The Beijing-Tianjin-Hebei Plain is among the regions in China that feature the largest scale and the fastest growth in medium and deep geothermal heating.Based on tests of 82 geothermal fluid samples from 7 geothermal...The Beijing-Tianjin-Hebei Plain is among the regions in China that feature the largest scale and the fastest growth in medium and deep geothermal heating.Based on tests of 82 geothermal fluid samples from 7 geothermal fields in Hebei Province,2 geothermal fields in Henan Province,and 2 geothermal fields in Shandong Province,and combined with previous studies on the chemical characteristics of karst geothermal water in Beijing and Tianjin,this paper systematically analyzes the migration characteristics of geothermal fluids in karst geothermal reservoirs within the Beijing-Tianjin-Hebei Plain.The hydrochemical characteristics of karst geothermal water in the research areas exhibit certain differences.The geothermal water in Hebei is more mature than that in its neighboring provinces.The distribution of total dissolved solids(TDS)and strontium elements in the area is characterized by being low in the north and south and high in the middle,suggesting that the overall flow direction of geothermal fluid is from the north and south towards the middle.Combined with the groundwater flow field and the changing trend of the hydrochemical characteristics of geothermal wells along the geological section,a geothermal water migration model has been established.The geothermal fluids originating from Taihang Mountain,Yanshan Mountain,and Western Shandong Mountain enter the basin and continue to migrate towards the central part of the basin along water-conducting faults.However,the migration characteristics of geothermal fluids with the same supply direction are not identical.The geothermal fluids from Taihang Mountain are cut off by the Niudong Fault in the north and terminate in the central uplift belt of the Jizhong Depression,while in the south,they enter the east of the Jizhong Depression relatively quickly along the Hengshui Conversion Belt.The geothermal fluids from Yanshan Mountain migrate into the basin along the Cangdong Fault,yet this fault also disrupts the hydraulic connection between the tectonic units.Considering the effective dynamic conditions,it is recommended to further expand the scale of the scientific development and utilization of geothermal energy in the geothermal water catchment areas around Xiongxian County and southwest Cangzhou City.展开更多
ObjectiveTo explore the clinical effectiveness of xingnao kaiqiao (regaining consciousness and opening orifice) acupuncture therapy for patients with acute ischemic stroke, and establish a prediction model for prognos...ObjectiveTo explore the clinical effectiveness of xingnao kaiqiao (regaining consciousness and opening orifice) acupuncture therapy for patients with acute ischemic stroke, and establish a prediction model for prognosis for the target population through Logistic regression analysis.MethodsNinety-six patients with acute ischemic stroke were randomly assigned to either an ozone therapy group or an ozone therapy plus xingnao kaiqiao acupuncture group (OA group) with a 1:1 ratio. The patients in the ozone group was treated with ozone therapy, and those in the OA group were treated with acupuncture at Nèiguān (内关PC6), Shuĭgōu (水沟GV26), Sānyīnjiāo (三阴交SP6), lower Jíquán (下极泉Extra, lower HT1), Wĕizhōng (委中BL40) and Chĭzé (尺泽LU5) on the basis of the treatment as the ozone therapy group. The treatment was given once daily for 7 days as a session and 3 consecutive sessions were required. Before treatment and 48 h after treatment, the serological indicators (interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α] and hypersensitive C-reactive protein [hs-CRP]) were tested. Using the National Institute of Health stroke scale (NIHSS) score and Montreal cognitive assessment (MoCA) score, the neurological and cognitive functions of patients were assessed. The clinical efficacy was evaluated with the modified Rankin scale, and the incidence of adverse effects was analyzed statistically in two groups. The multivariate Logistic regression analysis was adopted to detect the risk factors of the prognosis in patients and the nomographic prediction model was established.Results(1) clinical therapeutic effect: after treatment, the levels of IL-6, TNF-α and Hs-CRP in the OA group were significantly lower than that in the ozone group (all P < 0.05). After treatment, compared with the ozone therapy group, NIHSS score was lower and the scores of BI and MoCA were significantly higher in the OA group (all P < 0.05). The total effective rate of the OA group was 91.67%, higher than that of the ozone therapy group (62.50%, P < 0.05). The incidence of adverse effects in the OA group was lower when compared with that in the ozone group (P < 0.05);(2) establishment of prediction model for prognosis: age, history of diabetes, cardiogenic cerebral embolism, high density lipoprotein-cholesterol (HDL-C), white blood cell (WBC) and treatment methods were independent risk factors of the unfavorable prognosis for the patients with acute stroke. The established model had sound accuracy for prediction of prognosis.ConclusionXingnao kaiqiao acupuncture was effective on acute ischemic stroke, and the prognosis of this therapy was increased significantly when combined with the ozone therapy. The preventive measures should be set up for patients with risk factors, e.g. advanced age, diabetes, cardiogenic cerebral embolism, low HDL-C and abnormal WBC, in order to further reduce the risk of unfavorable prognosis in patients with acute stroke.展开更多
