摘要
Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monoculm1(MOC1),a critical TF controlling tiller number and plant height in rice,positively regulates rice cold tolerance at the seedling stage.We found that OsMPK4,a mitogen-activated protein kinase,phosphorylates and stabilizes MOC1 under cold stress.Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress.Notably,we found that the OsbZIP79-MOC1-OsNAC5 complex activates several cold-responsive genes,including Dehydration-responsive element-binding factor 1G(OsDREB1G),to confer rice cold tolerance.Haplotype analysis of the OsDREB1G promoter in>10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance.Collectively,our work demonstrates a pivotal role of the OsMPK4-OsbZIP79-MOC1-OsNAC5-OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.
基金
supported by the National Natural Science Foundation of China(32570362,32460077,and 32570749)
the Hainan Province Science and Technology Special Fund(ZDYF2025XDNY093)
the Hainan Provincial Natural Science Foundation of China(325RC799)
the Nanhai New Star Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362)
the Research Startup Funding from Hainan Institute of Zhejiang University(0201-6602-A12203)
the Open Project Program of the State Key Laboratory of Rice Biology and Breeding(20240101)
the Open Project Program of Jiaxing Academy of Agricultural Sciences(202405).
