Warm-sector heavy rainfall(WR),shear-line heavy rainfall(SR),and frontal heavy rainfall(FR)are three types of rainfall that frequently occur during the pre-summer rainy season in south China.In this research,we invest...Warm-sector heavy rainfall(WR),shear-line heavy rainfall(SR),and frontal heavy rainfall(FR)are three types of rainfall that frequently occur during the pre-summer rainy season in south China.In this research,we investigated the differences in microphysical characteristics of heavy rainfall events during the period of 10-15 May 2022 based on the combined observations from 11 S-band polarimetric radars in south China.The conclusions are as follows:(1)WR has the highest radar echo top height,the strongest radar echo at all altitudes,the highest lightning density,and the most active ice-phase process,which suggests that the convection is the most vigorous in the WR,moderate in the FR,and the weakest in the SR.(2)Three types of rainfall are all marine-type precipitation,the massweighted mean diameter(Dm,mm)and the intercept parameter(Nw,mm^(-1) m^(-3))of the raindrops in the WR are the largest.(3)The WR possesses the highest proportion of graupel compared with the FR and SR,and stronger updrafts and more abundant water vapor supply may lead to larger raindrops during the melting and collision-coalescence processes.(4)Over all the heights,liquid and ice water content in the WR are higher than those in the SR and FR,the ratio of ice to liquid water content in the WR is as high as 27%when ZH exceeds 50 dBZ,definitely higher than that in the SR and FR,indicating that the active ice-phase process existing in the WR is conducive to the formation of heavy rainfall.展开更多
Objective:To detect the expression levels of programmed cell death 1(PD‑1)and its ligand PD‑L1,regulatory cell(Treg)and T helper cell 17(Th17)specific nuclear transcription factors forkhead box protein 3(Foxp3)and the...Objective:To detect the expression levels of programmed cell death 1(PD‑1)and its ligand PD‑L1,regulatory cell(Treg)and T helper cell 17(Th17)specific nuclear transcription factors forkhead box protein 3(Foxp3)and the retinoic acid‑related orphan nuclear receptorgammat(RORγc)in the placenta of normal pregnant women and in preeclamptic(PE)women,and to study the relationship between their differential expression and the development of PE.Methods:Between March 2021 and March 2022,40 patients with pre‑eclampsia who were treated at the Obstetrical Department in Hainan Hospital were selected,including 20 cases of mild(PE)and 20 cases of severe(sPE),and 20 normal pregnant women with singleton cesarean section in the same period were selected to collect placental tissue.PD‑1,PD‑L1,Foxp3 and RORγc in placenta were detected by qRT‑PCR and Western blot.Results:(1)The results of qRT‑PCR assay showed that the mRNA expression of PD‑1,PD‑L1,Foxp3 protein decreased significantly compared with the control group.The changes in PD‑1,PD‑L1 and Foxp3 became more obvious as the disease progressed(P<0.05);The mRNA expression of RORγc increased significantly compared with the control group.The changes of RORγc mRNA became more obviousas as the disease progressed(P<0.05);(2)The results of Western blot assay show,compared with the normal group,the protein expression of PD‑1,PD‑L1,Foxp3 mRNA decreased significantly.With the aggravation of the disease,the changes of PD‑1 and PD‑L1 became more obvious,with significant difference(P<0.05);Compared with the normal group,the protein expression of RORγc increased significantly.With the aggravation of the disease,the changes of RORγc protein became more obvious with significant difference(P<0.05).Conclusion:PD‑1/PD‑Ll participates in the occurrence and development of preeclampsia by regulating Treg/Th17 immune balance.展开更多
