采用转录组测序(RNA-seq)技术比较澳洲坚果在7个不同发育阶段的转录组,获得了大量差异表达的Unigene序列。通过GO(Gene Ontology)分类和代谢途径富集性分析,将这些差异表达的Unigene归类于包含脂肪酸生物合成途径在内的406个代谢途径。...采用转录组测序(RNA-seq)技术比较澳洲坚果在7个不同发育阶段的转录组,获得了大量差异表达的Unigene序列。通过GO(Gene Ontology)分类和代谢途径富集性分析,将这些差异表达的Unigene归类于包含脂肪酸生物合成途径在内的406个代谢途径。将Unigene序列在KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中进行比对,获得17个关键酶的同源蛋白序列。本研究通过编码这些同源蛋白的基因在澳洲坚果果仁脂肪酸合成的表达模式进行分析,可为获得澳洲坚果果仁中脂肪酸合成机制的解析提供理论参考,并为进一步的理论研究和澳洲坚果的遗传育种提供潜在的基因资源。展开更多
OBJECTIVE:To evaluate the anti-tumor activity of ethyl acetate fraction(EFA),extracted with ethanol from the root of"Dai-Bai-Jie"in A549 cancer cells and its underlying mechanism.METHODS:"Dai-Bai-Jie&qu...OBJECTIVE:To evaluate the anti-tumor activity of ethyl acetate fraction(EFA),extracted with ethanol from the root of"Dai-Bai-Jie"in A549 cancer cells and its underlying mechanism.METHODS:"Dai-Bai-Jie"was extracted with 95%ethanol-aqueous(DBJ-1),50%ethanol-aqueous(DBJ-2),and water(DBJ-3)by reflux method.95%ethanol-aqueous extract was separated byethyl acetate(EFA)and n-butyl alcohol(DBJ-5),consecutively.The SRB method was used to evaluate the cytotoxic activity.Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell.Western blot was used to detect the apoptosis/related pro-teins expressions.A549 tumor cellsbearing nude mice model was employed to measure the tumor volume,mice weight,and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS:DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5μg/mL,respectively.EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase,and induce apoptosis in A549 tumor cells in vitro.The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53,Bax,Caspase-3,and Caspase-8 proteins expression.EFA also possessed significant anti-tumor efficacy in nude mice,and little toxicity was observed in the host.CONCLUSION:EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest.A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels,and increase in the expression of p53,Caspase-3,and Caspase-8 proteins.展开更多
文摘采用转录组测序(RNA-seq)技术比较澳洲坚果在7个不同发育阶段的转录组,获得了大量差异表达的Unigene序列。通过GO(Gene Ontology)分类和代谢途径富集性分析,将这些差异表达的Unigene归类于包含脂肪酸生物合成途径在内的406个代谢途径。将Unigene序列在KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中进行比对,获得17个关键酶的同源蛋白序列。本研究通过编码这些同源蛋白的基因在澳洲坚果果仁脂肪酸合成的表达模式进行分析,可为获得澳洲坚果果仁中脂肪酸合成机制的解析提供理论参考,并为进一步的理论研究和澳洲坚果的遗传育种提供潜在的基因资源。
基金Supported by the Grant from the Basic Scientific Research Special of the Central Public Welfare Research Institutes of IMPLADChinese Academy of Medical Sciences:Study on anti-lung cancer chemical constituents and mechanism of"Dai-Bai-Jie"(No.YZYN1501)Study on the resources of"Dai-Bai-Jie"(No.YZYN-10-02)
文摘OBJECTIVE:To evaluate the anti-tumor activity of ethyl acetate fraction(EFA),extracted with ethanol from the root of"Dai-Bai-Jie"in A549 cancer cells and its underlying mechanism.METHODS:"Dai-Bai-Jie"was extracted with 95%ethanol-aqueous(DBJ-1),50%ethanol-aqueous(DBJ-2),and water(DBJ-3)by reflux method.95%ethanol-aqueous extract was separated byethyl acetate(EFA)and n-butyl alcohol(DBJ-5),consecutively.The SRB method was used to evaluate the cytotoxic activity.Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell.Western blot was used to detect the apoptosis/related pro-teins expressions.A549 tumor cellsbearing nude mice model was employed to measure the tumor volume,mice weight,and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS:DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5μg/mL,respectively.EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase,and induce apoptosis in A549 tumor cells in vitro.The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53,Bax,Caspase-3,and Caspase-8 proteins expression.EFA also possessed significant anti-tumor efficacy in nude mice,and little toxicity was observed in the host.CONCLUSION:EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest.A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels,and increase in the expression of p53,Caspase-3,and Caspase-8 proteins.
