Objective:To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum.Methods:The cytotoxicity of ligustroside was tested via MTT assay.Furthermore,the effects of ligustroside on th...Objective:To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum.Methods:The cytotoxicity of ligustroside was tested via MTT assay.Furthermore,the effects of ligustroside on the expression of critical melanogenic markers such as tyrosinase,tyrosinase related proteins(TRPs),and microphthalmia-associated transcription factor(MITF)were analyzed at both mRNA and protein levels via RT-qPCR and Western blot,respectively,inα-melanocyte stimulating hormoneinduced B16F10 cells.In addition,phosphorylation of p38,ERK and JNK proteins was investigated.Immunofluorescence analysis of MITF was also conducted.Results:Ligustroside significantly reduced intracellular tyrosinase activity and melanin content by 37.11%and 29.12%,respectively,compared to untreated cells.Moreover,it downregulated the expression of MITF,tyrosinase,TRP-1,and TRP-2 at the mRNA and protein levels by regulating both the mitogen-activated protein kinase(MAPK)and protein kinase A(PKA)/cAMP response element-binding protein(CREB)signaling pathways.Ligustroside also suppressed the nuclear protein expression of MITF,β-catenin,and p-CREB,and decreased immunofluorescence intensity of nuclear MITF.Conclusions:Ligustroside derived from Ligustrum japonicum shows a significant anti-melanogenesis effect via suppression of the MAPK and PKA/CREB signaling pathways.展开更多
Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead...Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead to diagnostic and therapeutic pitfalls. We here report a case of microcystic/reticular schwannoma arising in the meso-appendix of a 43-year-old woman. The tumor was incidentally discovered by computed tomography scan for unrelated reasons. A laparoscopic operation was performed shortly after admission. Histological examination revealed a circumscribed tumor with a striking microcystic and cribriform architecture. Immunohistochemically, the tumor cells were diffusely positive for S100 protein, glial fibrillary acid protein and protein gene product 9.5, which were consistent with a peripheral nerve sheath tumor. The patient remains well with no signs of recurrence at a 10-mo follow-up. To our knowledge, this is the first case of microcystic/reticular schwannoma arising in the meso-appendix. Albeit very rare, microcystic/reticular schwannoma should be included in the differential diagnosis of appendiceal tumors.展开更多
Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of...Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of melanogenesis-related genes and proteins were analyzed inα-melanocyte-stimulating hormone(α-MSH)-stimulated B16F10 cells.Results:α-MSH treatment significantly increased tyrosinase activity,and extracellular and intracellular melanin content,as well as the expression levels of tyrosinase,microphthalmia-associated transcription factor(MITF),tyrosinase-related protein(TRP)-1,and TRP-2 in B16F10 cells.Treatment with Cyrtomium falcatum crude extract and its solvent fractions reduced tyrosinase activity and extracellular and intracellular melanin content and downregulated the expression levels of tyrosinase,MITF,TRP-1,and TRP-2 in a dose-dependent manner.Conclusions:Cyrtomium falcatum has potential anti-melanogenesis effects and can be used as a potential source material in cosmeceutical industry for the research and development of novel lead molecules with whitening properties.展开更多
