Based on schematically formulation of the vibrations induced by moving trains, this paper analyses the waveforms along the Datong-Qinhuangdao railroad in Northern China recorded in the suburban Huairou district of Be...Based on schematically formulation of the vibrations induced by moving trains, this paper analyses the waveforms along the Datong-Qinhuangdao railroad in Northern China recorded in the suburban Huairou district of Beijing on March 8, 2003. It is illustrated that vibrations induced by train, except traditional recognized noises and interfer- ence effects, could be used as a seismic source to detect crustal structures with its advantage of abundant frequency spectrum, repeatability and no additional harm to the environment. It will bring lights to the traditional exploration seismology with the further studies of signal processing and interpretation methods, and related models and new observing systems.展开更多
Objective： This study is aimed at developing a simple and easy way to generate dendritic cells （DCs） from human peripheral blood monocytes （PBMCs） in vitro. Methods： PBMCs were isolated directly from white blood...Objective： This study is aimed at developing a simple and easy way to generate dendritic cells （DCs） from human peripheral blood monocytes （PBMCs） in vitro. Methods： PBMCs were isolated directly from white blood cell rather than whole blood and purified by patching methods （collecting the attached cell and removing the suspension cell）. DCs were then generated by culturing PBMCs for six days with 30 ng/ml recombinant human granulocyte-macrophage stimulating factor （rhGM-CSF） and 20 ng/ml recombinant human interleukin-4 （rhIL-4） in vitro. On the sixth day, TNF-alpha （TNFα） 30 ng/ml was added into some DC cultures, which were then incubated for two additional days. The morphology was monitored by light microscopy and transmission electronic microscopy, and the phenotypes were determined by flow cytometry. Autologous mixed leukocyte reactions （MLR） were used to characterize DC function after TNFα or lipopolysaccharide （LPS） stimulations for 24 h. Results： After six days of culture, the monocytes developed significant dendritic morphology and a portion of cells expressed CDIa, CD80 and CD86, features of DCs. TNFα treatment induced DCs maturation and up-regulation of CD80, CD86 and CD83. Autologous MLR demonstrated that these DCs possess potent T-cell stimulatory capacity. Conclusion： This study developed a simple and easy way to generate DCs from PBMCs exposed to rhGM-CSF and rhIL-4. The DCs produced by this method acquired morphologic and antigenic characteristics of DCs.展开更多
Given the multiple terrorist attacks that have occurred in recent years in China, medical rescue teams and specialized incident assessment teams have been established by the government; however, medical rescue after n...Given the multiple terrorist attacks that have occurred in recent years in China, medical rescue teams and specialized incident assessment teams have been established by the government; however, medical rescue after nuclear, biological, and chemical incidents remains challenging and is often inefficient. In the present article, problems were analyzed regarding the assessment of responder countermeasures, training of professionals and the management of emergency medical incidents related to nuclear, biological and chemical attacks. Countermeasures, the establishment of response coordination, public education, practical training and exercise, and a professional consultant team or system should be the focus of emergency medical response facilities. Moreover, the government was offered professionals who are involved in managing nuclear, biological and chemical incidents.展开更多
The nonlinear evolution problem in nonparallel boundary layer stability was studied. The relative parabolized stability equations of nonlinear nonparallel boundary layer were derived. The developed numerical method, w...The nonlinear evolution problem in nonparallel boundary layer stability was studied. The relative parabolized stability equations of nonlinear nonparallel boundary layer were derived. The developed numerical method, which is very effective, was used to study the nonlinear evolution. of T-S disturbance wave at finite amplitudes. Solving nonlinear equations of different modes by using predictor-corrector and iterative approach, which is uncoupled between modes, improving computational accuracy by using high order compact differential scheme, satisfying normalization condition I determining tables of nonlinear terms at different modes, and implementing stably the spatial marching, were included in this method. With different initial amplitudes, the nonlinear evolution of T-S wave was studied. The nonlinear nonparallel results of examples compare with data of direct numerical simulations (DNS) using full Navier-Stokes equations.展开更多
Using the basic solutions of a single crack and a single inclusion, and making use of the principle of linear superposition of elastic mechanics, the interaction problem between a planar crack and a flat inclusion in ...Using the basic solutions of a single crack and a single inclusion, and making use of the principle of linear superposition of elastic mechanics, the interaction problem between a planar crack and a flat inclusion in an elastic solid is studied. The problem is reduced to solve a set of standard Cauchy-type singular equations. And the stress intensity factors at points of crack and inclusion were obtained. Besides, the singularity for the horizontal contact of crack and inclusion was analyzed. The calculating model put forward can be regarded as a new technique for studying the crack and its expanding caused by inclusion lip. Then several numerical examples are given.展开更多
