Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflam...Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflammatory,antioxidant,and antibacterial conditions.Methods:In this experimental study,extraction,preliminary phytochemical,gas chromatography-mass spectrometry(GC-MS)analysis,and safety profile of the alkaloid-rich fraction of Millettia aboensis(ARFMA)were carried out using standard biochemical methods.The female Wistar rats were given graded dose 100 and 200 mg/kg body weight(bw)of ARFMA leaves which serves as group 3 and 4 respectively.Group 2 rats received 100 mg/kg bw of misoprostol,while group 1 received 5 mL/kg bw as control.Results:The preliminary phytochemical result reveals significant amount of secondary metabolite such as alkaloids,flavonoids and terpenoids.In the GC-MS analysis,total of 13 compounds were detected and identified mainly as aromatic compounds.The ARFMA leaves showed antifertility at 50 and 25%.Meanwhile,200 mg/kg body weight of the ARFMA leaves resulted in 75%abortifacient action.The failure of embryo development is indicated by the ARFMA-treatment,which led to an increase in the percentage of resorption index.When compared to the control(P=0.05),serum progesterone levels significantly(P=0.04,P=0.02 and P=0.01)decreased in anti-implantation as a result of ARFMA-treatment for groups 2,3 and 4 respectively.In comparison to the control,the administration of ARFMA caused as an increase in serum estradiol.The histological architecture of the ovary and uterus disclosed that anti-implantation and abortifacient are consistent with the normal ovarian and uteri histology in the metestrus/diestrus stages of the oestrus cycle in ARFMA-treated groups,except changes which was observed in the uterus of abortifacient histological architecture.Conclusion:The ARFMA exhibits anti-implantation and abortifacient effects on the rats studied,causing a considerable decrease in the number of implants as well as resorption of developed foetuses.展开更多
Dear Editor,Chromosome segregation in eukary-otes relies on intricate interactions between spindle microtubules and centromeres(Liu et al,2020).Recently,using cryo-electron microscopy(cryo-EM)alongside functional anal...Dear Editor,Chromosome segregation in eukary-otes relies on intricate interactions between spindle microtubules and centromeres(Liu et al,2020).Recently,using cryo-electron microscopy(cryo-EM)alongside functional analyses,we and other groups unveiled the molecular basis of how human kinetochore constitutive centromere-associated network(CCAN)interacts with duplex DNA and facilitates accurate chromosome segregation(Pesenti et al,2022;Tian et al.,2022;Yatskevich et al,.2022).While these investigations offer a foundational understanding of the physical and chemical interdependency of CCAN constituents,the functional significance of individual contacts within this complex remains to be fully elucidated.展开更多
文摘Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflammatory,antioxidant,and antibacterial conditions.Methods:In this experimental study,extraction,preliminary phytochemical,gas chromatography-mass spectrometry(GC-MS)analysis,and safety profile of the alkaloid-rich fraction of Millettia aboensis(ARFMA)were carried out using standard biochemical methods.The female Wistar rats were given graded dose 100 and 200 mg/kg body weight(bw)of ARFMA leaves which serves as group 3 and 4 respectively.Group 2 rats received 100 mg/kg bw of misoprostol,while group 1 received 5 mL/kg bw as control.Results:The preliminary phytochemical result reveals significant amount of secondary metabolite such as alkaloids,flavonoids and terpenoids.In the GC-MS analysis,total of 13 compounds were detected and identified mainly as aromatic compounds.The ARFMA leaves showed antifertility at 50 and 25%.Meanwhile,200 mg/kg body weight of the ARFMA leaves resulted in 75%abortifacient action.The failure of embryo development is indicated by the ARFMA-treatment,which led to an increase in the percentage of resorption index.When compared to the control(P=0.05),serum progesterone levels significantly(P=0.04,P=0.02 and P=0.01)decreased in anti-implantation as a result of ARFMA-treatment for groups 2,3 and 4 respectively.In comparison to the control,the administration of ARFMA caused as an increase in serum estradiol.The histological architecture of the ovary and uterus disclosed that anti-implantation and abortifacient are consistent with the normal ovarian and uteri histology in the metestrus/diestrus stages of the oestrus cycle in ARFMA-treated groups,except changes which was observed in the uterus of abortifacient histological architecture.Conclusion:The ARFMA exhibits anti-implantation and abortifacient effects on the rats studied,causing a considerable decrease in the number of implants as well as resorption of developed foetuses.
基金supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,92153302,W2411017,32400593,92253301,22137007,and 92053104)the Plansfor Major Provincial Science&Technology Projects of Anhui Province(202303a0702003).F.A.,L.Z.,X.L.,and X.Y.conceived the project and designed the experiments.O.A.D.and X.G.performed the chemical screening.O.A.D.and F.Y.performed the experiments and analyzed the data.O.A.D.,L.Z.,and X.Y.wrote the manuscript.X.L.and X.Y.supervised the project.
文摘Dear Editor,Chromosome segregation in eukary-otes relies on intricate interactions between spindle microtubules and centromeres(Liu et al,2020).Recently,using cryo-electron microscopy(cryo-EM)alongside functional analyses,we and other groups unveiled the molecular basis of how human kinetochore constitutive centromere-associated network(CCAN)interacts with duplex DNA and facilitates accurate chromosome segregation(Pesenti et al,2022;Tian et al.,2022;Yatskevich et al,.2022).While these investigations offer a foundational understanding of the physical and chemical interdependency of CCAN constituents,the functional significance of individual contacts within this complex remains to be fully elucidated.
