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青藏高原高寒草甸土壤古菌群落对不同放牧强度的响应
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作者 弋维 艾鷖 +2 位作者 吴萌 田黎明 泽让东科 《生物多样性》 北大核心 2025年第1期57-66,共10页
古菌因其独特且多样化的遗传和代谢特征,在全球碳氮循环等过程中发挥着关键作用。放牧家畜啃食、排泄和践踏等行为将影响土壤古菌群落,但关于放牧对高寒草地古菌群落结构及其多样性的影响尚不明确。本研究在青藏高原的高寒草甸进行了为... 古菌因其独特且多样化的遗传和代谢特征,在全球碳氮循环等过程中发挥着关键作用。放牧家畜啃食、排泄和践踏等行为将影响土壤古菌群落,但关于放牧对高寒草地古菌群落结构及其多样性的影响尚不明确。本研究在青藏高原的高寒草甸进行了为期两年的牦牛放牧试验,设置了对照组(禁牧)、轻度放牧(1头牦牛/ha)、中度放牧(2头牦牛/ha)和重度放牧(3头牦牛/ha)4个放牧强度,每个强度设置3个重复。结果表明,土壤古菌群落在各放牧强度之间的α多样性指数均无显著差异,但轻度放牧均增加了土壤古菌群落多样性与丰富度;随着放牧强度的增加,泉古菌门呈现出先减少后增加的趋势,广古菌门和小古菌门相对丰度在轻度放牧中最高,对照组最低。与对照组相比,放牧降低了土壤容重、增加了土壤含水率;重度放牧使土壤全磷降低、有效磷增加;泉古菌门与全磷和全钾呈显著负相关(P<0.05),广古菌门与全磷呈显著正相关(P<0.05)而与全钾呈显著负相关(P<0.05),小古菌门与全磷呈显著正相关(P<0.05)。综上所述,轻度放牧有增加土壤古菌群落丰富度和多样性的趋势,不同土壤古菌群落组成受土壤碳氮影响较大,表明放牧可能通过改变土壤养分进而影响古菌群落结构。研究结果为正确评估放牧对高寒草地生态系统的影响提供了科学基础。 展开更多
关键词 高寒草甸 牦牛 放牧强度 土壤古菌群落 青藏高原 中国
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激光清洗C919飞机铝锂合金蒙皮涂层
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作者 吴猛 李多生 +3 位作者 叶寅 李旭勇 徐雪源 陈加伟 《航空学报》 北大核心 2025年第14期329-344,共16页
激光清洗因环境友好、灵活高效、适应性强在航空领域具有广阔应用。采用纳秒脉冲激光对C919飞机蒙皮铝锂合金(2060)表面CMS-CT-203涂层进行清洗。分析了不同扫描速度、激光功率、脉冲频率、扫描次数组合下对铝锂合金涂层的清洗行为,并... 激光清洗因环境友好、灵活高效、适应性强在航空领域具有广阔应用。采用纳秒脉冲激光对C919飞机蒙皮铝锂合金(2060)表面CMS-CT-203涂层进行清洗。分析了不同扫描速度、激光功率、脉冲频率、扫描次数组合下对铝锂合金涂层的清洗行为,并以去除深度、表面粗糙度为评价指标作极差分析,探究各激光工艺参数对不同评价指标的影响权重。正交优化涂层清洗的激光工艺参数,并对最佳激光工艺参数组合下清洗后的试样进行表征及力学性能分析。结果表明,对于影响清洗效果的权重,扫描次数>扫描速度、激光功率>脉冲频率;最佳激光工艺参数组合为扫描速度900 mm/s、激光功率65 W、脉冲频率140 kHz、扫描次数4次,激光清洗后的试样较好地保持了原试样的表面形貌,同时也发现清洗后试样表面的硬度和抗拉强度略微增加,较好地保持了基材原有力学性能。实现对铝锂合金表面涂层的完全去除并不损伤基材的原力学性能,该研究可为航空表面涂层去除提供参考。 展开更多
关键词 激光清洗 铝锂合金(2060) CMS-CT-203涂层 激光工艺参数 微观形貌 力学性能
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Recycling technologies of spent lithium-ion batteries and future directions:A review 被引量:4
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作者 Xue-song GAO meng wu +5 位作者 Guang-jin ZHAO Kun-hong GU Jia-jia wu Hong-bo ZENG Wen-qing QIN Jun-wei HAN 《Transactions of Nonferrous Metals Society of China》 2025年第1期271-295,共25页
Lithium-ion batteries(LIBs)are the most popular energy storage devices due to their high energy density,high operating voltage,and long cycle life.However,green and effective recycling methods are needed because LIBs ... Lithium-ion batteries(LIBs)are the most popular energy storage devices due to their high energy density,high operating voltage,and long cycle life.However,green and effective recycling methods are needed because LIBs contain heavy metals such as Co,Ni,and Mn and organic compounds inside,which seriously threaten human health and the environment.In this work,we review the current status of spent LIB recycling,discuss the traditional pyrometallurgical and hydrometallurgical recovery processes,and summarize the existing short-process recovery technologies such as salt-assisted roasting,flotation processes,and direct recycling.Finally,we analyze the problems and potential research prospects of the current recycling process,and point out that the multidisciplinary integration of recycling will become the mainstream technology for the development of spent LIBs. 展开更多
关键词 spent lithium battery short-process recycling secondary resources PRETREATMENT metal recovery
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Deciphering and overcoming the high-voltage limitations of halide and sulfide-based all-solid-state lithium batteries 被引量:1
