The purpose of the study was to establish an analytical method to simultaneously determine multiple sterols in tobacco leaves rapidly and accurately.In this gas chromatography-triple quadruple tandem mass spectrometry...The purpose of the study was to establish an analytical method to simultaneously determine multiple sterols in tobacco leaves rapidly and accurately.In this gas chromatography-triple quadruple tandem mass spectrometry(GC-MS/MS)based method,various conditions for sterols extraction were assessed and the instrumental operation parameters were optimized with 5α-cholane as an internal standard.Using this method,eight plant sterols,namely lanosterol,campesterol,chenodeoxycholic acid,cholesterol,β-sitosterol,stigmasterol,dihydrotachysterol and ergosterol,were separated in less than 30 min.The linear correlation coefficients were over 0.998 9,the low detection limits were in the range of 0.010 3-0.141 4 μg/g.The recoveries were from 87.30% to 115.60%,with low standard deviations.In conclusion,this method demonstrates good repeatability,accuracy,and high sensitivity,and is best suited for rapid analysis of multiple sterols in tobacco leaves.展开更多
文摘目的通过系统检索并整合分析中医药治疗前列腺癌的临床随机对照试验,对中医药治疗前列腺癌的整体证据分布进行归纳。方法计算机检索中国知网、万方数据库、维普数据库、中国生物医学文献服务系统、Pub Med、EMbase、Cochrane Library、Web of Science等数据库收录的中医药治疗前列腺癌的文献,检索时间为建库至2024年6月,提取并总结相关信息。结果共纳入115篇临床随机对照试验文献,年发文量总体呈上升趋势,但多数研究规模较小,样本量集中为50~<100例;干预疗程多为4~12周;治疗组以中西医治疗为主,口服中药汤剂是最常用的干预措施;观察指标以前列腺特异性抗原、体力状况、生活质量、不良反应等为主,对中医证候评分、病灶大小、疾病进展等指标的关注度较少。结论中医药治疗前列腺癌的优势良好,但多数文献研究质量一般,建议扩大样本量,对观察周期及临床证型等进一步规范,提高研究质量,以验证中医药治疗前列腺癌的独特疗效。
文摘The purpose of the study was to establish an analytical method to simultaneously determine multiple sterols in tobacco leaves rapidly and accurately.In this gas chromatography-triple quadruple tandem mass spectrometry(GC-MS/MS)based method,various conditions for sterols extraction were assessed and the instrumental operation parameters were optimized with 5α-cholane as an internal standard.Using this method,eight plant sterols,namely lanosterol,campesterol,chenodeoxycholic acid,cholesterol,β-sitosterol,stigmasterol,dihydrotachysterol and ergosterol,were separated in less than 30 min.The linear correlation coefficients were over 0.998 9,the low detection limits were in the range of 0.010 3-0.141 4 μg/g.The recoveries were from 87.30% to 115.60%,with low standard deviations.In conclusion,this method demonstrates good repeatability,accuracy,and high sensitivity,and is best suited for rapid analysis of multiple sterols in tobacco leaves.
