摘要
This study investigated the dynamic synergy of hypoxia-inducible factor-1(HIF-1)in energy metabolism after the slaughter of Tan sheep and its impact on meat color development.The longest dorsal muscle was incubated with the HIF-1 inhibitor YC-1 or 0.9%saline(the control)at 4℃for 0,1 and 2 days.Results from proteomics and enzyme activity assays showed that the activation of HIF-1 enhanced the activities of glycolytic enzymes,particularly phosphofructokinase and phosphoglycerate kinase,while inhibiting the electron transport chain.Proteomic analysis revealed significant changes in the expression of these proteins,indicating that HIF-1 regu-lates energy supply and demand allocation through signaling pathways,such as AMPK and AK.This led to changes in ATP levels,increased AMP and ADP concentrations,and an elevated energy charge in the inhibition group,highlighting the critical role of HIF-1 in post-mortem muscle energy metabolism and meat color stability.
基金
supported by the Innovation Research Group Project of the National Natural Science Foundation of China[32260555].