Based on the beyond-mean-field Skyrme-Hartree-Fock model,impurity effects of theΛhyperon in the hypernuclear systems^(25)ΛMg and^(29)ΛSi are investigated,respectively.Four cases,in which theΛhyperon occupies the s...Based on the beyond-mean-field Skyrme-Hartree-Fock model,impurity effects of theΛhyperon in the hypernuclear systems^(25)ΛMg and^(29)ΛSi are investigated,respectively.Four cases,in which theΛhyperon occupies the single-particle orbitals L[000]1+/2,L[110]1-/2,L[101]3-/2 and L[101]1-/2,are focused.In each case,the potential energy surface and the energy curves projected on certain angular momenta are employed to show the influence of theΛhyperon on the nuclear core.Beside the shrinkage effect that is induced by theΛhyperon occupying the sΛorbital,it is found that theΛhyperon on the pΛorbital,L[110]1-/2,drives the nuclear core toward a prolate shape,while the ones on the other two pΛorbitals,L[101]3-/2 and L[101]1-/2,drive the nuclear core toward an oblate shape.The energy spectra and the corresponding intra-band E2 transition rates for the rotational bands are given as a prediction for future experiments.展开更多
By combining the Skyrme-Hartree-Fock method with complex momentum representation(CMR),the resonant states of_(Λ)^(17)O,_(Λ)^(41)Ca,_(Λ)^(49)Ca,and_(Λ)^(57)Ni were investigated.The phase shifts for hyperon-nucleus ...By combining the Skyrme-Hartree-Fock method with complex momentum representation(CMR),the resonant states of_(Λ)^(17)O,_(Λ)^(41)Ca,_(Λ)^(49)Ca,and_(Λ)^(57)Ni were investigated.The phase shifts for hyperon-nucleus elastic scattering were determined with continuum level density(CLD),and the scattering length as well as the resonance energy were obtained by utilizing the effective range expansion.Our method,abbreviated as CMR-CLD,exhibits good consistency with traditional approaches and provides ground work for investigating scattering and resonance problems in deformed and multi-hyperon hypernuclei.展开更多
The deformations of multi-Λ hypernuclei corresponding to even-even core nuclei ranging from^(8)Be to^(40)Ca with 2,4,6,and 8 hyperons are studied using the deformed Skyrme-Hartree-Fock approach.It is found that the d...The deformations of multi-Λ hypernuclei corresponding to even-even core nuclei ranging from^(8)Be to^(40)Ca with 2,4,6,and 8 hyperons are studied using the deformed Skyrme-Hartree-Fock approach.It is found that the deformations are reduced when adding 2 or 8 Λ hyperons,but enhanced when adding 4 or 6 Λ hyperons.These differences are attributed to the fact that Λ hyperons are filled gradually into the three deformed p orbits,of which the [110] 1/2^(-)orbit is prolately deformed and the degenerate [101]1/2^(-)and [101]3/2^(-)orbits are oblately deformed.展开更多
In this study,we investigate the robustness of pair structures for nuclear yrast states,that is,whether the structures of relevant collective pairs as building blocks of different yrast states are the same.We focus on...In this study,we investigate the robustness of pair structures for nuclear yrast states,that is,whether the structures of relevant collective pairs as building blocks of different yrast states are the same.We focus on deformed and transitional nuclei and study the yrast states of^(28)Si,^(50)Cr,and^(132)Xe,whose experimental R_(4/2)values are 2.60,2.40,and 2.16,respectively,using the nucleon-pair approximation(NPA)and shell-model effective interactions.For each yrast state,we consider optimized pair structures to be those providing the energy minimum for this state.To find the minimum,many full NPA calculations are performed with varying pair structures,and the numerical optimization procedure of the conjugate gradient method is implemented.Our results suggest that optimized pair structures remain the same for all states within a rotational band of a deformed nucleus.Our results also suggest that after backbending,that is,changing of the intrinsic state,the structure of the S pair,which is essential to build the monopole pairing correlation,remains approximately unchanged,whereas the structures of the non-S pairs,which are essential to build the quadrupole correlation,change significantly.展开更多
