Ferroptosis is a newly recognized form of programmed cell death characterized by iron overload-dependent lipid peroxidation.These pathological phenomena are often observed in neurodegenerative diseases.Aging is an irr...Ferroptosis is a newly recognized form of programmed cell death characterized by iron overload-dependent lipid peroxidation.These pathological phenomena are often observed in neurodegenerative diseases.Aging is an irreversible process characterized by the deterioration of tissue and cell function.It has been shown to contribute to neurodegenerative diseases and increase susceptibility to ferroptosis.Therefore,ferroptosis may be involved in the progression of neurodegenerative diseases as a pathogenic factor,and aging is the common catalyst of both processes.The purpose of this review is to elucidate the latest progress on the mechanisms related to ferroptosis in neurodegenerative diseases,including iron overload,lipid peroxidation,antioxidant defense,cell membrane repair,and the regulation of autophagy and transcription factors.We also explored the relationship between ferroptosis and aging and reported that aging can induce ferroptosis by increasing iron overload,enhancing lipid peroxidation,and exacerbating autophagy disorders.Since ferroptosis is a pathogenic factor in neurodegenerative diseases,we screened gene bank databases and found that many genes associated with ferroptosis and neurodegenerative diseases overlap.Additionally,genes related to both the peroxidation pathway and ferroptosis are enriched.Ferroptosis occurs under conditions of age-related iron accumulation and lipid enrichment,as well as due to disorders in autophagy levels and transcription factors.Furthermore,in various neurodegenerative diseases,specific pathological changes or products can also contribute to the occurrence of ferroptosis.Finally,based on animal studies and clinical trials involving ferroptosis inhibitors,physical therapies,stem cell treatments,and exosome therapies in neurodegenerative diseases,it has been found that inhibiting ferroptosis can effectively reverse neurological dysfunction and cognitive impairment associated with these conditions.However,given various limitations,the conclusions of some animal studies and clinical trials have not been ideal,indicating that further large-scale research is necessary.Taken together,ferroptosis induces aging-related neurodegenerative diseases and neuronal cell death,triggering disease onset and progression.Ferroptosis inhibitors,physical therapies,stem cell treatments,and exosome therapies show great potential for inhibiting ferroptosis in neurodegenerative disease.展开更多
Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight ...Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight junctions.Since renal pathophysiology is associated with gut barrier integrity,we hypothesized that A.muciniphila may have preventive effects on AKI.We established a lipopolysaccharide(LPS)-induced AKI mouse model to evaluate the effects of A.muciniphila.Our findings showed that pretreatment with A.muciniphila significantly attenuated kidney injury,as evidenced by reduced serum creatinine and urea nitrogen levels,alongside decreased tubular necrosis and apoptosis.A.muciniphila preserved intestinal barrier integrity and induced marked shifts in gut microbial ecology and the metabolome.A.muciniphila notably induced an increase in the relative abundance of the phylum Proteobacteria while decreasing in that of the phylum Bacteroidetes.At the genus level,Prevotella,Faecalibaculum,Moraxella,and Lactobacillus were more abundant in A.muciniphilapretreated mice.Metabolomic analysis revealed that A.muciniphila altered the gut metabolome,with changes involving pathways such as tyrosine metabolism,alanine/aspartate/glutamate homeostasis,cancer-related carbon flux,and GABAergic synaptic signaling.In conclusion,our findings indicate that A.muciniphila exerts renoprotective effects by modulating the gut-kidney axis,thereby establishing a foundation for future studies to explore the connection between gut microbiota and AKI.展开更多
Recent progress in inverted perovskite solar cells(i PSCs)highlights the critical role of interface engineering between the charge transport layer and perovskite.Self-assembled monolayers(SAM)on transparent conductive...Recent progress in inverted perovskite solar cells(i PSCs)highlights the critical role of interface engineering between the charge transport layer and perovskite.Self-assembled monolayers(SAM)on transparent conductive oxide electrodes serve effectively as hole transport layers,though challenges such as energy mismatches and surface inhomogeneities remain.Here,a blended self-assembled monolayer of(2-(9H-carbazol-9-yl)ethyl)phosphonic acid(2PACz)and(4-(3,6-Dimethyl-9H-carbazol-9-yl)butyl)phosphonic acid(Me-4PACz)is developed,offering improved surface potential uniformity and interfacial energy alignment compared to individual SAMs.Interactions between the SAMs and ionic species are investigated with simulation analysis conducted,revealing the elimination of interfacial energy barriers through precise energy-level tuning.This strategy enables wide-bandgap(1.67 e V)perovskite solar cells with inverted structures with over 24%efficiency,an open-circuit voltage(V_(oc))of 1.268 V,and a certified fill factor(FF)of 86.8%,leading to a certified efficiency of 23.42%.The approach also enables high-efficiency semi-transparent devices and a mechanically stacked four-terminal perovskite/silicon tandem solar cell reaching 30.97%efficiency.展开更多
BACKGROUND Gastritis cystica profunda(GCP)is a rare submucosal gastric lesion characterized by the extension of cystically dilated gastric mucosal glands into or below the muscularis mucosa,often due to various underl...BACKGROUND Gastritis cystica profunda(GCP)is a rare submucosal gastric lesion characterized by the extension of cystically dilated gastric mucosal glands into or below the muscularis mucosa,often due to various underlying causes.Typically,asymptomatic or associated with mild symptoms,GCP is most commonly discovered incidentally during surgery or endoscopy.To the best of our knowledge,this is the first documented case of GCP accompanied by acute inflammation and abscess formation.CASE SUMMARY A 37-year-old woman presented with upper abdominal pain.Laboratory tests showed elevated inflammatory markers and carbohydrate antigen 19-9 levels.Gastroscopy revealed a submucosal bulge.Based on enhanced computed tomography findings,an ectopic pancreas with cyst was suspected.Clinically,we decided to perform distal gastrectomy.Postoperative pathology confirmed that the patient had GCP complicated by acute inflammation and abscess formation.The patient had an uneventful postoperative recovery.CONCLUSION This case provides information on new complications of GCP and emphasizes the diagnostic value of enhanced computed tomography.展开更多