Interleukin 17(IL-17)and its main producer,T cell receptorγδcells,have neurotoxic effects in the pathogenesis of intracerebral hemorrhage(ICH),aggravating brain injuries.To investigate the correlation between IL-17 ...Interleukin 17(IL-17)and its main producer,T cell receptorγδcells,have neurotoxic effects in the pathogenesis of intracerebral hemorrhage(ICH),aggravating brain injuries.To investigate the correlation between IL-17 and ICH,we dynamically screened serum IL-17 concentrations using enzyme-linked immunosorbent assay and explored the clinical values of IL-17 in ICH patients.There was a significant negative correlation between serum IL-17 level and neurological recovery status in ICH patients(r=–0.498,P<0.01).To study the neurotoxic role of IL-17,C57 BL/6 mice were used to establish an ICH model by injecting autologous blood into the caudate nucleus.Subsequently,the mice were treated with mouse neural stem cells(NSCs)and/or IL-17 neutralizing antibody for 72 hours.Flow cytometry,brain water content detection,Nissl staining,and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling results indicated that NSC transplantation significantly reduced IL-17 expression in peri-hematoma tissue,but there was no difference in T cell receptorγδcells.Compared with the ICH group,there were fewer apoptotic bodies and more Nissl bodies in the ICH+NSC group and the ICH+NSC+IL-17 group.To investigate the potential effect of IL-17 on directional differentiation of NSCs,we cultured mouse NSCs(NE-4 C)alone or co-cultured them with T cell receptorγδcells,which were isolated from mouse peripheral blood mononuclear cells,for 7 days.The results of western blot assays revealed that IL-17 secreted by T cell receptorγδcells reduced the differentiation of NSCs into astrocytes and neurons,while IL-17 neutralization relieved the inhibition of directional differentiation into astrocytes rather than neurons.In conclusion,serum IL-17 levels were elevated in the early stage of ICH and were negatively correlated with outcome in ICH patients.Animal experiments and cytological investigations therefore demonstrated that IL-17 probably has neurotoxic roles in ICH because of its inhibitory effects on the directional differentiation of NSCs.The application of IL-17 neutralizing antibody may promote the directional differentiation of NSCs into astrocytes.This study was approved by the Clinical Research Ethics Committee of Anhui Medical University of China(For human study:Approval No.20170135)in December 2016.All animal handling and experimentation were reviewed and approved by the Institutional Animal Care and Use Committee of Anhui Medical University(approval No.20180248)in December 2017.展开更多
Dexamethasone has been widely used after various neurosurgical procedures due to its anti-inflammatory property and the abilities to restore vascular permeability,inhibit free radicals,and reduce cerebrospinal fluid p...Dexamethasone has been widely used after various neurosurgical procedures due to its anti-inflammatory property and the abilities to restore vascular permeability,inhibit free radicals,and reduce cerebrospinal fluid production.According to the latest guidelines for the treatment of traumatic brain injury in the United States,high-dose glucocorticoids cause neurological damage.To investigate the reason why high-dose glucocorticoids after traumatic brain injury exhibit harmful effect,rat controlled cortical impact models of traumatic brain injury were established.At 1 hour and 2 days after surgery,rat models were intraperitoneally administered dexamethasone 10 mg/kg.The results revealed that 31 proteins were significantly upregulated and 12 proteins were significantly downregulated in rat models of traumatic brain injury after dexamethasone treatment.The Ingenuity Pathway Analysis results showed that differentially expressed proteins were enriched in the mitochondrial dysfunction pathway and synaptogenesis signaling pathway.Western blot analysis and immunohistochemistry results showed that Ndufv2,Maob and Gria3 expression and positive cell count in the dexamethasone-treated group were significantly greater than those in the model group.These findings suggest that dexamethasone may promote a compensatory increase in complex I subunits(Ndufs2 and Ndufv2),increase the expression of mitochondrial enzyme Maob,and upregulate synaptic-transmission-related protein Gria3.These changes may be caused by nerve injury after traumatic brain injury treatment by dexamethasone.The study was approved by Institutional Ethics Committee of Beijing Neurosurgical Institute(approval No.201802001)on June 6,2018.展开更多
Permian volcanic rocks are widely distributed in the Turpan-Hami Basin, which is part of the Central Asian orogenic belt. Here we present SHRIMP zircon data for the rhyolite in Well Baocan 1, one of the deepest wells ...Permian volcanic rocks are widely distributed in the Turpan-Hami Basin, which is part of the Central Asian orogenic belt. Here we present SHRIMP zircon data for the rhyolite in Well Baocan 1, one of the deepest wells in the basin. The 283.9±2.7 Ma reported in our study provides the best precise age determination for the Yierxitu Formation, the oldest Permian layer of Hami Depression, one of the three substructural units of the Turpan-Hami Basin, and a potential hydrocarbon reservoir in this basin. Our data refines earlier imprecise 39^Ar-40^Ar ages and shows that the volcanic rocks both inside the Turpan-Hami Basin and along its margin are almost coeval. We delineate a collisional orogenesis, and the new age of 283.9±2.7 Ma may limit the latest time of the collision orogenesis.展开更多
Under the background of big data,Informatization plays an important role in the development of the engineering cost industry.The rapid development of the industry and the increasing complexity of construction projects...Under the background of big data,Informatization plays an important role in the development of the engineering cost industry.The rapid development of the industry and the increasing complexity of construction projects require higher standards of informatization.The current information processing methods and models have been difficultly to meet new requirements.Based on this,this study deeply analyzes the key factors that impede the informatization of engineering cost development,and tries to find corresponding solutions through theoretical analysis and empirical research to break these constraints.This will play a guiding role in the development of informatization in China's engineering cost industry.展开更多