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作者 Xiang Qi Gang wu +5 位作者 meng wu Dabing Li Chao Wang Lei Gao Shichao Zhang Li-Zhen Fan 《Journal of Energy Chemistry》 2025年第4期926-935,共10页
Adopting high-voltage Ni-rich cathodes in halide and sulfide-based all-solid-state lithium batteries(ASSLBs)holds great promise for breaking through the 400 Wh kg^(-1)bottleneck.However,both cell configurations are co... Adopting high-voltage Ni-rich cathodes in halide and sulfide-based all-solid-state lithium batteries(ASSLBs)holds great promise for breaking through the 400 Wh kg^(-1)bottleneck.However,both cell configurations are confronted with intricate interfacial challenges in high-voltage regines(>4.5 V),resulting in inadequate cathode utilization and premature cell degradation.Moreover,contrary to previous studies,coupled with LiNi_(0.85)Co_(0.1)Mn_(0.05)O_(2)cathodes,typical halide(Li_(2)ZrCl_(6))-based cells at 4.5 V feature unlimited interfacial degradation and poor long cycle stability,while typical sulfide(Li_(6)PS_(5)Cl)-based cells feature self-limited interfacial degradation and poor initial cycle stability.Herein,this work addresses the high-voltage limitations of Li_(2)ZrCl_(6)and Li_(6)PS_(5)Cl catholyte-based cells by manipulating electrode mass fraction and tailoring interfacial composition,thereby effectively improving interfacial charge-transfer kinetics and(electro)chemical stability within cathodes.After appropriate interface design,both optimized cells at 4.5 V demonstrate remarkably increased initial discharge capacities(>195 mA h g^(-1)at0.1 C),improved cycle stabilities(>80%after 600 cycles at 0.5 C),and enhanced rate performances(>115 mA h g^(-1)at 1.0 C).This work deepens our understanding of high-voltage applications for halide/sulfide electrolytes and provides generalized interfacial design strategies for advancing high-voltage ASSLBs. 展开更多
关键词 All-solid-state lithium batteries Halide solid electrolytes Sulfide solid electrolytes High voltage Interface design
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CMOS direct conversion X-ray detector coupled with fluorinated liquid 被引量:1
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作者 Shi-Hua Liu Chao-Song Gao +5 位作者 Xin Zhang Xiang-Ming Sun meng wu Zhi-Hui Han Tong Wan Yong-Shuai Ge 《Nuclear Science and Techniques》 2025年第1期59-68,共10页
X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorpho... X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorphous selenium,cadmium telluride zinc,and perovskites,have been utilized in direct conversion X-ray detectors.However,these semiconductor materials are susceptible to temperature-induced performance degradation,crystallization,delamination,uneven lattice growth,radiation damage,and high dark current.This study explores a new approach by coupling an FC40 electronic fluorinated liquid with a specialized high-resolution and high-readout-speed complementary metal-oxide-semiconductor(CMOS)pixel array,specifically the Topmetal II−chip,to fabricate a direct conversion X-ray detector.The fluorinated liquid FC40(molecular formula:C_(21)F_(48)N_(2))is an electronic medium that is minimally affected by temperature and displays no issues with uniform conductivity.It exhibits a low dark current and minimal radiation damage and enables customizable thickness in X-ray absorption.This addresses the limitations