This study examined the effect of dietary serine(Ser)on egg white quality,ovomucin content of laying hens fed low-gossypol cottonseed meal(LCSM)-based diet and sought to explore the regulatory mechanism underlying the...This study examined the effect of dietary serine(Ser)on egg white quality,ovomucin content of laying hens fed low-gossypol cottonseed meal(LCSM)-based diet and sought to explore the regulatory mechanism underlying the effects.A total of 288 Hy-Line Brown layers were randomly assigned into 3 treatments and respectively offered soybean meal(SBM)-based diet,LCSM-based diet,and LCSM-based diet supplemented with 0.435%L-Ser.Dietary Ser supplementation reversed the decrease in quality indices of albumen,including the proportion of thick albumen(P<0.05),thick-to-thin ratio(P<0.05),albumen height(P<0.05),Haugh unit(P<0.05),and apparent viscosity that were caused by LCSM intake.Also,dietary Ser supplementation mitigated the LCSM-induced reduction in ovomucin content of the albumen(P<0.05).FT-IR analysis of ovomucin revealed differences in second derivative spectra at 1,200-800 cm^(-1) between birds received SBM-and LCSM-based diets,meanwhile,the α-helix of ovomucin was decreased by LCSM but increased by Ser addition(P<0.05).Furthermore,Ser addition up-regulated the mRNA expression of β-ovomucin in magnum mucosa at 2 and 7.5 h post-oviposition(P<0.05).Ser mitigated the LCSMinduced impairment of magnum epithelium,increased goblet cell counts(P<0.05),and up-regulated the expression of occludin,mucin 2,and the relevant glycosyltransferases for O-glycosylation in magnum mucosa(P<0.05).Conclusively,the alleviating effect of Ser on declining albumen quality due to dietary LCSM,could be explained by the efficacy of Ser in regulating ovomucin synthesis.This regulation occurred at the levels of transcription and posttranscriptional O-glycosylation modification ofβ-ovomucin,while positive effect on magnum morphology and barrier function could also in part account for the ovomucin content.This would provide a promising research direction on the adoption of nutritional interventions for manipulation of egg white quality from perspectives of albumen protein synthesis.展开更多
Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.Thi...Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.This study investigates the photochemical behavior of Fe(III)-PROC complexes using alkaline lignin(AL),betaine hydrochloride(BH),and phytic acid(PA)as representative proxies for PROC.The reductive agent AL demonstrated the ability to directly reduce Fe(III)to Fe(II).In contrast,BH,being unable to form strong complexes with Fe(III),was able to quench·OH,thereby resulting in a shift of the redox equilibrium towards Fe(II).PA exhibited a strong binding affinity for Fe(III),effectively inhibiting its photoreduction.Electron paramagnetic resonance(EPR)analysis,utilizing 5,5-dimethyl-1-pyrroline-N-oxide(DMPO)as a spin trap,revealed that the DMPO-OH signal detected in photolyzed Fe(III)-PROC solutions originated from various pathways.Specifically,uncomplexed Fe(III)in AL or BH solutions was shown to oxidize DMPO directly,leading to the formation of a false DMPO-OH adduct.The addition of ethanol to the photolyzed Fe(III)-AL and Fe(III)-BH systems resulted in the generation of the DMPO-CH(CH_(3))OH adduct,thereby confirming the presence of authentic·OH in these systems.The photolysis of the Fe(III)-PA complex may proceed via a photodissociation mechanism,where the resulting loosely bound Fe(III)can oxidize DMPO,followed by a nucleophilic attack from water.This research highlights the multifaceted roles of PROC in facilitating the redox cycling of iron within soil and aquatic ecosystems.展开更多
Background This study investigated the molecular mechanisms by which redox status regulates protoporphyrin IX(PpIX)biosynthesis and eggshell coloration in brown-shelled laying hens.This study consisted of two experime...Background This study investigated the molecular mechanisms by which redox status regulates protoporphyrin IX(PpIX)biosynthesis and eggshell coloration in brown-shelled laying hens.This study consisted of two experiments involving 48 and 32 healthy 60-week-old Hy-Line Brown hens,respectively.The hens exhibited either dark(L*=51.99±2.08)or light(L*=64.12±3.02)brown eggshell colors.In Exp.1,light brown-shelled hens were fed a basal diet(Lb group),while dark brown-shelled hens received either a basal diet(Db group)or a basal diet with 10 mg/kg ammonium metavanadate(Dbv group)for 20 d.In Exp.2,light brown-shelled hens received either a basal diet(Lbc group)or a basal diet supplemented with 200 mg/kg resveratrol(Lbr group)for 12 weeks.Results Compared to the Db group,eggshell L*values increased,and PpIX concentrations in both eggshell and uterus decreased in Dbv and Lb groups.These groups also showed oxidative stress,as indicated by reduced hepatic T-SOD and CAT activities.Uterine redox status changes were further confirmed by increased T-AOC level(Dbv)and reduced CAT gene expression(Lb).These redox disturbances led to reduced expression of ND4 and COX1 mt DNA,decreased ATP production and CS activity,along with upregulation of IR,PI3K,HK,and PK gene expression,reflecting altered mitochondrial energy metabolism.Notably,the SIRT1/PGC-1αsignaling cascade and its downstream target ALAS1 were significantly downregulated at both mRNA and protein levels in Dbv and Lb groups.Compared to the Lbc group,the Lbr group exhibited higher antioxidant capacity by increasing hepatic CAT activity and uterine T-SOD and GSH-Px activities,and reducing MDA levels.Moreover,the Lbr group restored mitochondrial function and PpIX biosynthesis by upregulating ND4 and COX1 mt DNA,CS and SDHA gene expression,and SIRT1/PGC-1α/ALAS1 signaling,while downregulating LDH activity and the expression of IR and PI3K,thereby alleviating eggshell color fading.Conclusion Oxidative stress induces eggshell depigmentation by impairing mitochondrial function and downregulating the SIRT1/PGC-1α/ALAS1 pathway,leading to reduced PpIX biosynthesis.Specifically,vanadium-induced or endogenous oxidative stress disrupts mitochondrial energy metabolism and suppresses key components of this pathway,while resveratrol alleviates oxidative damage and restores mitochondrial function and ALAS1-driven PpIX synthesis through reactivation of the SIRT1/PGC-1αaxis.展开更多