Finding suitable strategies to effectively enhance the optical properties of materials are the goal being pursued by researchers.Herein,cation-anion synergetic interactions strategy was proposed to develop two novel o...Finding suitable strategies to effectively enhance the optical properties of materials are the goal being pursued by researchers.Herein,cation-anion synergetic interactions strategy was proposed to develop two novel organic-inorganic hybrid antimony-based optical materials,(C_(3)H_(5)N_(2))Sb F_(2)SO_(4)(I)and(C_(5)H_(6)N)Sb F_(2)SO_(4)(Ⅱ),which were obtained by introducing Sb^(3+)cation containing stereochemically active lone-pair(SCALP)and organicπ-conjugated cations into sulphate system.The synergistic interactions of the organicπ-conjugated cations,the inorganic[SbO_(2)F_(2)]^(3-)seesaw anions and the[SO_(4)]^(2-)distorted tetrahedra anions make their ultraviolet(UV)absorption edges approach 297 and 283 nm,respectively,and raise their birefringence up to 0.193@546 nm and 0.179@546 nm,respectively.Interestingly,although the two compounds have the same stoichiometric ratio and similar one-dimensional(1D)chain structure,they show opposite macroscopic symmetry,where the NCS compound(Ⅱ)exhibits a large secondharmonic generation(SHG)response(1.6 times that of KH_(2)PO_(4)).The two reported compounds are found to be promising UV optical materials in the experimental tests.展开更多
To the Editor:Traumatic brain injury(TBI)is a life-threatening condition and global public health concern in both developed and developing countries.[1]During the last few decades,the morbidity and mortality associate...To the Editor:Traumatic brain injury(TBI)is a life-threatening condition and global public health concern in both developed and developing countries.[1]During the last few decades,the morbidity and mortality associated with TBI have decreased.However,the precise mechanisms that contribute to the outcomes remain unclear.Recently,researchers have become aware that owing to the brain–gut axis interaction,the central nervous system is closely related to the gut microbiota.[2]Many studies have correlated the gut microbiota and its metabolites with autism,depression,stroke,and Parkinson’s disease.[3]Whether changes in the gut microbial composition affect TBI remains largely unknown.Metabolomics,a method focuses on deriving a comprehensive analysis of low-molecular-weight endogenous metabolites in biofluids or tissues and has been used to explore metabolic changes in a variety of diseases.However,the relationship between the metabolome and TBI outcomes or progression and the relationship between the gut microbiome and TBI remain unclear.Hence,we compared the gut microbiome and serum metabolome of patients with TBI and healthy individuals to identify specific bacterial and serum metabolome differences.展开更多
Recent evidence has highlighted immune checkpoint inhibitors as among the most promising immunotherapies for various malignancies.However,a significant proportion of HCC patients exhibit poor responses.Lipid metabolic...Recent evidence has highlighted immune checkpoint inhibitors as among the most promising immunotherapies for various malignancies.However,a significant proportion of HCC patients exhibit poor responses.Lipid metabolic heterogeneity is considered a key driver of cancer progression.However,the role of lipid metabolic reprogramming in HCC immunotherapy resistance remains poorly understood.Herein,we aimed to illuminate the potential relationship between lipid metabolic reprogramming and ICI resistance and provide novel strategies to increase the HCC immunotherapy response.Patients who received PD-1/PD-L1 inhibitors were enrolled.The effect of TACC3 on the tumor microenvironment was validated via single-cell RNA sequencing in HCC-bearing mouse models.Targeted metabolomics was performed to analyze the regulatory role of TACC3 in HCC metabolism.To address HCC immunotherapy resistance,we developed a targeted nucleic acid therapeutic utilizing N-acetylgalactosamine(GalNAc)to conjugate siTACC3.Through clinical cohort analysis,we found that TACC3 was overexpressed in HCC patients with poor response to immunotherapy.Furthermore,we demonstrated that silencing tumor-derived TACC3 optimizes the cytotoxicity of infiltrating CD8^(+)T lymphocytes.Both in vitro and in vivo assays suggested that TACC3 maintains ACSL4-mediated polyunsaturated fatty acid(PUFA)metabolism in HCC cells.Additionally,TACC3 accelerates ACSL4 expression by interacting with LARP1 and PABPC1,which stabilize ACSL4 mRNA.The results of preclinical models demonstrated the satisfactory efficacy of GalNAc-conjugated siTACC3 combined with PD-1 inhibitor therapy for HCC.In summary,tumor-derived TACC3 impairs the tumor-killing activity of CD8^(+)T lymphocytes through PUFA metabolism-associated crosstalk.Targeting TACC3 represents a novel and practicable strategy to augment ICI efficacy against HCC.展开更多
Two-dimensional(2D)MXene materials are promising candidates for the development of heterogeneous materials,yet deciphering structural impacts on their inherent physical properties poses significant challenges.We intro...Two-dimensional(2D)MXene materials are promising candidates for the development of heterogeneous materials,yet deciphering structural impacts on their inherent physical properties poses significant challenges.We introduce structurally regulated 2D Ti_(3)C_(2)nanosheets that were fabricated using high intensity focused ultrasound(HIFU)method.These nanosheets were easily produced in large quantities with a high yield of 94.4%and exhibited excellent photothermal and electrochemical catalytic properties.By utilizing this monolayer H-Ti_(3)C_(2)(HIFU-treated Ti_(3)C_(2)),the heterogeneous integration exhibited promising performance in subsequent applications.By integrating with a hydrogel matrix(H-Ti_(3)C_(2),0.1 wt.%),it demonstrated a photothermal conversion efficiency reaching 43.08%,and the maximum temperature increased by 48.14%under near-infrared(NIR)irradiation.Additionally,these 2D nanosheets were also utilized in fabricating electrochemical sensors to evaluate electrochemical-catalytic capabilities.Notably,it confirmed that the electrocatalytic activity of heterogeneous Au/H-Ti_(3)C_(2)in electrochemical dopamine(DA)detection,and it proved the exhibition of sensitivity(0.012μA/μM),low detection limit(0.15μM),and excellent anti-interference performance and stability.The enhanced activity was ascribed to synergistic effect of Au and the increased number of accessible active sites on the nanosheets.This work not only sheds light on the structure-property relationship of 2D Ti_(3)C_(2)but also broadens the application scope of typical dimensional materials for controlling and producing in biological and sensing applications.展开更多
Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds af...Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds after resection of tumors.In this study,we introduce a bioinspired adhesive hydrogel derived from the skin secretions of Andrias davidianus(SSAD)as a wound dressing for localized drug elution.The hydrogel was loaded with aminoguanidine or doxorubicin,and its controlled drug release and healing-promoting properties were verified in a diabetic rat palatal mucosal defect model and a C57BL/6 mouse melanoma-bearing model,respectively.The results showed that SSAD hydrogels with different pore sizes could release drugs in a controllable manner and accelerate wound healing.Transcriptome analyses of the palatal mucosa suggested that SSAD could significantly upregulate pathways linked to cell adhesion and extracellular matrix deposition and had the ability to recruit keratinocyte stem cells to defect sites.Taken together,these findings indicate that property-controllable SSAD hydrogels could be a promising biofunctional wound dressing for local drug delivery and promotion of wound healing.展开更多
Background Surgical site infection(SSI)is a common complication following craniotomy that increases morbidity,mortality,and medical expenses.The objectives of this study were to determine the relevant risk factors ass...Background Surgical site infection(SSI)is a common complication following craniotomy that increases morbidity,mortality,and medical expenses.The objectives of this study were to determine the relevant risk factors associated with SSI after elective craniotomy for brain tumor and analyse the treatments for SSI.Methods A retrospective nested case-control study was conducted using data from patients who underwent craniotomy for brain tumor resection at the Neurosurgical Oncology Department No.6 of Beijing Tiantan Hospital,Capital Medical University,between January 2019 and December 2021.Risk factors for SSI were determined using multivariate logistic regression analysis.We analyzed microbiological and related treatment data for different SSI types.Results Among 2061 patients who underwent craniotomy for brain tumor,31 had SSI(1.50%).In the multivariate logistic regression analysis,body mass index(BMI)and operative duration were identified as independent risk factors for SSI.The most common microorganism isolated from SSIs was Staphylococcus epidermidis(22.9%),and drug sensitivity results showed that gram-positive bacteria were sensitive to linezolid,vancomycin and tigecycline,whereas gram-negative bacteria were sensitive to meropenem,cefepime and ceftazidime.Six of the seven patients who underwent bone flap removal due to osteomyelitis were infected with gram-negative bacteria.Conclusions BMI and operative duration were identified as independent risk factors for SSI.Diabetes mellitus,previous ratio therapy,type of incision,recurrence tumor and other risk factors were not found to be associated with the occurrence of SSI in this study.展开更多
Direct mixture of Au^3＋ with glutathione （GSH）, which act as both reduction agents and stabilizers, in aqueous solution gave rise to production of gold nanoparticles （Au NPs） with uniform sizes of around 21 nm. T...Direct mixture of Au^3＋ with glutathione （GSH）, which act as both reduction agents and stabilizers, in aqueous solution gave rise to production of gold nanoparticles （Au NPs） with uniform sizes of around 21 nm. The GSH stabilizer Au NPs in solution show immediate aggregation after addition of 1 mol/L NaCI aqueous solution containing Pb^2＋ ions. The Pb^2＋-induced aggregation in Au NP solution is monitored by both colorimetric response and UV-vis spectroscopy. A rather broad linear range （from 0.1 to 30 pmol/L） and low detection limit （0.1 pmol/L） are explored for Au NP sensors used for detection of Pb^2＋ ions. Furthermore, the response of GSH-stabilized Au NPs toward Pb^2＋ ions is specific compared with other possible interferants （Hg^2＋, Mg^2＋, Zn^2＋, Ni^2＋, Cu^2＋, Co^2＋, Ca^2＋, Mn^2＋, Cd^2＋, and Ba^2＋）.展开更多
Zero Poisson’s ratio(ZPR)mechanical metamaterials can yield no transverse displacements when unidi-rectionally compressed,and cylindrical sandwich meta-structures composed of semi re-entrant(SRE)ZPR metamaterials are...Zero Poisson’s ratio(ZPR)mechanical metamaterials can yield no transverse displacements when unidi-rectionally compressed,and cylindrical sandwich meta-structures composed of semi re-entrant(SRE)ZPR metamaterials are thus explored for applications on cylindrical shells of underwater equipment or sub-mersible structures.A group of ZPR unit cells with specified pre-strained wave propagation characteristics and adequate load-bearing capabilities is optimally designed based on the periodic boundary condition(PBC)and Bloch’s Theorem.The sound transmission and pressure-resistant performance of cylindrical sandwich meta-structures comprising the homogeneous and graded SRE ZPR unit cells are then investi-gated.The results show that the designed meta-structures can perfectly yield better vibroacoustic atten-uation behavior within the specified frequency regions corresponding to the pre-strained band gaps and safely bear the hydrostatic pressure equivalent to 1000 m depth with low weight-bulk ratios.In addition,the functionally graded metamaterial core can boost vibroacoustic performance within broader frequency ranges.展开更多
基金support received from the National Major Special Project titled“Exploration and Evaluation of Deep Geothermal Energy in Key Regions”(Project No.2024ZD1003600)the Science and Technology Department of Sinopec(Project No.JP24071).
