INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depres...INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depression leads to social and occupational impairment,diminished quality of life and an elevated risk of death by suicide.展开更多
目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和...目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。展开更多
Oxygen vacancy engineering is a valid strategy to boost the oxygen reduction reaction(ORR) performance of nanostructured electrocatalysts.Current methods for generating surface oxygen vacancies(Vos) in nanostructured ...Oxygen vacancy engineering is a valid strategy to boost the oxygen reduction reaction(ORR) performance of nanostructured electrocatalysts.Current methods for generating surface oxygen vacancies(Vos) in nanostructured MnO_(2) is mostly lab-scale,which cannot meet the requirement of large-scale production.Herein,we employed a mechanochemical method of ball milling to introduce surface Vos into the β-MnO_(2) nanoparticles.The ball milling process generated abundant surface Vos,which significantly facilitated the adsorption and activation of O_(2).Consequently,the ORR performance of ball-milled β-MnO_(2) was markedly boosted by varying the ball milling time.As an air cathode catalyst for zinc-air battery(ZAB),the β-MnO_(2) ball-milled for 4 h displayed a high specific capacity of 804 mA·h·g^(-1) and excellent cycling over 500 h at 5 mA·cm^(-2),which were superior than those of pristine β-MnO_(2)-based ZAB.Our work offers a feasible strategy to enhance electrocatalytic ORR performance of MnO_(2),which shows significant potential for large-scale production of efficient ORR electrocatalysts.展开更多
【目的】应用多种分子标记,评估其在鉴定广州市花都区花山镇野鼠粪便中的广州管圆线虫的有效性,并分析野鼠的感染状况。【方法】捕获广州花都区花山镇128只野鼠,采集野鼠粪便,采用FastDNA SPIN Kit for Soil试剂盒提取粪便总DNA,通过PC...【目的】应用多种分子标记,评估其在鉴定广州市花都区花山镇野鼠粪便中的广州管圆线虫的有效性,并分析野鼠的感染状况。【方法】捕获广州花都区花山镇128只野鼠,采集野鼠粪便,采用FastDNA SPIN Kit for Soil试剂盒提取粪便总DNA,通过PCR扩增获得cytb、cox I和ITS2片段,PCR产物经测序鉴定,并对测序产物进行系统发育分析。【结果】通过PCR扩增和测序鉴定,仅有5份样本扩增出的cytb序列没有套峰,可以准确鉴定为广州管圆线虫。结果表明该地区褐家鼠的广州管圆线虫感染率为7.58%(5/66),而黄胸鼠、黄毛鼠、臭鼩鼱及板齿鼠中未检测到cytb序列,表明褐家鼠是广州管圆线虫的适宜宿主,且cytb序列适用于从野鼠粪便中鉴定广州管圆线虫的感染情况。【结论】Cytb序列是鉴定野鼠粪便中广州管圆线虫的良好分子标记,可以用于长期监测野鼠中的广州管圆线虫流行情况和危害潜力。展开更多
Bacterial infection is a very troublesome issue in wound treatment, which stimulates exudate formation and severely delays the healing process. Herein, a thermogelling dressing system composed of two triblock copolyme...Bacterial infection is a very troublesome issue in wound treatment, which stimulates exudate formation and severely delays the healing process. Herein, a thermogelling dressing system composed of two triblock copolymers of poly(D,L-lactic acid-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(D,L-lactic acid-co-glycolic acid)(PLGA-PEG-PLGA) with different block lengths was developed to deliver teicoplanin(TPN), a glycopeptide antibiotic, for cutaneous wound repair. The TPN-loaded thermogel was a free-flowing sol at room temperature and formed a semi-solid gel at physiological temperature. In vitro studies demonstrated that the TPN-loaded thermogel system exhibited desired tissue adhesiveness and realized the sustained release of TPN in a fast-followed-slow manner for over three weeks. Furthermore, a full-thickness excision wound model in Sprague-Dawley(SD) rats was constructed to assess the efficacy of TPNloaded thermogel formulation. Gross and histopathologic observations implied that treatment with the thermogel formulation reduced inflammation response, promoted disposition of collagen, enhanced angiogenesis, and accelerated wound closure and maturity of SD rats.The combination of the bioactivity of TPN and the acidic nature of the thermogel matrix was responsible for such an enhanced wound healing process. Consequently, the TPN-loaded PLGA-PEG-PLGA thermogel is a good candidate of wound dressing for full-thickness excision wound healing.展开更多
