Smart pesticide delivery systems based on stimuli-responsive nanocarriers have attracted considerable attention because of their potential to enhance pesticide efficiency while reducing environmental risks.In this stu...Smart pesticide delivery systems based on stimuli-responsive nanocarriers have attracted considerable attention because of their potential to enhance pesticide efficiency while reducing environmental risks.In this study,a novel p H/glutathione dual-responsive pesticide delivery system was constructed through the synthesis of disulfide-bridged hollow mesoporous organosilica nanospheres(HMONs)via the St??ber method,followed by poly(acrylic acid)(PAA)coating through distillation-precipitation polymerization to form HMONs@PAA nanocomposites.The resulting abamectin-loaded system(Abamectin-HMONs@PAA)demonstrated a 12.73% pesticide loading capacity and significantly improved photostability,retaining twice as much active ingredient as free abamectin after 250 h of UV irradiation(36 W).Release studies revealed p H-and glutathione-dependent characteristics,with cumulative releases in acidic conditions exceeding those in neutral and alkaline environments by 18.66% and 40.98%,respectively,and a 14.2% increase in glutathione-containing solution(0.2 mmol·L^(-1) in 70% ethanol)after 97 h.Bioassays showed superior performance against Plutella xylostella,with a 13.33% reduction in survival rate compared to conventional suspension at equivalent dosage(40 mg·L^(-1)),while maintaining efficacy after extensive rainfall simulation(20 events over 10 days).This study provides a promising approach for developing environmentally responsive nanopesticides with enhanced durability and controlled-release properties,offering significant potential for sustainable crop protection.展开更多
The pulse shape discrimination technique plays a pivotal role in neutron field measurements using organic scintillator detectors,and the particle-type labeling accuracy of the pulse waveform dataset has a significant ...The pulse shape discrimination technique plays a pivotal role in neutron field measurements using organic scintillator detectors,and the particle-type labeling accuracy of the pulse waveform dataset has a significant impact on its performance,especially with the growing use of machine learning methods.In this study,a high-accuracy labeling method for pulse waveform datasets based on the time-of-flight(TOF)filtering method,an improved charge comparison method(CCM),and the coincidence measurement method is proposed.The relationship between the experimental parameters and the chance coincidence proportion in the TOF measurement was derived to reduce contamination from chance coincidences at the experimental level.Based on this,an experiment was conducted to obtain raw data using the^(241)AmBe source,and a piled-up identification algorithm based on reference waveform cross-correlation and differential analysis was designed to filter out piled-up pulses.To improve the labeling accuracy,the CCM was optimized,a simple method of selecting the TOF interval for a lower chance coincidence proportion was proposed,and a low-amplitude pulse waveform dataset construction method based on coincidence measurements was developed.To verify these methods,eight pulse waveform datasets were constructed using different combinations of the proposed approaches.Three neural network structures and a corresponding evaluation parameter were designed to test the quality of these datasets.The results showed that the particle identification performance of the CCM was significantly improved after optimization,with the neutron-to-gamma-ray misidentification rate reduced by more than 35%.The proposed accuracy improvement methods reduced ambiguous identification results from these artificial neural networks by more than 50%.展开更多
Fluorescent probes,with their superior optical properties and labeling versatility,have greatly advanced the visualization of intracellular molecules and subcellular structures.However,poor cytoplasmic delivery,caused...Fluorescent probes,with their superior optical properties and labeling versatility,have greatly advanced the visualization of intracellular molecules and subcellular structures.However,poor cytoplasmic delivery,caused by charge,size,or targeting groups,limits the effective use of many fluorescent probes in live cells.Recently,cell-penetrating peptides(CPPs)have emerged as efficient carriers,offering great potential for the cytoplasmic delivery of fluorescent probes in live cells.This review provides a comprehensive overview of CPPs as vehicles for probe delivery,outlining advances in their development,conjugation chemistries,and intracellular delivery mechanisms.Recent applications in live-cell imaging are highlighted and organized according to major CPP modification strategies,including sequence engineering,cyclization,hybrid design and enhancement by chemical reagents.Finally,the challenges that remain and the future outlook of this rapidly evolvingfield are discussed.展开更多
The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life s...The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.展开更多
Prostate cancer(PCa)is one of the most common malignant tumors in the male genitourinary system,ranking second in incidence worldwide.Traditional Chinese medicine(TCM),as an important component of complementary and al...Prostate cancer(PCa)is one of the most common malignant tumors in the male genitourinary system,ranking second in incidence worldwide.Traditional Chinese medicine(TCM),as an important component of complementary and alternative medicine,shows unique advantages in cancer treatment.Chinese herbal medicine is usually composed of multiple ingredients and involves multiple signaling pathways,which showed function of inducing apoptosis of cancer cells,arresting the cell cycle,inhibiting invasion and metastasis,reducing drug resistance,and regulating immune function.Physical therapy is also an important treatment of TCM.Currently,Physical therapy such as acupuncture or Tai Chi and Qigong are gaining increased recognition in the management of PCa,particularly in addressing issues like urinary incontinence and bone metastasis-related pain.This article reviews the TCM treatment and therapy of PCa,in order to provide new research avenues and treatment options for the treatment of PCa with TCM and improve the quality of life of patients.展开更多
基金financially supported by the Jiangsu Forestry Science and Technology Innovation and Promotion Project(No.LYKJ-Nanjing[2022]02)the Jiangsu Agricultural Science and Technology Innovation Fund(No.CX(23)3090)。
文摘Smart pesticide delivery systems based on stimuli-responsive nanocarriers have attracted considerable attention because of their potential to enhance pesticide efficiency while reducing environmental risks.In this study,a novel p H/glutathione dual-responsive pesticide delivery system was constructed through the synthesis of disulfide-bridged hollow mesoporous organosilica nanospheres(HMONs)via the St??ber method,followed by poly(acrylic acid)(PAA)coating through distillation-precipitation polymerization to form HMONs@PAA nanocomposites.The resulting abamectin-loaded system(Abamectin-HMONs@PAA)demonstrated a 12.73% pesticide loading capacity and significantly improved photostability,retaining twice as much active ingredient as free abamectin after 250 h of UV irradiation(36 W).Release studies revealed p H-and glutathione-dependent characteristics,with cumulative releases in acidic conditions exceeding those in neutral and alkaline environments by 18.66% and 40.98%,respectively,and a 14.2% increase in glutathione-containing solution(0.2 mmol·L^(-1) in 70% ethanol)after 97 h.Bioassays showed superior performance against Plutella xylostella,with a 13.33% reduction in survival rate compared to conventional suspension at equivalent dosage(40 mg·L^(-1)),while maintaining efficacy after extensive rainfall simulation(20 events over 10 days).This study provides a promising approach for developing environmentally responsive nanopesticides with enhanced durability and controlled-release properties,offering significant potential for sustainable crop protection.