The taxonomic composition and function of endophytic microbial communities in plants are strongly influenced by environmental changes. The impact of nitrogen (N) fertilization on the diversity of microbial communities...The taxonomic composition and function of endophytic microbial communities in plants are strongly influenced by environmental changes. The impact of nitrogen (N) fertilization on the diversity of microbial communities in the underground parts of plants that are tolerant of barren environments throughout their growth period remains largely unexplored. Here, by high-throughput sequencing technology, an experiment was performed at the Pailou Experimental Station of Nanjing Agricultural University, where the soil is a yellow-brown soil, to analyze fluctuations in the microbial communities of Jerusalem artichoke (Helianthus tuberosus L.) underground parts in two treatments, application of N, P, and K fertilizers (NPK) and application of only P and K fertilizers (PK), from March 2018 to January 2019. Total, nitrate, and ammonium N contents of soil and Jerusalem artichoke were significantly decreased in the PK treatment. The bacterial community, particularly Acinetobacter, dominated throughout the growth period, peaking in November and accounting for 88.9% and 87.3%, respectively, in the NPK and PK treatments. The absence of N fertilizer (i.e., NP treatment) had minimal effect on the bacterial community composition compared to the fungal community composition. The α-diversity of bacteria in tubers was not substantial, while that in roots was dramatically reduced in July and September in the NP treatment. Notably, the α-diversity of fungal species exhibited more pronounced seasonal variations than that of bacteria under both fertilizer conditions. The lack of N fertilizer lessened the complexity of bacterial network structure and reduced bacterial community similarity (β-diversity) in different months. Interestingly, the β-diversity and network structure of fungal community in the underground tissues of Jerusalem artichoke showed less sensitivity to N fertilizer compared to those of bacterial community. This study improves the understanding of the dynamic response of endophytic microorganisms to N fertilizer application in the underground parts of Jerusalem artichoke tolerant to barren environments.展开更多
Spinal cord injury is characte rized by diffe rent aetiologies,complex pathogenesis,and diverse pathological changes.Current treatments are not ideal,and prognosis is generally poor.After spinal cord injury,neurons di...Spinal cord injury is characte rized by diffe rent aetiologies,complex pathogenesis,and diverse pathological changes.Current treatments are not ideal,and prognosis is generally poor.After spinal cord injury,neurons die due to various forms of cell death.Among them,fe rroptosis causes dysfunction after spinal cord injury,and no existing traditional treatments have been indicated to block its occurrence.Meanwhile,emerging therapies using mesenchymal stem cells,extracellular vesicles,and transcranial magnetic stimulation therapy are promising for reve rsing spinal co rd neuronal ferroptosis after spinal cord injury.However,no definitive studies have demonstrated the effectiveness of these approaches.This review summarizes the existing research on the mechanisms of ferroptosis;fe rroptosis after spinal cord injury;treatment of spinal cord injury with mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation;and treatment of ferroptosis using mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation.Inhibiting ferroptosis can promote the reversal of neurological dysfunction after spinal cord injury.In addition,mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation can reve rse adverse outcomes of spinal cord injury and regulate ferroptosis-related fa ctors.Thus,it can be inferred that mesenchymal stem cells,extracellular vesicles,and transcranial magnetic stimulation have the potential to inhibit fe rroptosis after spinal cord injury.This review serves as a reference for future research to confirm these conclusions.展开更多
Under the Paris agreement, China has committed to reducing CO_2 emissions by 60%–65% per unit of GDP by 2030.Since CO_2 emissions from coal-fired power plants currently account for over 30% of the total carbon emissi...Under the Paris agreement, China has committed to reducing CO_2 emissions by 60%–65% per unit of GDP by 2030.Since CO_2 emissions from coal-fired power plants currently account for over 30% of the total carbon emissions in China, it will be necessary to mitigate at least some of these emissions to achieve this goal. Studies by the International Energy Agency(IEA) indicate CCS technology has the potential to contribute 14% of global emission reductions, followed by 40% of higher energy efficiency and 35% of renewable energy, which is considered as the most promising technology to significantly reduce carbon emissions for current coal-fired power plants.Moreover, the announcement of a Chinese national carbon trading market in late 2017 signals an opportunity for the commercial deployment of CO_2 capture technologies.Currently, the only commercially demonstrated technology for post-combustion CO_2 capture technology from power plants is solvent-based absorption. While commercially viable, the costs of deploying this technology are high. This has motivated efforts to develop more affordable alternatives, including advanced solvents, membranes,and sorbent capture systems. Of these approaches, advanced solvents have received the most attention in terms of research and demonstration. In contrast, sorbent capture technology has less attention, despite its potential for much lower energy consumption due to the absence of water in the sorbent. This paper reviews recent progress in the development of sorbent materials modified by amine functionalities with an emphasis on material characterization methods and the effects of operating conditions on performance. The main problems and challenges that need to be overcome to improve the competitiveness of sorbent-based capture technologies are discussed.展开更多
Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:Th...Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:The TCMSP database was employed to collect and screen the active ingredients of the Chinese herb contained in the XBJ injection.The GeneCards database and STRING database were applied to collect and expand the targets of COVID-19 and compare and screen the related targets of COVID-19 by XBJ injection.Cytoscape was employed to build a network connecting Chinese medicine,compounds,targets,disease,and topology analysis was performed via the Network Analyzer to screen the key ingredients and targets.The software of Schrödinger molecular docking was used to verify the binding activity of the key ingredients of XBJ injection and the key targets of COVID-19.Metascape platform and DAVID database were utilized to conduct Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis on the key targets of COVID-19 treated by XBJ injection.Results:Eight key compounds and 15 key targets were screened and verified by molecular docking;these key compounds included luteolin,quercetin,baicalein,and kaempferol.The key targets included DPP4,AR,ESR1,CALM1,and protein kinase 1.Gene Ontology analysis involved an apoptosis and hypoxia reaction and the changes in blood vessel morphology.Kyoto Encyclopedia of Genes and Genomes analysis involved signaling pathways of hypoxia inducible factor-1,VEGF,and PI3K/AKT/NF-κB.Conclusion:The mechanism of XBJ injection when used to treat COVID-19 should be further investigated as the key compounds in XBJ regulated the expression of key targets such as protein kinase 1,VEGF-A,B-cell lymphoma-2,and TNF,which affected the COVID-19 receptors such as angiotensin-converting enzyme 2 and signaling pathways like hypoxia inducible factor-1,PI3K-Akt,and NF-κB,which alleviated the inflammation,respiratory distress,and hypoxia caused by COVID-19 infection.展开更多