inherent in conventional semiconductor-based detectors.In this study,simple X-ray detector imaging tests were conducted,demonstrating the excellent coupling capability between FC40 electronic fluorinated liquid and CMOS chips by the X-ray detector.A spatial resolution of 4.0 lp/mm was measured using a striped line par card,and a relatively clear image of a cockroach was displayed in the digital radiography imaging results.Preliminary test results indicated the feasibility of fabricating an X-ray detector by combining FC40 electronic fluorinated liquid and CMOS chips.Owing to the absence of issues related to chip-material coupling,a high spatial resolution could be achieved by reducing the chip pixel size.This method presents a new avenue for studies on novel liquid-based direct conversion X-ray detectors. 展开更多
关键词 FC40 electronic fluorinated liquid CMOS pixel chip X-ray detector Spatial resolution
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WHOⅡ级脑胶质瘤预后影响因素分析 被引量:13
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作者 彭世义 李艳萍 +3 位作者 陈志萍 涂子为 邬蒙 李国庆 《中国肿瘤临床》 CAS CSCD 北大核心 2018年第8期402-407,共6页
目的:探讨WHOⅡ级脑胶质瘤的预后影响因素。方法:回顾性分析江西省肿瘤医院1997年6月至2015年4月收治的146例经病理诊断为WHOⅡ级脑胶质瘤患者的临床资料,其中星形细胞瘤96例,少枝胶质细胞瘤30例,混合性少枝星形细胞瘤20例;手术全切90例... 目的:探讨WHOⅡ级脑胶质瘤的预后影响因素。方法:回顾性分析江西省肿瘤医院1997年6月至2015年4月收治的146例经病理诊断为WHOⅡ级脑胶质瘤患者的临床资料,其中星形细胞瘤96例,少枝胶质细胞瘤30例,混合性少枝星形细胞瘤20例;手术全切90例,部分切除56例。结果:中位随访时间88个月;5、10年总生存率(overall survival,OS)和无进展生存率(progression free survival,PFS)分别为75.7%、57.4%和60.0%、37.8%;单因素分析显示切除程度、残留大小、性别、年龄、室管膜下区(subventricular zones,SVZ)受侵、瘤周水肿、病灶大小、是否单发为OS的影响因素(均P<0.05);切除程度、残留大小、性别、SVZ受侵、是否单发为PFS的影响因素(均P<0.05),手术全切患者术后放疗延长了PFS(P=0.038);与星形细胞瘤相比,少枝胶质细胞瘤在OS及PFS上均有优势(P=0.040,P=0.049)。多因素分析显示切除程度、瘤周水肿、SVZ受侵为OS的独立影响因素(均P<0.05);切除程度、病灶是否单发是PFS的独立影响因素(均P<0.05)。全组共有60例复发,其中单纯瘤床复发53例,远隔部位复发7例(3例同时伴瘤床复发)。结论:手术切除程度、瘤周水肿、SVZ受侵为影响WHOⅡ级脑胶质瘤患者OS的独立预后因素;全切患者术后放疗能改善PFS;瘤床复发是主要复发模式。 展开更多
关键词 脑胶质瘤 预后 失败模式
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Convergent and divergent signaling pathways in C_(3) rice and C_(4) foxtail millet crops in response to salt stress
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作者 Xinyu Man Sha Tang +7 位作者 Yu meng Yanjia Gong Yanqing Chen meng wu Guanqing Jia Jun Liu Xianmin Diao Xiliu Cheng 《Journal of Integrative Agriculture》 2025年第10期3719-3738,共20页
Salt stress is a global constraint on agricultural production.Therefore,the development of salt tolerant plants has become a current research hotspot.While salt tolerance has evolved more frequently in C_(4) grass lin... Salt stress is a global constraint on agricultural production.Therefore,the development of salt tolerant plants has become a current research hotspot.While salt tolerance has evolved more frequently in C_(4) grass lineages,few studies have explored the molecular bases underlying salt stress tolerance in the C_(4) crop foxtail millet.In this study,we used a multi-pronged approach spanning the omics analyses of transcriptomes and physiological analysis of the C_(3) crop rice and the C_(4) model crop foxtail millet to investigate their responses to salt stress.The results revealed that compared to C_(3) rice,C_(4) foxtail millet has upregulated abscisic acid(ABA)and notably reduced CK biosynthesis and signaling transduction under salt stress.Salt stress in C_(3) rice plants triggered rapid downregulation of photosynthesis related genes,which was coupled