We present the hypernuclear states of^(37)_ΛAr obtained using the Skyrme-Hartree-Fock(SHF)model and a beyond-mean-field approach,including angular momentum projection(AMP)and the generator coordinate method(GCM).A co...We present the hypernuclear states of^(37)_ΛAr obtained using the Skyrme-Hartree-Fock(SHF)model and a beyond-mean-field approach,including angular momentum projection(AMP)and the generator coordinate method(GCM).A comprehensive energy spectrum is given,which includes normally deformed(ND)and super deformed(SD)hypernuclear states with positive or negative parities.Energy levels corresponding to the configurations in which aΛhyperon occupies the s-,p-,or sd-shell orbitals are discussed.For the s-shellΛ,we pay special attention to the ND and SD states corresponding to the configurations^(36)Ar^(N)\otimes s_Λand^(36)Ar^(S)otimes s_Λ,where^(36)Ar^(N)and^(36)Ar^(S)denote the ND and SD nuclear cores,respectively.The disagreements between different models over theΛseparation energy of the SD state in previous studies are revisited.For the p-shellΛ,four rotational bands are predicted,and the impurity effects are shown.Furthermore,two energy levels corresponding to the configurations^(36)Ar^otimesΛ[101](3)(2)^(-)and^(36)Ar^(S)otimesΛ[101](1)(2)^(-)are obtained below the separation threshold of^(36)Ar+Λwithin 0.5 MeV.For the sd-shellΛ,three bound states are found near the separation threshold,and the mechanism behind these states are discussed.展开更多
The properties of the clusterized structure in ^(21)_(Λ)Ne are stud-ied in detail based on a beyond relativistic mean-field(RMF)model in a recent paper[1],“The interplay of single-particle and collective motions in ...The properties of the clusterized structure in ^(21)_(Λ)Ne are stud-ied in detail based on a beyond relativistic mean-field(RMF)model in a recent paper[1],“The interplay of single-particle and collective motions in the low-lying states of ^(21)_(Λ)Ne with quadrupole-octupole correlations”.The intrinsic mean-field states of ^(21)_(Λ)Ne with quadrupole and octupole constraints are projected on certain angular momenta and parities,and the generator coordinate method is used to simultaneously consider configurational mixing.展开更多
The effects of an additional K-meson on the ground-state properties of nuclei are investigated within an axially-deformed Skyrme-Hartree-Fock approach combined with a Skyrme-type kaon-nucleon interaction.The K-meson i...The effects of an additional K-meson on the ground-state properties of nuclei are investigated within an axially-deformed Skyrme-Hartree-Fock approach combined with a Skyrme-type kaon-nucleon interaction.The K-meson increases the binding energies of all nuclei,whereas it affects deformations only for light nuclei without shell closure.The nucleon drip lines are modified due to the strongly attractive K-N interaction.This is attributed to the behavior of the highest-occupied nucleon single-particle levels near the drip lines,which is analyzed in detail.展开更多
基金supported by the BB21plus funded by Busan Metropolitan City and Busan Techno Park,and the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2023R1A2C1006268 and RS-2023-00212560).
文摘Objective:To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum.Methods:The cytotoxicity of ligustroside was tested via MTT assay.Furthermore,the effects of ligustroside on the expression of critical melanogenic markers such as tyrosinase,tyrosinase related proteins(TRPs),and microphthalmia-associated transcription factor(MITF)were analyzed at both mRNA and protein levels via RT-qPCR and Western blot,respectively,inα-melanocyte stimulating hormoneinduced B16F10 cells.In addition,phosphorylation of p38,ERK and JNK proteins was investigated.Immunofluorescence analysis of MITF was also conducted.Results:Ligustroside significantly reduced intracellular tyrosinase activity and melanin content by 37.11%and 29.12%,respectively,compared to untreated cells.Moreover,it downregulated the expression of MITF,tyrosinase,TRP-1,and TRP-2 at the mRNA and protein levels by regulating both the mitogen-activated protein kinase(MAPK)and protein kinase A(PKA)/cAMP response element-binding protein(CREB)signaling pathways.Ligustroside also suppressed the nuclear protein expression of MITF,β-catenin,and p-CREB,and decreased immunofluorescence intensity of nuclear MITF.Conclusions:Ligustroside derived from Ligustrum japonicum shows a significant anti-melanogenesis effect via suppression of the MAPK and PKA/CREB signaling pathways.
