This study investigates the use of a low-carbon soil stabilizer called SDG,which is made up of granulated blast furnace slag (GGBFS),desulfurization gypsum (DG),and calcium carbide slag (CCS),to solidify the soil.The ...This study investigates the use of a low-carbon soil stabilizer called SDG,which is made up of granulated blast furnace slag (GGBFS),desulfurization gypsum (DG),and calcium carbide slag (CCS),to solidify the soil.The impact of SDG components on the strength and durability of solidified soil was analysed through a series of tests,including unconfined compressive strength,water stability coefficient,water absorption rate,drying-wetting cycles,and shrinkage tests.Furthermore,microstructure characteristics were analysed using X-ray diffraction (XRD) and scanning electron microscopy (SEM).The study shows that the solidified soil has excellent strength and durability when the SDG stabilizer contains 60% GGBGS,10% DG,and 30% CCS.Additionally,increasing the DG content negatively affects the soil's resistance to water.The SDG stabilizer has potential chemical cementitious characteristics and the calcium carbide slag is rich in calcium ions,which undergo an ion exchange reaction with minerals in the soil.These findings offer new ideas for the development of soil stabilizers.展开更多
DGP1(DEEP GREEN PANICLE1)是植物的一种特异性蛋白,具有保守的TIGR01589结构域,在绿色组织中表达。为探究DGP1基因在甘蓝型油菜中的功能,本研究以甘蓝型油菜‘高油酸1号’为材料,克隆得到2个同源拷贝,长度为288 bp,编码95个氨基酸,分...DGP1(DEEP GREEN PANICLE1)是植物的一种特异性蛋白,具有保守的TIGR01589结构域,在绿色组织中表达。为探究DGP1基因在甘蓝型油菜中的功能,本研究以甘蓝型油菜‘高油酸1号’为材料,克隆得到2个同源拷贝,长度为288 bp,编码95个氨基酸,分别命名BnDGP1-1和BnDGP1-2。生物信息学分析结果表明,BnDGP1-1、BnDGP1-2与拟南芥AT3G55240及水稻OsDGP1分子式相近,均为不稳定亲水性非跨膜蛋白,未找到信号肽结构,均属于非分泌蛋白;BnDGP1-1、BnDGP1-2的二级结构和三级结构相吻合。亚细胞定位结果表明,BnDGP1-1、BnDGP1-2均在细胞核中表达。实时荧光定量PCR分析表明,BnDGP1的5个同源拷贝除BnaA05G0066400ZS在花中相对表达最高,其他拷贝表达趋势没有明显差异,均为叶片>茎>角果皮>花的表达模式,而在根和花蕾中几乎不表达。本文构建了植物表达载体pCAMBIA1304-Bn-DGP1-1、pCAMBIA1304-BnDGP1-2以及CRISPR-cas9敲除载体。展开更多
基金Funded by the National Key R&D Program of China (No. 2022YFC3803405)the China State Construction Key Laboratory Project (No. ZJXJ-PT-2022-14)。
文摘This study investigates the use of a low-carbon soil stabilizer called SDG,which is made up of granulated blast furnace slag (GGBFS),desulfurization gypsum (DG),and calcium carbide slag (CCS),to solidify the soil.The impact of SDG components on the strength and durability of solidified soil was analysed through a series of tests,including unconfined compressive strength,water stability coefficient,water absorption rate,drying-wetting cycles,and shrinkage tests.Furthermore,microstructure characteristics were analysed using X-ray diffraction (XRD) and scanning electron microscopy (SEM).The study shows that the solidified soil has excellent strength and durability when the SDG stabilizer contains 60% GGBGS,10% DG,and 30% CCS.Additionally,increasing the DG content negatively affects the soil's resistance to water.The SDG stabilizer has potential chemical cementitious characteristics and the calcium carbide slag is rich in calcium ions,which undergo an ion exchange reaction with minerals in the soil.These findings offer new ideas for the development of soil stabilizers.
文摘DGP1(DEEP GREEN PANICLE1)是植物的一种特异性蛋白,具有保守的TIGR01589结构域,在绿色组织中表达。为探究DGP1基因在甘蓝型油菜中的功能,本研究以甘蓝型油菜‘高油酸1号’为材料,克隆得到2个同源拷贝,长度为288 bp,编码95个氨基酸,分别命名BnDGP1-1和BnDGP1-2。生物信息学分析结果表明,BnDGP1-1、BnDGP1-2与拟南芥AT3G55240及水稻OsDGP1分子式相近,均为不稳定亲水性非跨膜蛋白,未找到信号肽结构,均属于非分泌蛋白;BnDGP1-1、BnDGP1-2的二级结构和三级结构相吻合。亚细胞定位结果表明,BnDGP1-1、BnDGP1-2均在细胞核中表达。实时荧光定量PCR分析表明,BnDGP1的5个同源拷贝除BnaA05G0066400ZS在花中相对表达最高,其他拷贝表达趋势没有明显差异,均为叶片>茎>角果皮>花的表达模式,而在根和花蕾中几乎不表达。本文构建了植物表达载体pCAMBIA1304-Bn-DGP1-1、pCAMBIA1304-BnDGP1-2以及CRISPR-cas9敲除载体。
