by using spectroscopic techniques such as nuclear magnetic resonance(1D/2D NMR)and high-resolution mass spectrometry(HR-ESI-MS).Its structural information indicates that it is probably the shunt product of neopentalenolactone biosynthetic pathway.In antibacterial activity evaluations,compound 1 demonstrated no inhibitory effects against the tested strains including Escherichia coli(DH5a),Staphylococcus aureus,and Bacillus subtilis under the experimental conditions.
