Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potentia...Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potential link with enhanced exercise performance,reducing the risk of various chronic diseases.In this study,we will observe the impact of SJZT on the exercise performance of mice and explore the underlying mechanism.Methods:In this study,we used the steam distillation method to obtain the extract of SJZT.Mice were given pure water,low and high doses of SJZT respectively for 4 weeks,followed by the assessment of exercise performance(Forelimb Grip Strength Test,Exhausted Swimming Training,Exhausted Treadmill Running Test,and 10-min Swimming Test).Subsequently,serum and urine biochemical indices,glycogen content,liver mRNA,and protein expression were analyzed after the mice completed 6 weeks of treadmill training.In the cellular experiments,we used the HepG2 cell line treated with SJZT post-gavage serum to mimic the in-vivo SJZT-administered mouse model,and used Western blot,qPCR,and other experimental techniques to thoroughly investigate the potential mechanism of SJZT to enhance exercise performance.Results:Compared with the Vehicle group,the SJZT groups showed increased lactate clearance and elevated liver glycogen levels.In terms of exercise performance,grip strength,exercise capacity,and lactate clearance were increased,and lactate production was decreased in the SJZT groups.In comparison to the Vehicle group,the SJZT groups exhibited decreased RNA and protein expression of p-p38 MAPK,increased RNA and protein expression of SIRT1 and PGC-1α,elevated protein expression of p-AMPK,and reduced protein expression of NF-κB.Consistent with the in-vivo results,the in-vitro results indicated that SJZT improved exercise performance by modulating p38 MAPK signaling.Conclusion:These results suggest that SJZT improves exercise performance in mice by a mechanism that may be related to the inhibition of the p38 MAPK signaling pathway.展开更多
对流云是南方人工增雨开发利用空中云水资源的重要对象,结构复杂多变;通过数值模式合理评估催化作业过程,进而研究其催化机制,是建立和改进催化作业技术的必要途径,也是评估实际人工增雨作业效果的有效手段。利用耦合了碘化银(AgI)催化...对流云是南方人工增雨开发利用空中云水资源的重要对象,结构复杂多变;通过数值模式合理评估催化作业过程,进而研究其催化机制,是建立和改进催化作业技术的必要途径,也是评估实际人工增雨作业效果的有效手段。利用耦合了碘化银(AgI)催化的WRF(Weather Research and Forecasting)模式,对2021年5月4日福建古田人工增雨随机化试验个例开展催化模拟,分析AgI核化机制、催化对云系宏微观特征、降水机制的影响以及增雨效果评估。结果显示,AgI播撒后呈带状扩散,催化前期(09:00—11:00)(世界时,下同)地面降水增量缓慢增加;随后(11:00—13:00)降水增幅加大并出现剧烈波动;13:00后降水增量以负值为主。AgI主要核化机制为凝华核化,核化持续约40 min。AgI播撒后主要通过凝华核化使冰晶数浓度大幅增加(增量约3~9个·L^(-1)),增长的冰晶大部分转化为雪晶,再通过雪晶融化增加云中雨滴质量浓度。此次过程催化影响时间持续约4 h,催化部位绝对增雨量约-0.78~1.24 mm,增雨率约-8.3%~12.1%,总降水增量为4.64×10^(5) t,增雨效果显著。展开更多
基金supported by Basic Scientific Research Fund of Education Department of Liaoning Province,China(LJ212410161100 to Z-L.L.)Natural Science Foundation of Liaoning Province,China(2022-MS-326 to Z-L.L.and 2021-BS-294 to B.W.)+1 种基金Dengfeng project of Dalian medical discipline priority(2022ZZ258 and 2023ZZ025 to B.W.)China Foundation For Youth Entrepreneurship And Employment(P24041687730 to L-N.X.).
文摘Background:Si-Jun-Zi-Tang(SJZT),a traditional Chinese herbal formula,is designed to boost vitality and promote spleen health,bringing numerous benefits to the body.It has indicated that herbal remedies have a potential link with enhanced exercise performance,reducing the risk of various chronic diseases.In this study,we will observe the impact of SJZT on the exercise performance of mice and explore the underlying mechanism.Methods:In this study,we used the steam distillation method to obtain the extract of SJZT.Mice were given pure water,low and high doses of SJZT respectively for 4 weeks,followed by the assessment of exercise performance(Forelimb Grip Strength Test,Exhausted Swimming Training,Exhausted Treadmill Running Test,and 10-min Swimming Test).Subsequently,serum and urine biochemical indices,glycogen content,liver mRNA,and protein expression were analyzed after the mice completed 6 weeks of treadmill training.In the cellular experiments,we used the HepG2 cell line treated with SJZT post-gavage serum to mimic the in-vivo SJZT-administered mouse model,and used Western blot,qPCR,and other experimental techniques to thoroughly investigate the potential mechanism of SJZT to enhance exercise performance.Results:Compared with the Vehicle group,the SJZT groups showed increased lactate clearance and elevated liver glycogen levels.In terms of exercise performance,grip strength,exercise capacity,and lactate clearance were increased,and lactate production was decreased in the SJZT groups.In comparison to the Vehicle group,the SJZT groups exhibited decreased RNA and protein expression of p-p38 MAPK,increased RNA and protein expression of SIRT1 and PGC-1α,elevated protein expression of p-AMPK,and reduced protein expression of NF-κB.Consistent with the in-vivo results,the in-vitro results indicated that SJZT improved exercise performance by modulating p38 MAPK signaling.Conclusion:These results suggest that SJZT improves exercise performance in mice by a mechanism that may be related to the inhibition of the p38 MAPK signaling pathway.
文摘对流云是南方人工增雨开发利用空中云水资源的重要对象,结构复杂多变;通过数值模式合理评估催化作业过程,进而研究其催化机制,是建立和改进催化作业技术的必要途径,也是评估实际人工增雨作业效果的有效手段。利用耦合了碘化银(AgI)催化的WRF(Weather Research and Forecasting)模式,对2021年5月4日福建古田人工增雨随机化试验个例开展催化模拟,分析AgI核化机制、催化对云系宏微观特征、降水机制的影响以及增雨效果评估。结果显示,AgI播撒后呈带状扩散,催化前期(09:00—11:00)(世界时,下同)地面降水增量缓慢增加;随后(11:00—13:00)降水增幅加大并出现剧烈波动;13:00后降水增量以负值为主。AgI主要核化机制为凝华核化,核化持续约40 min。AgI播撒后主要通过凝华核化使冰晶数浓度大幅增加(增量约3~9个·L^(-1)),增长的冰晶大部分转化为雪晶,再通过雪晶融化增加云中雨滴质量浓度。此次过程催化影响时间持续约4 h,催化部位绝对增雨量约-0.78~1.24 mm,增雨率约-8.3%~12.1%,总降水增量为4.64×10^(5) t,增雨效果显著。
