摘要
为探索芒果败育果与正常果品质性状差异的调控机制,本研究采集‘贵妃’‘金煌’‘台农1号’3个芒果品种花后14、16、18和20周共4个发育时期的败育果和正常果为研究材料。通过Illumina HiSeq平台进行果实的全长转录组测序,结果显示,拼接共得到205230个unigenes,N50为1956 bp,平均长度为1331 bp;通过KEGG、NR、Swiss-Prot、GO、KOG、TrEMBL和Pfam等7个数据库对获得的unigenes进行注释,共有163871个unigenes得到功能注释,占unigenes总数的80%;败育果和正常果之间差异表达基因(DEGs)的GO富集结果表明,4个发育时期的差异表达基因大多富集在生物过程和分子功能的条目上,只有少部分富集在细胞组分的条目上;差异表达基因KEGG富集分析显示,差异表达的基因显著富集的通路涉及磷酸盐、维生素B6和生物素等的代谢通路,吲哚生物碱、花青素、硫代葡萄糖苷、黄酮和类黄酮等次生代谢产物的生物合成通路,以及光合作用等通路。本研究揭示了芒果败育果与正常果的基因差异表达,可为芒果胚败育相关基因挖掘和胚败育分子机制研究提供参考。
The regulatory mechanisms responsible for qualitative phenotypes of the abortive and normal mango fruits were explored.The abortive and normal fruits from three mango varieties,‘Guifei',‘Jinhuang'and‘Tainong No.1',were collected at 14,16,18,and 20 weeks after anthesis.The full-length transcriptome of the fruits was sequenced using the Illumina HiSeq platform,generating 205230 assembled unigenes with an N50 of 1956 bp and an average length of 1331 bp.Seven databases,including KEGG,NR,Swiss-Prot,GO,KOG,TrEMBL and Pfam,were used for annotating 163871 unigenes,accounting for 80%of the total unigenes.The GO erichment showed that most of DEGs in the four developmental stages were enriched in biological process and molecular function,only small partially enriched in cellular component.Additionally,KEGG enrichment suggested that these DEGs are enriched in critical pathways such as phosphonate and phosphinate metabolism,vitamin B6 metabolism,biotin metabolism,indole alkaloid biosynthesis,anthocyanin biosynthesis,glucosinolate biosynthesis,flavone and flavonol biosynthesis,flavonoid biosynthesis,and photosynthesis.Our results revealed differential gene regulation between the abortive and normal mango fruits,which can help identify the genes responsible for embryo abortion.The findings also serve as a basis for further studies on the molecular mechanisms of mango embryo abortion.
作者
牛迎凤
刘紫艳
郑诚
李开雄
柳觐
Niu Yingfeng;Liu Ziyan;Zheng Cheng;Li Kaixiong;Liu Jin(Research Centre for Molecular Biology of Tropical Crops,Yunnan Institute of Tropical Crops,Jinghong,666100)
出处
《分子植物育种》
2026年第3期754-770,共17页
Molecular Plant Breeding
基金
国家自然科学基金地区科学基金项目(32160396)
云南省热带作物科学研究所青年成长基金项目(QNCZ-2020-3)共同资助。
关键词
贵妃
金煌
台农1号
胚败育
转录组
Guifei
Jinhuang
Tainong No.1
Embryo aborted
Transcriptome
