摘要
Objective:To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum.Methods:The cytotoxicity of ligustroside was tested via MTT assay.Furthermore,the effects of ligustroside on the expression of critical melanogenic markers such as tyrosinase,tyrosinase related proteins(TRPs),and microphthalmia-associated transcription factor(MITF)were analyzed at both mRNA and protein levels via RT-qPCR and Western blot,respectively,inα-melanocyte stimulating hormoneinduced B16F10 cells.In addition,phosphorylation of p38,ERK and JNK proteins was investigated.Immunofluorescence analysis of MITF was also conducted.Results:Ligustroside significantly reduced intracellular tyrosinase activity and melanin content by 37.11%and 29.12%,respectively,compared to untreated cells.Moreover,it downregulated the expression of MITF,tyrosinase,TRP-1,and TRP-2 at the mRNA and protein levels by regulating both the mitogen-activated protein kinase(MAPK)and protein kinase A(PKA)/cAMP response element-binding protein(CREB)signaling pathways.Ligustroside also suppressed the nuclear protein expression of MITF,β-catenin,and p-CREB,and decreased immunofluorescence intensity of nuclear MITF.Conclusions:Ligustroside derived from Ligustrum japonicum shows a significant anti-melanogenesis effect via suppression of the MAPK and PKA/CREB signaling pathways.
基金
supported by the BB21plus funded by Busan Metropolitan City and Busan Techno Park,and the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2023R1A2C1006268 and RS-2023-00212560).