Arc ion plating (AIP) is applied to form Ti/(Ti,Cr)N/CrN multilayer coating on the surface of 316L stainless steel (SS316L) as bipolar plates for proton exchange membrane fuel cells (PEMFCs). The characterizations of ...Arc ion plating (AIP) is applied to form Ti/(Ti,Cr)N/CrN multilayer coating on the surface of 316L stainless steel (SS316L) as bipolar plates for proton exchange membrane fuel cells (PEMFCs). The characterizations of the coating are analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Interfacial contact resistance (ICR) between the coated sample and carbon paper is 4.9 m Omega cm(2) under 150 N/cm(2), which is much lower than that of the SS316L substrate. Potentiodynamic and potentiostatic tests are performed in the simulated PEMFC working conditions to investigate the corrosion behaviors of the coated sample. Superior anticorrosion performance is observed for the coated sample, whose corrosion current density is 0.12 mu A/cm(2). Surface morphology results after corrosion tests indicate that the substrate is well protected by the multilayer coating. Performances of the single cell with the multilayer coated SS316L bipolar plate are improved significantly compared with that of the cell with the uncoated SS316L bipolar plate, presenting a great potential for PEMFC application. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.展开更多
Colloform pyrite with core-rim texture is commonly deposited in carbonate platforms associated with the sulfide ores such as the Caixiashan Pb-Zn deposit.However,the genesis of colloform pyrite in Pb-Zn deposits,its g...Colloform pyrite with core-rim texture is commonly deposited in carbonate platforms associated with the sulfide ores such as the Caixiashan Pb-Zn deposit.However,the genesis of colloform pyrite in Pb-Zn deposits,its growth controls and their geological implication are insufficiently understood.Integration of in-situ trace element and SIMS sulfur isotopes has revealed geochemical variations among these pyrite layers.These colloform pyrite occur as residual phases of core-rim aggregates,the cores are made up of very fine-grained anhedral pyrite particles,with some rims being made up of fine-grained and poorlycrystallized pyrite,while the other rims were featured with euhedral cubic pyrite.which are cemented by fine-grained calcite and/or dolomite with minor quartz.Sulfur isotope analysis shows that some wellpreserved rims have negative δ^34 S values(-28.12‰to-0.49‰),whereas most of the cores and rims have positive δ^34 S values(>0 to+44.28‰;peak at+14.91‰).Integrating with the methane and sulfate were observed in previous fluid inclusion study,we suggest that the 34 S depleted rims were initially formed by bacteria sulfate reduction(BSR),whereas the positive δ^34 S values were resulted from the sulfate reduction driven by anaerobic methane oxidation(AOM).The well-developed authigenic pyrite and calcite may also support the reaction of AOM.Combined with petrographic observations,trace element composition of the colloform pyrite reveals the incorporation and precipitation behavior of those high abundance elements in the pyrite:Pb and Zn were present as mineral inclusion and likely precipitated before Fe,as supported by the time-resolved Pb-Zn signal spikes in most of the analyzed pyrite grains.Other metals,such as Hg,Co and Ni,may have migrated as chloride complexes and entered the pyrite lattice.Arsenic and Sb,generally influenced by complex-forming reactions rather than substitution ones,could also enter the pyrite lattice,or slightly predate the precipitation of colloform pyrite as mineral inclusions,which are controlled by their hydrolysis constant in the ore fluids.The colloform pyrite may have grown inward from the rims.The successive BSR reaction process would enrich H^32/2S in the overlying water column but reduce the metal content,the nucleation of these pyrite rims was featured by strongly negative sulfur isotopes.The following AOM process should be activated by deformation like the turbidity sediment of the mudstone as the sulfide deposition are associated with fault activities that caused the emission of methane migration upward and simultaneously replenishing the metal in the column.The higher AOM reaction rate and the higher metal supply(not only Fe.but with minor other metals such as Pb and Zn) caused by sediment movement enhanced the metal concentration within the pyrite lattice.展开更多
Collagen is the building component of temporomandibular joint(TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inf...Collagen is the building component of temporomandibular joint(TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However,the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague–Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm^(-1)/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs,indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs.展开更多
