3-Deoxy-D-manno–oct-2-ulosonic acid(Kdo)is widely distributed in bacteria,and the synthesis of Kdocontaining oligosaccharides is important for the development of novel antibiotics and immunological agents.We have rec...3-Deoxy-D-manno–oct-2-ulosonic acid(Kdo)is widely distributed in bacteria,and the synthesis of Kdocontaining oligosaccharides is important for the development of novel antibiotics and immunological agents.We have recently developed a strategy to achieveα-stereocontrolled glycosylation using a C3-p-tolylthio-substituted Kdo phosphite donor.The wide substrate scope and high reactivity of the donors enabled the efficient synthesis of a series of Kdo-containing glycosides with completeα-stereoselectivity and without the formation of 2,3-ene byproducts.In this study,we improved the method by replacing the leaving group diethyl phosphite with fluoride,which enhanced the stability of the donor and led to cleaner reaction.Furthermore,the substrate range was expanded by synthesizing a series of Kdo O/C/S/Nglycosides,which also opened up a new avenue for the synthesis of CMP-Kdo synthase inhibitors.展开更多
Kdo residues are widely distributed in bacteria.They are components of bacterial lipopolysaccharide(LPS)and capsular polysaccharides,which can be recognized by the human adaptive immune system,and have great potential...Kdo residues are widely distributed in bacteria.They are components of bacterial lipopolysaccharide(LPS)and capsular polysaccharides,which can be recognized by the human adaptive immune system,and have great potential for developing new sugar chips and antibacterial vaccines.By improving the existing methods,we optimized the Kdo chemical synthesis method,which was able to efficiently synthesize Kdo monosaccharides with high purity,laying a foundation for the construction of subsequent compound libraries.Kdo acylation can easily form 1,5-lactones and reduce the efficiency of synthesis.By changing the synthesis sequence,we avoided the formation of Kdo 1,5-lactones,improved the synthesis efficiency of Kdo glycals,and provided a new strategy for derivatization.展开更多
Endoplasmic reticulum(ER)viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF).Herein,we report the first ER-targeting viscosity-responsive near-infrared(NIR)fluorescent probe HBT-PP for investig...Endoplasmic reticulum(ER)viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF).Herein,we report the first ER-targeting viscosity-responsive near-infrared(NIR)fluorescent probe HBT-PP for investigations of HF pathogenesis.The twisted intramolecular charge transfer(TICT)and the excited state intramolecular proton transfer(ESIPT)are combined to endow HBT-PP with the selective response towards viscosity(~62-fold)and a super large stokes shift(~320 nm).HBT-PP mainly targets organelle ER and is able to image the drug-or alcohol-induced viscosity elevation in living cells.Significantly,HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression.Of note,after 10 min of stain with HBT-PP,HF lesions showed a stage-based response,indicating the potential of this probe in fast and early diagnosis of HF.These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.展开更多
This paper proposes a method that combines etching,masking,and magnetron sputtering techniques to prepare a ring-shaped silver mirror structure on the end face of a standard multimode optical fiber.By utilizing the ca...This paper proposes a method that combines etching,masking,and magnetron sputtering techniques to prepare a ring-shaped silver mirror structure on the end face of a standard multimode optical fiber.By utilizing the capillary effect to fill a capillary glass tube with polydimethylsiloxane(PDMS),a structure consisting of a PDMS cavity and an air cavity is made,resulting in the achievement of a fiber optic dual Fabry–Perot(F–P)cavity temperature sensor with a ring-shaped silver mirror structure.When the external temperature changes,both the cavity length and the refractive index of PDMS change,causing variations in the intensity of interference light.The annular silver mirror utilizes its high reflectivity to allow more light to enter the receiving end,resulting in a more pronounced change in photon count.Within the range of 25–80℃,sensitivity of 150.74 cps/℃ and linearity of 0.998 have been achieved.Our optical fiber temperature sensor has demonstrated cost-effectiveness,wide range,and high stability in temperature detection through multiple repeated experiments.展开更多
基金supported by the National Key R&D Program of China(Nos.2022YFF1203005,2022YFC2303700)supported by the National Natural Science Foundation of China(Nos.81930097,82151223).
