Stigma exsertion in male sterile lines of hybrid rice is important for seed yield.In the present study, ZS616 [Oryza sativa subsp.Xian(indica)], a male sterile line with a stigma exsertion rate(SER) as high as 94.5%, ...Stigma exsertion in male sterile lines of hybrid rice is important for seed yield.In the present study, ZS616 [Oryza sativa subsp.Xian(indica)], a male sterile line with a stigma exsertion rate(SER) as high as 94.5%, was crossed to DS552, a japonica line with almost no exserted stigmas.F3 plants with extremely low and high SER were sequenced to identify SER-associated quantitative trait loci(QTL).A major QTL for SER, qSER-3.1, was identified along with other QTL on chromosome 3 in a 3.9 Mb region.A total of 307 nonsynonymous single-nucleotide polymorphisms(SNPs) and 27 frame-shift insertion/deletions(InDels)differentiating ZS616 and DS552 were identified in the region containing qSER-3.1.Most SNPs(294) and InDels(25) were excluded after further analysis because they were shared by ZS616 and low(<2.0%) SER accessions in the Huazhong Agricultural University(HAU) core rice collection.Association analysis using the full HAU collection identified a 17-bp InDel in OS03 G0689400 as the most likely causal genetic variant underlying qSER-3.1.ZS616-type accessions(n = 54, with the 17-bp insertion) in the HAU collection had minimum(16.5%)and mean(39.6%) SERs significantly greater than those(n = 424) without the insertion(with minimum and mean SERs of 0.2% and 20.6%, respectively).Thus, this study identified a major QTL for stigma exsertion and revealed the mutation in a candidate gene for the QTL.展开更多
Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease inf...Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host–pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research.展开更多
Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly importan...Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly important in regulating animal behavior,but the function of serotonin in plants is far less known.According to recent research,serotonin is vital for plant growth,development,and stress responses,achieved through transcriptional and phytohormonal interplay.Specifically,this review addresses critical gaps in the understanding of serotonin's function in plants by examining its biosynthesis,metabolism,and its multifaceted role in mitigating both abiotic stresses(salinity,drought,heat,cold,and heavy metals)as well as biotic challenges(pathogens,pests,and herbivores).As a pivotal player,it engages in a variety of significant cellular and molecular interactions,including those with reactive oxygen and nitrogen species(RONS),and various phytohormones such as auxin,abscisic acid(ABA),salicylic acid(SA),jasmonic acid(JA),ethylene(ET),and cytokinin(CK).Advances in serotonin-related research are anticipated to offer a valuable basis for uncovering the regulatory pathways by which serotonin impacts the resilience of crops against abiotic stress.展开更多
Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monocul...Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monoculm1(MOC1),a critical TF controlling tiller number and plant height in rice,positively regulates rice cold tolerance at the seedling stage.We found that OsMPK4,a mitogen-activated protein kinase,phosphorylates and stabilizes MOC1 under cold stress.Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress.Notably,we found that the OsbZIP79-MOC1-OsNAC5 complex activates several cold-responsive genes,including Dehydration-responsive element-binding factor 1G(OsDREB1G),to confer rice cold tolerance.Haplotype analysis of the OsDREB1G promoter in>10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance.Collectively,our work demonstrates a pivotal role of the OsMPK4-OsbZIP79-MOC1-OsNAC5-OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.展开更多
Drought is the leading environmental threat affecting crop productivity,and plants have evolved a series of mechanisms to adapt to drought stress.The FT-interacting proteins(FTIPs)and phosphatidylethanola mine.binding...Drought is the leading environmental threat affecting crop productivity,and plants have evolved a series of mechanisms to adapt to drought stress.The FT-interacting proteins(FTIPs)and phosphatidylethanola mine.binding proteins(PEBPs)play key roles in developmental processes,whereas their roles in the regulation of stress response are still largely unknown.Here,we report that OsFTIP1 negatively regulates drought response in rice.We showed that OsFTIP1 interacts with rice MOTHER OF FT AND TFL1(OsMFT1),a PEBP that promotes rice tolerance to drought treatment.Further studies discovered that OsMFT1 interacts with two key drought-related transcription factors,OsbZIP66 and OsMYB26,regulating their binding capacity on drought-related genes and thereby enhancing drought toleranee in rice.Interestingly,we found that OsFTIP1 impedes the nucleocytoplasmic translocation of OsMFT1,implying that dynamic modulation of drought-responsive genes by the OsMFT1-OsMYB26 and OsMFT1-OsbZIP66 complexes is integral to OsFTIP1-modulated nuclear accumulation of OsMFT1.Our findings also suggest that OsMFT1 might act as a hitherto unknown nucleocytoplasmic trafficking signal that regulates drought tolerance in rice in response to environmental signals.展开更多