文摘The Beijing-Tianjin-Hebei Plain is among the regions in China that feature the largest scale and the fastest growth in medium and deep geothermal heating.Based on tests of 82 geothermal fluid samples from 7 geothermal fields in Hebei Province,2 geothermal fields in Henan Province,and 2 geothermal fields in Shandong Province,and combined with previous studies on the chemical characteristics of karst geothermal water in Beijing and Tianjin,this paper systematically analyzes the migration characteristics of geothermal fluids in karst geothermal reservoirs within the Beijing-Tianjin-Hebei Plain.The hydrochemical characteristics of karst geothermal water in the research areas exhibit certain differences.The geothermal water in Hebei is more mature than that in its neighboring provinces.The distribution of total dissolved solids(TDS)and strontium elements in the area is characterized by being low in the north and south and high in the middle,suggesting that the overall flow direction of geothermal fluid is from the north and south towards the middle.Combined with the groundwater flow field and the changing trend of the hydrochemical characteristics of geothermal wells along the geological section,a geothermal water migration model has been established.The geothermal fluids originating from Taihang Mountain,Yanshan Mountain,and Western Shandong Mountain enter the basin and continue to migrate towards the central part of the basin along water-conducting faults.However,the migration characteristics of geothermal fluids with the same supply direction are not identical.The geothermal fluids from Taihang Mountain are cut off by the Niudong Fault in the north and terminate in the central uplift belt of the Jizhong Depression,while in the south,they enter the east of the Jizhong Depression relatively quickly along the Hengshui Conversion Belt.The geothermal fluids from Yanshan Mountain migrate into the basin along the Cangdong Fault,yet this fault also disrupts the hydraulic connection between the tectonic units.Considering the effective dynamic conditions,it is recommended to further expand the scale of the scientific development and utilization of geothermal energy in the geothermal water catchment areas around Xiongxian County and southwest Cangzhou City.
基金Supported by Research on TCM nursing technology promoting rehabilitation of apoplexy patients:YYZX2019063。
文摘ObjectiveTo explore the clinical effectiveness of xingnao kaiqiao (regaining consciousness and opening orifice) acupuncture therapy for patients with acute ischemic stroke, and establish a prediction model for prognosis for the target population through Logistic regression analysis.MethodsNinety-six patients with acute ischemic stroke were randomly assigned to either an ozone therapy group or an ozone therapy plus xingnao kaiqiao acupuncture group (OA group) with a 1:1 ratio. The patients in the ozone group was treated with ozone therapy, and those in the OA group were treated with acupuncture at Nèiguān (内关PC6), Shuĭgōu (水沟GV26), Sānyīnjiāo (三阴交SP6), lower Jíquán (下极泉Extra, lower HT1), Wĕizhōng (委中BL40) and Chĭzé (尺泽LU5) on the basis of the treatment as the ozone therapy group. The treatment was given once daily for 7 days as a session and 3 consecutive sessions were required. Before treatment and 48 h after treatment, the serological indicators (interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α] and hypersensitive C-reactive protein [hs-CRP]) were tested. Using the National Institute of Health stroke scale (NIHSS) score and Montreal cognitive assessment (MoCA) score, the neurological and cognitive functions of patients were assessed. The clinical efficacy was evaluated with the modified Rankin scale, and the incidence of adverse effects was analyzed statistically in two groups. The multivariate Logistic regression analysis was adopted to detect the risk factors of the prognosis in patients and the nomographic prediction model was established.Results(1) clinical therapeutic effect: after treatment, the levels of IL-6, TNF-α and Hs-CRP in the OA group were significantly lower than that in the ozone group (all P < 0.05). After treatment, compared with the ozone therapy group, NIHSS score was lower and the scores of BI and MoCA were significantly higher in the OA group (all P < 0.05). The total effective rate of the OA group was 91.67%, higher than that of the ozone therapy group (62.50%, P < 0.05). The incidence of adverse effects in the OA group was lower when compared with that in the ozone group (P < 0.05);(2) establishment of prediction model for prognosis: age, history of diabetes, cardiogenic cerebral embolism, high density lipoprotein-cholesterol (HDL-C), white blood cell (WBC) and treatment methods were independent risk factors of the unfavorable prognosis for the patients with acute stroke. The established model had sound accuracy for prediction of prognosis.ConclusionXingnao kaiqiao acupuncture was effective on acute ischemic stroke, and the prognosis of this therapy was increased significantly when combined with the ozone therapy. The preventive measures should be set up for patients with risk factors, e.g. advanced age, diabetes, cardiogenic cerebral embolism, low HDL-C and abnormal WBC, in order to further reduce the risk of unfavorable prognosis in patients with acute stroke.