To effectively solve the agglomeration problems in the solid state reaction process,pre-adding glucose is adopted to the synthesis of Li Fe PO4/C energy materials using Fe–P waste slag. The average particle grain siz...To effectively solve the agglomeration problems in the solid state reaction process,pre-adding glucose is adopted to the synthesis of Li Fe PO4/C energy materials using Fe–P waste slag. The average particle grain size of Li FeP O4/C decreases,and the impurities in Li Fe PO4/C composites reduce to a great extent. It makes great sense to the mass industrial production. The optimum synthesis conditions determined in this work are based on the orthogonal experiments. The samples synthesized in a scale of 500 g exhibit high purity,excellent electrochemical performance,high reaction activity,good reversibility,and low polarization level.The discharge capacities are 145,134,117,and 102 m Ah/g at the current densities of 0.1 C,0.2 C,0.5 C and1 C,respectively. This work puts forward a practical suggestion for mass producing environmental benign and low cost Li FeP O4/C as cathode materials of lithium ion batteries.展开更多
Objective：To investigate a possible association of LMP2/LMP7 genes with chronic hepatitis C virus（HCV） infection,and to assess whether LMP2/LMP7 genes could influence the outcomes of HCV infection among drug users....Objective：To investigate a possible association of LMP2/LMP7 genes with chronic hepatitis C virus（HCV） infection,and to assess whether LMP2/LMP7 genes could influence the outcomes of HCV infection among drug users.Methods：Genomic DNAs of 362 anti-HCV sero-positive drug users and 225 control drug users were extracted from the peripheral blood leukocytes.The sero-positive patients were divided into those who had persistent infection and those who had spontaneously cleared the infection.Polymorphisms of LMP genes were determined by PCR combined with restriction fragment length polymorphism（RFLP）.Results：The distribution of LMP2 genotypes among the control,persistent infection and spontaneous clearance groups were not different.However,the LMP7 codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in control group（OR=1.75,95%CI=1.06～2.90;OR=3.16,95%CI=1.23-8.12;OR=1.94,95%CI=1.21-3.12,respectively）.Similarly,the frequencies of the codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in the spontaneous clearance group（OR=1.64,95%CI=1.04-2.57;OR=2.40,95%CI=1.09-5.28;OR=1.76,95%CI=1.152.69,respectively）.Stratified analysis indicated that combined genotype Gln/Lys＋Lys/Lys of the LMP7 gene was related to an increasing susceptibility to HCV infection（OR=1.91,95%CI=1.02-3.55;OR=2.19,95%CI=1.243.89;OR=1.91,95%CI=1.05-3.48,OR=2.86,95%CI=1.41-5.78,respectively）and the risk of persistent HCV infection（OR=1.94,95%CI=1.12-3.34;OR=2.02,95%CI=1.21-3.38;OR=1.78,95%CI=1.12-2.85,OR=2.23,95%CI=1.09-4.58,respectively）among30-year-old,males,the injection drug user（IDU）subjects and/or the shorter duration drug users（≤5 y）.Conclusion：These results suggest that polymorphism of the LMP7 gene may have an influence on the outcomes of HCV infection,and is one of the factors accounting for the genetic susceptibility to HCV infection among drug users.展开更多
Objective: To evaluate the ctinicat efficacy of music therapy in the treatment of children with cerebrat patsy by systematic review and meta-anatysis. Methods: A comprehensive search of China Knowledge Network, Wan fa...Objective: To evaluate the ctinicat efficacy of music therapy in the treatment of children with cerebrat patsy by systematic review and meta-anatysis. Methods: A comprehensive search of China Knowledge Network, Wan fang, VIP database and foreign databases PubMed and Cochrane Library was conducted from the setup of database to March 2019. After reading and screening them carefully, 14 qualified literates were got. Then extracted relevant data, and evaluated the quality and risk bias with Revman5.3.3 software. Results: There is no significant difference between music therapy in improving ctinostatism and the ability of rowing over (P > 0.05). However, music therapy has significant difference in improving sitting, running, and jumping movements, and it can atso relieve muscte spasm, improve muscte tension and stabilize children's emotions in the course of treatment and the P values of which were att tess than 0.05. Conclusion: The music therapy has a certain effect for the treatment of pediatric cerebrat patsy, especiatty in improving children's gross and fine motor abilities, which has a certain ctinicat guiding significance for rehabilitation of children with cerebrat patsy. Key words: Pediatric cerebrat patsy, Music therapy, Meta-anatysisy Ctinicat efficacyObjective: To evaluate the ctinicat efficacy of music therapy in the treatment of children with cerebrat patsy by systematic review and meta-anatysis. Methods: A comprehensive search of China Knowledge Network, Wan fang, VIP database and foreign databases PubMed and Cochrane Library was conducted from the setup of database to March 2019. After reading and screening them carefuLy, 14 qualified [iterates were got. Then extracted relevant data, and evaluated the quality and risk bias with Revman5.3.3 software. Results: There is no significant difference between music therapy in improving ctinostatism and the ability of rowing over (P > 0.05). However, music therapy has significant difference in improving sitting, running, and jumping movements, and it can atso relieve muscte spasm, improve muscte tension and stabilize children's emotions in the course of treatment and the P values of which were att tess than 0.05. Conclusion: The music therapy has a certain effect for the treatment of pediatric cerebrat patsy, especiatty in improving children's gross and fine motor abilities, which has a certain ctinicat guiding significance for rehabilitation of children with cerebrat patsy.展开更多
Objective:To evaluate the clinical effects of bloodletting cupping therapy(BCT)for patients with postherpetic neuralgia.Methods:We comprehensively searched four Chinese databases(Wanfang,China National Knowledge Infra...Objective:To evaluate the clinical effects of bloodletting cupping therapy(BCT)for patients with postherpetic neuralgia.Methods:We comprehensively searched four Chinese databases(Wanfang,China National Knowledge Infrastructure,VIP Database for Chinese Technical Periodicals,China Biology Medicine disc)and foreign databases(Pubmed,Cochrane Library).The retrieval time was from the build-up time to May 2018.RevMan 5.3 software was used for statistical analysis.Results:We finally obtained 18 eligible studies.Compared with routine western medicine,BCT can significantly improve PHN(OR=7.45,95%CI,3.36-16.51,Z=4.95,P<0.01).BCT plus other methods of acupuncture with fire needle and filiform needle has significant therapeutic effect(OR=5.54,95%CI,3.01-10.19,P<0.01),BCT plus routine western medicine has significant therapeutic effect(OR=4.99,95%CI,2.73-9.11,P<0.00001).Conclusion:It showed that the therapeutic effects of BCT,BCT plus other acupuncture methods with fire needle and filiform needle,and BCT combined with western medicine are better than those treated with single western medicine.It is effective to treat postherpetic neuralgia with Bloodletting Cupping Therapy.展开更多