with severely reduced net photosynthetic rates.In the salt-treated C_(3) rice and C_(4) foxtail millet,some stress responsive transcription factors(TFs),such as AP2/ERF,WRKY and MYB,underwent strong and distinct transcriptional changes.Based on a weighted gene co-expression network analysis(WGCNA),the AP2/ERF transcription factor Rice Starch Regulator1 SiRSR1(Seita.3G044600)was identified as a key regulator of the salt stress response.To confirm its function,we generated OsRSR1-knockout lines using CRISPR/Cas9 genome editing in rice and its upstream repressor SimiR172a-overexpressing(172a-OE)transgenic plants in foxtail millet,which both showed increased salt tolerance.Overall,this study not only provides new insights into the convergent regulation of the salt stress responses of foxtail millet and rice,but it also sheds light on the divergent signaling networks between them in response to salt stress. 展开更多
关键词 C_(3) C_(4) PHYTOHORMONE PHOTOSYNTHESIS transcriptional regulatory network RSR1
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Discussion on the Key Role and Practice of Operating Room Nurses in Infection Control
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作者 meng wu 《Journal of Clinical and Nursing Research》 2025年第8期160-167,共8页
This paper focuses on the key role and practical paths of operating room nurses in infection control,and studies the difficult problems in the prevention and control of surgical site infections.Analyze the infection r... This paper focuses on the key role and practical paths of operating room nurses in infection control,and studies the difficult problems in the prevention and control of surgical site infections.Analyze the infection risk links in the operating room and the core responsibilities of nurses,reveal the multiple role mechanisms of nurses in infection control,design practical strategies from dimensions such as process optimization,skill improvement,and monitoring feedback,and construct an infection control system involving all staff.Research shows that operating room nurses can effectively reduce the risk of infection,improve medical quality,provide core guarantees for patient safety,and promote the transformation of operating room infection control towards refined management through practical actions such as standardized operation execution,real-time risk monitoring,and team collaboration supervision. 展开更多
关键词 Operating room nurse Infection control Surgical safety Nursing practice Risk prevention and control
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Potential inhibition of humic acid against soil-borne pathogenic fungi:A review
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作者 Shiping WEI Zhenqiang XIE +8 位作者 Yanling ZHAO Quanzhi WANG Yuanhua WANG Jiayin ZHAO Kaijun YIN Zhihao ZHU Yang LIU meng wu Zhongpei LI 《Pedosphere》 2025年第1期33-41,共9页
Green prevention and control management of soil-borne fungal diseases is a hot topic in agriculture,ecology,and the environment.It is an important way to effectively prevent and control soil-borne fungal diseases,solv... Green prevention and control management of soil-borne fungal diseases is a hot topic in agriculture,ecology,and the environment.It is an important way to effectively prevent and control soil-borne fungal diseases,solve soil degradation caused by continuous cropping obstacles,and fulfill the sustainable