This study investigated hypoxia-inducible factor(HIF)-1α-mediated proteomic changes in post-slaughter Tan sheep skeletal muscle and identified energy metabolism biomarkers using the competitive adaptive reweighted sa...This study investigated hypoxia-inducible factor(HIF)-1α-mediated proteomic changes in post-slaughter Tan sheep skeletal muscle and identified energy metabolism biomarkers using the competitive adaptive reweighted sampling(CARS)algorithm.HIF-1αinhibition during early storage attenuated pH decline and significantly increased total colour change(ΔE)(P<0.05)while reducing myofibril fragmentation compared with controls.Proteomic profiling identified 257 differentially expressed proteins enriched in adenosine 5’-monophosphate(AMP)-activated protein kinase(AMPK),glycolysis,and HIF-1 signalling pathways.CARS analysis highlighted lactate dehydrogenase A(LDHA),phosphoglycerate kinase 1(PGK1;glycolytic enzyme),heat shock protein beta-6(HSPB6),and heat shock protein 90 kDa beta 1(HSP90B1)as key energy metabolism biomarkers.The results suggested that HIF-1 stabilised ATP production under hypoxia conditions by suppressing glycogen synthesis,enhancing glycolysis,modulating HSP activity to preserve cellular homeostasis,and influencing cytoskeletal proteins,thereby affecting meat quality.These results provide novel insights into post-mortem muscle energy metabolism regulation and potential targets for meat quality optimisation.展开更多
This study investigated the dynamic synergy of hypoxia-inducible factor-1(HIF-1)in energy metabolism after the slaughter of Tan sheep and its impact on meat color development.The longest dorsal muscle was incubated wi...This study investigated the dynamic synergy of hypoxia-inducible factor-1(HIF-1)in energy metabolism after the slaughter of Tan sheep and its impact on meat color development.The longest dorsal muscle was incubated with the HIF-1 inhibitor YC-1 or 0.9%saline(the control)at 4℃for 0,1 and 2 days.Results from proteomics and enzyme activity assays showed that the activation of HIF-1 enhanced the activities of glycolytic enzymes,particularly phosphofructokinase and phosphoglycerate kinase,while inhibiting the electron transport chain.Proteomic analysis revealed significant changes in the expression of these proteins,indicating that HIF-1 regu-lates energy supply and demand allocation through signaling pathways,such as AMPK and AK.This led to changes in ATP levels,increased AMP and ADP concentrations,and an elevated energy charge in the inhibition group,highlighting the critical role of HIF-1 in post-mortem muscle energy metabolism and meat color stability.展开更多
基金supported by the Innovation Research Group Project of the National Natural Science Foundation of China(No.32260555).
文摘This study investigated hypoxia-inducible factor(HIF)-1α-mediated proteomic changes in post-slaughter Tan sheep skeletal muscle and identified energy metabolism biomarkers using the competitive adaptive reweighted sampling(CARS)algorithm.HIF-1αinhibition during early storage attenuated pH decline and significantly increased total colour change(ΔE)(P<0.05)while reducing myofibril fragmentation compared with controls.Proteomic profiling identified 257 differentially expressed proteins enriched in adenosine 5’-monophosphate(AMP)-activated protein kinase(AMPK),glycolysis,and HIF-1 signalling pathways.CARS analysis highlighted lactate dehydrogenase A(LDHA),phosphoglycerate kinase 1(PGK1;glycolytic enzyme),heat shock protein beta-6(HSPB6),and heat shock protein 90 kDa beta 1(HSP90B1)as key energy metabolism biomarkers.The results suggested that HIF-1 stabilised ATP production under hypoxia conditions by suppressing glycogen synthesis,enhancing glycolysis,modulating HSP activity to preserve cellular homeostasis,and influencing cytoskeletal proteins,thereby affecting meat quality.These results provide novel insights into post-mortem muscle energy metabolism regulation and potential targets for meat quality optimisation.
基金supported by the Innovation Research Group Project of the National Natural Science Foundation of China[32260555].
文摘This study investigated the dynamic synergy of hypoxia-inducible factor-1(HIF-1)in energy metabolism after the slaughter of Tan sheep and its impact on meat color development.The longest dorsal muscle was incubated with the HIF-1 inhibitor YC-1 or 0.9%saline(the control)at 4℃for 0,1 and 2 days.Results from proteomics and enzyme activity assays showed that the activation of HIF-1 enhanced the activities of glycolytic enzymes,particularly phosphofructokinase and phosphoglycerate kinase,while inhibiting the electron transport chain.Proteomic analysis revealed significant changes in the expression of these proteins,indicating that HIF-1 regu-lates energy supply and demand allocation through signaling pathways,such as AMPK and AK.This led to changes in ATP levels,increased AMP and ADP concentrations,and an elevated energy charge in the inhibition group,highlighting the critical role of HIF-1 in post-mortem muscle energy metabolism and meat color stability.
