Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCU...Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCUS T） gene was involved in the photoperiod pathway to regulate fowering in the model plant. In this study, transcriptome sequencing method was used to obtain cDNA sequence of FT homologous gene in onion, named AcFT3 （KF864665）. AcFT3 had a full-length of 540 bp, encoded 179 amino acids, with 98.31% homology to AfFT （Allium fstulosum）, and 63.0%-84.0% homology to other higher plants. Phylogenetic tree analysis indicated that AcFT3 had the closest relationship with AfFT. The results of quantitative RT-PCR showed the expression pattern of AcFT3 both in vegetative growth of onion and in different organs of bolting and fowering, the expression level of AcFT3 reached the highest in the leaves before bolting and in the fower organs after bolting.展开更多
基金Supported by Fund Project of Heilongjiang Province(C2015017)Heilongjiang Provincial Science and Technology Commissioner Project(GC13B09)
文摘Onion （Allium cepa L.） was an economic vegetable and a strictly biennial herb, which was widely distributed in the world. In the past, it was a strictly biennial plant, the studies had shown that FT （FLOWERING LOCUS T） gene was involved in the photoperiod pathway to regulate fowering in the model plant. In this study, transcriptome sequencing method was used to obtain cDNA sequence of FT homologous gene in onion, named AcFT3 （KF864665）. AcFT3 had a full-length of 540 bp, encoded 179 amino acids, with 98.31% homology to AfFT （Allium fstulosum）, and 63.0%-84.0% homology to other higher plants. Phylogenetic tree analysis indicated that AcFT3 had the closest relationship with AfFT. The results of quantitative RT-PCR showed the expression pattern of AcFT3 both in vegetative growth of onion and in different organs of bolting and fowering, the expression level of AcFT3 reached the highest in the leaves before bolting and in the fower organs after bolting.
