BACKGROUND Long noncoding RNA(lncRNA)ZNFX1-AS1(ZFAS1)is a newly discovered lncRNA,but its diagnostic value in gastric cancer is unclear.AIM To investigate the potential role of ZFAS1 in gastric cancer and to evaluate ...BACKGROUND Long noncoding RNA(lncRNA)ZNFX1-AS1(ZFAS1)is a newly discovered lncRNA,but its diagnostic value in gastric cancer is unclear.AIM To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening.METHODS Quantitative real-time polymerase chain reaction(qRT-PCR)was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients,gastric stromal tumor patients,gastritis or gastric ulcer patients,and healthy controls.Correlations between ZFAS1 expression and clinicopathological features were analyzed.The biological effects of ZFAS1 on the proliferation,migration,and invasion of gastric cancer cells were studied by MTT,colony formation,and transwell migration assays.The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR.The relationship between ZFAS1 and tumorigenesis was demonstrated using in vivo tumor formation assays.RESULTS The plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups.Increased expression of ZFAS1 was significantly associated with lymph node metastasis,advanced TNM stage,and poor prognosis.ZFAS1 regulated the proliferation,migration,and invasion of gastric cancer cells and regulated the growth of gastric cancer cells in vivo.LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells.CONCLUSION LncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression,suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer.展开更多
Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is ...Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is known about molecular mechanisms underlying anther cell elongation.Here,we found that properly activated cell elongation regulators at transcriptional levels in loss-of-function ZmMs33 mutant(ms33-6038)anthers failed to promote maize anther elongation.ZmMs33 deficiency disrupted metabolic homeostasis mainly by inhibiting both photosynthesis in anther endothecium and lipid accumulation in anther tapetum.Importantly,ms33-6038 anthers displayed ectopic,premature and excessive secondary cell wall thickening in anther middle layer,which constrained cell elongation structurally and blocked nutrient flows across different anther wall layers.The metabolic disorder was only found in ms33-6038 mutant rather than several representative male-sterility lines at transcriptional and post-translational levels.Collectively,the disordered metabolisms and blocked nutrient flows defeated the activated cell elongation regulators,and finally inhibited anther elongation and growth with a unique‘‘idling effect”in ms33-6038 mutant.展开更多
针对涂层提高旋耕刀耐磨性但会增加作业功耗的问题,该研究通过仿真与试验探究了不同厚度涂层对旋耕刀功耗的影响规律,并优选了涂层厚度。通过仿真模拟旋耕刀作业过程,得到旋耕刀易磨损位置和涂层厚度对旋耕刀功耗的影响规律。采用等离...针对涂层提高旋耕刀耐磨性但会增加作业功耗的问题,该研究通过仿真与试验探究了不同厚度涂层对旋耕刀功耗的影响规律,并优选了涂层厚度。通过仿真模拟旋耕刀作业过程,得到旋耕刀易磨损位置和涂层厚度对旋耕刀功耗的影响规律。采用等离子堆焊技术制备1、1.5和2 mm 3种厚度耐磨涂层,并进行田间功耗测试。田间试验结果发现,随旋耕刀涂层厚度由0增至1、1.5、2 mm,平均功耗分别增加了12.4%、17.3%和26.8%,旋耕刀功耗随涂层厚度变化趋势与仿真试验一致,误差范围在0.17%~6.77%之间,验证了仿真模型的准确性,并根据旋耕作业仿真过程分析了涂层导致旋耕刀功耗增长的原因。基于成本分析与涂层耐磨性及其对功耗的影响,确定旋耕刀涂层强化最优厚度为1.25 mm。该研究得到了涂层对旋耕刀功耗影响机制,提出了综合成本、功耗与耐磨性选择涂层厚度的方法,可为旋耕刀涂层强化工艺厚度选择提供理论基础。展开更多
基金Supported by Beijing Natural Science Foundation,No.7172225
文摘BACKGROUND Long noncoding RNA(lncRNA)ZNFX1-AS1(ZFAS1)is a newly discovered lncRNA,but its diagnostic value in gastric cancer is unclear.AIM To investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening.METHODS Quantitative real-time polymerase chain reaction(qRT-PCR)was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients,gastric stromal tumor patients,gastritis or gastric ulcer patients,and healthy controls.Correlations between ZFAS1 expression and clinicopathological features were analyzed.The biological effects of ZFAS1 on the proliferation,migration,and invasion of gastric cancer cells were studied by MTT,colony formation,and transwell migration assays.The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR.The relationship between ZFAS1 and tumorigenesis was demonstrated using in vivo tumor formation assays.RESULTS The plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups.Increased expression of ZFAS1 was significantly associated with lymph node metastasis,advanced TNM stage,and poor prognosis.ZFAS1 regulated the proliferation,migration,and invasion of gastric cancer cells and regulated the growth of gastric cancer cells in vivo.LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells.CONCLUSION LncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression,suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer.
基金funded by the National Key Research and Development Program of China(2021YFF1000302)Fundamental Research Funds for the Central Universities of China(06500136)the Interdisciplinary Research Project for Young Teachers of USTB(Fundamental Research Funds for the Central Universities)(FRF-IDRY-20-038).
文摘Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is known about molecular mechanisms underlying anther cell elongation.Here,we found that properly activated cell elongation regulators at transcriptional levels in loss-of-function ZmMs33 mutant(ms33-6038)anthers failed to promote maize anther elongation.ZmMs33 deficiency disrupted metabolic homeostasis mainly by inhibiting both photosynthesis in anther endothecium and lipid accumulation in anther tapetum.Importantly,ms33-6038 anthers displayed ectopic,premature and excessive secondary cell wall thickening in anther middle layer,which constrained cell elongation structurally and blocked nutrient flows across different anther wall layers.The metabolic disorder was only found in ms33-6038 mutant rather than several representative male-sterility lines at transcriptional and post-translational levels.Collectively,the disordered metabolisms and blocked nutrient flows defeated the activated cell elongation regulators,and finally inhibited anther elongation and growth with a unique‘‘idling effect”in ms33-6038 mutant.
文摘针对涂层提高旋耕刀耐磨性但会增加作业功耗的问题,该研究通过仿真与试验探究了不同厚度涂层对旋耕刀功耗的影响规律,并优选了涂层厚度。通过仿真模拟旋耕刀作业过程,得到旋耕刀易磨损位置和涂层厚度对旋耕刀功耗的影响规律。采用等离子堆焊技术制备1、1.5和2 mm 3种厚度耐磨涂层,并进行田间功耗测试。田间试验结果发现,随旋耕刀涂层厚度由0增至1、1.5、2 mm,平均功耗分别增加了12.4%、17.3%和26.8%,旋耕刀功耗随涂层厚度变化趋势与仿真试验一致,误差范围在0.17%~6.77%之间,验证了仿真模型的准确性,并根据旋耕作业仿真过程分析了涂层导致旋耕刀功耗增长的原因。基于成本分析与涂层耐磨性及其对功耗的影响,确定旋耕刀涂层强化最优厚度为1.25 mm。该研究得到了涂层对旋耕刀功耗影响机制,提出了综合成本、功耗与耐磨性选择涂层厚度的方法,可为旋耕刀涂层强化工艺厚度选择提供理论基础。