目的:外固定支架和钢板内固定作为桡骨远端粉碎性骨折的常用治疗方法,在临床上各有利弊。系统评价外固定支架与钢板内固定治疗粉碎性桡骨远端骨折的临床疗效及安全性,为桡骨远端骨折中西医结合诊疗指南研制提供论证依据。方法:系统检索P...目的:外固定支架和钢板内固定作为桡骨远端粉碎性骨折的常用治疗方法,在临床上各有利弊。系统评价外固定支架与钢板内固定治疗粉碎性桡骨远端骨折的临床疗效及安全性,为桡骨远端骨折中西医结合诊疗指南研制提供论证依据。方法:系统检索PubMed、Web of Science、Embase、Cochrane Library、中国知网、中国生物医学文献数据库、维普和万方数据库,纳入2013年10月至2023年10月发表的关于外固定支架和钢板内固定治疗粉碎性桡骨远端骨折的随机对照试验文献,按照纳入标准和排除标准筛选文献,使用Review Manager进行文献质量评价和Meta分析。结果:(1)纳入8篇文献,其中中文文献4篇,英文文献4篇、总样本量648例,外固定支架组328例,钢板内固定组320例;(2)术后3个月,钢板内固定组的背伸、掌屈、旋后范围优于外固定支架组;术后12个月,钢板内固定组的握力、掌倾角、掌屈、旋前和旋后范围优于外固定支架组;钢板内固定组在术后感染方面优于外固定支架组,其余结局指标两组差异均无显著性意义。结论:现有8项证据表明,在粉碎性桡骨远端骨折的治疗方式选择上,外固定支架与切开钢板内固定都有良好的治疗效果,综合其他因素钢板内固定更胜一筹,但是对于一些高度严重的粉碎性桡骨远端骨折、骨质较差、严重污染的开放性骨折以及软组织肿胀而无法进行切开手术的特殊患者,外固定支架才是首选。此次研究结果具有局限性,未来还需要开展更多高质量、大样本、多中心的随机对照试验研究,另外需重视远期疗效、其他次要指标的观察,补充优化当前研究结果。展开更多
Single crystalline sificon films are transferred on to a glass substrate by the sm art-cut technique.which bas ed on H^(+)ions im plan tation.anodic bonding and layer transfer.Structures of the resulting thin film sil...Single crystalline sificon films are transferred on to a glass substrate by the sm art-cut technique.which bas ed on H^(+)ions im plan tation.anodic bonding and layer transfer.Structures of the resulting thin film silicon on glass (SOG)are characterized by transmission-electron microscopy,scanning electron microscopy an d Raman spectroscopy.The resu/ts show that S0G substrates fabricated by the smart-cut have advantages of steep top Si/glass interrace an d good monocrystalline Si quaiity.The HalLefect measurement indicates that the single crystalline SOG substrates have a better elec trival property compared with polyerystailine silicon S0G substrates.展开更多
【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探...【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探针,优化反应体系中引物和探针的浓度,建立Taq Man RT-qPCR检测方法,并通过特异性、灵敏性和重复性的结果以及临床应用对该方法进行评价。【结果】该方法可特异性扩增PoRV核酸,最低检出限度为27.0 copies·μL^(-1),灵敏度高于普通RT-PCR 100倍;与猪流行性腹泻病毒(porcine epidemicdiarrheavirus,PEDV)、猪德尔塔冠状病毒(porcinedeltacoronavirus,PDCoV)、猪传染性胃肠炎病毒(transmissible gastroenteritis of swine, TGEV)核酸均无交叉反应;组内和组间变异系数均小于1.10%,重复性好。151份疑似PoRV的临床样品使用RT-qPCR进行检测,结果显示检出率为42.38%(64/151),优于常规RT-PCR的检出率(33.11%,50/151)。【结论】本研究基于猪A群轮状病毒VP6基因,建立了适用于PoRV A检测及其流行病学调查的Taq Man实时荧光定量PCR检测方法,具有灵敏度高、特异性强、重复性好等优势,为猪轮状病毒检测和流行病学调查提供了技术手段。展开更多
基金National Natural Science Foundation of China(U2242203,41975138,41905047,42030610)the High-level Science and Technology Journals Projects of Guangdong Province(2021B1212020016)+2 种基金Natural Science Foundation of Guangdong Province(2019A1515010814,2021A1515011415)Science and Technology Research Project of Guangdong Meteorological Bureau(GRMC2020M01)the Joint Research Project for Meteorological Capacity Improvement(22NLTSQ003)。
文摘Warm-sector heavy rainfall(WR),shear-line heavy rainfall(SR),and frontal heavy rainfall(FR)are three types of rainfall that frequently occur during the pre-summer rainy season in south China.In this research,we investigated the differences in microphysical characteristics of heavy rainfall events during the period of 10-15 May 2022 based on the combined observations from 11 S-band polarimetric radars in south China.The conclusions are as follows:(1)WR has the highest radar echo top height,the strongest radar echo at all altitudes,the highest lightning density,and the most active ice-phase process,which suggests that the convection is the most vigorous in the WR,moderate in the FR,and the weakest in the SR.(2)Three types of rainfall are all marine-type precipitation,the massweighted mean diameter(Dm,mm)and the intercept parameter(Nw,mm^(-1) m^(-3))of the raindrops in the WR are the largest.(3)The WR possesses the highest proportion of graupel compared with the FR and SR,and stronger updrafts and more abundant water vapor supply may lead to larger raindrops during the melting and collision-coalescence processes.(4)Over all the heights,liquid and ice water content in the WR are higher than those in the SR and FR,the ratio of ice to liquid water content in the WR is as high as 27%when ZH exceeds 50 dBZ,definitely higher than that in the SR and FR,indicating that the active ice-phase process existing in the WR is conducive to the formation of heavy rainfall.
