摘要
Endoplasmic reticulum(ER)viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF).Herein,we report the first ER-targeting viscosity-responsive near-infrared(NIR)fluorescent probe HBT-PP for investigations of HF pathogenesis.The twisted intramolecular charge transfer(TICT)and the excited state intramolecular proton transfer(ESIPT)are combined to endow HBT-PP with the selective response towards viscosity(~62-fold)and a super large stokes shift(~320 nm).HBT-PP mainly targets organelle ER and is able to image the drug-or alcohol-induced viscosity elevation in living cells.Significantly,HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression.Of note,after 10 min of stain with HBT-PP,HF lesions showed a stage-based response,indicating the potential of this probe in fast and early diagnosis of HF.These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.
基金
supported by the National Natural Science Foundation of China(22338005,22208045,2192580,U23A20489)
the Liaoning Binhai Laboratory(LBLB-2023-03)
the Fundamental Research Funds for the Central Universities(DUT22LAB60)
the Zhejiang Provincial Natural Science Foundation(TGY24B060002)
the Ningbo Natural Science Foundation(2022J012,2023S115)。
