[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant ge...[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant genes of 24 strains of pathogenic K.peneumoniae from racoon dog to fluoroquinolones were detected by K-B drug susceptibility paper and PCR.[Result]Multiple drug-resistant strains were dominant among 24 strains of pathogenic K.peneumoniae,and the drug resistance to levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin was 70.8%-79.2%;the strains resistant to 4,3 and 2 antibiotics were 58.3%,29.2%and 8.3%,respectively.The detection rates of drug-resistant genes TEM,gyrB,parC,parE,gyrA and SHV were 75%-100%,and the number of strains carrying 9,8,7,6,4 and 3 drug-resistant genes was 4.2%-41.7%.Comparison of drug-resistant genes and drug resistance showed that the coincidence rates of levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin were 78.9%-100%.[Conclusion]There were certain correlation between drug resistance and resistant genotypes of 24 strains of pathogenic Klebsiella peneumo-niae to fluoroquinolones.展开更多
植物小分子热激蛋白(small heat shock proteins, s HSPs)是一种多样、古老的蛋白家族,分子量大小普遍在12~42 k D之间,包含典型的C端、N端和α晶状体蛋白域。s HSPs作为一种重要的分子伴侣在植物生长发育和响应逆境胁迫中起着重要的作...植物小分子热激蛋白(small heat shock proteins, s HSPs)是一种多样、古老的蛋白家族,分子量大小普遍在12~42 k D之间,包含典型的C端、N端和α晶状体蛋白域。s HSPs作为一种重要的分子伴侣在植物生长发育和响应逆境胁迫中起着重要的作用。本研究通过构建成熟期番茄果实cDNA文库,筛选与SlHSP17.7互作的蛋白,进而探索SlHSP17.7在果实发育进程中的分子机制。以Micro-Tom番茄绿熟期、转色期和完熟期果实cDNA为模板,构建番茄果实c DNA文库。构建pGBKT7-SlHSP17.7诱饵载体筛选与其互作的蛋白,利用酵母双杂交实验验证互作关系。经检测番茄果实cDNA文库库容是2.2×10~6CFU,工作液细胞密度是3.6×10~7cell/mL,均一化程度>106,结果符合质控要求。将pGBKT7-SlHSP17.7诱饵载体转化Y2HGold酵母菌株检测发现,其无毒性和自激活现象,经筛选c DNA文库、测序及序列比对分析得到钙阳离子交换体SlCCX1-like蛋白,利用酵母双杂交实验验证发现二者存在互作关系。经PCR鉴定后,所建文库质量良好,文库的库容和文库滴度均符合建库要求,用pGBKT7-SlHSP17.7诱饵载体筛选得到互作蛋白SlCCX1-like,为进一步研究在果实发育进程SlHSP17.7的生物学功能提供了保障。展开更多
基金Supported by Project of Hebei Department of Science and Technology(18246629G)Project of Shijiazhuang Science and Technology Bureau(171500953A)Project of Hebei Department of Education(ZD2017234)
文摘[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant genes of 24 strains of pathogenic K.peneumoniae from racoon dog to fluoroquinolones were detected by K-B drug susceptibility paper and PCR.[Result]Multiple drug-resistant strains were dominant among 24 strains of pathogenic K.peneumoniae,and the drug resistance to levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin was 70.8%-79.2%;the strains resistant to 4,3 and 2 antibiotics were 58.3%,29.2%and 8.3%,respectively.The detection rates of drug-resistant genes TEM,gyrB,parC,parE,gyrA and SHV were 75%-100%,and the number of strains carrying 9,8,7,6,4 and 3 drug-resistant genes was 4.2%-41.7%.Comparison of drug-resistant genes and drug resistance showed that the coincidence rates of levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin were 78.9%-100%.[Conclusion]There were certain correlation between drug resistance and resistant genotypes of 24 strains of pathogenic Klebsiella peneumo-niae to fluoroquinolones.