Objective:To systematically evaluate the clinical efficacy and safety of blood-letting puncture and cupping in the treatment of periarthritis of shoulder.Methods:We electronically searched databases including CNKI,VIP...Objective:To systematically evaluate the clinical efficacy and safety of blood-letting puncture and cupping in the treatment of periarthritis of shoulder.Methods:We electronically searched databases including CNKI,VIP,PubMed,WanFang Data,EMbase Cochrane Library and to collect randomized controlled trials(RCTs)and quasi-randomized controlled trials about blood-letting puncture and cupping for periarthritis of shoulder and evaluated the quality of the documents collected by the Cochrane collaboration,provided by Review Manager 5 software for statistical analysis.Then the quality of the included trials was assessed using the Jadad scale and GRADE system was used to score the quality and grade recommendation.Results:Thirty RCTs involving 2556 patients were included.The results of meta-analysis showed that:Total effective rate OR=6.21[OR=6.21,95%CI(4.69,8.21),P<0.001].The results of GRADE system evaluation showed that the level of evidence was B,and the recommendation level was weak recommendation.Conclusion:Blood-letting puncture and cupping shows better effects than other treatments and preferably security in the treatment of periarthritis of shoulder.We do not find any serious adverse reaction reports.Due to limited quality of the included studies,more high quality studies are needed to verify the above conclusion.展开更多
[Objectives]To study the optimum sowing density of quinoa in Chengde region.[Methods]Yanli No.2 cultivated by Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences was taken as test material.In ...[Objectives]To study the optimum sowing density of quinoa in Chengde region.[Methods]Yanli No.2 cultivated by Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences was taken as test material.In Chengde region,single-factor density test design was used to study the effects of sowing on agronomic characters and yield of quinoa.[Results]Quinoa had the highest comprehensive yield when row spacing was 30 cm and plant spacing was 25 cm.[Conclusions]The research could provide theoretical basis for quinoa planting in Chengde region.展开更多
Diabetic nephropathy is one of the most serious complications of diabetes mellitus. In the early stage, edema and proteinuria are the main clinical manifestations. In the later stage, glomerulosclerosis and interstiti...Diabetic nephropathy is one of the most serious complications of diabetes mellitus. In the early stage, edema and proteinuria are the main clinical manifestations. In the later stage, glomerulosclerosis and interstitial fibrosis will occur. And the prognosis is poor. Nowadays, traditional Chinese medicine has a remarkable curative effect in the treatment of diabetic nephropathy. There are more and more studies on the treatment of diabetic nephropathy with traditional Chinese medicine, but most of them focus on the syndromes of diabetic nephropathy, which are short of in-depth research and summary on the mechanism of Chinese herbal prescriptions. In this paper, the traditional Chinese medicine inheritance support system and network pharmacology BATMAN-TCM software were used to collect and analyze the relevant literature. It was found that the core compatibility of Shanzhuyu, Fuling and Shuyu in the treatment of diabetic nephropathy is closely related to the signal transduction pathway and target of diabetic nephropathy, and has a positive effect on the improvement of clinical symptoms such as proteinuria, glycometabolism disorder, edema, etc. This paper explores the core compatibility of Shanzhuyu, Fuling and Shuyu on diabetic nephropathy, in order to provide reference for clinical treatment.展开更多
Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical applicati...Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical application.Methods:The main active components of Huangqi and Shanzhuyu were discerned through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID).The targets related to diabetic nephropathy(DN)were obtained using Genecards,Therapeutic Target Database(TTD),and National Center for Biotechnology Information(NCBI)Gene.Subsequently,protein-protein interaction(PPI)networks were constructed with Cytoscape 3.7.2 and the STRING database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed to analyze the intersection of targets via Database for Annotation,Visualization,and Integrated Discovery(DAVID)6.8.Built on the above analysis,we made a“Chinese medicine-chemical composition-target gene-disease”network.Results:Twenty-one active components were predicted from the Huangqi and Shanzhuyu herb pair,such as jaranol,mandenol and sitosterol.These components were applied to 41 targets mainly involved in many biological processes such as the PI3K-Akt signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway,regulation of sodium ion transport and steroid-binding.Conclusion:This study proposes the network pharmacology method and identifies the potent combination therapeutic mechanism of Huangqi and Shanzhuyu for diabetic nephropathy(DN)through multiple targets and routes,this strategy will lay a good foundation for further in-depth study of the mechanism of action.展开更多
Ventricular premature beat,a kind of arrhythmia,is one of common diseases which not only decreases the quality of life but also has the danger of death.The pathogenesis in Traditional Chinese Medicine is that the inju...Ventricular premature beat,a kind of arrhythmia,is one of common diseases which not only decreases the quality of life but also has the danger of death.The pathogenesis in Traditional Chinese Medicine is that the injury of heart and kidney is the essence and the block of solid evil such as phlegm and blood stasis is the manifestation.The teacher,Li Yuan,has worked in clinic for decades and considered that the important pathogenesis of the VP beat is“heat transformed from Yang”,which cannot be ignored as it is on the influence of diverse factors and can be observed in different symptoms and different treatment states of the disease.展开更多
基金supported by the National Natural Science Foundation of China,No.81101462(to LZ)the National Key Research and Development Program of China,No.2020YFC2005700(to LZ)+2 种基金the Natural Science Foundation of Liaoning Province,China,Nos.201602875,2019-KF-01-06(both LZ)Key Research and Development Program of Liaoning Province,China,No.2024JH2/102500021(to LZ)the Natural Science Foundation of Liaoning Province,China,No.2022-YGJC-56(to SS).