基金funded by the Construction Project of the"Flagship"Department of Chinese and Western Medicine Coordination(LiuL/2024-221)the 2024 Medical Service and Security Capacity Improvement Project(National Clinical Key Specialty Construction)(LiuL/Huwei Medical/2024-65)+5 种基金the Shanghai Traditional Chinese Medicine Standardization Project(LiuL/No.2023JSP03)the Shanghai Key Discipline Construction Project of Traditional Chinese Medicine(Clinical)(LiuL/2024-No.3)the Shanghai Technical Standardization Management and Promotion Project(LiuL/No.SHDC22023212)the Shanghai Municipal Health Commission Traditional Chinese Medicine Research Project(2022)(LiuL/No.2022Cx004)Clinical research project of Shanghai Health Commission-Youth Project(LW/No.20214Y0056)Shanghai Institute of Traditional Chinese Medicine for Mental Health(LW/No.SZB2023201).
文摘INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depression leads to social and occupational impairment,diminished quality of life and an elevated risk of death by suicide.
文摘目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。
基金financially supported by the Science and Technology Program of Guangzhou (202201010373)。
文摘Oxygen vacancy engineering is a valid strategy to boost the oxygen reduction reaction(ORR) performance of nanostructured electrocatalysts.Current methods for generating surface oxygen vacancies(Vos) in nanostructured MnO_(2) is mostly lab-scale,which cannot meet the requirement of large-scale production.Herein,we employed a mechanochemical method of ball milling to introduce surface Vos into the β-MnO_(2) nanoparticles.The ball milling process generated abundant surface Vos,which significantly facilitated the adsorption and activation of O_(2).Consequently,the ORR performance of ball-milled β-MnO_(2) was markedly boosted by varying the ball milling time.As an air cathode catalyst for zinc-air battery(ZAB),the β-MnO_(2) ball-milled for 4 h displayed a high specific capacity of 804 mA·h·g^(-1) and excellent cycling over 500 h at 5 mA·cm^(-2),which were superior than those of pristine β-MnO_(2)-based ZAB.Our work offers a feasible strategy to enhance electrocatalytic ORR performance of MnO_(2),which shows significant potential for large-scale production of efficient ORR electrocatalysts.
基金financially supported by the National Natural Science Foundation of China (Nos. 51773043, 81772363, and 21474019)National Key R&D Program of China (No. 2016YFC1100300)China Postdoctoral Science Foundation (No. 2018M632020)
文摘Bacterial infection is a very troublesome issue in wound treatment, which stimulates exudate formation and severely delays the healing process. Herein, a thermogelling dressing system composed of two triblock copolymers of poly(D,L-lactic acid-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(D,L-lactic acid-co-glycolic acid)(PLGA-PEG-PLGA) with different block lengths was developed to deliver teicoplanin(TPN), a glycopeptide antibiotic, for cutaneous wound repair. The TPN-loaded thermogel was a free-flowing sol at room temperature and formed a semi-solid gel at physiological temperature. In vitro studies demonstrated that the TPN-loaded thermogel system exhibited desired tissue adhesiveness and realized the sustained release of TPN in a fast-followed-slow manner for over three weeks. Furthermore, a full-thickness excision wound model in Sprague-Dawley(SD) rats was constructed to assess the efficacy of TPNloaded thermogel formulation. Gross and histopathologic observations implied that treatment with the thermogel formulation reduced inflammation response, promoted disposition of collagen, enhanced angiogenesis, and accelerated wound closure and maturity of SD rats.The combination of the bioactivity of TPN and the acidic nature of the thermogel matrix was responsible for such an enhanced wound healing process. Consequently, the TPN-loaded PLGA-PEG-PLGA thermogel is a good candidate of wound dressing for full-thickness excision wound healing.