Hydrogen embrittlement behavior, micro-deformation, and crack propagation mechanism of CoCrFeNiMn high-entropy alloy (HEA) fabricated by laser powder bed fusion (LPBF) under different parameters were investigated by s...Hydrogen embrittlement behavior, micro-deformation, and crack propagation mechanism of CoCrFeNiMn high-entropy alloy (HEA) fabricated by laser powder bed fusion (LPBF) under different parameters were investigated by slow strain rate tensile tests (at room temperature) with/without electrochemical hydrogen pre-charging. The LPBF CoCrFeNiMn HEA shows excellent resistance to hydrogen embrittlement. Unsuitable LPBF parameters are accompanied by many microcracks and holes, resulting in a slight decrease in the hydrogen embrittlement resistance of the material. The electron backscatter diffraction (EBSD), electron channeling contrast image (ECCI) techniques, and transmission electron microscope (TEM) were carried out to research the main influencing factors of hydrogen on the deformation mechanism and crack propagation. Compared with un-charged samples, a larger number of deformation twins (DTs) appear in the deformation process of hydrogen-charged LPBF CoCrFeNiMn, attributing to the reduction of stacking fault energy (SFE) due to the ingress of hydrogen. The nano DTs and crossing twin system contribute to the extra work hardening, and a strain hardening platform is observed for all hydrogen-charged samples, resulting in the increase of strain hardening rate or the mitigation of the loss of strain hardening. Although unsuitable process parameters will trigger fabrication defects and reduce mechanical properties, the cellular structure can bring a hydrogen-induced strain hardening platform for LPBF CoCrFeNiMn to reduce the damage caused by hydrogen embrittlement.展开更多
Low desulfurization efficiency impedes the wide application of dry desulfurization technology,which is a low-cost and simple process,and one significant solution is the development and manufacture of high-performance ...Low desulfurization efficiency impedes the wide application of dry desulfurization technology,which is a low-cost and simple process,and one significant solution is the development and manufacture of high-performance desulfurizers.In this study,firstly,a steam jet mill was used to digest quicklime;then,we utilized numerical simulation to study the flow field distribution and analyze the driving factors of quicklime digestion;and lastly,the desulfurization performance of the desulfurizer was evaluated under different relative humidities.The results show that the desulfurizer prepared via the steam jet mill had better apparent activity than traditional desulfurizers.Also,the entire jet flow field of the steam jet mill is in a supersonic and highly turbulent flow state,with high crushing intensity and good particle acceleration performance.Sufficient contact with the nascent surface maximizes the formation of slaked lime.The experiments demonstrated that the operating time with 100%desulfurization efficiency and the“break-through”time for the desulfurizer prepared via the steam jet mill is longer than that of traditional desulfurizers,and has significant advantages,especially at low flue gas relative humidity.Compared with traditional desulfurizers,the desulfurizer prepared via steam jet mill expands the range of acceptable flue gas temperature,and the failure temperature is 1.625 times that of traditional desulfurizers.This work breaks through the technical bottleneck of low dry desulfurization efficiency,which is an important step in pushing forward the application of dry desulfurization.展开更多
Effects of nucleation sites and diffusivity enhancement of chromium on reverted transformation of AISI 304 stainless steel during annealing process were investigated.Dynamics calculation revealed that the reverted tra...Effects of nucleation sites and diffusivity enhancement of chromium on reverted transformation of AISI 304 stainless steel during annealing process were investigated.Dynamics calculation revealed that the reverted transformation of strain-inducedα’-martensite→γaustenite could were closely associated with active nucleation sites and diffusivity enhancement of chromium in nanocrystallineα’-martensite.The experimental data and the results were in accordance with 2-grain austenite/α’-martensite junctions calculated theoretically,which could result from high chromium diffusion rate in nanocrystallineα’-martensite.In addition,low temperature is not conducive to reversed transformation,while high temperature and long annealing time will lead to inhomogeneous grain size distribution.展开更多
Future education is highly integrated with technology and highly open education.The rapid development of information technology urges education to embrace the change brought by technology enthusiastically.The alienati...Future education is highly integrated with technology and highly open education.The rapid development of information technology urges education to embrace the change brought by technology enthusiastically.The alienation of thinking under technology package must not be ignored and guard against the tendency of"technology determinism".Therefore,this paper interprets the irreplaceable life education from three angles:the reform trend,the basic pattern and the practice construction,and makes full use of technology to restore the meaning of life at the same time.展开更多
Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanism...Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.展开更多
Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic mod...Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic modulus makes Elinvar alloys highly desirable in precision-control applications,including aerospace,electronics,and optical instruments.Currently,most of the used and studied Elinvar alloys are ferromagnetic alloys(FeNi and Fe-Pt)and antiferromagnetic alloys(Fe-Mn and γ-MnCu)[2–4].The Elinvar effect in these alloys typically originates from magnetostriction or magnetoelastic effects,which are magnetic fieldor magnetic transition-dependent[5].Consequently,these Elinvar alloys cannot function properly in the presence of a magnetic field owing to their Elinvar effect being closely tied to magnetic phase transition.Therefore,developing non-magneticdependent Elinvar alloys is highly essential to widen their practical applications.展开更多