Using the single crack solution and the regular solution elf plane harmonic function, the problem of Saint-Venant bending of a cracked cylinder by a transverse force was reduced to solving two sets of integral equatio...Using the single crack solution and the regular solution elf plane harmonic function, the problem of Saint-Venant bending of a cracked cylinder by a transverse force was reduced to solving two sets of integral equations and its general solution was then obtained. Based on the obtained solution, a method to calculate the bending center and the stress intensity factors of the cracked cylinger whose cross-section is not thin-walled, but of small torsion rigidity is proposed. Some numerical examples are given.展开更多
Based on the strain formulation of the quasi-conforming finite element, displacement functions are constructed which have definite physical meaning, and a conclusion can be obtained that the coefficients of the consta...Based on the strain formulation of the quasi-conforming finite element, displacement functions are constructed which have definite physical meaning, and a conclusion can be obtained that the coefficients of the constant and the linear strain are uniquely determined, and the quasi-conforming finite element method is convergent to constant strain. There are different methods for constructing the rigid displacement items, and different methods correspond to different order node errors, and this is different from ordinary displacement method finite element.展开更多
Summary: To investigate the changes in neurological symptoms and signs, as well as serum copper, serum ceruloplasmin after hepatic transplantation in patients with Wilson’s disease, neurological symptoms and signs, s...Summary: To investigate the changes in neurological symptoms and signs, as well as serum copper, serum ceruloplasmin after hepatic transplantation in patients with Wilson’s disease, neurological symptoms and signs, serum copper, serum ceruloplasmin before and after hepatic transplantation in 18 patients with Wilson’s disease were observed, and those changes were followed up in 20 non-operative controls treated with penicillamine. Our results showed that the neurological symptoms and signs, serum copper and serum ceruloplasmin were improved in the operative group but deteriorated in the non-operative control group. Our study showed that hepatic transplantation is better than penicillamine in the treatment of Wilson’s disease.展开更多
The food consumption and ecological conversion efficiency of a species marine pelagic andsmall size fish, Hyporhamphus sajori, were determined by using in situ stomach content method presented by Eggers. The results s...The food consumption and ecological conversion efficiency of a species marine pelagic andsmall size fish, Hyporhamphus sajori, were determined by using in situ stomach content method presented by Eggers. The results showed that: (1) the fish was taken in food all day, so empty-stomach rate was very low, taking up about 4.5% of the total determined fish number. However, the fish still has significant daily feeding rhythm. A feeding peak was found 0:00 o'clock at night, but feeding level was always high in the daytime; (2) relationship between instantaneous food content in stomach and corresponding time could be described as S_t = a · e (-b·t). There was not significant difference of instantaneous gastric evacuation rate between two determinations, with average value being 0.13 × 10^(-2) g/ (g' h) (wet weight); (3) the daily food consumption tended to change in irregular waving form, with average value being (10.16Xl0^(-2)±1.19xl0(-2) g/ (g·d) (wet weight) or (55.56 × 10(-2) ±6.51 × 10^(-2) kJ/ (g·d). The wave distance is constant and about 14 d, but the wave height changed largely; (4) actual determining value of daily growth rate was 3.24 × 10^(-2)g/ (g·d) (wet weight) or 12.91 × 10^(-2)kJ/ (g·d), from which 31.89% (wet weight) or 23.24% kJ of ecological conversion efficiency could be obtained.展开更多
基金National Natural Science Foundations of China (No. 40234038 and 40174014).
文摘Based on schematically formulation of the vibrations induced by moving trains, this paper analyses the waveforms along the Datong-Qinhuangdao railroad in Northern China recorded in the suburban Huairou district of Beijing on March 8, 2003. It is illustrated that vibrations induced by train, except traditional recognized noises and interfer- ence effects, could be used as a seismic source to detect crustal structures with its advantage of abundant frequency spectrum, repeatability and no additional harm to the environment. It will bring lights to the traditional exploration seismology with the further studies of signal processing and interpretation methods, and related models and new observing systems.
文摘Objective： This study is aimed at developing a simple and easy way to generate dendritic cells （DCs） from human peripheral blood monocytes （PBMCs） in vitro. Methods： PBMCs were isolated directly from white blood cell rather than whole blood and purified by patching methods （collecting the attached cell and removing the suspension cell）. DCs were then generated by culturing PBMCs for six days with 30 ng/ml recombinant human granulocyte-macrophage stimulating factor （rhGM-CSF） and 20 ng/ml recombinant human interleukin-4 （rhIL-4） in vitro. On the sixth day, TNF-alpha （TNFα） 30 ng/ml was added into some DC cultures, which were then incubated for two additional days. The morphology was monitored by light microscopy and transmission electronic microscopy, and the phenotypes were determined by flow cytometry. Autologous mixed leukocyte reactions （MLR） were used to characterize DC function after TNFα or lipopolysaccharide （LPS） stimulations for 24 h. Results： After six days of culture, the monocytes developed significant dendritic morphology and a portion of cells expressed CDIa, CD80 and CD86, features of DCs. TNFα treatment induced DCs maturation and up-regulation of CD80, CD86 and CD83. Autologous MLR demonstrated that these DCs possess potent T-cell stimulatory capacity. Conclusion： This study developed a simple and easy way to generate DCs from PBMCs exposed to rhGM-CSF and rhIL-4. The DCs produced by this method acquired morphologic and antigenic characteristics of DCs.