文摘3-Deoxy-D-manno–oct-2-ulosonic acid(Kdo)is widely distributed in bacteria,and the synthesis of Kdocontaining oligosaccharides is important for the development of novel antibiotics and immunological agents.We have recently developed a strategy to achieveα-stereocontrolled glycosylation using a C3-p-tolylthio-substituted Kdo phosphite donor.The wide substrate scope and high reactivity of the donors enabled the efficient synthesis of a series of Kdo-containing glycosides with completeα-stereoselectivity and without the formation of 2,3-ene byproducts.In this study,we improved the method by replacing the leaving group diethyl phosphite with fluoride,which enhanced the stability of the donor and led to cleaner reaction.Furthermore,the substrate range was expanded by synthesizing a series of Kdo O/C/S/Nglycosides,which also opened up a new avenue for the synthesis of CMP-Kdo synthase inhibitors.
基金National Natural Science Foundation of China(Grant No.81930097,21977005)。
文摘Kdo residues are widely distributed in bacteria.They are components of bacterial lipopolysaccharide(LPS)and capsular polysaccharides,which can be recognized by the human adaptive immune system,and have great potential for developing new sugar chips and antibacterial vaccines.By improving the existing methods,we optimized the Kdo chemical synthesis method,which was able to efficiently synthesize Kdo monosaccharides with high purity,laying a foundation for the construction of subsequent compound libraries.Kdo acylation can easily form 1,5-lactones and reduce the efficiency of synthesis.By changing the synthesis sequence,we avoided the formation of Kdo 1,5-lactones,improved the synthesis efficiency of Kdo glycals,and provided a new strategy for derivatization.
基金supported by the National Natural Science Foundation of China(22338005,22208045,2192580,U23A20489)the Liaoning Binhai Laboratory(LBLB-2023-03)+2 种基金the Fundamental Research Funds for the Central Universities(DUT22LAB60)the Zhejiang Provincial Natural Science Foundation(TGY24B060002)the Ningbo Natural Science Foundation(2022J012,2023S115)。
文摘Endoplasmic reticulum(ER)viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF).Herein,we report the first ER-targeting viscosity-responsive near-infrared(NIR)fluorescent probe HBT-PP for investigations of HF pathogenesis.The twisted intramolecular charge transfer(TICT)and the excited state intramolecular proton transfer(ESIPT)are combined to endow HBT-PP with the selective response towards viscosity(~62-fold)and a super large stokes shift(~320 nm).HBT-PP mainly targets organelle ER and is able to image the drug-or alcohol-induced viscosity elevation in living cells.Significantly,HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression.Of note,after 10 min of stain with HBT-PP,HF lesions showed a stage-based response,indicating the potential of this probe in fast and early diagnosis of HF.These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.
基金supported by the National Natural Science Foundation of China(Nos.62365015,61765012,51965053)the Natural Science Foundation of Inner Mongolia(Nos.2023MS05047,2019MS05008,2020MS05036)+4 种基金the Fundamental Research Funds for Inner Mongolia University of Science&Technology(Nos.2023RCTD011,2023YXXS012)the National Key Research and Development Program of China(Nos.2017YFF0207200,2017YFF0207203)the Research Program of Science and Technology at Universities of Inner Mongolia Autonomous Region(Nos.2017CXYD-2,KCBJ2018031)the Inner Mongolia University of Science and Technology Youth Fund Project(No.202/0303022006)the Scientific Research Program of Higher Education Institutions in Inner Mongolia Autonomous Region(No.NJZY23082)。
文摘This paper proposes a method that combines etching,masking,and magnetron sputtering techniques to prepare a ring-shaped silver mirror structure on the end face of a standard multimode optical fiber.By utilizing the capillary effect to fill a capillary glass tube with polydimethylsiloxane(PDMS),a structure consisting of a PDMS cavity and an air cavity is made,resulting in the achievement of a fiber optic dual Fabry–Perot(F–P)cavity temperature sensor with a ring-shaped silver mirror structure.When the external temperature changes,both the cavity length and the refractive index of PDMS change,causing variations in the intensity of interference light.The annular silver mirror utilizes its high reflectivity to allow more light to enter the receiving end,resulting in a more pronounced change in photon count.Within the range of 25–80℃,sensitivity of 150.74 cps/℃ and linearity of 0.998 have been achieved.Our optical fiber temperature sensor has demonstrated cost-effectiveness,wide range,and high stability in temperature detection through multiple repeated experiments.