基金supported by the National Natural Science Foundation of China(Nos.12375297 and 12105144).
文摘The pulse shape discrimination technique plays a pivotal role in neutron field measurements using organic scintillator detectors,and the particle-type labeling accuracy of the pulse waveform dataset has a significant impact on its performance,especially with the growing use of machine learning methods.In this study,a high-accuracy labeling method for pulse waveform datasets based on the time-of-flight(TOF)filtering method,an improved charge comparison method(CCM),and the coincidence measurement method is proposed.The relationship between the experimental parameters and the chance coincidence proportion in the TOF measurement was derived to reduce contamination from chance coincidences at the experimental level.Based on this,an experiment was conducted to obtain raw data using the^(241)AmBe source,and a piled-up identification algorithm based on reference waveform cross-correlation and differential analysis was designed to filter out piled-up pulses.To improve the labeling accuracy,the CCM was optimized,a simple method of selecting the TOF interval for a lower chance coincidence proportion was proposed,and a low-amplitude pulse waveform dataset construction method based on coincidence measurements was developed.To verify these methods,eight pulse waveform datasets were constructed using different combinations of the proposed approaches.Three neural network structures and a corresponding evaluation parameter were designed to test the quality of these datasets.The results showed that the particle identification performance of the CCM was significantly improved after optimization,with the neutron-to-gamma-ray misidentification rate reduced by more than 35%.The proposed accuracy improvement methods reduced ambiguous identification results from these artificial neural networks by more than 50%.
基金supported by the following grants:National Natural Science Foundation of China(Grant Nos.92354305 and 32271428),National Key R&D Program of China(Grant No.2022YFC3401100)Young Talent Program of Hubei Provincial Health Commission(WJ2025Q037)+1 种基金Interdisciplinary Research Program of HUST(Grant No.2023JCY5045)Director Fund of WNLO.
文摘Fluorescent probes,with their superior optical properties and labeling versatility,have greatly advanced the visualization of intracellular molecules and subcellular structures.However,poor cytoplasmic delivery,caused by charge,size,or targeting groups,limits the effective use of many fluorescent probes in live cells.Recently,cell-penetrating peptides(CPPs)have emerged as efficient carriers,offering great potential for the cytoplasmic delivery of fluorescent probes in live cells.This review provides a comprehensive overview of CPPs as vehicles for probe delivery,outlining advances in their development,conjugation chemistries,and intracellular delivery mechanisms.Recent applications in live-cell imaging are highlighted and organized according to major CPP modification strategies,including sequence engineering,cyclization,hybrid design and enhancement by chemical reagents.Finally,the challenges that remain and the future outlook of this rapidly evolvingfield are discussed.
基金supported by the following grants:National Natural Science Foundation of China(grant nos.92354305,32271428,and 32201132)National Key R&D Program of China(grant no.2022YFC3401100)+1 种基金Fund for Knowledge Innovation of Wuhan Science and Technology Bureau(grant no.2022020801010558)Director Fund of WNLO.
文摘The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field.
基金supported by China Postdoctoral Science Foundation(2022M722674)Peixian Science and Technology Plan Project(P202410)Xuzhou Medical Reserve Talents Project(XWRCHT20220009).
文摘Prostate cancer(PCa)is one of the most common malignant tumors in the male genitourinary system,ranking second in incidence worldwide.Traditional Chinese medicine(TCM),as an important component of complementary and alternative medicine,shows unique advantages in cancer treatment.Chinese herbal medicine is usually composed of multiple ingredients and involves multiple signaling pathways,which showed function of inducing apoptosis of cancer cells,arresting the cell cycle,inhibiting invasion and metastasis,reducing drug resistance,and regulating immune function.Physical therapy is also an important treatment of TCM.Currently,Physical therapy such as acupuncture or Tai Chi and Qigong are gaining increased recognition in the management of PCa,particularly in addressing issues like urinary incontinence and bone metastasis-related pain.This article reviews the TCM treatment and therapy of PCa,in order to provide new research avenues and treatment options for the treatment of PCa with TCM and improve the quality of life of patients.