基金Supported by National Key Clinical Specialty Project and Key Clinical Program of the Chinese Ministry of Health and the National Natural Science Foundation of China,No.81272630
文摘Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead to diagnostic and therapeutic pitfalls. We here report a case of microcystic/reticular schwannoma arising in the meso-appendix of a 43-year-old woman. The tumor was incidentally discovered by computed tomography scan for unrelated reasons. A laparoscopic operation was performed shortly after admission. Histological examination revealed a circumscribed tumor with a striking microcystic and cribriform architecture. Immunohistochemically, the tumor cells were diffusely positive for S100 protein, glial fibrillary acid protein and protein gene product 9.5, which were consistent with a peripheral nerve sheath tumor. The patient remains well with no signs of recurrence at a 10-mo follow-up. To our knowledge, this is the first case of microcystic/reticular schwannoma arising in the meso-appendix. Albeit very rare, microcystic/reticular schwannoma should be included in the differential diagnosis of appendiceal tumors.
基金This work was supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2023R1A2C1006268 and RS-2023-00212560).
文摘Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of melanogenesis-related genes and proteins were analyzed inα-melanocyte-stimulating hormone(α-MSH)-stimulated B16F10 cells.Results:α-MSH treatment significantly increased tyrosinase activity,and extracellular and intracellular melanin content,as well as the expression levels of tyrosinase,microphthalmia-associated transcription factor(MITF),tyrosinase-related protein(TRP)-1,and TRP-2 in B16F10 cells.Treatment with Cyrtomium falcatum crude extract and its solvent fractions reduced tyrosinase activity and extracellular and intracellular melanin content and downregulated the expression levels of tyrosinase,MITF,TRP-1,and TRP-2 in a dose-dependent manner.Conclusions:Cyrtomium falcatum has potential anti-melanogenesis effects and can be used as a potential source material in cosmeceutical industry for the research and development of novel lead molecules with whitening properties.
基金supported by the National Natural Science Foundation of China(Nos.11905165,11775081 and 11547044)the Fundamental Research Funds for the Central Universities(Nos.JB160510 and XJS18020).
文摘Based on the beyond-mean-field Skyrme-Hartree-Fock model,impurity effects of theΛhyperon in the hypernuclear systems^(25)ΛMg and^(29)ΛSi are investigated,respectively.Four cases,in which theΛhyperon occupies the single-particle orbitals L[000]1+/2,L[110]1-/2,L[101]3-/2 and L[101]1-/2,are focused.In each case,the potential energy surface and the energy curves projected on certain angular momenta are employed to show the influence of theΛhyperon on the nuclear core.Beside the shrinkage effect that is induced by theΛhyperon occupying the sΛorbital,it is found that theΛhyperon on the pΛorbital,L[110]1-/2,drives the nuclear core toward a prolate shape,while the ones on the other two pΛorbitals,L[101]3-/2 and L[101]1-/2,drive the nuclear core toward an oblate shape.The energy spectra and the corresponding intra-band E2 transition rates for the rotational bands are given as a prediction for future experiments.
基金Supported by the National Natural Science Foundation of China(12035011,11905103,11947211,11761161001,11961141003,12022517,12375122)the National Key R&D Program of China(2023YFA1606503)+1 种基金the Science and Technology Development Fund of Macao,China(0048/2020/A1,008/2017/AFJ)the Fundamental Research Funds for the Central Universities(22120210138,22120200101)。
文摘By combining the Skyrme-Hartree-Fock method with complex momentum representation(CMR),the resonant states of_(Λ)^(17)O,_(Λ)^(41)Ca,_(Λ)^(49)Ca,and_(Λ)^(57)Ni were investigated.The phase shifts for hyperon-nucleus elastic scattering were determined with continuum level density(CLD),and the scattering length as well as the resonance energy were obtained by utilizing the effective range expansion.Our method,abbreviated as CMR-CLD,exhibits good consistency with traditional approaches and provides ground work for investigating scattering and resonance problems in deformed and multi-hyperon hypernuclei.
基金Supported by the National Natural Science Foundation of China (12175071, 11775081, 11905165)。
文摘The deformations of multi-Λ hypernuclei corresponding to even-even core nuclei ranging from^(8)Be to^(40)Ca with 2,4,6,and 8 hyperons are studied using the deformed Skyrme-Hartree-Fock approach.It is found that the deformations are reduced when adding 2 or 8 Λ hyperons,but enhanced when adding 4 or 6 Λ hyperons.These differences are attributed to the fact that Λ hyperons are filled gradually into the three deformed p orbits,of which the [110] 1/2^(-)orbit is prolately deformed and the degenerate [101]1/2^(-)and [101]3/2^(-)orbits are oblately deformed.