基金supported by the Natural Science Foundation of Anhui Province of China,No.1708085MH211(to HWC)the College Top-notch Talent Foundation of Anhui Province of China,No.KJ2018A0207(to HWC)
文摘Interleukin 17(IL-17)and its main producer,T cell receptorγδcells,have neurotoxic effects in the pathogenesis of intracerebral hemorrhage(ICH),aggravating brain injuries.To investigate the correlation between IL-17 and ICH,we dynamically screened serum IL-17 concentrations using enzyme-linked immunosorbent assay and explored the clinical values of IL-17 in ICH patients.There was a significant negative correlation between serum IL-17 level and neurological recovery status in ICH patients(r=–0.498,P<0.01).To study the neurotoxic role of IL-17,C57 BL/6 mice were used to establish an ICH model by injecting autologous blood into the caudate nucleus.Subsequently,the mice were treated with mouse neural stem cells(NSCs)and/or IL-17 neutralizing antibody for 72 hours.Flow cytometry,brain water content detection,Nissl staining,and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling results indicated that NSC transplantation significantly reduced IL-17 expression in peri-hematoma tissue,but there was no difference in T cell receptorγδcells.Compared with the ICH group,there were fewer apoptotic bodies and more Nissl bodies in the ICH+NSC group and the ICH+NSC+IL-17 group.To investigate the potential effect of IL-17 on directional differentiation of NSCs,we cultured mouse NSCs(NE-4 C)alone or co-cultured them with T cell receptorγδcells,which were isolated from mouse peripheral blood mononuclear cells,for 7 days.The results of western blot assays revealed that IL-17 secreted by T cell receptorγδcells reduced the differentiation of NSCs into astrocytes and neurons,while IL-17 neutralization relieved the inhibition of directional differentiation into astrocytes rather than neurons.In conclusion,serum IL-17 levels were elevated in the early stage of ICH and were negatively correlated with outcome in ICH patients.Animal experiments and cytological investigations therefore demonstrated that IL-17 probably has neurotoxic roles in ICH because of its inhibitory effects on the directional differentiation of NSCs.The application of IL-17 neutralizing antibody may promote the directional differentiation of NSCs into astrocytes.This study was approved by the Clinical Research Ethics Committee of Anhui Medical University of China(For human study:Approval No.20170135)in December 2016.All animal handling and experimentation were reviewed and approved by the Institutional Animal Care and Use Committee of Anhui Medical University(approval No.20180248)in December 2017.
基金This study was supported by the National Natural Science Foundation of China,No.81771327(to BYL)the Platform Construction of Basic Research and Clinical Translation of Nervous System Injury,China,No.PXM2020_026280_000002(to BYL)the Scientific Research and Cultivation Fund of the Beijing Neurosurgical Institute,China,No.2020002(to FN).
文摘Dexamethasone has been widely used after various neurosurgical procedures due to its anti-inflammatory property and the abilities to restore vascular permeability,inhibit free radicals,and reduce cerebrospinal fluid production.According to the latest guidelines for the treatment of traumatic brain injury in the United States,high-dose glucocorticoids cause neurological damage.To investigate the reason why high-dose glucocorticoids after traumatic brain injury exhibit harmful effect,rat controlled cortical impact models of traumatic brain injury were established.At 1 hour and 2 days after surgery,rat models were intraperitoneally administered dexamethasone 10 mg/kg.The results revealed that 31 proteins were significantly upregulated and 12 proteins were significantly downregulated in rat models of traumatic brain injury after dexamethasone treatment.The Ingenuity Pathway Analysis results showed that differentially expressed proteins were enriched in the mitochondrial dysfunction pathway and synaptogenesis signaling pathway.Western blot analysis and immunohistochemistry results showed that Ndufv2,Maob and Gria3 expression and positive cell count in the dexamethasone-treated group were significantly greater than those in the model group.These findings suggest that dexamethasone may promote a compensatory increase in complex I subunits(Ndufs2 and Ndufv2),increase the expression of mitochondrial enzyme Maob,and upregulate synaptic-transmission-related protein Gria3.These changes may be caused by nerve injury after traumatic brain injury treatment by dexamethasone.The study was approved by Institutional Ethics Committee of Beijing Neurosurgical Institute(approval No.201802001)on June 6,2018.
基金supported by the Chinese MOST 973 Program (No. 2009CB219302)
文摘Permian volcanic rocks are widely distributed in the Turpan-Hami Basin, which is part of the Central Asian orogenic belt. Here we present SHRIMP zircon data for the rhyolite in Well Baocan 1, one of the deepest wells in the basin. The 283.9±2.7 Ma reported in our study provides the best precise age determination for the Yierxitu Formation, the oldest Permian layer of Hami Depression, one of the three substructural units of the Turpan-Hami Basin, and a potential hydrocarbon reservoir in this basin. Our data refines earlier imprecise 39^Ar-40^Ar ages and shows that the volcanic rocks both inside the Turpan-Hami Basin and along its margin are almost coeval. We delineate a collisional orogenesis, and the new age of 283.9±2.7 Ma may limit the latest time of the collision orogenesis.
基金Innovation Training Project of Hunan Province(S201911532062).
文摘Under the background of big data,Informatization plays an important role in the development of the engineering cost industry.The rapid development of the industry and the increasing complexity of construction projects require higher standards of informatization.The current information processing methods and models have been difficultly to meet new requirements.Based on this,this study deeply analyzes the key factors that impede the informatization of engineering cost development,and tries to find corresponding solutions through theoretical analysis and empirical research to break these constraints.This will play a guiding role in the development of informatization in China's engineering cost industry.
基金supported by the National Natural Science Foundation of China(Nos.22122106,22071158,21971171,22305166)the Fundamental Research Funds from Sichuan University(No.2021SCUNL101)the Natural Science Foundation of Sichuan Province(No.2023NSFC1066)。
文摘Finding suitable strategies to effectively enhance the optical properties of materials are the goal being pursued by researchers.Herein,cation-anion synergetic interactions strategy was proposed to develop two novel organic-inorganic hybrid antimony-based optical materials,(C_(3)H_(5)N_(2))Sb F_(2)SO_(4)(I)and(C_(5)H_(6)N)Sb F_(2)SO_(4)(Ⅱ),which were obtained by introducing Sb^(3+)cation containing stereochemically active lone-pair(SCALP)and organicπ-conjugated cations into sulphate system.The synergistic interactions of the organicπ-conjugated cations,the inorganic[SbO_(2)F_(2)]^(3-)seesaw anions and the[SO_(4)]^(2-)distorted tetrahedra anions make their ultraviolet(UV)absorption edges approach 297 and 283 nm,respectively,and raise their birefringence up to 0.193@546 nm and 0.179@546 nm,respectively.Interestingly,although the two compounds have the same stoichiometric ratio and similar one-dimensional(1D)chain structure,they show opposite macroscopic symmetry,where the NCS compound(Ⅱ)exhibits a large secondharmonic generation(SHG)response(1.6 times that of KH_(2)PO_(4)).The two reported compounds are found to be promising UV optical materials in the experimental tests.
