以[^(14)C]碳酸钡为放射性同位素原料,通过乙炔环三聚、苯羧基化和甲基化、胺化、环化、亲核取代、格氏、还原、氧化等反应和反相高效液相色谱(HPLC)纯化获得了2种放射性同位素碳-14标记毒氟磷[N-[2-(4-甲基[苯基-U-^(14)C_6]苯并噻唑基...以[^(14)C]碳酸钡为放射性同位素原料,通过乙炔环三聚、苯羧基化和甲基化、胺化、环化、亲核取代、格氏、还原、氧化等反应和反相高效液相色谱(HPLC)纯化获得了2种放射性同位素碳-14标记毒氟磷[N-[2-(4-甲基[苯基-U-^(14)C_6]苯并噻唑基)]-2-氨基-2-氟苯基-O,O-二乙基甲基膦酸酯(2,20.6 m Ci)和N-[2-(4-甲基苯并噻唑基)]-2-氨基-2-氟苯基-O,O-二乙基[^(14)C]甲基膦酸酯(3,32.4 m Ci)],其结构经核磁共振氢谱(~1H NMR)和在线放射性高效液相色谱-二极管阵列检测器/质谱联用(HPLC-FSA/PDA/MS)分析确认,反应总放化收率/化学收率分别为31%和67%.放射性薄层层析-同位素成像分析(TLC-IIA)、离线放射性高效液相色谱(HPLC-LSC),HPLC-FSA/PDA/MS和液体闪烁测量(LSC)分析表明,两种标记物的放化纯度和化学纯度均大于98%,比活度分别为25.5,55.5 m Ci/mmol.该标记物可作为放射性示踪剂,用于毒氟磷的代谢、残留和环境行为等研究.展开更多
Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particul...Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.展开更多
文摘以[^(14)C]碳酸钡为放射性同位素原料,通过乙炔环三聚、苯羧基化和甲基化、胺化、环化、亲核取代、格氏、还原、氧化等反应和反相高效液相色谱(HPLC)纯化获得了2种放射性同位素碳-14标记毒氟磷[N-[2-(4-甲基[苯基-U-^(14)C_6]苯并噻唑基)]-2-氨基-2-氟苯基-O,O-二乙基甲基膦酸酯(2,20.6 m Ci)和N-[2-(4-甲基苯并噻唑基)]-2-氨基-2-氟苯基-O,O-二乙基[^(14)C]甲基膦酸酯(3,32.4 m Ci)],其结构经核磁共振氢谱(~1H NMR)和在线放射性高效液相色谱-二极管阵列检测器/质谱联用(HPLC-FSA/PDA/MS)分析确认,反应总放化收率/化学收率分别为31%和67%.放射性薄层层析-同位素成像分析(TLC-IIA)、离线放射性高效液相色谱(HPLC-LSC),HPLC-FSA/PDA/MS和液体闪烁测量(LSC)分析表明,两种标记物的放化纯度和化学纯度均大于98%,比活度分别为25.5,55.5 m Ci/mmol.该标记物可作为放射性示踪剂,用于毒氟磷的代谢、残留和环境行为等研究.
基金supported by the National Key R&D Program(No.2018YFD0900301-03)the MNR Key Laboratory of Marine Eco-Environmental Science and Technology,China(No.MEEST-2021-04).
文摘Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.