Plp1-lineage Schwann cells(SCs)of peripheral nerve play a critical role in vascular remodeling and osteogenic differentiation during the early stage of bone healing,and the abnormal plasticity of SCs would jeopardize ...Plp1-lineage Schwann cells(SCs)of peripheral nerve play a critical role in vascular remodeling and osteogenic differentiation during the early stage of bone healing,and the abnormal plasticity of SCs would jeopardize the bone regeneration.However,how Plp1-lineage cells respond to injury and initiate the vascularized osteogenesis remains incompletely understood.Here,by employing single-cell transcriptional profiling combined with lineage-specific tracing models,we uncover that Plp1-lineage cells undergoing injury-induced glia-to-MSCs transition contributed to osteogenesis and revascularization in the initial stage of bone injury.Importantly,our data demonstrated that the Sonic hedgehog(Shh)signaling was responsible for the transition process initiation,which was strongly activated by c-Jun/SIRT6/BAF170 complex-driven Shh enhancers.Collectively,these findings depict an injuryspecific niche signal-mediated Plp1-lineage cells transition towards Gli1+MSCs and may be instructive for approaches to promote bone regeneration during aging or other bone diseases.展开更多
The largest Tan-Lu active fault system in northeastern Asia,spans approximately 3500 km in length and varies in width from 10 km to 200 km.In 1668,an earthquake with a magnitude of 8.5 occurred in Tancheng,causing the...The largest Tan-Lu active fault system in northeastern Asia,spans approximately 3500 km in length and varies in width from 10 km to 200 km.In 1668,an earthquake with a magnitude of 8.5 occurred in Tancheng,causing the loss of over 50000 lives.To constrain the timing and process of the Tan-Lu fault system on eastern Asian margin,this study presents the field mapping,thin section observation,geochronology,and microanalysis of Weiyuanpu-Yehe ductile shear zone(WYSZ)of the northern Tan-Lu fault system.Kinematic indicators and microstructures suggest a sense of sinistral strike-slip.The deformation temperature of the mylonite is mediate to high based on the quartz deformation,c-axis fabrics.The differential stress of the shear zone is 20‒40 MPa using quartz paleopiezometry.The dikes within the shear zone yielded zircon U-Pb ages of 165‒163 Ma.However,due to the ambiguous geological relationship between the dikes and shear zone,additional geochronology is warranted.Since the Mesozoic era,based on the exposure of mylonite and dikes,the upper crust has been extensively eroded,exposing the ductile shear zone.Moreover,the understanding of the geometry and process of pre-existing structures has fundamental implications for predicating the potential earthquakes for the Tan-Lu fault system.展开更多
Metastable β-Ti alloys exhibiting twinning-induced plasticity (TWIP) and transformation-induced plasticity (TRIP) generally have excellent ductility, but typically at the expense of relatively low yield strengths whi...Metastable β-Ti alloys exhibiting twinning-induced plasticity (TWIP) and transformation-induced plasticity (TRIP) generally have excellent ductility, but typically at the expense of relatively low yield strengths which has restricted their widespread use. Our work shows that interstitial oxygen can be employed to regulate β phase stability to significantly enhance both strength and ductility of TWIP/TRIP alloys. For a Ti-32Nb wt.% base alloy, inclusion of 0.3 wt.% O enhanced ductility by more than 140 %, reaching up to 54 % strain, and improved the tensile yield strength by over 95 % to 632 MPa. Compared to other common engineering alloys such as Ti-45Nb, elongation was increased by 29 %, and the yield strength increased by 182 MPa, respectively. Here, we elucidate on impacts of oxygen doping on TWIP/TRIP behaviors in the Ti-32Nb alloy. We reveal that oxygen regulates the critical stress for martensitic transformation, twinning, and dislocation slip. At lower oxygen doping concentrations (≤0.3 wt.% O), multi-stage martensitic transformation and martensitic twinning resulted in high ductility. In higher oxygen content alloys (≥0.5 wt.% O), deformation occurred initially via twinning, while strain induced martensite was subsequently induced in retained β phase regions. Oxygen concentrations control the deformation mechanisms, providing a flexible means to synergistically balance an alloy's strength and ductility. The use of oxygen to enhance stability of the β phase and regulate deformation behaviors is a promising new approach for creating high-performance TWIP/TRIP metastable β-Ti alloys with outstanding mechanical properties.展开更多
基金financially supported by the National Natural Science Foundation of China(32072774)the China Postdoctoral Science Foundation(2023M733822)+2 种基金the Beijing Innovation Consortium of Agriculture Research System(BAIC04),Chinathe earmarked fund for CARS National System for Layer Production Technology,China(CARS-40)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences。