development of agriculture through revealing the mechanisms of functional substances to develop highly effective soil amendments.Humic acid shows an inhibitory effect on soil-borne pathogenic fungi,such as Fusarium oxysporum,Choanephora cucurbitarum,and Rhizoctonia solani,with the inhibition rate exceeding 80%.The molecular and elemental composition and contents of–COOH,phenolic C,methoxy group C,carboxyl C,aromatic C–O,anomeric C,and other functional groups of humic acid have been inferred to be responsible for its inhibitory effects on pathogenic fungi in previous research.The inhibitory mechanisms mainly include cell physiological morphology,biochemical process reactions,and molecular signal transduction.This review systematically summarizes the chemical structure,fungistatic effects,variable characteristics,and inhibitory mechanisms of humic acid,aiming to provide a theoretical basis for the development of green and efficient prevention and control technologies for soil-borne fungal diseases. 展开更多
关键词 active composition chemical structure inhibitory activity molecular mechanism soil-borne fungal diseases
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露天建材矿山高陡边坡生态修复技术研究
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作者 孟武 李天庆 +2 位作者 黄明 王太强 朱佳龙 《中国科技纵横》 2025年第20期27-30,共4页
针对露天建材矿山高陡边坡生态修复技术难题,本文从露天开采建材矿山高陡边坡的定义入手,阐述常见高陡边坡生态修复技术。通过对常见10多种生态修复技术方法的系统总结,优选9种适合高陡边坡生态修复的技术方法。此外,以武山县瓦碴沟石... 针对露天建材矿山高陡边坡生态修复技术难题,本文从露天开采建材矿山高陡边坡的定义入手,阐述常见高陡边坡生态修复技术。通过对常见10多种生态修复技术方法的系统总结,优选9种适合高陡边坡生态修复的技术方法。此外,以武山县瓦碴沟石灰岩矿山终了边坡生态修复工程为例,对比分析生态修复技术方法和坡面防护措施组合使用效果,总结生产建材矿山生态修复需要注意的问题,旨在为露天开采建材矿山高陡边坡生态修复工作提供指导和参考。 展开更多
关键词 露天开采 建材矿山 高陡边坡 生态修复技术 植物选择
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Sintering-and water-resistant perovskite quantum dots supported by inorganic materials for enhanced luminescence
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作者 meng wu Lingxiang Sun +7 位作者 Yanjie Zhang Feng Hong Xunzhu Jiang Xiuping wu Chunwen Ye Jingjie Yu Bing Li Botao Qiao 《Journal of Energy Chemistry》 2025年第9期508-516,I0014,共10页
Metal halide perovskite quantum dots(MHPQDs)have attracted intensive interest because of their unique optoelectronic properties.Their undesirable degradation upon exposure to humidity and/or heat,however,poses a dear ... Metal halide perovskite quantum dots(MHPQDs)have attracted intensive interest because of their unique optoelectronic properties.Their undesirable degradation upon exposure to humidity and/or heat,however,poses a dear challenge for the practical applications.Herein we report a facile strategy to develop sintering-resistant MHPQDs,e.g.CsPbBr_(3),by localizing them on the surface of inorganic support such as hydroxyapatite(HAP).The chemical interaction between CsPbBr_(3)quantum dots(QDs)and HAP support originates from the occupation of Br vacancies in CsPbBr_(3)by the-O-on the surface of HAP support,which not only stabilizes the small particle sizes(~2.2 nm)of CsPbBr_(3)QDs upon high-temperature(up to 400℃)calcination but also greatly enhances its photoluminescence emission intensity by about150 times.Interestingly,the supported CsPbBr_(3)QDs decorated by cetyltrimethylammonium bromide can further produce water-resistant CsPbBr_(3)@CsPb_(2)Br_(5)QDs.The obtained sintering-resistant hydroxyapatite-supported CsPbBr_(3)@CsPb_(2)Br_(5)QDs can be used to fabricate green light emitting diodes(LED)devices with high luminous intensity for medicolegal identification,flexible luminescence film for display,and potential fluorescent label for bioimaging/biosensing applications.This work demonstrates a novel strategy to design and develop robust all-inorganic QDs composites that may find wide applications in diverse environmental conditions,including high temperature and/or high humidity. 展开更多
