Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus...Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus(HR-HPV)infection.Methods Female patients(n=73)with HR-HPV infection were enrolled and divided into a high-grade squamous intraepithelial lesion(HSIL)group(n=33)and a non-HSIL(N-HSIL)group(n=40),which include low-grade squamous intraepithelial lesions and inflammation.Healthy screening subjects(n=31)with negative HR-HPV results were enrolled as a control group.We examined contemporaneous plasma and secretory cytokines from 25 study subjects to investigate the difference between systemic cytokine profiles and the local microenvironment immunity using the Wilcoxon matched-pairs signed rank test.The 12 cytokines from cervical secretions were compared between the three groups using the Mann-Whitney test,and logistic regression was used to analyze HSIL and N-HSIL.Results There were statistical differences in eight cytokines(IL-2,IL-6,TNF-α,IFN-γ,IL-1β,IL-12p70,IFN-α,and IL-8)between cervical secretion and plasma of the same patient,and seven cytokines were statistically different between the control and other two groups.We selected four independent variables(TNF-α,IFN-γ,IL-12p70,and IFN-α)commonly identified by univariate regression analysis and non-parametric tests for multivariate logistic regression analysis.Based on this model,HSIL could be predicted in patients with HR-HPV infection,with the area under the curve being 0.76.Conclusion The systemic cytokine profile cannot reflect the local microenvironment immunity,and the occurrence of HSIL is related to the cytokine levels in the cervical microenvironment.展开更多
In this study,we examine the dynamics and microphysical structures of a deep compact thunderstorm event driving cloud-to-ground(CG)lightning over the Nanjing area located within the Yangtze-Huai River Basin(YHRB)durin...In this study,we examine the dynamics and microphysical structures of a deep compact thunderstorm event driving cloud-to-ground(CG)lightning over the Nanjing area located within the Yangtze-Huai River Basin(YHRB)during the monsoon break period.The microphysical structures combined with the dynamics in the glaciated,mixed-phase,and warm-phase layers during the formative,intensifying,and mature stages of the thunderstorm were first investigated using C-band polarimetric radar and CG lightning observations.The results show that the mature phase of the thunderstorm produced a local cold pool,which collided with a southerly warm wind,resulting in a strong updraft.The strong updraft favored the lifting of raindrops to the mixed-phase region to form abundant supercooled liquid water and graupel.From the formative stage to the developing stage and further to the mature stage,increased ZH-and reduced ZDR-values within the mixed-phase region are found,especially within the strong updraft region(>5 m s^(-1)).This phenomenon suggests that supercooled raindrops evolved into large hydrometeors(graupel and hail),indicative of a strong riming process.The signatures within this region are consistent with a favorable environment for thunderstorm electrification and generate the most frequent lightning during the thunderstorm life cycle.展开更多
基金supported by the National Key Research and Development Program of China(2023YFC2308800)the Natural Science Foundation of Shanghai(25ZR1402053)the Key Discipline of Public Health of the Shanghai Municipal Health Commission(Grant No.GWVI-11.1-07).
文摘Objective The aim of this study was to analyze the correlation between the levels of 12 cytokines in the cervical microenvironment and cervical intraepithelial neoplasia in patients with high-risk human papillomavirus(HR-HPV)infection.Methods Female patients(n=73)with HR-HPV infection were enrolled and divided into a high-grade squamous intraepithelial lesion(HSIL)group(n=33)and a non-HSIL(N-HSIL)group(n=40),which include low-grade squamous intraepithelial lesions and inflammation.Healthy screening subjects(n=31)with negative HR-HPV results were enrolled as a control group.We examined contemporaneous plasma and secretory cytokines from 25 study subjects to investigate the difference between systemic cytokine profiles and the local microenvironment immunity using the Wilcoxon matched-pairs signed rank test.The 12 cytokines from cervical secretions were compared between the three groups using the Mann-Whitney test,and logistic regression was used to analyze HSIL and N-HSIL.Results There were statistical differences in eight cytokines(IL-2,IL-6,TNF-α,IFN-γ,IL-1β,IL-12p70,IFN-α,and IL-8)between cervical secretion and plasma of the same patient,and seven cytokines were statistically different between the control and other two groups.We selected four independent variables(TNF-α,IFN-γ,IL-12p70,and IFN-α)commonly identified by univariate regression analysis and non-parametric tests for multivariate logistic regression analysis.Based on this model,HSIL could be predicted in patients with HR-HPV infection,with the area under the curve being 0.76.Conclusion The systemic cytokine profile cannot reflect the local microenvironment immunity,and the occurrence of HSIL is related to the cytokine levels in the cervical microenvironment.
基金primarily supported by the National Natural Science Foundation of China(Grant Nos.42025501,41805025,42175005,and 61827901)the National Key R&D Program of China(2022YFC3003905)+5 种基金the National Key Laboratory on Electromagnetic Environmental Effects and Electro-optical Engineering(NO.JCKYS61422062101)the Meteorological Union Fund of the National Natural Science Foundation of China(U2142203)the Foundation of Jiangsu Provincial Meteorological Bureau(KM202308)The Open Grants of China Meteorological Administration Radar Meteorology Key Laboratory(2023LRMB04)S&T Development Fund of NJIAS(KJF202307)the Open Research Program of the State Key Laboratory of Severe Weather(2022LASW-A01)。
文摘In this study,we examine the dynamics and microphysical structures of a deep compact thunderstorm event driving cloud-to-ground(CG)lightning over the Nanjing area located within the Yangtze-Huai River Basin(YHRB)during the monsoon break period.The microphysical structures combined with the dynamics in the glaciated,mixed-phase,and warm-phase layers during the formative,intensifying,and mature stages of the thunderstorm were first investigated using C-band polarimetric radar and CG lightning observations.The results show that the mature phase of the thunderstorm produced a local cold pool,which collided with a southerly warm wind,resulting in a strong updraft.The strong updraft favored the lifting of raindrops to the mixed-phase region to form abundant supercooled liquid water and graupel.From the formative stage to the developing stage and further to the mature stage,increased ZH-and reduced ZDR-values within the mixed-phase region are found,especially within the strong updraft region(>5 m s^(-1)).This phenomenon suggests that supercooled raindrops evolved into large hydrometeors(graupel and hail),indicative of a strong riming process.The signatures within this region are consistent with a favorable environment for thunderstorm electrification and generate the most frequent lightning during the thunderstorm life cycle.