基金Medical and Health Research Project of Hainan Province(21A200131)。
文摘Objective:To detect the expression levels of programmed cell death 1(PD‑1)and its ligand PD‑L1,regulatory cell(Treg)and T helper cell 17(Th17)specific nuclear transcription factors forkhead box protein 3(Foxp3)and the retinoic acid‑related orphan nuclear receptorgammat(RORγc)in the placenta of normal pregnant women and in preeclamptic(PE)women,and to study the relationship between their differential expression and the development of PE.Methods:Between March 2021 and March 2022,40 patients with pre‑eclampsia who were treated at the Obstetrical Department in Hainan Hospital were selected,including 20 cases of mild(PE)and 20 cases of severe(sPE),and 20 normal pregnant women with singleton cesarean section in the same period were selected to collect placental tissue.PD‑1,PD‑L1,Foxp3 and RORγc in placenta were detected by qRT‑PCR and Western blot.Results:(1)The results of qRT‑PCR assay showed that the mRNA expression of PD‑1,PD‑L1,Foxp3 protein decreased significantly compared with the control group.The changes in PD‑1,PD‑L1 and Foxp3 became more obvious as the disease progressed(P<0.05);The mRNA expression of RORγc increased significantly compared with the control group.The changes of RORγc mRNA became more obviousas as the disease progressed(P<0.05);(2)The results of Western blot assay show,compared with the normal group,the protein expression of PD‑1,PD‑L1,Foxp3 mRNA decreased significantly.With the aggravation of the disease,the changes of PD‑1 and PD‑L1 became more obvious,with significant difference(P<0.05);Compared with the normal group,the protein expression of RORγc increased significantly.With the aggravation of the disease,the changes of RORγc protein became more obvious with significant difference(P<0.05).Conclusion:PD‑1/PD‑Ll participates in the occurrence and development of preeclampsia by regulating Treg/Th17 immune balance.
文摘目的:外固定支架和钢板内固定作为桡骨远端粉碎性骨折的常用治疗方法,在临床上各有利弊。系统评价外固定支架与钢板内固定治疗粉碎性桡骨远端骨折的临床疗效及安全性,为桡骨远端骨折中西医结合诊疗指南研制提供论证依据。方法:系统检索PubMed、Web of Science、Embase、Cochrane Library、中国知网、中国生物医学文献数据库、维普和万方数据库,纳入2013年10月至2023年10月发表的关于外固定支架和钢板内固定治疗粉碎性桡骨远端骨折的随机对照试验文献,按照纳入标准和排除标准筛选文献,使用Review Manager进行文献质量评价和Meta分析。结果:(1)纳入8篇文献,其中中文文献4篇,英文文献4篇、总样本量648例,外固定支架组328例,钢板内固定组320例;(2)术后3个月,钢板内固定组的背伸、掌屈、旋后范围优于外固定支架组;术后12个月,钢板内固定组的握力、掌倾角、掌屈、旋前和旋后范围优于外固定支架组;钢板内固定组在术后感染方面优于外固定支架组,其余结局指标两组差异均无显著性意义。结论:现有8项证据表明,在粉碎性桡骨远端骨折的治疗方式选择上,外固定支架与切开钢板内固定都有良好的治疗效果,综合其他因素钢板内固定更胜一筹,但是对于一些高度严重的粉碎性桡骨远端骨折、骨质较差、严重污染的开放性骨折以及软组织肿胀而无法进行切开手术的特殊患者,外固定支架才是首选。此次研究结果具有局限性,未来还需要开展更多高质量、大样本、多中心的随机对照试验研究,另外需重视远期疗效、其他次要指标的观察,补充优化当前研究结果。
基金Supported by the National Natural Science Foundation of China under Grant No 90101012
文摘Single crystalline sificon films are transferred on to a glass substrate by the sm art-cut technique.which bas ed on H^(+)ions im plan tation.anodic bonding and layer transfer.Structures of the resulting thin film silicon on glass (SOG)are characterized by transmission-electron microscopy,scanning electron microscopy an d Raman spectroscopy.The resu/ts show that S0G substrates fabricated by the smart-cut have advantages of steep top Si/glass interrace an d good monocrystalline Si quaiity.The HalLefect measurement indicates that the single crystalline SOG substrates have a better elec trival property compared with polyerystailine silicon S0G substrates.
文摘【目的】建立一种猪A群轮状病毒(porcine rotavirus group A, PoRV A)快速检测方法,用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒(PoRVA)VP6基因序列(登录号MT025937.1、OP978242.1、PP566178.1)设计特异性引物和探针,优化反应体系中引物和探针的浓度,建立Taq Man RT-qPCR检测方法,并通过特异性、灵敏性和重复性的结果以及临床应用对该方法进行评价。【结果】该方法可特异性扩增PoRV核酸,最低检出限度为27.0 copies·μL^(-1),灵敏度高于普通RT-PCR 100倍;与猪流行性腹泻病毒(porcine epidemicdiarrheavirus,PEDV)、猪德尔塔冠状病毒(porcinedeltacoronavirus,PDCoV)、猪传染性胃肠炎病毒(transmissible gastroenteritis of swine, TGEV)核酸均无交叉反应;组内和组间变异系数均小于1.10%,重复性好。151份疑似PoRV的临床样品使用RT-qPCR进行检测,结果显示检出率为42.38%(64/151),优于常规RT-PCR的检出率(33.11%,50/151)。【结论】本研究基于猪A群轮状病毒VP6基因,建立了适用于PoRV A检测及其流行病学调查的Taq Man实时荧光定量PCR检测方法,具有灵敏度高、特异性强、重复性好等优势,为猪轮状病毒检测和流行病学调查提供了技术手段。