关键词 Perovskite QDs-support interaction Water-resistant Perovskites Photoluminescence
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Improving the interpretation of undrained shear strength from piezocone penetration tests by integrating soil physical properties using a hybrid meta-heuristic algorithm
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作者 meng wu Zening Zhao Guojun Cai 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第5期3180-3197,共18页
Conventional empirical equations for estimating undrained shear strength(s_(u))from piezocone penetration test(CPTu)data,without incorporating soil physical properties,often lack the accuracy and robustness required f... Conventional empirical equations for estimating undrained shear strength(s_(u))from piezocone penetration test(CPTu)data,without incorporating soil physical properties,often lack the accuracy and robustness required for geotechnical site investigations.This study introduces a hybrid virus colony search(VCS)algorithm that integrates the standard VCS algorithm with a mutation-based search mechanism to develop high-performance XGBoost learning models to address this limitation.A dataset of 372 seismic CPTu and corresponding soil physical properties data from 26 geotechnical projects in Jiangs_(u)Province,China,was collected for model development.Comparative evaluations demonstrate that the proposed hybrid VCS-XGBoost model exhibits s_(u)perior performance compared to standard meta-heuristic algorithm-based XGBoost models.The res_(u)lts highlight that the consideration of soil physical properties significantly improves the predictive accuracy of s_(u),emphasizing the importance of considering additional soil information beyond CPTu data for accurate s_(u)estimation. 展开更多
关键词 Undrained shear strength Piezocone penetration test Extreme gradient boosting Meta-heuristic algorithm
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贴近摄影测量技术在矿山高陡边坡稳定性调查评价中的应用
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作者 孟武 王太强 +2 位作者 黄欣 李建全 吕周霁 《世界有色金属》 2025年第19期166-168,共3页
关子镇露天矿山边坡存在失稳风险,为确保人员安全,亟需对边坡进行稳定性调查评价。本文采用贴近摄影测量技术,通过航线规划、航测成图等步骤得到矿区内地形二维图片,在此基础上,利用SfM三维重构,将二维图片转变为三维地形图并继续使用... 关子镇露天矿山边坡存在失稳风险,为确保人员安全,亟需对边坡进行稳定性调查评价。本文采用贴近摄影测量技术,通过航线规划、航测成图等步骤得到矿区内地形二维图片,在此基础上,利用SfM三维重构,将二维图片转变为三维地形图并继续使用结构面智能识别法,确定稳定性较差的边坡,最终应用赤平极射投影法进行稳定性分析。事实证明,该边坡存在失稳风险,通过提前预防可以有效避免次生灾害的发生。 展开更多
关键词 贴近摄影测量技术 边坡稳定性评价 赤平极射投影 无人机
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Rational design of MXene@VS_(4) heterostructures via interfacial coupling for advanced magnesium-ion batteries
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作者 Xinyu Zhang Wenxin Li +3 位作者 Meihan Sun meng wu Fanfan Liu Dan Zhou 《Journal of Energy Chemistry》 2025年第10期566-575,共10页