文摘Ferroptosis is a newly recognized form of programmed cell death characterized by iron overload-dependent lipid peroxidation.These pathological phenomena are often observed in neurodegenerative diseases.Aging is an irreversible process characterized by the deterioration of tissue and cell function.It has been shown to contribute to neurodegenerative diseases and increase susceptibility to ferroptosis.Therefore,ferroptosis may be involved in the progression of neurodegenerative diseases as a pathogenic factor,and aging is the common catalyst of both processes.The purpose of this review is to elucidate the latest progress on the mechanisms related to ferroptosis in neurodegenerative diseases,including iron overload,lipid peroxidation,antioxidant defense,cell membrane repair,and the regulation of autophagy and transcription factors.We also explored the relationship between ferroptosis and aging and reported that aging can induce ferroptosis by increasing iron overload,enhancing lipid peroxidation,and exacerbating autophagy disorders.Since ferroptosis is a pathogenic factor in neurodegenerative diseases,we screened gene bank databases and found that many genes associated with ferroptosis and neurodegenerative diseases overlap.Additionally,genes related to both the peroxidation pathway and ferroptosis are enriched.Ferroptosis occurs under conditions of age-related iron accumulation and lipid enrichment,as well as due to disorders in autophagy levels and transcription factors.Furthermore,in various neurodegenerative diseases,specific pathological changes or products can also contribute to the occurrence of ferroptosis.Finally,based on animal studies and clinical trials involving ferroptosis inhibitors,physical therapies,stem cell treatments,and exosome therapies in neurodegenerative diseases,it has been found that inhibiting ferroptosis can effectively reverse neurological dysfunction and cognitive impairment associated with these conditions.However,given various limitations,the conclusions of some animal studies and clinical trials have not been ideal,indicating that further large-scale research is necessary.Taken together,ferroptosis induces aging-related neurodegenerative diseases and neuronal cell death,triggering disease onset and progression.Ferroptosis inhibitors,physical therapies,stem cell treatments,and exosome therapies show great potential for inhibiting ferroptosis in neurodegenerative disease.
基金funded by the National Natural Science Foundation of China(Grant No.82470766 to H.M.)the Jiangsu Provincial Medical Key Discipline(Laboratory)Cultivation Unit(Grant No.JSDW202206 to C.X.)the First Affiliated Hospital of Nanjing Medical University Clinical Capacity Enhancement Project(Grant No.JSPH-MC-2022-18 to C.X.).
文摘Acute kidney injury(AKI)is a critical condition with limited effective therapies.Akkermansia muciniphila(A.muciniphila)is a probiotic with multiple beneficial effects,including the regulation of epithelial cell tight junctions.Since renal pathophysiology is associated with gut barrier integrity,we hypothesized that A.muciniphila may have preventive effects on AKI.We established a lipopolysaccharide(LPS)-induced AKI mouse model to evaluate the effects of A.muciniphila.Our findings showed that pretreatment with A.muciniphila significantly attenuated kidney injury,as evidenced by reduced serum creatinine and urea nitrogen levels,alongside decreased tubular necrosis and apoptosis.A.muciniphila preserved intestinal barrier integrity and induced marked shifts in gut microbial ecology and the metabolome.A.muciniphila notably induced an increase in the relative abundance of the phylum Proteobacteria while decreasing in that of the phylum Bacteroidetes.At the genus level,Prevotella,Faecalibaculum,Moraxella,and Lactobacillus were more abundant in A.muciniphilapretreated mice.Metabolomic analysis revealed that A.muciniphila altered the gut metabolome,with changes involving pathways such as tyrosine metabolism,alanine/aspartate/glutamate homeostasis,cancer-related carbon flux,and GABAergic synaptic signaling.In conclusion,our findings indicate that A.muciniphila exerts renoprotective effects by modulating the gut-kidney axis,thereby establishing a foundation for future studies to explore the connection between gut microbiota and AKI.
文摘Recent progress in inverted perovskite solar cells(i PSCs)highlights the critical role of interface engineering between the charge transport layer and perovskite.Self-assembled monolayers(SAM)on transparent conductive oxide electrodes serve effectively as hole transport layers,though challenges such as energy mismatches and surface inhomogeneities remain.Here,a blended self-assembled monolayer of(2-(9H-carbazol-9-yl)ethyl)phosphonic acid(2PACz)and(4-(3,6-Dimethyl-9H-carbazol-9-yl)butyl)phosphonic acid(Me-4PACz)is developed,offering improved surface potential uniformity and interfacial energy alignment compared to individual SAMs.Interactions between the SAMs and ionic species are investigated with simulation analysis conducted,revealing the elimination of interfacial energy barriers through precise energy-level tuning.This strategy enables wide-bandgap(1.67 e V)perovskite solar cells with inverted structures with over 24%efficiency,an open-circuit voltage(V_(oc))of 1.268 V,and a certified fill factor(FF)of 86.8%,leading to a certified efficiency of 23.42%.The approach also enables high-efficiency semi-transparent devices and a mechanically stacked four-terminal perovskite/silicon tandem solar cell reaching 30.97%efficiency.
文摘BACKGROUND Gastritis cystica profunda(GCP)is a rare submucosal gastric lesion characterized by the extension of cystically dilated gastric mucosal glands into or below the muscularis mucosa,often due to various underlying causes.Typically,asymptomatic or associated with mild symptoms,GCP is most commonly discovered incidentally during surgery or endoscopy.To the best of our knowledge,this is the first documented case of GCP accompanied by acute inflammation and abscess formation.CASE SUMMARY A 37-year-old woman presented with upper abdominal pain.Laboratory tests showed elevated inflammatory markers and carbohydrate antigen 19-9 levels.Gastroscopy revealed a submucosal bulge.Based on enhanced computed tomography findings,an ectopic pancreas with cyst was suspected.Clinically,we decided to perform distal gastrectomy.Postoperative pathology confirmed that the patient had GCP complicated by acute inflammation and abscess formation.The patient had an uneventful postoperative recovery.CONCLUSION This case provides information on new complications of GCP and emphasizes the diagnostic value of enhanced computed tomography.
