Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly importan...Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly important in regulating animal behavior,but the function of serotonin in plants is far less known.According to recent research,serotonin is vital for plant growth,development,and stress responses,achieved through transcriptional and phytohormonal interplay.Specifically,this review addresses critical gaps in the understanding of serotonin's function in plants by examining its biosynthesis,metabolism,and its multifaceted role in mitigating both abiotic stresses(salinity,drought,heat,cold,and heavy metals)as well as biotic challenges(pathogens,pests,and herbivores).As a pivotal player,it engages in a variety of significant cellular and molecular interactions,including those with reactive oxygen and nitrogen species(RONS),and various phytohormones such as auxin,abscisic acid(ABA),salicylic acid(SA),jasmonic acid(JA),ethylene(ET),and cytokinin(CK).Advances in serotonin-related research are anticipated to offer a valuable basis for uncovering the regulatory pathways by which serotonin impacts the resilience of crops against abiotic stress.展开更多
Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monocul...Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monoculm1(MOC1),a critical TF controlling tiller number and plant height in rice,positively regulates rice cold tolerance at the seedling stage.We found that OsMPK4,a mitogen-activated protein kinase,phosphorylates and stabilizes MOC1 under cold stress.Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress.Notably,we found that the OsbZIP79-MOC1-OsNAC5 complex activates several cold-responsive genes,including Dehydration-responsive element-binding factor 1G(OsDREB1G),to confer rice cold tolerance.Haplotype analysis of the OsDREB1G promoter in>10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance.Collectively,our work demonstrates a pivotal role of the OsMPK4-OsbZIP79-MOC1-OsNAC5-OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.展开更多
基金supported by the National Science Foundation of China(32460077)the“Nanhai New Star”Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362)+1 种基金the Scientific Research Fund of Zhejiang Provincial Education Department(Y202353681)the Initial Scientific Research Fund of Hainan Institute,Zhejiang University(0201-6602-A12203).
文摘Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly important in regulating animal behavior,but the function of serotonin in plants is far less known.According to recent research,serotonin is vital for plant growth,development,and stress responses,achieved through transcriptional and phytohormonal interplay.Specifically,this review addresses critical gaps in the understanding of serotonin's function in plants by examining its biosynthesis,metabolism,and its multifaceted role in mitigating both abiotic stresses(salinity,drought,heat,cold,and heavy metals)as well as biotic challenges(pathogens,pests,and herbivores).As a pivotal player,it engages in a variety of significant cellular and molecular interactions,including those with reactive oxygen and nitrogen species(RONS),and various phytohormones such as auxin,abscisic acid(ABA),salicylic acid(SA),jasmonic acid(JA),ethylene(ET),and cytokinin(CK).Advances in serotonin-related research are anticipated to offer a valuable basis for uncovering the regulatory pathways by which serotonin impacts the resilience of crops against abiotic stress.
基金supported by the National Natural Science Foundation of China(32570362,32460077,and 32570749)the Hainan Province Science and Technology Special Fund(ZDYF2025XDNY093)+4 种基金the Hainan Provincial Natural Science Foundation of China(325RC799)the Nanhai New Star Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362)the Research Startup Funding from Hainan Institute of Zhejiang University(0201-6602-A12203)the Open Project Program of the State Key Laboratory of Rice Biology and Breeding(20240101)the Open Project Program of Jiaxing Academy of Agricultural Sciences(202405).
文摘Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monoculm1(MOC1),a critical TF controlling tiller number and plant height in rice,positively regulates rice cold tolerance at the seedling stage.We found that OsMPK4,a mitogen-activated protein kinase,phosphorylates and stabilizes MOC1 under cold stress.Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress.Notably,we found that the OsbZIP79-MOC1-OsNAC5 complex activates several cold-responsive genes,including Dehydration-responsive element-binding factor 1G(OsDREB1G),to confer rice cold tolerance.Haplotype analysis of the OsDREB1G promoter in>10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance.Collectively,our work demonstrates a pivotal role of the OsMPK4-OsbZIP79-MOC1-OsNAC5-OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.