Rechargeable magnesium batteries(RMBs)have garnered significant attention in energy storage applications due to their high capacity,low cost,and high safety.However,the strong polarization effect and slow kinetic de-i... Rechargeable magnesium batteries(RMBs)have garnered significant attention in energy storage applications due to their high capacity,low cost,and high safety.However,the strong polarization effect and slow kinetic de-intercalation of Mg^(2+)in the cathode limit their commercial application.This study presents a novel interface-coupled V_(2)CT_(x)@VS_(4)heterostructure through a one-step hydrothermal process.In this architecture,V_(2)CT_(x)and VS_(4)can mutually support their structural framework,which effectively prevents the structural collapse of V_(2)CT_(x)MXene and the aggregation of VS_(4).Crucially,interfacial coupling between V_(2)CT_(x)and VS_(4)induces strong V-S bonds,substantially enhancing structural stability.Benefiting from these advantages,the heterostructure exhibits high specific capacity(226 mAh g^(-1)at 100 mA g^(-1))and excellent long-cycle stability(89% capacity retention after 1000 cycles at 500 mA g^(-1)).Furthermore,the Mg^(2+)storage mechanism in the V_(2)CT_(x)@VS_(4)composite was elucidated through a series of ex-situ characterizations.This work provides a feasible strategy for designing V_(2)CT_(x)MXene-based cathodes with high capacity and extended cyclability for RMBs. 展开更多
关键词 MXene HETEROSTRUCTURE Reaction mechanism Interfacial coupling
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细胞自噬基因Atg6在涡虫中枢神经系统再生中的功能研究 被引量:1
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作者 马克学 李睿 +4 位作者 郭芳莹 宋鸽鸽 吴萌 陈广文 刘德增 《遗传》 CAS CSCD 北大核心 2021年第8期792-801,I0001,共11页
细胞自噬基因Atg6在细胞自噬过程中发挥重要作用,其功能缺陷影响神经发生。涡虫是研究中枢神经系统(central nervous system,CNS)再生的良好模型,其头部切除后1周就能再生出一个新的头部。因此,研究Atg6基因在涡虫CNS再生中的作用对探... 细胞自噬基因Atg6在细胞自噬过程中发挥重要作用,其功能缺陷影响神经发生。涡虫是研究中枢神经系统(central nervous system,CNS)再生的良好模型,其头部切除后1周就能再生出一个新的头部。因此,研究Atg6基因在涡虫CNS再生中的作用对探究自噬调控神经发生具有重要意义。本研究首次报道了日本三角涡虫(Dugesia japonica)Atg6基因(DjAtg6)的分子特征,并利用RNAi技术研究了其在涡虫CNS再生中作用。结果显示:DjAtg6 cDNA全长1366 bp,编码423个氨基酸。DjATG6含有ATG6/Beclin 1蛋白家族的Coil-Coil结构域和β折叠α螺旋自噬功能结构域。涡虫沿咽前咽后切割后,DjAtg6表达量显著增加,其转录本主要在新再生的脑神经节表达。RNAi-DjAtg6引起涡虫头部再生迟缓、脑神经结构偏小,并下调神经相关基因的表达。此外,本研究还发现,RNAi-DjAtg6不影响涡虫干细胞的增殖,但下调细胞迁移相关基因mmp1和mmp2的表达,且干扰mmp1和mmp2的表达影响涡虫头再生。因此,本研究结果表明,DjAtg6在涡虫CNS再生的组织重构中发挥重要作用,干扰DjAtg6影响涡虫CNS再生可能与细胞迁移有关,其详细的分子机制尚需进行深入研究。 展开更多
关键词 涡虫 细胞自噬 Atg6 再生 RNA干扰
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科教融合背景下的“生物化学”教学改革与实践 被引量:3
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作者 丁烽 张兴丽 +1 位作者 孟武 李丕武 《教育教学论坛》 2023年第44期70-73,共4页
以齐鲁工业大学与山东省科学院深度融合2.0为背景,科教产多方协同育人,构建了一种适用于生物专业应用创新型人才的生物化学教学模式并实践。确立“以学生为中心”的人才培养理念,以培养学生创新能力、实践能力和分析问题、解决问题的思... 以齐鲁工业大学与山东省科学院深度融合2.0为背景,科教产多方协同育人,构建了一种适用于生物专业应用创新型人才的生物化学教学模式并实践。确立“以学生为中心”的人才培养理念,以培养学生创新能力、实践能力和分析问题、解决问题的思维能力为核心,修订适合科教产融合背景的教学方案,构建一种体现科教产深度融合的“生物化学”课程教学模式,实现科研优势、资源优势向人才培养优势的转换,从而满足新工科对创新型应用人才培养的需求。 展开更多
关键词 科教融合 生物化学 创新型应用人才培养 教学改革与实践
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科教融合培养生物工程专业学生创新创业能力 被引量:1
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作者 孟武 丁烽 +1 位作者 黄晓文 刘洪玲 《教育教学论坛》 2024年第41期168-172,共5页
齐鲁工业大学(山东省科学院)生物工程学部的成立是科教融合的结果。针对现有的生物工程专业学生教育过程存在重理论学习、轻实践能力培养,培养出的很大一部分学生走向企业时出现不接地气的现象,基于科教融合“双轮驱动”优势,突破高校... 齐鲁工业大学(山东省科学院)生物工程学部的成立是科教融合的结果。针对现有的生物工程专业学生教育过程存在重理论学习、轻实践能力培养,培养出的很大一部分学生走向企业时出现不接地气的现象,基于科教融合“双轮驱动”优势,突破高校传统办学模式,深度挖掘教学资源要素、科研机构资源要素和产业创新要素,强化教科产协同创新,促进资源共享,有效整合学校办学过程中的多样化资源和要素,实现教学理论与科技创新及产业创新相结合,突破创新创业人才培养的瓶颈,构建面向生物工程产业发展需求的人才培养结构和供需机制,全面提升创新应用型人才培养质量。 展开更多
关键词 教科产融合 生物工程专业 人才培养 创新创业能力
