Photo-Cross-Linkable hydrogel has attracted immense interest in the regeneration of bone repair and regeneration strategies due to its superior biocompatibility and tunable mechanical properties.Recently,Nb was report...Photo-Cross-Linkable hydrogel has attracted immense interest in the regeneration of bone repair and regeneration strategies due to its superior biocompatibility and tunable mechanical properties.Recently,Nb was reported to strongly promote the bone regeneration process via an accelerated osteoblast-modulated alkaline phosphatase activity mechanism.In particular,Nb2C MXenes have drawn widespread attention due to their excellent biocompatibility and ability to induce bone formation.However,the easy agglomeration of Nb2C nanosheets and subsequent low cell endocytosis efficacy greatly suppressed the osteogenesis effect.In this study,a subtractive nanopore-engineered Nb2C MXene was prepared through a microwave combustion method,gelatin methacrylate was used as the carrier hydrogel,and the photo-triggered Porous-Nb2C@GelMA hydrogel was fabricated by a photo-triggered process.The pore-forming strategy not only successfully improved the distribution of Nb2C and formed more homogenous Porous-Nb2C@GelMA hydrogels but also guided bone marrow mesenchymal stem cells(BMSCs)toward osteoblast differentiation.Porous Nb2C provided convenient cellular grasping and endocytosis for BMSCs,which further created a favorable environment for differentiation and osteogenesis.This,in turn,leads to an increase in the expression of osteogenic markers,such as ALP and ARS,as well as osteogenic factors,such as BMP-2,COL-1,OCN,and OPN.Consequently,enhancing the regenerative microenvironment by incorporating porous Nb2C composite hydrogels shows promise for application in bone regeneration.展开更多
目的探讨circKIF4A对卵巢癌SKOV3细胞增殖、凋亡的影响及其对miR-384的调控作用机制。方法采用q RT-PCR法检测卵巢癌组织与癌旁组织中circKIF4A、miR-384的表达量;分别将si-NC、sicircKIF4A、miR-NC、miR-384 mimics、si-circKIF4A与ant...目的探讨circKIF4A对卵巢癌SKOV3细胞增殖、凋亡的影响及其对miR-384的调控作用机制。方法采用q RT-PCR法检测卵巢癌组织与癌旁组织中circKIF4A、miR-384的表达量;分别将si-NC、sicircKIF4A、miR-NC、miR-384 mimics、si-circKIF4A与anti-miR-NC、si-circKIF4A与anti-miR-384转染入SKOV3细胞;采用q RT-PCR法检测SKOV3细胞中circKIF4A、miR-384的表达量;采用MTT实验与流式细胞术分别检测细胞增殖及凋亡能力;双荧光素酶报告实验检测circKIF4A与miR-384的靶向关系。结果与癌旁组织比较,卵巢癌组织中circKIF4A的表达水平升高[(1.00±0.06),(4.28±0.32)](t=62.915, P <0.05),miR-384的表达水平降低[(1.00±0.05),(0.43±0.03)](t=61.047, P <0.05);转染si-circKIF4A后SKOV3细胞OD值降低[(0.75±0.05),(0.41±0.03)](t=17.493, P <0.05),凋亡率升高[(6.36±0.53)%,(23.19±2.21)%](t=22.216,P <0.05);转染miR-384 mimics后SKOV3细胞OD值降低[(0.73±0.05),(0.47±0.04)](t=12.182, P <0.05),凋亡率升高[(7.53±0.41)%,(19.11±1.06)%](t=30.567, P <0.05);双荧光素酶报告实验证实circKIF4A能够吸附miR-384,并可充当miR-384的海绵分子;共转染si-circKIF4A与anti-miR-384后SKOV3细胞OD值升高[(0.40±0.04),(0.65±0.03)](t=15.000, P <0.05),凋亡率降低[(25.20±2.21)%,(10.37±0.86)%](t=18.761, P <0.05)。结论抑制circKIF4A表达可负向调控miR-384的表达从而抑制卵巢癌细胞增殖及诱导细胞凋亡。展开更多
To investigate the role of oxygen defects on the photocatalytic activity of TiO2,the TiO2 nanocrystals with/without oxygen defects are successfully synthesized by the hydrothermal and sol‐gel methods,respectively.The...To investigate the role of oxygen defects on the photocatalytic activity of TiO2,the TiO2 nanocrystals with/without oxygen defects are successfully synthesized by the hydrothermal and sol‐gel methods,respectively.The as‐prepared TiO2 nanocrystals with defects are light blue and the absorption edge of light is towards the visible light region(~420 nm).Raman and X‐ray photoelectron spectroscopy(XPS)measurements all confirm that the concentration of oxygen vacancies in the TiO2 synthesized by the sol‐gel method is less than that synthesized through the hydrothermal route.The introduction of oxygen defects contributes to a new state in the band gap that narrows the band gap,which is the reason for the extension of light absorption into the visible light region.The photocurrent results confirm that this band‐gap narrowing enhances the photocurrent response under simulated solar light irradiation.The TiO2 with oxygen defects shows a higher photocatalytic activity for decomposition of a methylene blue solution compared with that of the perfect TiO2 sample.The photocatalytic mechanism is discussed based on the density functional theory calculations and photoluminescence spectroscopy measurements.展开更多