基金supported by the Foreign Experts Project of China(No.G2022145002 L)the National Natural Science Foundation of China(No.31571702)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(No.809001).
文摘The taxonomic composition and function of endophytic microbial communities in plants are strongly influenced by environmental changes. The impact of nitrogen (N) fertilization on the diversity of microbial communities in the underground parts of plants that are tolerant of barren environments throughout their growth period remains largely unexplored. Here, by high-throughput sequencing technology, an experiment was performed at the Pailou Experimental Station of Nanjing Agricultural University, where the soil is a yellow-brown soil, to analyze fluctuations in the microbial communities of Jerusalem artichoke (Helianthus tuberosus L.) underground parts in two treatments, application of N, P, and K fertilizers (NPK) and application of only P and K fertilizers (PK), from March 2018 to January 2019. Total, nitrate, and ammonium N contents of soil and Jerusalem artichoke were significantly decreased in the PK treatment. The bacterial community, particularly Acinetobacter, dominated throughout the growth period, peaking in November and accounting for 88.9% and 87.3%, respectively, in the NPK and PK treatments. The absence of N fertilizer (i.e., NP treatment) had minimal effect on the bacterial community composition compared to the fungal community composition. The α-diversity of bacteria in tubers was not substantial, while that in roots was dramatically reduced in July and September in the NP treatment. Notably, the α-diversity of fungal species exhibited more pronounced seasonal variations than that of bacteria under both fertilizer conditions. The lack of N fertilizer lessened the complexity of bacterial network structure and reduced bacterial community similarity (β-diversity) in different months. Interestingly, the β-diversity and network structure of fungal community in the underground tissues of Jerusalem artichoke showed less sensitivity to N fertilizer compared to those of bacterial community. This study improves the understanding of the dynamic response of endophytic microorganisms to N fertilizer application in the underground parts of Jerusalem artichoke tolerant to barren environments.
基金supported by a grant from funded by the National Natural Science Foundation of China (Youth Program),No.81101462Natural Science Foundation of Liaoning Province,Nos.2016028 75 and 2019-KF-01-06+1 种基金Liaoning Provincial Public Welfare Science,No.2016003001University of China Medical University Discipline Development Project,No.112-3110119071 (all to LXZ)。
文摘Spinal cord injury is characte rized by diffe rent aetiologies,complex pathogenesis,and diverse pathological changes.Current treatments are not ideal,and prognosis is generally poor.After spinal cord injury,neurons die due to various forms of cell death.Among them,fe rroptosis causes dysfunction after spinal cord injury,and no existing traditional treatments have been indicated to block its occurrence.Meanwhile,emerging therapies using mesenchymal stem cells,extracellular vesicles,and transcranial magnetic stimulation therapy are promising for reve rsing spinal co rd neuronal ferroptosis after spinal cord injury.However,no definitive studies have demonstrated the effectiveness of these approaches.This review summarizes the existing research on the mechanisms of ferroptosis;fe rroptosis after spinal cord injury;treatment of spinal cord injury with mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation;and treatment of ferroptosis using mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation.Inhibiting ferroptosis can promote the reversal of neurological dysfunction after spinal cord injury.In addition,mesenchymal stem cells,extracellular vesicles,and transc ranial magnetic stimulation can reve rse adverse outcomes of spinal cord injury and regulate ferroptosis-related fa ctors.Thus,it can be inferred that mesenchymal stem cells,extracellular vesicles,and transcranial magnetic stimulation have the potential to inhibit fe rroptosis after spinal cord injury.This review serves as a reference for future research to confirm these conclusions.
基金Supported by the National Key Research and Development Program of China(2017YFB0603301)
文摘Under the Paris agreement, China has committed to reducing CO_2 emissions by 60%–65% per unit of GDP by 2030.Since CO_2 emissions from coal-fired power plants currently account for over 30% of the total carbon emissions in China, it will be necessary to mitigate at least some of these emissions to achieve this goal. Studies by the International Energy Agency(IEA) indicate CCS technology has the potential to contribute 14% of global emission reductions, followed by 40% of higher energy efficiency and 35% of renewable energy, which is considered as the most promising technology to significantly reduce carbon emissions for current coal-fired power plants.Moreover, the announcement of a Chinese national carbon trading market in late 2017 signals an opportunity for the commercial deployment of CO_2 capture technologies.Currently, the only commercially demonstrated technology for post-combustion CO_2 capture technology from power plants is solvent-based absorption. While commercially viable, the costs of deploying this technology are high. This has motivated efforts to develop more affordable alternatives, including advanced solvents, membranes,and sorbent capture systems. Of these approaches, advanced solvents have received the most attention in terms of research and demonstration. In contrast, sorbent capture technology has less attention, despite its potential for much lower energy consumption due to the absence of water in the sorbent. This paper reviews recent progress in the development of sorbent materials modified by amine functionalities with an emphasis on material characterization methods and the effects of operating conditions on performance. The main problems and challenges that need to be overcome to improve the competitiveness of sorbent-based capture technologies are discussed.
基金This study was supported by the Foundation of Health Commission of Hebei Province(20190123)the Natural Science Foundation of Hebei Province of China(H2018201179).