Melatonin(Mel)has previously been reported to effectively alleviate nitrogen-limitation(N-L)stress and thus increase nitrogen-use efficiency(NUE)in several plants,but the underlying mechanism remains obscure.Here,we r...Melatonin(Mel)has previously been reported to effectively alleviate nitrogen-limitation(N-L)stress and thus increase nitrogen-use efficiency(NUE)in several plants,but the underlying mechanism remains obscure.Here,we revealed that OsbZIP79(BASIC LEUCINE ZIPPER 79)is transcriptionally activated under N-L conditions,and its expression is further enhanced by exogenous Mel.By the combined use of omics,genetics,and biological techniques,we revealed that the OsbZIP79–OsABI5(ABSCISIC ACID INSENSITIVE 5)module stimulated regulation of reactive oxygen species(ROS)homeostasis and the uptake and metabolismof nitrogen under conditions of indoor nitrogen limitation(1/16 normal level).OsbZIP79 activated the transcription of OsABI5,and OsABI5 then bound to the promoters of target genes,including genes involved in ROS homeostasis and nitrogen metabolism,activating their transcription.This module was also indispensable for upregulation of several other genes involved in abscisic acid catabolism,nitrogen uptake,and assimilation under N-L and Mel treatment,although these genes were not directly transactivated by OsABI5.Field experiments demonstrated that Mel significantly improved rice growth under low nitrogen(L-N,half the normal level)by the same mechanism revealed in the nitrogen-limitation study.Mel application produced a 28.6%yield increase under L-N and thus similar increases in NUE.Also,two OsbZIP79-overexpression lines grown in L-N field plots had significantly higher NUE(+13.7%and+21.2%)than their wild types.Together,our data show that an OsbZIP79–OsABI5 module regulates the rice response to N insufficiency(N limitation or low N),which is important for increasing NUE in rice production.展开更多
基金supported by the Zhejiang Provincial S&T Project on Breeding of Agricultural (Food) Crops (2016C020502)the Major Science and Technology Innovation Program of Hangzhou, China (2015012A09)
文摘Stigma exsertion in male sterile lines of hybrid rice is important for seed yield.In the present study, ZS616 [Oryza sativa subsp.Xian(indica)], a male sterile line with a stigma exsertion rate(SER) as high as 94.5%, was crossed to DS552, a japonica line with almost no exserted stigmas.F3 plants with extremely low and high SER were sequenced to identify SER-associated quantitative trait loci(QTL).A major QTL for SER, qSER-3.1, was identified along with other QTL on chromosome 3 in a 3.9 Mb region.A total of 307 nonsynonymous single-nucleotide polymorphisms(SNPs) and 27 frame-shift insertion/deletions(InDels)differentiating ZS616 and DS552 were identified in the region containing qSER-3.1.Most SNPs(294) and InDels(25) were excluded after further analysis because they were shared by ZS616 and low(<2.0%) SER accessions in the Huazhong Agricultural University(HAU) core rice collection.Association analysis using the full HAU collection identified a 17-bp InDel in OS03 G0689400 as the most likely causal genetic variant underlying qSER-3.1.ZS616-type accessions(n = 54, with the 17-bp insertion) in the HAU collection had minimum(16.5%)and mean(39.6%) SERs significantly greater than those(n = 424) without the insertion(with minimum and mean SERs of 0.2% and 20.6%, respectively).Thus, this study identified a major QTL for stigma exsertion and revealed the mutation in a candidate gene for the QTL.
基金supported by the National Nuclear Energy Exploitation Program-Nuclear Irradiation for Crop Improvement and Insect Eradication and the International Atomic Energy Agency(IAEA)Coordination Research Project(No.D23020-23042),China.
文摘Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host–pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research.
基金supported by the National Science Foundation of China(32460077)the“Nanhai New Star”Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362)+1 种基金the Scientific Research Fund of Zhejiang Provincial Education Department(Y202353681)the Initial Scientific Research Fund of Hainan Institute,Zhejiang University(0201-6602-A12203).
文摘Serotonin(5-hydroxytryptamine(5-HT))is a pineal hormone and a secondary metabolite related to various hormonal and physiological functions at the organ,tissue,and cellular levels.It is considered increasingly important in regulating animal behavior,but the function of serotonin in plants is far less known.According to recent research,serotonin is vital for plant growth,development,and stress responses,achieved through transcriptional and phytohormonal interplay.Specifically,this review addresses critical gaps in the understanding of serotonin's function in plants by examining its biosynthesis,metabolism,and its multifaceted role in mitigating both abiotic stresses(salinity,drought,heat,cold,and heavy metals)as well as biotic challenges(pathogens,pests,and herbivores).As a pivotal player,it engages in a variety of significant cellular and molecular interactions,including those with reactive oxygen and nitrogen species(RONS),and various phytohormones such as auxin,abscisic acid(ABA),salicylic acid(SA),jasmonic acid(JA),ethylene(ET),and cytokinin(CK).Advances in serotonin-related research are anticipated to offer a valuable basis for uncovering the regulatory pathways by which serotonin impacts the resilience of crops against abiotic stress.