基金supported by the Natural Science Foundation of China(82202751,Derong Xu,82372478,Chuanli Zhou,62375249,U1803128,Meng Qiu)the Taishan Scholar Project(tsqn202211362,Chuanli Zhou,tsqn201909054,Meng Qiu)+1 种基金the Natural Science Foundation of Shandong Province(ZR2021MH020,Chuanli Zhou,ZR2022JQ22,Meng Qiu)the Fundamental Research Funds for the Central Universities(202341006,Meng Qiu)。
文摘Photo-Cross-Linkable hydrogel has attracted immense interest in the regeneration of bone repair and regeneration strategies due to its superior biocompatibility and tunable mechanical properties.Recently,Nb was reported to strongly promote the bone regeneration process via an accelerated osteoblast-modulated alkaline phosphatase activity mechanism.In particular,Nb2C MXenes have drawn widespread attention due to their excellent biocompatibility and ability to induce bone formation.However,the easy agglomeration of Nb2C nanosheets and subsequent low cell endocytosis efficacy greatly suppressed the osteogenesis effect.In this study,a subtractive nanopore-engineered Nb2C MXene was prepared through a microwave combustion method,gelatin methacrylate was used as the carrier hydrogel,and the photo-triggered Porous-Nb2C@GelMA hydrogel was fabricated by a photo-triggered process.The pore-forming strategy not only successfully improved the distribution of Nb2C and formed more homogenous Porous-Nb2C@GelMA hydrogels but also guided bone marrow mesenchymal stem cells(BMSCs)toward osteoblast differentiation.Porous Nb2C provided convenient cellular grasping and endocytosis for BMSCs,which further created a favorable environment for differentiation and osteogenesis.This,in turn,leads to an increase in the expression of osteogenic markers,such as ALP and ARS,as well as osteogenic factors,such as BMP-2,COL-1,OCN,and OPN.Consequently,enhancing the regenerative microenvironment by incorporating porous Nb2C composite hydrogels shows promise for application in bone regeneration.
文摘目的探讨circKIF4A对卵巢癌SKOV3细胞增殖、凋亡的影响及其对miR-384的调控作用机制。方法采用q RT-PCR法检测卵巢癌组织与癌旁组织中circKIF4A、miR-384的表达量;分别将si-NC、sicircKIF4A、miR-NC、miR-384 mimics、si-circKIF4A与anti-miR-NC、si-circKIF4A与anti-miR-384转染入SKOV3细胞;采用q RT-PCR法检测SKOV3细胞中circKIF4A、miR-384的表达量;采用MTT实验与流式细胞术分别检测细胞增殖及凋亡能力;双荧光素酶报告实验检测circKIF4A与miR-384的靶向关系。结果与癌旁组织比较,卵巢癌组织中circKIF4A的表达水平升高[(1.00±0.06),(4.28±0.32)](t=62.915, P <0.05),miR-384的表达水平降低[(1.00±0.05),(0.43±0.03)](t=61.047, P <0.05);转染si-circKIF4A后SKOV3细胞OD值降低[(0.75±0.05),(0.41±0.03)](t=17.493, P <0.05),凋亡率升高[(6.36±0.53)%,(23.19±2.21)%](t=22.216,P <0.05);转染miR-384 mimics后SKOV3细胞OD值降低[(0.73±0.05),(0.47±0.04)](t=12.182, P <0.05),凋亡率升高[(7.53±0.41)%,(19.11±1.06)%](t=30.567, P <0.05);双荧光素酶报告实验证实circKIF4A能够吸附miR-384,并可充当miR-384的海绵分子;共转染si-circKIF4A与anti-miR-384后SKOV3细胞OD值升高[(0.40±0.04),(0.65±0.03)](t=15.000, P <0.05),凋亡率降低[(25.20±2.21)%,(10.37±0.86)%](t=18.761, P <0.05)。结论抑制circKIF4A表达可负向调控miR-384的表达从而抑制卵巢癌细胞增殖及诱导细胞凋亡。
基金supported by the National Natural Science Foundation of China(51602207)the Doctoral Scientific Research Foundation of Shenyang Medical College(20174043)+1 种基金the Scientific Research Project for University Students of Shenyang Medical College(20160809)the Doctoral Scientific Research Foundation of Liaoning Province(201601149,20170520011)~~
文摘To investigate the role of oxygen defects on the photocatalytic activity of TiO2,the TiO2 nanocrystals with/without oxygen defects are successfully synthesized by the hydrothermal and sol‐gel methods,respectively.The as‐prepared TiO2 nanocrystals with defects are light blue and the absorption edge of light is towards the visible light region(~420 nm).Raman and X‐ray photoelectron spectroscopy(XPS)measurements all confirm that the concentration of oxygen vacancies in the TiO2 synthesized by the sol‐gel method is less than that synthesized through the hydrothermal route.The introduction of oxygen defects contributes to a new state in the band gap that narrows the band gap,which is the reason for the extension of light absorption into the visible light region.The photocurrent results confirm that this band‐gap narrowing enhances the photocurrent response under simulated solar light irradiation.The TiO2 with oxygen defects shows a higher photocatalytic activity for decomposition of a methylene blue solution compared with that of the perfect TiO2 sample.The photocatalytic mechanism is discussed based on the density functional theory calculations and photoluminescence spectroscopy measurements.