文摘Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:The TCMSP database was employed to collect and screen the active ingredients of the Chinese herb contained in the XBJ injection.The GeneCards database and STRING database were applied to collect and expand the targets of COVID-19 and compare and screen the related targets of COVID-19 by XBJ injection.Cytoscape was employed to build a network connecting Chinese medicine,compounds,targets,disease,and topology analysis was performed via the Network Analyzer to screen the key ingredients and targets.The software of Schrödinger molecular docking was used to verify the binding activity of the key ingredients of XBJ injection and the key targets of COVID-19.Metascape platform and DAVID database were utilized to conduct Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis on the key targets of COVID-19 treated by XBJ injection.Results:Eight key compounds and 15 key targets were screened and verified by molecular docking;these key compounds included luteolin,quercetin,baicalein,and kaempferol.The key targets included DPP4,AR,ESR1,CALM1,and protein kinase 1.Gene Ontology analysis involved an apoptosis and hypoxia reaction and the changes in blood vessel morphology.Kyoto Encyclopedia of Genes and Genomes analysis involved signaling pathways of hypoxia inducible factor-1,VEGF,and PI3K/AKT/NF-κB.Conclusion:The mechanism of XBJ injection when used to treat COVID-19 should be further investigated as the key compounds in XBJ regulated the expression of key targets such as protein kinase 1,VEGF-A,B-cell lymphoma-2,and TNF,which affected the COVID-19 receptors such as angiotensin-converting enzyme 2 and signaling pathways like hypoxia inducible factor-1,PI3K-Akt,and NF-κB,which alleviated the inflammation,respiratory distress,and hypoxia caused by COVID-19 infection.
基金supported by the National Science Foundation of China(grant no.21206099)
文摘To effectively solve the agglomeration problems in the solid state reaction process,pre-adding glucose is adopted to the synthesis of Li Fe PO4/C energy materials using Fe–P waste slag. The average particle grain size of Li FeP O4/C decreases,and the impurities in Li Fe PO4/C composites reduce to a great extent. It makes great sense to the mass industrial production. The optimum synthesis conditions determined in this work are based on the orthogonal experiments. The samples synthesized in a scale of 500 g exhibit high purity,excellent electrochemical performance,high reaction activity,good reversibility,and low polarization level.The discharge capacities are 145,134,117,and 102 m Ah/g at the current densities of 0.1 C,0.2 C,0.5 C and1 C,respectively. This work puts forward a practical suggestion for mass producing environmental benign and low cost Li FeP O4/C as cathode materials of lithium ion batteries.
基金supported by the National Mega-project of Science Research Project(No.2008ZX10002-013 and No.2009ZX1004-904)
文摘Objective：To investigate a possible association of LMP2/LMP7 genes with chronic hepatitis C virus（HCV） infection,and to assess whether LMP2/LMP7 genes could influence the outcomes of HCV infection among drug users.Methods：Genomic DNAs of 362 anti-HCV sero-positive drug users and 225 control drug users were extracted from the peripheral blood leukocytes.The sero-positive patients were divided into those who had persistent infection and those who had spontaneously cleared the infection.Polymorphisms of LMP genes were determined by PCR combined with restriction fragment length polymorphism（RFLP）.Results：The distribution of LMP2 genotypes among the control,persistent infection and spontaneous clearance groups were not different.However,the LMP7 codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in control group（OR=1.75,95%CI=1.06～2.90;OR=3.16,95%CI=1.23-8.12;OR=1.94,95%CI=1.21-3.12,respectively）.Similarly,the frequencies of the codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in the spontaneous clearance group（OR=1.64,95%CI=1.04-2.57;OR=2.40,95%CI=1.09-5.28;OR=1.76,95%CI=1.152.69,respectively）.Stratified analysis indicated that combined genotype Gln/Lys＋Lys/Lys of the LMP7 gene was related to an increasing susceptibility to HCV infection（OR=1.91,95%CI=1.02-3.55;OR=2.19,95%CI=1.243.89;OR=1.91,95%CI=1.05-3.48,OR=2.86,95%CI=1.41-5.78,respectively）and the risk of persistent HCV infection（OR=1.94,95%CI=1.12-3.34;OR=2.02,95%CI=1.21-3.38;OR=1.78,95%CI=1.12-2.85,OR=2.23,95%CI=1.09-4.58,respectively）among30-year-old,males,the injection drug user（IDU）subjects and/or the shorter duration drug users（≤5 y）.Conclusion：These results suggest that polymorphism of the LMP7 gene may have an influence on the outcomes of HCV infection,and is one of the factors accounting for the genetic susceptibility to HCV infection among drug users.
文摘Objective: To evaluate the ctinicat efficacy of music therapy in the treatment of children with cerebrat patsy by systematic review and meta-anatysis. Methods: A comprehensive search of China Knowledge Network, Wan fang, VIP database and foreign databases PubMed and Cochrane Library was conducted from the setup of database to March 2019. After reading and screening them carefully, 14 qualified literates were got. Then extracted relevant data, and evaluated the quality and risk bias with Revman5.3.3 software. Results: There is no significant difference between music therapy in improving ctinostatism and the ability of rowing over (P > 0.05). However, music therapy has significant difference in improving sitting, running, and jumping movements, and it can atso relieve muscte spasm, improve muscte tension and stabilize children's emotions in the course of treatment and the P values of which were att tess than 0.05. Conclusion: The music therapy has a certain effect for the treatment of pediatric cerebrat patsy, especiatty in improving children's gross and fine motor abilities, which has a certain ctinicat guiding significance for rehabilitation of children with cerebrat patsy. Key words: Pediatric cerebrat patsy, Music therapy, Meta-anatysisy Ctinicat efficacyObjective: To evaluate the ctinicat efficacy of music therapy in the treatment of children with cerebrat patsy by systematic review and meta-anatysis. Methods: A comprehensive search of China Knowledge Network, Wan fang, VIP database and foreign databases PubMed and Cochrane Library was conducted from the setup of database to March 2019. After reading and screening them carefuLy, 14 qualified [iterates were got. Then extracted relevant data, and evaluated the quality and risk bias with Revman5.3.3 software. Results: There is no significant difference between music therapy in improving ctinostatism and the ability of rowing over (P > 0.05). However, music therapy has significant difference in improving sitting, running, and jumping movements, and it can atso relieve muscte spasm, improve muscte tension and stabilize children's emotions in the course of treatment and the P values of which were att tess than 0.05. Conclusion: The music therapy has a certain effect for the treatment of pediatric cerebrat patsy, especiatty in improving children's gross and fine motor abilities, which has a certain ctinicat guiding significance for rehabilitation of children with cerebrat patsy.