基金supported by grants from National Natural Science Foundation of China(No.82202438)Clinical and Translational Research Project of Anhui Province(No.202427b10020130)+3 种基金Beijing Postdoctoral Foundation(No.2020-ZZ-008)Returned overseas students from Anhui Province in 2020 Innovation and Entrepreneurship Support Plan Project(No.2020LCX016)Funding of The Anhui Universal Natural Science Foundation(No.KJ2020A0172)Cultivate Funding of the National Natural Science Foundation of China(No.2016KJ12).
文摘To the Editor:Traumatic brain injury(TBI)is a life-threatening condition and global public health concern in both developed and developing countries.[1]During the last few decades,the morbidity and mortality associated with TBI have decreased.However,the precise mechanisms that contribute to the outcomes remain unclear.Recently,researchers have become aware that owing to the brain–gut axis interaction,the central nervous system is closely related to the gut microbiota.[2]Many studies have correlated the gut microbiota and its metabolites with autism,depression,stroke,and Parkinson’s disease.[3]Whether changes in the gut microbial composition affect TBI remains largely unknown.Metabolomics,a method focuses on deriving a comprehensive analysis of low-molecular-weight endogenous metabolites in biofluids or tissues and has been used to explore metabolic changes in a variety of diseases.However,the relationship between the metabolome and TBI outcomes or progression and the relationship between the gut microbiome and TBI remain unclear.Hence,we compared the gut microbiome and serum metabolome of patients with TBI and healthy individuals to identify specific bacterial and serum metabolome differences.
基金supported by the National Natural Science Foundation of China(82430089,82202974)the China Postdoctoral Science Foundation(2024M750533)the Shanghai Anti-Cancer Association(SACA-CY22C10).
文摘Recent evidence has highlighted immune checkpoint inhibitors as among the most promising immunotherapies for various malignancies.However,a significant proportion of HCC patients exhibit poor responses.Lipid metabolic heterogeneity is considered a key driver of cancer progression.However,the role of lipid metabolic reprogramming in HCC immunotherapy resistance remains poorly understood.Herein,we aimed to illuminate the potential relationship between lipid metabolic reprogramming and ICI resistance and provide novel strategies to increase the HCC immunotherapy response.Patients who received PD-1/PD-L1 inhibitors were enrolled.The effect of TACC3 on the tumor microenvironment was validated via single-cell RNA sequencing in HCC-bearing mouse models.Targeted metabolomics was performed to analyze the regulatory role of TACC3 in HCC metabolism.To address HCC immunotherapy resistance,we developed a targeted nucleic acid therapeutic utilizing N-acetylgalactosamine(GalNAc)to conjugate siTACC3.Through clinical cohort analysis,we found that TACC3 was overexpressed in HCC patients with poor response to immunotherapy.Furthermore,we demonstrated that silencing tumor-derived TACC3 optimizes the cytotoxicity of infiltrating CD8^(+)T lymphocytes.Both in vitro and in vivo assays suggested that TACC3 maintains ACSL4-mediated polyunsaturated fatty acid(PUFA)metabolism in HCC cells.Additionally,TACC3 accelerates ACSL4 expression by interacting with LARP1 and PABPC1,which stabilize ACSL4 mRNA.The results of preclinical models demonstrated the satisfactory efficacy of GalNAc-conjugated siTACC3 combined with PD-1 inhibitor therapy for HCC.In summary,tumor-derived TACC3 impairs the tumor-killing activity of CD8^(+)T lymphocytes through PUFA metabolism-associated crosstalk.Targeting TACC3 represents a novel and practicable strategy to augment ICI efficacy against HCC.
基金supported by the Chongqing Municipal Natural Science Foundation(No.CSTB2022NSCQ-MSX0113)the National Natural and Science Foundation of China(Nos.52273287 and 82002256)+2 种基金was partially funded by Natural Science Foundation of Heilongjiang Province of China for Excellent Young Scholars(No.YQ2022E020)the Heilongjiang Touyan Team(No.HITTY-20190036)the Fundamental Research Funds for the Central Universities(No.AUEA5740202424)。
文摘Two-dimensional(2D)MXene materials are promising candidates for the development of heterogeneous materials,yet deciphering structural impacts on their inherent physical properties poses significant challenges.We introduce structurally regulated 2D Ti_(3)C_(2)nanosheets that were fabricated using high intensity focused ultrasound(HIFU)method.These nanosheets were easily produced in large quantities with a high yield of 94.4%and exhibited excellent photothermal and electrochemical catalytic properties.By utilizing this monolayer H-Ti_(3)C_(2)(HIFU-treated Ti_(3)C_(2)),the heterogeneous integration exhibited promising performance in subsequent applications.By integrating with a hydrogel matrix(H-Ti_(3)C_(2),0.1 wt.%),it demonstrated a photothermal conversion efficiency reaching 43.08%,and the maximum temperature increased by 48.14%under near-infrared(NIR)irradiation.Additionally,these 2D nanosheets were also utilized in fabricating electrochemical sensors to evaluate electrochemical-catalytic capabilities.Notably,it confirmed that the electrocatalytic activity of heterogeneous Au/H-Ti_(3)C_(2)in electrochemical dopamine(DA)detection,and it proved the exhibition of sensitivity(0.012μA/μM),low detection limit(0.15μM),and excellent anti-interference performance and stability.The enhanced activity was ascribed to synergistic effect of Au and the increased number of accessible active sites on the nanosheets.This work not only sheds light on the structure-property relationship of 2D Ti_(3)C_(2)but also broadens the application scope of typical dimensional materials for controlling and producing in biological and sensing applications.