基金supported by the National Natural Science Foundation of China(32570362,32460077,and 32570749)the Hainan Province Science and Technology Special Fund(ZDYF2025XDNY093)+4 种基金the Hainan Provincial Natural Science Foundation of China(325RC799)the Nanhai New Star Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362)the Research Startup Funding from Hainan Institute of Zhejiang University(0201-6602-A12203)the Open Project Program of the State Key Laboratory of Rice Biology and Breeding(20240101)the Open Project Program of Jiaxing Academy of Agricultural Sciences(202405).
文摘Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance,but the transcription factors(TFs)-centered regulatory networks remain largely unclear.In this study,we show that Monoculm1(MOC1),a critical TF controlling tiller number and plant height in rice,positively regulates rice cold tolerance at the seedling stage.We found that OsMPK4,a mitogen-activated protein kinase,phosphorylates and stabilizes MOC1 under cold stress.Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress.Notably,we found that the OsbZIP79-MOC1-OsNAC5 complex activates several cold-responsive genes,including Dehydration-responsive element-binding factor 1G(OsDREB1G),to confer rice cold tolerance.Haplotype analysis of the OsDREB1G promoter in>10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance.Collectively,our work demonstrates a pivotal role of the OsMPK4-OsbZIP79-MOC1-OsNAC5-OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.
基金supported by the National Natural Science Foundation of China(32070209 and 32000213)the Zhejiang Provincial Natural Science Foundation of China(LR21C130001 and LQ21C020003)+2 种基金the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01002)the Key Research and Development Program of Zhejiang(2020C02002)the Singapore National Research Foundation Investigatorship Program(NRF-NRFI2016-02).
文摘Drought is the leading environmental threat affecting crop productivity,and plants have evolved a series of mechanisms to adapt to drought stress.The FT-interacting proteins(FTIPs)and phosphatidylethanola mine.binding proteins(PEBPs)play key roles in developmental processes,whereas their roles in the regulation of stress response are still largely unknown.Here,we report that OsFTIP1 negatively regulates drought response in rice.We showed that OsFTIP1 interacts with rice MOTHER OF FT AND TFL1(OsMFT1),a PEBP that promotes rice tolerance to drought treatment.Further studies discovered that OsMFT1 interacts with two key drought-related transcription factors,OsbZIP66 and OsMYB26,regulating their binding capacity on drought-related genes and thereby enhancing drought toleranee in rice.Interestingly,we found that OsFTIP1 impedes the nucleocytoplasmic translocation of OsMFT1,implying that dynamic modulation of drought-responsive genes by the OsMFT1-OsMYB26 and OsMFT1-OsbZIP66 complexes is integral to OsFTIP1-modulated nuclear accumulation of OsMFT1.Our findings also suggest that OsMFT1 might act as a hitherto unknown nucleocytoplasmic trafficking signal that regulates drought tolerance in rice in response to environmental signals.
基金supported by the Initial Scientific Research Fund of Hainan Institute,Zhejiang University(0201-6602-A12203)the National Nuclear Energy Exploitation Program–Nuclear Irradiation for Crop Improvement and Insect Eradication(D23032-23042)+3 种基金the Program of Breeding New Varieties of Major Crops of Zhejiang Province(2021C02063)the PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City(HSPHDSRF-2023-04-018)the Fundamental Research Funds for the Central Universities(226-2022-00012)the China Postdoctoral Science Foundation(2020M680078),the Open Project Program of the State Key Laboratory of Rice Biology(20200104),and the Agriculture Research System of Shanghai,China(202203).
文摘Melatonin(Mel)has previously been reported to effectively alleviate nitrogen-limitation(N-L)stress and thus increase nitrogen-use efficiency(NUE)in several plants,but the underlying mechanism remains obscure.Here,we revealed that OsbZIP79(BASIC LEUCINE ZIPPER 79)is transcriptionally activated under N-L conditions,and its expression is further enhanced by exogenous Mel.By the combined use of omics,genetics,and biological techniques,we revealed that the OsbZIP79–OsABI5(ABSCISIC ACID INSENSITIVE 5)module stimulated regulation of reactive oxygen species(ROS)homeostasis and the uptake and metabolismof nitrogen under conditions of indoor nitrogen limitation(1/16 normal level).OsbZIP79 activated the transcription of OsABI5,and OsABI5 then bound to the promoters of target genes,including genes involved in ROS homeostasis and nitrogen metabolism,activating their transcription.This module was also indispensable for upregulation of several other genes involved in abscisic acid catabolism,nitrogen uptake,and assimilation under N-L and Mel treatment,although these genes were not directly transactivated by OsABI5.Field experiments demonstrated that Mel significantly improved rice growth under low nitrogen(L-N,half the normal level)by the same mechanism revealed in the nitrogen-limitation study.Mel application produced a 28.6%yield increase under L-N and thus similar increases in NUE.Also,two OsbZIP79-overexpression lines grown in L-N field plots had significantly higher NUE(+13.7%and+21.2%)than their wild types.Together,our data show that an OsbZIP79–OsABI5 module regulates the rice response to N insufficiency(N limitation or low N),which is important for increasing NUE in rice production.