基金the National Natural Science Foundation of Hebei(No.H2018201179).
文摘Objective:To evaluate the clinical effects of bloodletting cupping therapy(BCT)for patients with postherpetic neuralgia.Methods:We comprehensively searched four Chinese databases(Wanfang,China National Knowledge Infrastructure,VIP Database for Chinese Technical Periodicals,China Biology Medicine disc)and foreign databases(Pubmed,Cochrane Library).The retrieval time was from the build-up time to May 2018.RevMan 5.3 software was used for statistical analysis.Results:We finally obtained 18 eligible studies.Compared with routine western medicine,BCT can significantly improve PHN(OR=7.45,95%CI,3.36-16.51,Z=4.95,P<0.01).BCT plus other methods of acupuncture with fire needle and filiform needle has significant therapeutic effect(OR=5.54,95%CI,3.01-10.19,P<0.01),BCT plus routine western medicine has significant therapeutic effect(OR=4.99,95%CI,2.73-9.11,P<0.00001).Conclusion:It showed that the therapeutic effects of BCT,BCT plus other acupuncture methods with fire needle and filiform needle,and BCT combined with western medicine are better than those treated with single western medicine.It is effective to treat postherpetic neuralgia with Bloodletting Cupping Therapy.
基金Youth fund for natural science research of institutions of higher learning in Hebei province(No.QN2016077)Hebei province medical science research key project(No.20160388)Natural science foundation of hebei province(No.H2018201179).
文摘Objective:To systematically evaluate the clinical efficacy and safety of blood-letting puncture and cupping in the treatment of periarthritis of shoulder.Methods:We electronically searched databases including CNKI,VIP,PubMed,WanFang Data,EMbase Cochrane Library and to collect randomized controlled trials(RCTs)and quasi-randomized controlled trials about blood-letting puncture and cupping for periarthritis of shoulder and evaluated the quality of the documents collected by the Cochrane collaboration,provided by Review Manager 5 software for statistical analysis.Then the quality of the included trials was assessed using the Jadad scale and GRADE system was used to score the quality and grade recommendation.Results:Thirty RCTs involving 2556 patients were included.The results of meta-analysis showed that:Total effective rate OR=6.21[OR=6.21,95%CI(4.69,8.21),P<0.001].The results of GRADE system evaluation showed that the level of evidence was B,and the recommendation level was weak recommendation.Conclusion:Blood-letting puncture and cupping shows better effects than other treatments and preferably security in the treatment of periarthritis of shoulder.We do not find any serious adverse reaction reports.Due to limited quality of the included studies,more high quality studies are needed to verify the above conclusion.
基金Supported by Key R&D Projects in Hebei Province(19227527D)Science and Technology Innovation Team Project of Hebei Coarse Grain and Soybean Seed Industry。
文摘[Objectives]To study the optimum sowing density of quinoa in Chengde region.[Methods]Yanli No.2 cultivated by Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences was taken as test material.In Chengde region,single-factor density test design was used to study the effects of sowing on agronomic characters and yield of quinoa.[Results]Quinoa had the highest comprehensive yield when row spacing was 30 cm and plant spacing was 25 cm.[Conclusions]The research could provide theoretical basis for quinoa planting in Chengde region.
文摘Diabetic nephropathy is one of the most serious complications of diabetes mellitus. In the early stage, edema and proteinuria are the main clinical manifestations. In the later stage, glomerulosclerosis and interstitial fibrosis will occur. And the prognosis is poor. Nowadays, traditional Chinese medicine has a remarkable curative effect in the treatment of diabetic nephropathy. There are more and more studies on the treatment of diabetic nephropathy with traditional Chinese medicine, but most of them focus on the syndromes of diabetic nephropathy, which are short of in-depth research and summary on the mechanism of Chinese herbal prescriptions. In this paper, the traditional Chinese medicine inheritance support system and network pharmacology BATMAN-TCM software were used to collect and analyze the relevant literature. It was found that the core compatibility of Shanzhuyu, Fuling and Shuyu in the treatment of diabetic nephropathy is closely related to the signal transduction pathway and target of diabetic nephropathy, and has a positive effect on the improvement of clinical symptoms such as proteinuria, glycometabolism disorder, edema, etc. This paper explores the core compatibility of Shanzhuyu, Fuling and Shuyu on diabetic nephropathy, in order to provide reference for clinical treatment.
文摘Background:To explore the action mechanism of Huangqi(Radix Astragali)and Shanzhuyu(Fructus Corni)in the treatment of diabetic nephropathy based on network pharmacology,aiming to provide a basis for clinical application.Methods:The main active components of Huangqi and Shanzhuyu were discerned through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID).The targets related to diabetic nephropathy(DN)were obtained using Genecards,Therapeutic Target Database(TTD),and National Center for Biotechnology Information(NCBI)Gene.Subsequently,protein-protein interaction(PPI)networks were constructed with Cytoscape 3.7.2 and the STRING database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed to analyze the intersection of targets via Database for Annotation,Visualization,and Integrated Discovery(DAVID)6.8.Built on the above analysis,we made a“Chinese medicine-chemical composition-target gene-disease”network.Results:Twenty-one active components were predicted from the Huangqi and Shanzhuyu herb pair,such as jaranol,mandenol and sitosterol.These components were applied to 41 targets mainly involved in many biological processes such as the PI3K-Akt signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway,regulation of sodium ion transport and steroid-binding.Conclusion:This study proposes the network pharmacology method and identifies the potent combination therapeutic mechanism of Huangqi and Shanzhuyu for diabetic nephropathy(DN)through multiple targets and routes,this strategy will lay a good foundation for further in-depth study of the mechanism of action.
文摘Ventricular premature beat,a kind of arrhythmia,is one of common diseases which not only decreases the quality of life but also has the danger of death.The pathogenesis in Traditional Chinese Medicine is that the injury of heart and kidney is the essence and the block of solid evil such as phlegm and blood stasis is the manifestation.The teacher,Li Yuan,has worked in clinic for decades and considered that the important pathogenesis of the VP beat is“heat transformed from Yang”,which cannot be ignored as it is on the influence of diverse factors and can be observed in different symptoms and different treatment states of the disease.