基金supported by a grant from the Practical Research of General Logistics Department, Chinese PLA (No. 13BJYZ52)
文摘Given the multiple terrorist attacks that have occurred in recent years in China, medical rescue teams and specialized incident assessment teams have been established by the government; however, medical rescue after nuclear, biological, and chemical incidents remains challenging and is often inefficient. In the present article, problems were analyzed regarding the assessment of responder countermeasures, training of professionals and the management of emergency medical incidents related to nuclear, biological and chemical attacks. Countermeasures, the establishment of response coordination, public education, practical training and exercise, and a professional consultant team or system should be the focus of emergency medical response facilities. Moreover, the government was offered professionals who are involved in managing nuclear, biological and chemical incidents.
文摘The nonlinear evolution problem in nonparallel boundary layer stability was studied. The relative parabolized stability equations of nonlinear nonparallel boundary layer were derived. The developed numerical method, which is very effective, was used to study the nonlinear evolution. of T-S disturbance wave at finite amplitudes. Solving nonlinear equations of different modes by using predictor-corrector and iterative approach, which is uncoupled between modes, improving computational accuracy by using high order compact differential scheme, satisfying normalization condition I determining tables of nonlinear terms at different modes, and implementing stably the spatial marching, were included in this method. With different initial amplitudes, the nonlinear evolution of T-S wave was studied. The nonlinear nonparallel results of examples compare with data of direct numerical simulations (DNS) using full Navier-Stokes equations.
文摘Using the basic solutions of a single crack and a single inclusion, and making use of the principle of linear superposition of elastic mechanics, the interaction problem between a planar crack and a flat inclusion in an elastic solid is studied. The problem is reduced to solve a set of standard Cauchy-type singular equations. And the stress intensity factors at points of crack and inclusion were obtained. Besides, the singularity for the horizontal contact of crack and inclusion was analyzed. The calculating model put forward can be regarded as a new technique for studying the crack and its expanding caused by inclusion lip. Then several numerical examples are given.
文摘Using the single crack solution and the regular solution elf plane harmonic function, the problem of Saint-Venant bending of a cracked cylinder by a transverse force was reduced to solving two sets of integral equations and its general solution was then obtained. Based on the obtained solution, a method to calculate the bending center and the stress intensity factors of the cracked cylinger whose cross-section is not thin-walled, but of small torsion rigidity is proposed. Some numerical examples are given.
文摘Based on the strain formulation of the quasi-conforming finite element, displacement functions are constructed which have definite physical meaning, and a conclusion can be obtained that the coefficients of the constant and the linear strain are uniquely determined, and the quasi-conforming finite element method is convergent to constant strain. There are different methods for constructing the rigid displacement items, and different methods correspond to different order node errors, and this is different from ordinary displacement method finite element.
文摘Summary: To investigate the changes in neurological symptoms and signs, as well as serum copper, serum ceruloplasmin after hepatic transplantation in patients with Wilson’s disease, neurological symptoms and signs, serum copper, serum ceruloplasmin before and after hepatic transplantation in 18 patients with Wilson’s disease were observed, and those changes were followed up in 20 non-operative controls treated with penicillamine. Our results showed that the neurological symptoms and signs, serum copper and serum ceruloplasmin were improved in the operative group but deteriorated in the non-operative control group. Our study showed that hepatic transplantation is better than penicillamine in the treatment of Wilson’s disease.
基金This study was supported by the DevelopmentProgramming Program of National Major Basic Research of China un- der contract No.G19990437.
文摘The food consumption and ecological conversion efficiency of a species marine pelagic andsmall size fish, Hyporhamphus sajori, were determined by using in situ stomach content method presented by Eggers. The results showed that: (1) the fish was taken in food all day, so empty-stomach rate was very low, taking up about 4.5% of the total determined fish number. However, the fish still has significant daily feeding rhythm. A feeding peak was found 0:00 o'clock at night, but feeding level was always high in the daytime; (2) relationship between instantaneous food content in stomach and corresponding time could be described as S_t = a · e (-b·t). There was not significant difference of instantaneous gastric evacuation rate between two determinations, with average value being 0.13 × 10^(-2) g/ (g' h) (wet weight); (3) the daily food consumption tended to change in irregular waving form, with average value being (10.16Xl0^(-2)±1.19xl0(-2) g/ (g·d) (wet weight) or (55.56 × 10(-2) ±6.51 × 10^(-2) kJ/ (g·d). The wave distance is constant and about 14 d, but the wave height changed largely; (4) actual determining value of daily growth rate was 3.24 × 10^(-2)g/ (g·d) (wet weight) or 12.91 × 10^(-2)kJ/ (g·d), from which 31.89% (wet weight) or 23.24% kJ of ecological conversion efficiency could be obtained.