文摘This study examined the effect of dietary serine(Ser)on egg white quality,ovomucin content of laying hens fed low-gossypol cottonseed meal(LCSM)-based diet and sought to explore the regulatory mechanism underlying the effects.A total of 288 Hy-Line Brown layers were randomly assigned into 3 treatments and respectively offered soybean meal(SBM)-based diet,LCSM-based diet,and LCSM-based diet supplemented with 0.435%L-Ser.Dietary Ser supplementation reversed the decrease in quality indices of albumen,including the proportion of thick albumen(P<0.05),thick-to-thin ratio(P<0.05),albumen height(P<0.05),Haugh unit(P<0.05),and apparent viscosity that were caused by LCSM intake.Also,dietary Ser supplementation mitigated the LCSM-induced reduction in ovomucin content of the albumen(P<0.05).FT-IR analysis of ovomucin revealed differences in second derivative spectra at 1,200-800 cm^(-1) between birds received SBM-and LCSM-based diets,meanwhile,the α-helix of ovomucin was decreased by LCSM but increased by Ser addition(P<0.05).Furthermore,Ser addition up-regulated the mRNA expression of β-ovomucin in magnum mucosa at 2 and 7.5 h post-oviposition(P<0.05).Ser mitigated the LCSMinduced impairment of magnum epithelium,increased goblet cell counts(P<0.05),and up-regulated the expression of occludin,mucin 2,and the relevant glycosyltransferases for O-glycosylation in magnum mucosa(P<0.05).Conclusively,the alleviating effect of Ser on declining albumen quality due to dietary LCSM,could be explained by the efficacy of Ser in regulating ovomucin synthesis.This regulation occurred at the levels of transcription and posttranscriptional O-glycosylation modification ofβ-ovomucin,while positive effect on magnum morphology and barrier function could also in part account for the ovomucin content.This would provide a promising research direction on the adoption of nutritional interventions for manipulation of egg white quality from perspectives of albumen protein synthesis.
基金supported by the Natural Science Foundation of Shanghai(No.24ZR1419600).
文摘Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.This study investigates the photochemical behavior of Fe(III)-PROC complexes using alkaline lignin(AL),betaine hydrochloride(BH),and phytic acid(PA)as representative proxies for PROC.The reductive agent AL demonstrated the ability to directly reduce Fe(III)to Fe(II).In contrast,BH,being unable to form strong complexes with Fe(III),was able to quench·OH,thereby resulting in a shift of the redox equilibrium towards Fe(II).PA exhibited a strong binding affinity for Fe(III),effectively inhibiting its photoreduction.Electron paramagnetic resonance(EPR)analysis,utilizing 5,5-dimethyl-1-pyrroline-N-oxide(DMPO)as a spin trap,revealed that the DMPO-OH signal detected in photolyzed Fe(III)-PROC solutions originated from various pathways.Specifically,uncomplexed Fe(III)in AL or BH solutions was shown to oxidize DMPO directly,leading to the formation of a false DMPO-OH adduct.The addition of ethanol to the photolyzed Fe(III)-AL and Fe(III)-BH systems resulted in the generation of the DMPO-CH(CH_(3))OH adduct,thereby confirming the presence of authentic·OH in these systems.The photolysis of the Fe(III)-PA complex may proceed via a photodissociation mechanism,where the resulting loosely bound Fe(III)can oxidize DMPO,followed by a nucleophilic attack from water.This research highlights the multifaceted roles of PROC in facilitating the redox cycling of iron within soil and aquatic ecosystems.
基金financially supported by the National Natural Science Foundation of China(32322078)the earmarked fund for the China Agriculture Research System(CARS-40)+1 种基金the China Postdoctoral Science Foundation(grant no.2024M763615)the Agricultural Science and Technology Innovation Program(ASTIP)of CAAS。
文摘Background This study investigated the molecular mechanisms by which redox status regulates protoporphyrin IX(PpIX)biosynthesis and eggshell coloration in brown-shelled laying hens.This study consisted of two experiments involving 48 and 32 healthy 60-week-old Hy-Line Brown hens,respectively.The hens exhibited either dark(L*=51.99±2.08)or light(L*=64.12±3.02)brown eggshell colors.In Exp.1,light brown-shelled hens were fed a basal diet(Lb group),while dark brown-shelled hens received either a basal diet(Db group)or a basal diet with 10 mg/kg ammonium metavanadate(Dbv group)for 20 d.In Exp.2,light brown-shelled hens received either a basal diet(Lbc group)or a basal diet supplemented with 200 mg/kg resveratrol(Lbr group)for 12 weeks.Results Compared to the Db group,eggshell L*values increased,and PpIX concentrations in both eggshell and uterus decreased in Dbv and Lb groups.These groups also showed oxidative stress,as indicated by reduced hepatic T-SOD and CAT activities.Uterine redox status changes were further confirmed by increased T-AOC level(Dbv)and reduced CAT gene expression(Lb).These redox disturbances led to reduced expression of ND4 and COX1 mt DNA,decreased ATP production and CS activity,along with upregulation of IR,PI3K,HK,and PK gene expression,reflecting altered mitochondrial energy metabolism.Notably,the SIRT1/PGC-1αsignaling cascade and its downstream target ALAS1 were significantly downregulated at both mRNA and protein levels in Dbv and Lb groups.Compared to the Lbc group,the Lbr group exhibited higher antioxidant capacity by increasing hepatic CAT activity and uterine T-SOD and GSH-Px activities,and reducing MDA levels.Moreover,the Lbr group restored mitochondrial function and PpIX biosynthesis by upregulating ND4 and COX1 mt DNA,CS and SDHA gene expression,and SIRT1/PGC-1α/ALAS1 signaling,while downregulating LDH activity and the expression of IR and PI3K,thereby alleviating eggshell color fading.Conclusion Oxidative stress induces eggshell depigmentation by impairing mitochondrial function and downregulating the SIRT1/PGC-1α/ALAS1 pathway,leading to reduced PpIX biosynthesis.Specifically,vanadium-induced or endogenous oxidative stress disrupts mitochondrial energy metabolism and suppresses key components of this pathway,while resveratrol alleviates oxidative damage and restores mitochondrial function and ALAS1-driven PpIX synthesis through reactivation of the SIRT1/PGC-1αaxis.