基金supported by the National Natural Science Foundation of China(32070826,81801929)the Chinese Postdoctoral Science Foundation(2019M650239,2020T130762)+4 种基金the Chongqing Research Program of Basic Research and Frontier Technology(cstc2018jcyjAX0807)the Innovative Talents Project of Chongqing Postdoctoral Foundation(YRSB(2019)298)the Chongqing Medical Joint Research Project of Chongqing Science and Technology Committee&Health Agency(2020GDRC017)Chongqing Graduate Tutor Team Project(dstd201903)the Medical Research Project of Chongqing Health and Family Planning Commission(2017ZDXM016)。
文摘Local drug delivery has received increasing attention in recent years.However,the therapeutic efficacy of local delivery of drugs is still limited under certain scenarios,such as in the oral cavity or in wound beds after resection of tumors.In this study,we introduce a bioinspired adhesive hydrogel derived from the skin secretions of Andrias davidianus(SSAD)as a wound dressing for localized drug elution.The hydrogel was loaded with aminoguanidine or doxorubicin,and its controlled drug release and healing-promoting properties were verified in a diabetic rat palatal mucosal defect model and a C57BL/6 mouse melanoma-bearing model,respectively.The results showed that SSAD hydrogels with different pore sizes could release drugs in a controllable manner and accelerate wound healing.Transcriptome analyses of the palatal mucosa suggested that SSAD could significantly upregulate pathways linked to cell adhesion and extracellular matrix deposition and had the ability to recruit keratinocyte stem cells to defect sites.Taken together,these findings indicate that property-controllable SSAD hydrogels could be a promising biofunctional wound dressing for local drug delivery and promotion of wound healing.
文摘Background Surgical site infection(SSI)is a common complication following craniotomy that increases morbidity,mortality,and medical expenses.The objectives of this study were to determine the relevant risk factors associated with SSI after elective craniotomy for brain tumor and analyse the treatments for SSI.Methods A retrospective nested case-control study was conducted using data from patients who underwent craniotomy for brain tumor resection at the Neurosurgical Oncology Department No.6 of Beijing Tiantan Hospital,Capital Medical University,between January 2019 and December 2021.Risk factors for SSI were determined using multivariate logistic regression analysis.We analyzed microbiological and related treatment data for different SSI types.Results Among 2061 patients who underwent craniotomy for brain tumor,31 had SSI(1.50%).In the multivariate logistic regression analysis,body mass index(BMI)and operative duration were identified as independent risk factors for SSI.The most common microorganism isolated from SSIs was Staphylococcus epidermidis(22.9%),and drug sensitivity results showed that gram-positive bacteria were sensitive to linezolid,vancomycin and tigecycline,whereas gram-negative bacteria were sensitive to meropenem,cefepime and ceftazidime.Six of the seven patients who underwent bone flap removal due to osteomyelitis were infected with gram-negative bacteria.Conclusions BMI and operative duration were identified as independent risk factors for SSI.Diabetes mellitus,previous ratio therapy,type of incision,recurrence tumor and other risk factors were not found to be associated with the occurrence of SSI in this study.
文摘Direct mixture of Au^3＋ with glutathione （GSH）, which act as both reduction agents and stabilizers, in aqueous solution gave rise to production of gold nanoparticles （Au NPs） with uniform sizes of around 21 nm. The GSH stabilizer Au NPs in solution show immediate aggregation after addition of 1 mol/L NaCI aqueous solution containing Pb^2＋ ions. The Pb^2＋-induced aggregation in Au NP solution is monitored by both colorimetric response and UV-vis spectroscopy. A rather broad linear range （from 0.1 to 30 pmol/L） and low detection limit （0.1 pmol/L） are explored for Au NP sensors used for detection of Pb^2＋ ions. Furthermore, the response of GSH-stabilized Au NPs toward Pb^2＋ ions is specific compared with other possible interferants （Hg^2＋, Mg^2＋, Zn^2＋, Ni^2＋, Cu^2＋, Co^2＋, Ca^2＋, Mn^2＋, Cd^2＋, and Ba^2＋）.
基金support provided to the first author by the Natural Science Foundation of China(No.52201371)the Postdoctoral Science Foundation of China(No.2021M692043)the Postdoctoral Excellence Program of Shanghai(No.2021200)is gratefully acknowledged.
文摘Zero Poisson’s ratio(ZPR)mechanical metamaterials can yield no transverse displacements when unidi-rectionally compressed,and cylindrical sandwich meta-structures composed of semi re-entrant(SRE)ZPR metamaterials are thus explored for applications on cylindrical shells of underwater equipment or sub-mersible structures.A group of ZPR unit cells with specified pre-strained wave propagation characteristics and adequate load-bearing capabilities is optimally designed based on the periodic boundary condition(PBC)and Bloch’s Theorem.The sound transmission and pressure-resistant performance of cylindrical sandwich meta-structures comprising the homogeneous and graded SRE ZPR unit cells are then investi-gated.The results show that the designed meta-structures can perfectly yield better vibroacoustic atten-uation behavior within the specified frequency regions corresponding to the pre-strained band gaps and safely bear the hydrostatic pressure equivalent to 1000 m depth with low weight-bulk ratios.In addition,the functionally graded metamaterial core can boost vibroacoustic performance within broader frequency ranges.