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Lax Facial Skin Treated with High-Intensity Focused Ultrasound Devices: Efficacy and Safety Evaluations
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作者 meng wu Huan Chen 《Journal of Cosmetics, Dermatological Sciences and Applications》 2023年第3期1455-1472,共9页
Background: High-intensity focused ultrasound (HIFU) has been introduced to improve skin laxity in recent years. However, very few studies have evaluated the safety and effectiveness of HIFU in Chinese populations. Me... Background: High-intensity focused ultrasound (HIFU) has been introduced to improve skin laxity in recent years. However, very few studies have evaluated the safety and effectiveness of HIFU in Chinese populations. Methods: In the study, 30 Chinese participants underwent HIFU (Bolida, Inc., Changsha, China) rejuvenation between February 1, 2022, and September 30, 2022. There were three different focal depths used depending on the area where shots were captured (4.5 mm, 4 MHz;3 mm, 7 MHz;1.5 mm, 7 MHz). After 3 months and 6 months of treatment, efficacy and safety were assessed by quantitative analysis. Results: Patients were satisfied with the clinical effects of HIFU rejuvenation after one session. In terms of effectiveness, HIFU was most successful in areas around the jawline, cheek, and perioral. In four cases, erythema was observed, in two cases, swollen gums were seen, but all of these effects were transient and mild. Conclusion: Bolida system can be safe and effective for facial tightening, additionally, they are most effective for jawline, cheek, and perioral improvements. In clinical practice, the Bolida system can be recommended as a reliable treatment option. . 展开更多
关键词 High-Intensity Focused Ultrasound Bolida System TREATMENT EFFICACY SAFETY
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高效液相色谱法检测枯草芽孢杆菌发酵液中乙偶姻和2,3-丁二醇含量 被引量:3
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作者 毛林静 孟武 +3 位作者 王莹 王婧臻 王慧 楚杰 《食品工业科技》 CAS 北大核心 2020年第17期253-257,共5页
枯草芽孢杆菌可发酵代谢产生乙偶姻和2,3-丁二醇(两者可逆转化),本研究建立了用高效液相色谱法同时检测发酵液中乙偶姻和2,3-丁二醇的方法。对比了不同色谱柱和流动相配比对乙偶姻和2,3-丁二醇含量的测定结果,进一步检测枯草芽孢杆菌发... 枯草芽孢杆菌可发酵代谢产生乙偶姻和2,3-丁二醇(两者可逆转化),本研究建立了用高效液相色谱法同时检测发酵液中乙偶姻和2,3-丁二醇的方法。对比了不同色谱柱和流动相配比对乙偶姻和2,3-丁二醇含量的测定结果,进一步检测枯草芽孢杆菌发酵液中乙偶姻和2,3-丁二醇含量。结果表明:采用Bio-Rad Aminex HPX-87H Column分析;流动相:0.005 mol/L的硫酸溶液;流速:0.5 mL/min;柱温:28℃;示差检测器,在30 min内完成样品检测,乙偶姻和2,3-丁二醇标准曲线相关系数分别为0.9997和0.9998,精密度标准偏差分别为1.76%和1.74%,稳定性标准偏差分别为1.2%和1.6%,回收率为98.93%~102.10%,相对标准偏差≤2%。此方法简单准确,检测目标物浓度为50 g/L,检测结果重现性好,发酵液中乙偶姻和2,3-丁二醇含量呈负相关,乙偶姻的含量随着2,3-丁二醇的增加而降低,该方法适合枯草芽孢杆菌发酵液中乙偶姻和2,3-丁二醇的发酵检测。 展开更多
关键词 枯草芽孢杆菌 乙偶姻 2 3-丁二醇 高效液相色谱法
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白酒酿造过程中塑化剂风险控制措施研究 被引量:4
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作者 吕晓凤 孟武 +7 位作者 吕志远 刘玉涛 张梦梦 卢春玲 李强 高红波 邱振清 王瑞明 《酿酒科技》 2024年第1期72-77,共6页
白酒酿造是多种微生物利用粮食等原料进行发酵的过程,此过程不会产生塑化剂,但白酒生产环节如原辅料的输送、输酒管道、储存容器、与酒直接接触包装罐装制品、成品包装用塑料袋或塑料瓶(桶)等,均存在塑化剂迁移的潜在风险。为追溯白酒... 白酒酿造是多种微生物利用粮食等原料进行发酵的过程,此过程不会产生塑化剂,但白酒生产环节如原辅料的输送、输酒管道、储存容器、与酒直接接触包装罐装制品、成品包装用塑料袋或塑料瓶(桶)等,均存在塑化剂迁移的潜在风险。为追溯白酒生产过程中塑化剂的来源,并规避这些风险,从白酒原辅料、白酒生产、储存、包装等全产业链进行调查研究,对存在塑化剂迁移的风险点,进行模拟实验,采用GC-MS方法,对模拟实验样品进行检测并深入分析,找出影响白酒中塑化剂含量的主要因素,对其中的风险点进行风险等级划分并给出具体控制措施(在调酒池刚做好防腐涂层时先用食用酒精浸泡数次,经检测合格后再使用;建议使用不锈钢管替换次灌装线的塑料软管),实验有效地控制和降低了白酒中塑化剂含量,从而保证了白酒的产品品质。本研究结果对白酒企业在酿造产业链预防和控制塑化剂污染的风险具有重要的的指导意义,可促进白酒产业的良性可持续发展。 展开更多
关键词 白酒生产 塑化剂 来源 风险等级 白酒品质
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