基金financially supported by the National Basic Research Program of China (973 Program) (no. 2012CB215500)the National Key Technology Research and Development Program of China (no. 2015BAG06B00)+1 种基金Major Program of the National Natural Science Foundation of China (no. 61433013)National Natural Science Foundation of China (no. 21206012)
文摘Arc ion plating (AIP) is applied to form Ti/(Ti,Cr)N/CrN multilayer coating on the surface of 316L stainless steel (SS316L) as bipolar plates for proton exchange membrane fuel cells (PEMFCs). The characterizations of the coating are analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Interfacial contact resistance (ICR) between the coated sample and carbon paper is 4.9 m Omega cm(2) under 150 N/cm(2), which is much lower than that of the SS316L substrate. Potentiodynamic and potentiostatic tests are performed in the simulated PEMFC working conditions to investigate the corrosion behaviors of the coated sample. Superior anticorrosion performance is observed for the coated sample, whose corrosion current density is 0.12 mu A/cm(2). Surface morphology results after corrosion tests indicate that the substrate is well protected by the multilayer coating. Performances of the single cell with the multilayer coated SS316L bipolar plate are improved significantly compared with that of the cell with the uncoated SS316L bipolar plate, presenting a great potential for PEMFC application. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.41702067 and 41602067)National Key Research and Development Program of China(Grant No.2018YFC0603603)+1 种基金Natural Science Foundation of Guangdong Province(Grant No.2017A0303113246)the Fundamental Research Funds for the Central Universities(171gpy63 and 181gpy25)
文摘Colloform pyrite with core-rim texture is commonly deposited in carbonate platforms associated with the sulfide ores such as the Caixiashan Pb-Zn deposit.However,the genesis of colloform pyrite in Pb-Zn deposits,its growth controls and their geological implication are insufficiently understood.Integration of in-situ trace element and SIMS sulfur isotopes has revealed geochemical variations among these pyrite layers.These colloform pyrite occur as residual phases of core-rim aggregates,the cores are made up of very fine-grained anhedral pyrite particles,with some rims being made up of fine-grained and poorlycrystallized pyrite,while the other rims were featured with euhedral cubic pyrite.which are cemented by fine-grained calcite and/or dolomite with minor quartz.Sulfur isotope analysis shows that some wellpreserved rims have negative δ^34 S values(-28.12‰to-0.49‰),whereas most of the cores and rims have positive δ^34 S values(>0 to+44.28‰;peak at+14.91‰).Integrating with the methane and sulfate were observed in previous fluid inclusion study,we suggest that the 34 S depleted rims were initially formed by bacteria sulfate reduction(BSR),whereas the positive δ^34 S values were resulted from the sulfate reduction driven by anaerobic methane oxidation(AOM).The well-developed authigenic pyrite and calcite may also support the reaction of AOM.Combined with petrographic observations,trace element composition of the colloform pyrite reveals the incorporation and precipitation behavior of those high abundance elements in the pyrite:Pb and Zn were present as mineral inclusion and likely precipitated before Fe,as supported by the time-resolved Pb-Zn signal spikes in most of the analyzed pyrite grains.Other metals,such as Hg,Co and Ni,may have migrated as chloride complexes and entered the pyrite lattice.Arsenic and Sb,generally influenced by complex-forming reactions rather than substitution ones,could also enter the pyrite lattice,or slightly predate the precipitation of colloform pyrite as mineral inclusions,which are controlled by their hydrolysis constant in the ore fluids.The colloform pyrite may have grown inward from the rims.The successive BSR reaction process would enrich H^32/2S in the overlying water column but reduce the metal content,the nucleation of these pyrite rims was featured by strongly negative sulfur isotopes.The following AOM process should be activated by deformation like the turbidity sediment of the mudstone as the sulfide deposition are associated with fault activities that caused the emission of methane migration upward and simultaneously replenishing the metal in the column.The higher AOM reaction rate and the higher metal supply(not only Fe.but with minor other metals such as Pb and Zn) caused by sediment movement enhanced the metal concentration within the pyrite lattice.
基金supported by the National Natural Science Foundation of China (Grant Nos. 81671015, 81571815, 81470717)Beijing Municipal Science and Technology Commission (Grant No. Z171100001017128)
文摘Collagen is the building component of temporomandibular joint(TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However,the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague–Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm^(-1)/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs,indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs.
