摘要
Dear Editor,Chromosome segregation in eukary-otes relies on intricate interactions between spindle microtubules and centromeres(Liu et al,2020).Recently,using cryo-electron microscopy(cryo-EM)alongside functional analyses,we and other groups unveiled the molecular basis of how human kinetochore constitutive centromere-associated network(CCAN)interacts with duplex DNA and facilitates accurate chromosome segregation(Pesenti et al,2022;Tian et al.,2022;Yatskevich et al,.2022).While these investigations offer a foundational understanding of the physical and chemical interdependency of CCAN constituents,the functional significance of individual contacts within this complex remains to be fully elucidated.
基金
supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,92153302,W2411017,32400593,92253301,22137007,and 92053104)
the Plansfor Major Provincial Science&Technology Projects of Anhui Province(202303a0702003).F.A.,L.Z.,X.L.,and X.Y.conceived the project and designed the experiments.O.A.D.and X.G.performed the chemical screening.O.A.D.and F.Y.performed the experiments and analyzed the data.O.A.D.,L.Z.,and X.Y.wrote the manuscript.X.L.and X.Y.supervised the project.