基金Supported by the National Natural Science Foundation of China(11875134,11875188,12175071,11975151,11961141003)the Shanghai Key Laboratory of Particle Physics and Cosmology(21DZ2271500-2)。
文摘In this study,we investigate the robustness of pair structures for nuclear yrast states,that is,whether the structures of relevant collective pairs as building blocks of different yrast states are the same.We focus on deformed and transitional nuclei and study the yrast states of^(28)Si,^(50)Cr,and^(132)Xe,whose experimental R_(4/2)values are 2.60,2.40,and 2.16,respectively,using the nucleon-pair approximation(NPA)and shell-model effective interactions.For each yrast state,we consider optimized pair structures to be those providing the energy minimum for this state.To find the minimum,many full NPA calculations are performed with varying pair structures,and the numerical optimization procedure of the conjugate gradient method is implemented.Our results suggest that optimized pair structures remain the same for all states within a rotational band of a deformed nucleus.Our results also suggest that after backbending,that is,changing of the intrinsic state,the structure of the S pair,which is essential to build the monopole pairing correlation,remains approximately unchanged,whereas the structures of the non-S pairs,which are essential to build the quadrupole correlation,change significantly.
基金Supported by the National Natural Science Foundation of China (11905165, 11775081, 12175071, 11547044)the 111 Project (B17035)the Fundamental Research Funds for the Central Universities (JB160510, XJS18020)
文摘We present the hypernuclear states of^(37)_ΛAr obtained using the Skyrme-Hartree-Fock(SHF)model and a beyond-mean-field approach,including angular momentum projection(AMP)and the generator coordinate method(GCM).A comprehensive energy spectrum is given,which includes normally deformed(ND)and super deformed(SD)hypernuclear states with positive or negative parities.Energy levels corresponding to the configurations in which aΛhyperon occupies the s-,p-,or sd-shell orbitals are discussed.For the s-shellΛ,we pay special attention to the ND and SD states corresponding to the configurations^(36)Ar^(N)\otimes s_Λand^(36)Ar^(S)otimes s_Λ,where^(36)Ar^(N)and^(36)Ar^(S)denote the ND and SD nuclear cores,respectively.The disagreements between different models over theΛseparation energy of the SD state in previous studies are revisited.For the p-shellΛ,four rotational bands are predicted,and the impurity effects are shown.Furthermore,two energy levels corresponding to the configurations^(36)Ar^otimesΛ[101](3)(2)^(-)and^(36)Ar^(S)otimesΛ[101](1)(2)^(-)are obtained below the separation threshold of^(36)Ar+Λwithin 0.5 MeV.For the sd-shellΛ,three bound states are found near the separation threshold,and the mechanism behind these states are discussed.
文摘The properties of the clusterized structure in ^(21)_(Λ)Ne are stud-ied in detail based on a beyond relativistic mean-field(RMF)model in a recent paper[1],“The interplay of single-particle and collective motions in the low-lying states of ^(21)_(Λ)Ne with quadrupole-octupole correlations”.The intrinsic mean-field states of ^(21)_(Λ)Ne with quadrupole and octupole constraints are projected on certain angular momenta and parities,and the generator coordinate method is used to simultaneously consider configurational mixing.
基金Supported by the National Natural Science Foundation of China(12175071,11775081)。
文摘The effects of an additional K-meson on the ground-state properties of nuclei are investigated within an axially-deformed Skyrme-Hartree-Fock approach combined with a Skyrme-type kaon-nucleon interaction.The K-meson increases the binding energies of all nuclei,whereas it affects deformations only for light nuclei without shell closure.The nucleon drip lines are modified due to the strongly attractive K-N interaction.This is attributed to the behavior of the highest-occupied nucleon single-particle levels near the drip lines,which is analyzed in detail.