基金financially supported by the State Key Lab of Advanced Metals and Materials of China(Grant No.2020-Z18)National Natural Science Foundation of China(Grant No.52071014)Fundamental Research Funds for the Central Universities(No.FRF-MP-20-51,FRF-BD-20-28A2).
文摘Hydrogen embrittlement behavior, micro-deformation, and crack propagation mechanism of CoCrFeNiMn high-entropy alloy (HEA) fabricated by laser powder bed fusion (LPBF) under different parameters were investigated by slow strain rate tensile tests (at room temperature) with/without electrochemical hydrogen pre-charging. The LPBF CoCrFeNiMn HEA shows excellent resistance to hydrogen embrittlement. Unsuitable LPBF parameters are accompanied by many microcracks and holes, resulting in a slight decrease in the hydrogen embrittlement resistance of the material. The electron backscatter diffraction (EBSD), electron channeling contrast image (ECCI) techniques, and transmission electron microscope (TEM) were carried out to research the main influencing factors of hydrogen on the deformation mechanism and crack propagation. Compared with un-charged samples, a larger number of deformation twins (DTs) appear in the deformation process of hydrogen-charged LPBF CoCrFeNiMn, attributing to the reduction of stacking fault energy (SFE) due to the ingress of hydrogen. The nano DTs and crossing twin system contribute to the extra work hardening, and a strain hardening platform is observed for all hydrogen-charged samples, resulting in the increase of strain hardening rate or the mitigation of the loss of strain hardening. Although unsuitable process parameters will trigger fabrication defects and reduce mechanical properties, the cellular structure can bring a hydrogen-induced strain hardening platform for LPBF CoCrFeNiMn to reduce the damage caused by hydrogen embrittlement.
基金supported by the Southwest University of Science and Technology(No.22zx7168)the Sichuan Science and Technology Program(No.2020YFG0186)。
文摘Low desulfurization efficiency impedes the wide application of dry desulfurization technology,which is a low-cost and simple process,and one significant solution is the development and manufacture of high-performance desulfurizers.In this study,firstly,a steam jet mill was used to digest quicklime;then,we utilized numerical simulation to study the flow field distribution and analyze the driving factors of quicklime digestion;and lastly,the desulfurization performance of the desulfurizer was evaluated under different relative humidities.The results show that the desulfurizer prepared via the steam jet mill had better apparent activity than traditional desulfurizers.Also,the entire jet flow field of the steam jet mill is in a supersonic and highly turbulent flow state,with high crushing intensity and good particle acceleration performance.Sufficient contact with the nascent surface maximizes the formation of slaked lime.The experiments demonstrated that the operating time with 100%desulfurization efficiency and the“break-through”time for the desulfurizer prepared via the steam jet mill is longer than that of traditional desulfurizers,and has significant advantages,especially at low flue gas relative humidity.Compared with traditional desulfurizers,the desulfurizer prepared via steam jet mill expands the range of acceptable flue gas temperature,and the failure temperature is 1.625 times that of traditional desulfurizers.This work breaks through the technical bottleneck of low dry desulfurization efficiency,which is an important step in pushing forward the application of dry desulfurization.
文摘Effects of nucleation sites and diffusivity enhancement of chromium on reverted transformation of AISI 304 stainless steel during annealing process were investigated.Dynamics calculation revealed that the reverted transformation of strain-inducedα’-martensite→γaustenite could were closely associated with active nucleation sites and diffusivity enhancement of chromium in nanocrystallineα’-martensite.The experimental data and the results were in accordance with 2-grain austenite/α’-martensite junctions calculated theoretically,which could result from high chromium diffusion rate in nanocrystallineα’-martensite.In addition,low temperature is not conducive to reversed transformation,while high temperature and long annealing time will lead to inhomogeneous grain size distribution.
文摘Future education is highly integrated with technology and highly open education.The rapid development of information technology urges education to embrace the change brought by technology enthusiastically.The alienation of thinking under technology package must not be ignored and guard against the tendency of"technology determinism".Therefore,this paper interprets the irreplaceable life education from three angles:the reform trend,the basic pattern and the practice construction,and makes full use of technology to restore the meaning of life at the same time.
基金supported by the National Natural Science Foundation of China Nos.82370983,81671015(X.W.),82230030(Y.L.),82101043(S.C.)and 82370922(Y.F.)Beijing International Science and Technology Cooperation Project No.Z221100002722003(Y.L.)+4 种基金Beijing Natural Science Foundation Nos.L234017,JL23002(Y.L.),No.7242282(S.C.)and 7232217(Y.G.)Clinical Medicine Plus X-Young Scholars Project of Peking University No.PKU2024LCXQ039(Y.L.)National Program for Multidisciplinary Cooperative Treatment on Major Diseases No.PKUSSNMP-202013(X.W.)Hygiene and Health Development Scientific Research Fostering Plan of Haidian District Beijing No.HP2023-12-509001(J.Z.)Young Clinical Research Fund of the Chinese Stomatological Association No.CSA-02022-03(J.Z.).
文摘Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.
