OBJECTIVE:To assess the long-term effectiveness of Huangqi(Radix Astragali Mongolici,HQ)-based Traditional Chinese Medicine(TCM)in the treatment of diabetic peripheral neuropathy(DPN).METHODS:Nine databases were searc...OBJECTIVE:To assess the long-term effectiveness of Huangqi(Radix Astragali Mongolici,HQ)-based Traditional Chinese Medicine(TCM)in the treatment of diabetic peripheral neuropathy(DPN).METHODS:Nine databases were searched to retrieve available randomized controlled trials that compared HQbased TCM and Western Medicines in the treatment of DPN.The methodological quality of the included studies was assessed using the Cochrane bias risk tool,and RevMan 5.4 was used for data analysis.The effect estimates of interest were risk ratio(RR),mean difference(MD)or standardized mean difference(SMD)with 95%confidence interval(CI).RESULTS:The results from 48 available studies assessing 3759 patients demonstrated that cases administered HQ-based TCM[RR=1.30,95%CI(1.21,1.40),P<0.00001]or HQ-based TCM combined with Western Medicines[RR=1.25,95%CI(1.19,1.31),P<0.00001]exhibited higher total efficacy rates than individuals who received Western Medicine alone.The results showed that the HQ-based TCM group had decreased Toronto Clinical Scoring System scores[MD=-1.50,95%CI(-1.83,-1.17),P<0.00001],and reduced serum interleukin 6[SMD=-0.57,95%CI(-0.87,-0.27),P=0.0002]and tumor necrosis factors-αlevels[SMD=-0.60,95%CI(-0.95,-0.25),P=0.0009].In addition,both HQ-based TCM and HQbased TCM combined with Western Medicine increased nerve conduction velocity and decreased glycaemia compared with Western Medicine alone.In terms of blood lipids,oxidative stress and adverse drug reactions,there were no significant differences between the HQ-based TCM groups and the Western Medicine control group.CONCLUSION:The current Meta-analysis revealed that HQ-based TCM yields higher efficacy and safety than Western Medicine alone for the treatment of DPN,although further well-designed RCTs are required to validate these findings.展开更多
目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。...目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。采用流感病毒滴鼻方法建立病毒感染模型,根据给药量制备相应浓度药物并灌胃给药,正常组和模型组给予同体积生理盐水。采用RT-PCR、免疫组化法检测给药后1、3、5、7天各时间点各组小鼠Toll样受体4(toll like receptors 4,TLR4)和核转录因子κB(nuclear factor-κB,NF-κB)蛋白及其mRNA动态表达情况。结果:YQS、YPF及YPS在不同时点均能不同程度阻断流感小鼠肺组织中TLR4、NF-κB蛋白及其mRNA的高表达,并且YPS组表达最低,差异有统计学意义(P<0.05)。结论:双表法对流感病毒感染小鼠的防治作用与抑制流感病毒感染小鼠肺组织TLR4/NF-κB信号通路过度活化有关。展开更多
目的探讨RGC32(response gene to complement 32)在子宫颈鳞状细胞癌及鳞状上皮内病变组织中的表达以及临床意义。方法采用免疫组化法分别检测正常宫颈、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、鳞状细胞癌组织(SCC)中RG...目的探讨RGC32(response gene to complement 32)在子宫颈鳞状细胞癌及鳞状上皮内病变组织中的表达以及临床意义。方法采用免疫组化法分别检测正常宫颈、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、鳞状细胞癌组织(SCC)中RGC32蛋白的表达情况,并且分析RGC32表达与宫颈鳞状细胞癌临床特征的关系。结果宫颈鳞状细胞癌及高度鳞状上皮内病变中RGC32的阳性率显著高于低度鳞状上皮内病变及正常宫颈(P<0.05)。RGC32蛋白在有淋巴结转移组阳性率显著高于无淋巴结转移组(P=0.014)。结论 RGC32在癌前病变及SCC中的阳性率显著高于非癌前病变,同时在发生淋巴结转移组阳性率显著高于无淋巴结转移组,提示RGC32可能参与了宫颈癌的发生、发展过程。展开更多
文摘OBJECTIVE:To assess the long-term effectiveness of Huangqi(Radix Astragali Mongolici,HQ)-based Traditional Chinese Medicine(TCM)in the treatment of diabetic peripheral neuropathy(DPN).METHODS:Nine databases were searched to retrieve available randomized controlled trials that compared HQbased TCM and Western Medicines in the treatment of DPN.The methodological quality of the included studies was assessed using the Cochrane bias risk tool,and RevMan 5.4 was used for data analysis.The effect estimates of interest were risk ratio(RR),mean difference(MD)or standardized mean difference(SMD)with 95%confidence interval(CI).RESULTS:The results from 48 available studies assessing 3759 patients demonstrated that cases administered HQ-based TCM[RR=1.30,95%CI(1.21,1.40),P<0.00001]or HQ-based TCM combined with Western Medicines[RR=1.25,95%CI(1.19,1.31),P<0.00001]exhibited higher total efficacy rates than individuals who received Western Medicine alone.The results showed that the HQ-based TCM group had decreased Toronto Clinical Scoring System scores[MD=-1.50,95%CI(-1.83,-1.17),P<0.00001],and reduced serum interleukin 6[SMD=-0.57,95%CI(-0.87,-0.27),P=0.0002]and tumor necrosis factors-αlevels[SMD=-0.60,95%CI(-0.95,-0.25),P=0.0009].In addition,both HQ-based TCM and HQbased TCM combined with Western Medicine increased nerve conduction velocity and decreased glycaemia compared with Western Medicine alone.In terms of blood lipids,oxidative stress and adverse drug reactions,there were no significant differences between the HQ-based TCM groups and the Western Medicine control group.CONCLUSION:The current Meta-analysis revealed that HQ-based TCM yields higher efficacy and safety than Western Medicine alone for the treatment of DPN,although further well-designed RCTs are required to validate these findings.
文摘目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。采用流感病毒滴鼻方法建立病毒感染模型,根据给药量制备相应浓度药物并灌胃给药,正常组和模型组给予同体积生理盐水。采用RT-PCR、免疫组化法检测给药后1、3、5、7天各时间点各组小鼠Toll样受体4(toll like receptors 4,TLR4)和核转录因子κB(nuclear factor-κB,NF-κB)蛋白及其mRNA动态表达情况。结果:YQS、YPF及YPS在不同时点均能不同程度阻断流感小鼠肺组织中TLR4、NF-κB蛋白及其mRNA的高表达,并且YPS组表达最低,差异有统计学意义(P<0.05)。结论:双表法对流感病毒感染小鼠的防治作用与抑制流感病毒感染小鼠肺组织TLR4/NF-κB信号通路过度活化有关。