基金financially supported by the National Natural Science Foundation of China(No.52401161)the Natural Science Foundation of Sichuan Province for Young Scholars(No.24NSFSC6582)+3 种基金the Postdoctoral Fellowship Program of CPSF(No.GZC20231761)the National Natural Science Foundation of China(No.52271249)the Key Research and Development Program of Shaanxi(No.2023-YBGY-488)the Xi'an Talent Plan(No.XAYC240016).
文摘Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic modulus makes Elinvar alloys highly desirable in precision-control applications,including aerospace,electronics,and optical instruments.Currently,most of the used and studied Elinvar alloys are ferromagnetic alloys(FeNi and Fe-Pt)and antiferromagnetic alloys(Fe-Mn and γ-MnCu)[2–4].The Elinvar effect in these alloys typically originates from magnetostriction or magnetoelastic effects,which are magnetic fieldor magnetic transition-dependent[5].Consequently,these Elinvar alloys cannot function properly in the presence of a magnetic field owing to their Elinvar effect being closely tied to magnetic phase transition.Therefore,developing non-magneticdependent Elinvar alloys is highly essential to widen their practical applications.
基金supported by the National Natural Science Foundation of China(grants 81970910 and 82370931)Jiangsu Province Capability Improvement Project through Science,Technology and Education-Jiangsu Provincial Research Hospital Cultivation Unit(YJXYYJSDW4)Jiangsu Provincial Medical Innovation Center(CXZX202227).
文摘Plp1-lineage Schwann cells(SCs)of peripheral nerve play a critical role in vascular remodeling and osteogenic differentiation during the early stage of bone healing,and the abnormal plasticity of SCs would jeopardize the bone regeneration.However,how Plp1-lineage cells respond to injury and initiate the vascularized osteogenesis remains incompletely understood.Here,by employing single-cell transcriptional profiling combined with lineage-specific tracing models,we uncover that Plp1-lineage cells undergoing injury-induced glia-to-MSCs transition contributed to osteogenesis and revascularization in the initial stage of bone injury.Importantly,our data demonstrated that the Sonic hedgehog(Shh)signaling was responsible for the transition process initiation,which was strongly activated by c-Jun/SIRT6/BAF170 complex-driven Shh enhancers.Collectively,these findings depict an injuryspecific niche signal-mediated Plp1-lineage cells transition towards Gli1+MSCs and may be instructive for approaches to promote bone regeneration during aging or other bone diseases.
基金supported by funding from the NSFC(42030306 and 41672216)the National Key R&D Program of China(2016YFC0600102-03).
文摘The largest Tan-Lu active fault system in northeastern Asia,spans approximately 3500 km in length and varies in width from 10 km to 200 km.In 1668,an earthquake with a magnitude of 8.5 occurred in Tancheng,causing the loss of over 50000 lives.To constrain the timing and process of the Tan-Lu fault system on eastern Asian margin,this study presents the field mapping,thin section observation,geochronology,and microanalysis of Weiyuanpu-Yehe ductile shear zone(WYSZ)of the northern Tan-Lu fault system.Kinematic indicators and microstructures suggest a sense of sinistral strike-slip.The deformation temperature of the mylonite is mediate to high based on the quartz deformation,c-axis fabrics.The differential stress of the shear zone is 20‒40 MPa using quartz paleopiezometry.The dikes within the shear zone yielded zircon U-Pb ages of 165‒163 Ma.However,due to the ambiguous geological relationship between the dikes and shear zone,additional geochronology is warranted.Since the Mesozoic era,based on the exposure of mylonite and dikes,the upper crust has been extensively eroded,exposing the ductile shear zone.Moreover,the understanding of the geometry and process of pre-existing structures has fundamental implications for predicating the potential earthquakes for the Tan-Lu fault system.
基金supported by the Key R&D Program of Zhejiang(No.KZ7240079).
文摘Metastable β-Ti alloys exhibiting twinning-induced plasticity (TWIP) and transformation-induced plasticity (TRIP) generally have excellent ductility, but typically at the expense of relatively low yield strengths which has restricted their widespread use. Our work shows that interstitial oxygen can be employed to regulate β phase stability to significantly enhance both strength and ductility of TWIP/TRIP alloys. For a Ti-32Nb wt.% base alloy, inclusion of 0.3 wt.% O enhanced ductility by more than 140 %, reaching up to 54 % strain, and improved the tensile yield strength by over 95 % to 632 MPa. Compared to other common engineering alloys such as Ti-45Nb, elongation was increased by 29 %, and the yield strength increased by 182 MPa, respectively. Here, we elucidate on impacts of oxygen doping on TWIP/TRIP behaviors in the Ti-32Nb alloy. We reveal that oxygen regulates the critical stress for martensitic transformation, twinning, and dislocation slip. At lower oxygen doping concentrations (≤0.3 wt.% O), multi-stage martensitic transformation and martensitic twinning resulted in high ductility. In higher oxygen content alloys (≥0.5 wt.% O), deformation occurred initially via twinning, while strain induced martensite was subsequently induced in retained β phase regions. Oxygen concentrations control the deformation mechanisms, providing a flexible means to synergistically balance an alloy's strength and ductility. The use of oxygen to enhance stability of the β phase and regulate deformation behaviors is a promising new approach for creating high-performance TWIP/TRIP metastable β-Ti alloys with outstanding mechanical properties.
